Restriction map for pF1KB5834
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        Alw44I      
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AvrII       BalI        BbrPI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BsaBI       BsaWI       BseAI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        EcoNI       
EcoO65I     EcoRI       EcoRV       FbaI        HincII      
HindII      HpaI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfcI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
TfiI        Tru9I       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AspI          634  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI           69  GAAGAC               GAAGAC(2/6)
BbvCI         525  GCTGAGG              CCTCAGC(-5/-2)
BfaI         1397  CTAG                 C^TAG
BpuAI          69  GAAGAC               GAAGAC(2/6)
BsaAI         520  TACGTG               [CT]AC^GT[GA]
BsaI          235  GAGACC               GGTCTC(1/5)
BspMI         293  ACCTGC               ACCTGC(4/8)
BsrBI        1362  GAGCGG               CCGCTC(-3/-3)
BsrDI         912  CATTGC               GCAATG(2/0)
BstAPI       1035  GCAGCAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EarI         1359  GAAGAG               CTCTTC(1/4)
EcoT22I      1154  ATGCAT               ATGCA^T
FauI         1364  GCGGG                CCCGC(4/6)
FseI          657  GGCCGGCC             GGCCGG^CC
HindIII       400  AAGCTT               A^AGCTT
Ksp632I      1359  GAAGAG               CTCTTC(1/4)
MaeI         1397  CTAG                 C^TAG
MslI         1157  CATGGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          658  GCCGGC               GCC^GGC
NgoMIV        658  GCCGGC               G^CCGGC
NsiI         1154  ATGCAT               ATGCA^T
NspI         1026  GCATGC               [GA]CATG^[CT]
PaeR7I        118  CTCGAG               C^TCGAG
PflFI         634  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI          56  CCAAGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1416  GACTGGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         366  CAGCTG               CAG^CTG
SapI         1359  GAAGAGC              GCTCTTC(1/4)
SexAI        1296  ACCTGGT              A^CC[AT]GGT
SmlI          118  CTCGAG               C^T[CT][GA]AG
SphI         1026  GCATGC               GCATG^C
Tsp45I        226  GTCAC                ^GT[GC]AC
Tsp509I       461  AATT                 ^AATT
Tth111I       634  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I         56  CCAAGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1396  TCTAGA               T^CTAGA
XcmI          589  CCACTCCTGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          118  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AcyI          253  GGCGCC               G[GA]^CG[CT]C
              378  GGCGCC              
AhdI          307  GACGCGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              958  GACAGCACGTC         
ApaI          566  GGGCCC               GGGCC^C
             1278  GGGCCC              
AspEI         307  GACGCGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              958  GACAGCACGTC         
AvaII          53  GGACC                G^G[AT]CC
             1353  GGTCC               
AviII         908  TGCGCA               TGC^GCA
             1029  TGCGCA              
BamHI         454  GGATCC               G^GATCC
             1074  GGATCC              
BbeI          253  GGCGCC               GGCGC^C
              378  GGCGCC              
BglI          506  GCCTGCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              671  GCCCCTCAGGC         
BlpI          280  GCTCAGC              GC^T[ACGT]AGC
              889  GCTGAGC             
Bpu1102I      280  GCTCAGC              GC^T[ACGT]AGC
              889  GCTGAGC             
BsaHI         253  GGCGCC               G[GA]^CG[CT]C
              378  GGCGCC              
BseRI         862  CTCCTC               GAGGAG(10/8)
             1183  GAGGAG              
BsiEI         302  CGGCCG               CG[GA][CT]^CG
              660  CGGCCG              
BsmAI         235  GAGAC                GTCTC(1/5)
              736  GAGAC               
Bsp1407I      434  TGTACA               T^GTACA
             1343  TGTACA              
BsrFI         658  GCCGGC               [GA]^CCGG[CT]
              749  ACCGGC              
BsrGI         434  TGTACA               T^GTACA
             1343  TGTACA              
BstF5I        744  CATCC                GGATG(2/0)
             1152  GGATG               
BstYI         454  GGATCC               [GA]^GATC[CT]
             1074  GGATCC              
CelII         280  GCTCAGC              GC^T[ACGT]AGC
              889  GCTGAGC             
Cfr10I        658  GCCGGC               [GA]^CCGG[CT]
              749  ACCGGC              
EagI          302  CGGCCG               C^GGCCG
              660  CGGCCG              
Eam1105I      307  GACGCGCCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              958  GACAGCACGTC         
EclXI         302  CGGCCG               C^GGCCG
              660  CGGCCG              
Eco52I        302  CGGCCG               C^GGCCG
              660  CGGCCG              
EcoT14I       953  CCTTGG               C^C[AT][AT]GG
             1148  CCAAGG              
FokI          744  CATCC                GGATG(9/13)
             1152  GGATG               
FspI          908  TGCGCA               TGC^GCA
             1029  TGCGCA              
Hin1I         253  GGCGCC               G[GA]^CG[CT]C
              378  GGCGCC              
KasI          253  GGCGCC               G^GCGCC
              378  GGCGCC              
MaeII         521  ACGT                 A^CGT
              964  ACGT                
MaeIII        226  GTCAC                ^GT[ACGT]AC
              469  GTAAC               
MflI          454  GGATCC               [GA]^GATC[CT]
             1074  GGATCC              
MspA1I        366  CAGCTG               C[AC]G^C[GT]G
              666  CCGCTG              
NarI          253  GGCGCC               GG^CGCC
              378  GGCGCC              
SfoI          253  GGCGCC               GGC^GCC
              378  GGCGCC              
SspBI         434  TGTACA               T^GTACA
             1343  TGTACA              
StyI          953  CCTTGG               C^C[AT][AT]GG
             1148  CCAAGG              
XhoII         454  GGATCC               [GA]^GATC[CT]
             1074  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AocI        Aor51HI     AspHI       AvaI        
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstNI       BstUI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        Eco47III    Eco81I      EcoO109I    
EcoRII      Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MboI        MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TseI        TspRI       TthHB8I     
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