Restriction map for
pF1KB5834
(Circular) (Six-base) MAPSORT of: pF1KB5834.seq Check: 2338 from: 1 to: 4556
pF1KB5834 4556 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 410 410
Size: 4556
AccI GT'mk_AC
Cuts at: 1594 1594
Size: 4556
Acc65I G'GTAC_C
Cuts at: 1572 1572
Size: 4556
AclI AA'CG_TT
Cuts at: 4396 4396
Size: 4556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 2381 2813 3560 3828 1174
Size: 1207 432 747 268 1902
Fragments arranged by size:
1902 1207 747 432 268
AfeI AGC'GCT
Cuts at: 154 394 607 154
Size: 240 213 4103
Fragments arranged by size:
4103 240 213
AflIII A'CryG_T
Cuts at: 1908 3012 3989 1908
Size: 1104 977 2475
Fragments arranged by size:
2475 1104 977
AgeI A'CCGG_T
Cuts at: 2944 2944
Size: 4556
AhdI GACnn_n'nnGTC
Cuts at: 420 1071 420
Size: 651 3905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1287 1287
Size: 4556
AlwNI CAG_nnn'CTG
Cuts at: 447 690 790 1152 1861 3428 447
Size: 243 100 362 709 1567 1575
Fragments arranged by size:
1575 1567 709 362 243 100
ApaI G_GGCC'C
Cuts at: 678 1390 678
Size: 712 3844
ApaLI G'TGCA_C
Cuts at: 3326 3326
Size: 4556
ApoI r'AATT_y
Cuts at: 1560 3909 4165 1560
Size: 2349 256 1951
Fragments arranged by size:
2349 1951 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4556
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
AvaI C'yCGr_G
Cuts at: 226 1247 1280 1576 226
Size: 1021 33 296 3206
Fragments arranged by size:
3206 1021 296 33
BamHI G'GATC_C
Cuts at: 562 1182 1581 562
Size: 620 399 3537
Fragments arranged by size:
3537 620 399
BanI G'GyrC_C
Cuts at: 361 486 1271 1572 1986 2234 2269 361
Size: 125 785 301 414 248 35 2648
Fragments arranged by size:
2648 785 414 301 248 125 35
BanII G_rGCy'C
Cuts at: 256 277 359 678 971 1004 1256 1307 1390
Size: 21 82 319 293 33 252 51 83
Cuts at: 1390 1502 1546 1570 2600 256
Size: 112 44 24 1030 2212
Fragments arranged by size:
2212 1030 319 293 252 112 83 82
51 44 33 24 21
BbeI G_GCGC'C
Cuts at: 365 490 1990 2238 365
Size: 125 1500 248 2683
Fragments arranged by size:
2683 1500 248 125
BbsI GAAGACnn'nnnn_
Cuts at: 184 184
Size: 4556
BbvCI CC'TCA_GC
Cuts at: 634 634
Size: 4556
BciVI GTATCCnnnnn_n'
Cuts at: 2454 3215 4556 2454
Size: 761 1341 2454
Fragments arranged by size:
2454 1341 761
BclI T'GATC_A
Cuts at: 2076 2076
Size: 4556
BfrBI ATG'CAT
Cuts at: 1264 1264
Size: 4556
BglI GCCn_nnn'nGGC
Cuts at: 620 785 620
Size: 165 4391
BglII A'GATC_T
Cuts at: 2071 4108 2071
Size: 2037 2519
BlpI GC'TnA_GC
Cuts at: 389 998 1668 389
Size: 609 670 3277
Fragments arranged by size:
3277 670 609
Bme1580I G_kGCm'C
Cuts at: 678 893 1390 2181 2274 3330 678
Size: 215 497 791 93 1056 1904
Fragments arranged by size:
1904 1056 791 497 215 93
BmgBI CAC'GTC
Cuts at: 1073 1073
Size: 4556
BmrI ACTGGGnnnn_n'
Cuts at: 1210 1535 1768 1849 2184 4328 1210
Size: 325 233 81 335 2144 1438
Fragments arranged by size:
2144 1438 335 325 233 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2923 2923
Size: 4556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 550 712 541
Size: 9 162 4385
Fragments arranged by size:
4385 162 9
