Restriction map for pF1KB5834
 (Linear) (Six-base) MAPSORT of: KIBB5834.seq  Check: 8269  from: 1  to: 1443



KIBB5834.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     302    1443 
   Size:        302    1141
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1066    1443 
   Size:       1066     377
 
AfeI AGC'GCT

Cuts at:      0      46     286     499    1443 
   Size:         46     240     213     944

  Fragments arranged by size: 

                 944     240     213      46
 
AhdI GACnn_n'nnGTC

Cuts at:      0     312     963    1443 
   Size:        312     651     480

  Fragments arranged by size: 

                 651     480     312
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1179    1443 
   Size:       1179     264
 
AlwNI CAG_nnn'CTG

Cuts at:      0     339     582     682    1044    1443 
   Size:        339     243     100     362     399

  Fragments arranged by size: 

                 399     362     339     243     100
 
ApaI G_GGCC'C

Cuts at:      0     570    1282    1443 
   Size:        570     712     161

  Fragments arranged by size: 

                 712     570     161
 
AvaI C'yCGr_G

Cuts at:      0     118    1139    1172    1443 
   Size:        118    1021      33     271

  Fragments arranged by size: 

                1021     271     118      33
 
BamHI G'GATC_C

Cuts at:      0     454    1074    1443 
   Size:        454     620     369

  Fragments arranged by size: 

                 620     454     369
 
BanI G'GyrC_C

Cuts at:      0     253     378    1163    1443 
   Size:        253     125     785     280

  Fragments arranged by size: 

                 785     280     253     125
 
BanII G_rGCy'C

Cuts at:      0     148     169     251     570     863     896    1148    1199 
   Size:        148      21      82     319     293      33     252      51

Cuts at:   1199    1282    1394    1438    1443 
   Size:         83     112      44       5

  Fragments arranged by size: 

                 319     293     252     148     112      83      82      51
                  44      33      21       5
 
BbeI G_GCGC'C

Cuts at:      0     257     382    1443 
   Size:        257     125    1061

  Fragments arranged by size: 

                1061     257     125
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      76    1443 
   Size:         76    1367
 
BbvCI CC'TCA_GC

Cuts at:      0     526    1443 
   Size:        526     917
 
BfrBI ATG'CAT

Cuts at:      0    1156    1443 
   Size:       1156     287
 
BglI GCCn_nnn'nGGC

Cuts at:      0     512     677    1443 
   Size:        512     165     766

  Fragments arranged by size: 

                 766     512     165
 
BlpI GC'TnA_GC

Cuts at:      0     281     890    1443 
   Size:        281     609     553

  Fragments arranged by size: 

                 609     553     281
 
Bme1580I G_kGCm'C

Cuts at:      0     570     785    1282    1443 
   Size:        570     215     497     161

  Fragments arranged by size: 

                 570     497     215     161
 
BmgBI CAC'GTC

Cuts at:      0     965    1443 
   Size:        965     478
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1102    1427    1443 
   Size:       1102     325      16

  Fragments arranged by size: 

                1102     325      16
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     442     604    1443 
   Size:        433       9     162     839

  Fragments arranged by size: 

                 839     433     162       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     245     398     526     971    1443 
   Size:        245     153     128     445     472

  Fragments arranged by size: 

                 472     445     245     153     128
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     229    1443 
   Size:        229    1214
 
BsaAI yAC'GTr

Cuts at:      0     522    1443 
   Size:        522     921
 
BsaHI Gr'CG_yC

Cuts at:      0     254     379    1443 
   Size:        254     125    1064

  Fragments arranged by size: 

                1064     254     125
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     971    1001    1443 
   Size:        971      30     442

  Fragments arranged by size: 

                 971     442      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     853    1198    1443 
   Size:        853     345     245

  Fragments arranged by size: 

                 853     345     245
 
BseYI C'CCAG_C

Cuts at:      0     876    1281    1379    1443 
   Size:        876     405      98      64

  Fragments arranged by size: 

                 876     405      98      64
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     352     496    1443 
   Size:        341      11     144     947

  Fragments arranged by size: 

                 947     341     144      11
 
BsiEI CG_ry'CG

Cuts at:      0     305     663    1443 
   Size:        305     358     780

  Fragments arranged by size: 

                 780     358     305
 
BsiHKAI G_wGCw'C

Cuts at:      0      86     148     169     863    1199    1249    1443 
   Size:         86      62      21     694     336      50     194

  Fragments arranged by size: 

                 694     336     194      86      62      50      21
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     148     169     251     570     785     863     896 
   Size:         86      62      21      82     319     215      78      33

Cuts at:    896    1148    1199    1249    1282    1394    1438    1443 
   Size:        252      51      50      33     112      44       5

  Fragments arranged by size: 

                 319     252     215     112      86      82      78      62
                  51      50      44      33      33      21       5
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     302    1443 
   Size:        302    1141
 
BsrBI CCG'CTC

Cuts at:      0    1364    1443 
   Size:       1364      79
 
BsrDI GCAATG_nn'

Cuts at:      0     911    1443 
   Size:        911     532
 
BsrFI r'CCGG_y

Cuts at:      0     658     749    1443 
   Size:        658      91     694

  Fragments arranged by size: 

                 694     658      91
 
BsrGI T'GTAC_A

Cuts at:      0     434    1343    1443 
   Size:        434     909     100

  Fragments arranged by size: 

                 909     434     100
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1041    1443 
   Size:       1041     402
 
BstYI r'GATC_y

Cuts at:      0     454    1074    1443 
   Size:        454     620     369

  Fragments arranged by size: 

                 620     454     369
 
Bsu36I CC'TnA_GG

Cuts at:      0     675    1094    1371    1443 
   Size:        675     419     277      72

  Fragments arranged by size: 

                 675     419     277      72
 
BtgI C'CryG_G

Cuts at:      0     102     230     560    1443 
   Size:        102     128     330     883

  Fragments arranged by size: 

                 883     330     128     102
 
BtsI GCAGTG_nn'

Cuts at:      0      17     257     275    1320    1443 
   Size:         17     240      18    1045     123

  Fragments arranged by size: 

                1045     240     123      18      17
 
EaeI y'GGCC_r

Cuts at:      0      92     156     302     627     660    1443 
   Size:         92      64     146     325      33     783

  Fragments arranged by size: 

                 783     325     146      92      64      33
 
EagI C'GGCC_G

Cuts at:      0     302     660    1443 
   Size:        302     358     783

  Fragments arranged by size: 

                 783     358     302
 
EarI CTCTTCn'nnn_

Cuts at:      0    1354    1443 
   Size:       1354      89
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     256    1443 
   Size:        256    1187
 
EcoICRI GAG'CTC

Cuts at:      0     146     167     861    1197    1443 
   Size:        146      21     694     336     246

  Fragments arranged by size: 

                 694