Bpu10I CC'TnA_GC
Cuts at: 353 506 634 1079 353
Size: 153 128 445 3830
Fragments arranged by size:
3830 445 153 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1732 2611 3103 3401 1732
Size: 879 492 298 2887
Fragments arranged by size:
2887 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 337 4260 337
Size: 3923 633
BsaAI yAC'GTr
Cuts at: 630 1909 630
Size: 1279 3277
BsaBI GATnn'nnATC
Cuts at: 6 2070 4107 6
Size: 2064 2037 455
Fragments arranged by size:
2064 2037 455
BsaHI Gr'CG_yC
Cuts at: 362 487 1987 2235 2937 4433 362
Size: 125 1500 248 702 1496 485
Fragments arranged by size:
1500 1496 702 485 248 125
BsaWI w'CCGG_w
Cuts at: 1745 2266 2944 3218 3365 3776 1745
Size: 521 678 274 147 411 2525
Fragments arranged by size:
2525 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1079 1109 1079
Size: 30 4526
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 961 1306 961
Size: 345 4211
BseYI C'CCAG_C
Cuts at: 984 1389 1487 1981 2739 3316 3812 984
Size: 405 98 494 758 577 496 1728
Fragments arranged by size:
1728 758 577 496 494 405 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 460 604 449
Size: 11 144 4401
Fragments arranged by size:
4401 144 11
BsiEI CG_ry'CG
Cuts at: 104 413 771 2098 2144 3352 104
Size: 309 358 1327 46 1208 1308
Fragments arranged by size:
1327 1308 1208 358 309 46
BsiHKAI G_wGCw'C
Cuts at: 194 256 277 971 1307 1357 1570 2348 2538
Size: 62 21 694 336 50 213 778 190
Cuts at: 2538 3330 194
Size: 792 1420
Fragments arranged by size:
1420 792 778 694 336 213 190 62
50 21
Bsp1286I G_dGCh'C
Cuts at: 194 256 277 359 678 893 971 1004 1256
Size: 62 21 82 319 215 78 33 252
Cuts at: 1256 1307 1357 1390 1502 1546 1570 2181 2274
Size: 51 50 33 112 44 24 611 93
Cuts at: 2274 2348 2538 2600 3330 194
Size: 74 190 62 730 1420
Fragments arranged by size:
1420 730 611 319 252 215 190 112
93 82 78 74 62 62 51 50
44 33 33 24 21
BspEI T'CCGG_A
Cuts at: 3776 3776
Size: 4556
BspHI T'CATG_A
Cuts at: 3732 3732
Size: 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 410 1606 1820 2122 2503 410
Size: 1196 214 302 381 2463
Fragments arranged by size:
2463 1196 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1472 2848 2902 4386 4551 50
Size: 1422 1376 54 1484 165 55
Fragments arranged by size:
1484 1422 1376 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1019 2468 1019
Size: 1449 3107
BsrFI r'CCGG_y
Cuts at: 766 857 2554 2735 2944 766
Size: 91 1697 181 209 2378
Fragments arranged by size:
2378 1697 209 181 91
BsrGI T'GTAC_A
Cuts at: 542 1451 542
Size: 909 3647
BssSI C'ACGA_G
Cuts at: 2827 3185 2827
Size: 358 4198
BstAPI GCAn_nnn'nTGC
Cuts at: 1149 1933 1149
Size: 784 3772
BstBI TT'CG_AA
Cuts at: 2917 2917
Size: 4556
BstYI r'GATC_y
Cuts at: 1 562 1182 1581 2048 2071 2406 2652 3664
Size: 561 620 399 467 23 335 246 1012
Cuts at: 3664 3750 3762 4108 1
Size: 86 12 346 449
Fragments arranged by size:
1012 620 561 467 449 399 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 783 1202 1479 783
Size: 419 277 3860
Fragments arranged by size:
3860 419 277
BtgI C'CryG_G
Cuts at: 107 210 338 668 107
Size: 103 128 330 3995
Fragments arranged by size:
3995 330 128 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2506 2687 2850 2506
Size: 181 163 4212
Fragments arranged by size:
4212 181 163
BtsI GCAGTG_nn'
Cuts at: 125 365 383 1428 1799 125
Size: 240 18 1045 371 2882
Fragments arranged by size:
2882 1045 371 240 18
DraI TTT'AAA
Cuts at: 1555 1555
Size: 4556
DrdI GACnn_nn'nnGTC
Cuts at: 2262 3120 2262
Size: 858 3698
EaeI y'GGCC_r
Cuts at: 200 264 410 735 768 2141 2315 2706 2733
Size: 64 146 325 33 1373 174 391 27
Cuts at: 2733 4078 200
Size: 1345 678
Fragments arranged by size:
1373 1345 678 391 325 174 146 64
33 27
EagI C'GGCC_G
Cuts at: 410 768 2141 410
Size: 358 1373 2825
Fragments arranged by size:
2825 1373 358
EarI CTCTTCn'nnn_
Cuts at: 1462 2579 2789 1462
Size: 1117 210 3229
Fragments arranged by size:
3229 1117 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 364 2764 3074 3220 4366 364
Size: 2400 310 146 1146 554
Fragments arranged by size:
2400 1146 554 310 146
EcoICRI GAG'CTC
Cuts at: 254 275 969 1305 1568 254
Size: 21 694 336 263 3242
Fragments arranged by size:
3242 694 336 263 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 550 712 1174 2381 2813 3560 3828 541
Size: 9 162 462 1207 432 747 268 1269
Fragments arranged by size:
1269 1207 747 462 432 268 162 9
EcoO109I rG'GnC_Cy
Cuts at: 674 787 1206 1230 1386 1475 1695 674
Size: 113 419 24 156 89 220 3535
Fragments arranged by size:
3535 419 220 156 113 89 24
EcoRI G'AATT_C
Cuts at: 1560 1560
Size: 4556
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 151 2575 151
Size: 2424 2132
FseI GG_CCGG'CC
Cuts at: 770 770
Size: 4556
FspI TGC'GCA
Cuts at: 1018 1139 2337 1018
Size: 121 1198 3237
Fragments arranged by size:
3237 1198 121
HaeII r_GCGC'y
Cuts at: 156 365 396 490 609 1990 2238 3260 4239
Size: 209 31 94 119 1381 248 1022 979
Cuts at: 4239 156
Size: 473
Fragments arranged by size:
1381 1022 979 473 248 209 119 94
31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 406 438 1057 1089 2398 2428 2430 2460 406
Size: 32 619 32 1309 30 2 30 2502
Fragments arranged by size:
2502 1309 619 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1595 1595
Size: 4556
HindIII A'AGCT_T
Cuts at: 508 508
Size: 4556
KasI G'GCGC_C
Cuts at: 361 486 1986 2234 361
Size: 125 1500 248 2683
Fragments arranged by size:
2683 1500 248 125
KpnI G_GTAC'C
Cuts at: 1576 1576
Size: 4556
MluI A'CGCG_T
Cuts at: 3989 3989
Size: 4556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3227 3411 3227
Size: 184 4372
MscI TGG'CCA
Cuts at: 2317 2317
Size: 4556
MslI CAynn'nnrTG
Cuts at: 1269 2545 2672 1269
Size: 1276 127 3153
Fragments arranged by size:
3153 1276 127
MspA1I CmG'CkG
Cuts at: 476 776 1667 1981 2341 3354 3599 476
Size: 300 891 314 360 1013 245 1433
Fragments arranged by size:
1433 1013 891 360 314 300 245
NaeI GCC'GGC
Cuts at: 768 2737 768
Size: 1969 2587
NarI GG'CG_CC
Cuts at: 362 487 1987 2235 362
Size: 125 1500 248 2683
Fragments arranged by size:
2683 1500 248 125
NcoI C'CATG_G
Cuts at: 107 107
Size: 4556
NgoMIV G'CCGG_C
Cuts at: 766 2735 766
Size: 1969 2587
NsiI A_TGCA'T
Cuts at: 1266 1266
Size: 4556
NspI r_CATG'y
Cuts at: 1138 1609 2109 3016 1138
Size: 471 500 907 2678
Fragments arranged by size:
2678 907 500 471
PasI CC'CwG_GG
Cuts at: 870 1227 1383 870
Size: 357 156 4043
Fragments arranged by size:
4043 357 156
PciI A'CATG_T
Cuts at: 3012 3012
Size: 4556
PflMI CCAn_nnn'nTGG
Cuts at: 170 170
Size: 4556
PmeI GTTT'AAAC
Cuts at: 1555 1555
Size: 4556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4524
PshAI GACnn'nnGTC
Cuts at: 1528 1528
Size: 4556
PspOMI G'GGCC_C
Cuts at: 674 1386 674
Size: 712 3844
PspXI vC'TCGA_Gb
Cuts at: 226 226
Size: 4556
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 526 558 526
Size: 32 4524
PstI C_TGCA'G
Cuts at: 1603 2288 1603
Size: 685 3871
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4556
PvuII CAG'CTG
Cuts at: 476 1981 2341 476
Size: 1505 360 2691
Fragments arranged by size:
2691 1505 360
RsrII CG'GwC_CG
Cuts at: 2751 2751
Size: 4556
SacI G_AGCT'C
Cuts at: 256 277 971 1307 1570 256
Size: 21 694 336 263 3242
Fragments arranged by size:
3242 694 336 263 21
SalI G'TCGA_C
Cuts at: 1593 1593
Size: 4556
SapI GCTCTTCn'nnn_
Cuts at: 1462 2579 2789 1462
Size: 1117 210 3229
Fragments arranged by size:
3229 1117 210
SbfI CC_TGCA'GG
Cuts at: 1603 1603
Size: 4556
ScaI AGT'ACT
Cuts at: 4073 4073
Size: 4556
SexAI A'CCwGG_T
Cuts at: 1404 1404
Size: 4556
SfcI C'TryA_G
Cuts at: 33 1599 2284 3277 3468 33
Size: 1566 685 993 191 1121
Fragments arranged by size:
1566 1121 993 685 191
SfoI GGC'GCC
Cuts at: 363 488 1988 2236 363
Size: 125 1500 248 2683
Fragments arranged by size:
2683 1500 248 125
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
SmaI CCC'GGG
Cuts at: 1578 1578
Size: 4556
SmlI C'TyrA_G
Cuts at: 226 1711 2626 3118 3380 226
Size: 1485 915 492 262 1402
Fragments arranged by size:
1485 1402 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4556
SphI G_CATG'C
Cuts at: 1138 1609 2109 1138
Size: 471 500 3585
Fragments arranged by size:
3585 500 471
StyI C'CwwG_G
Cuts at: 107 1061 1256 1690 2044 107
Size: 954 195 434 354 2619
Fragments arranged by size:
2619 954 434 354 195
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 426 2139 2272 2731 2940 426
Size: 1713 133 459 209 2042
Fragments arranged by size:
2042 1713 459 209 133
TatI w'GTAC_w
Cuts at: 542 1095 1451 4071 542
Size: 553 356 2620 1027
Fragments arranged by size:
2620 1027 553 356
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1252 1284 4073 4105 1252
Size: 32 2789 32 1703
Fragments arranged by size:
2789 1703 32 32
Tth111I GACn'n_nGTC
Cuts at: 745 2353 745
Size: 1608 2948
XbaI T'CTAG_A
Cuts at: 1504 1587 1504
Size: 83 4473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 704 2051 704
Size: 1347 3209
XhoI C'TCGA_G
Cuts at: 226 226
Size: 4556
XmaI C'CCGG_G
Cuts at: 1576 1576
Size: 4556
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AleI AloI AscI AvrII BaeI BcgI
BmtI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FspAI HpaI MfeI
NdeI NheI NotI NruI PacI PfoI PmlI PpuMI
PsiI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI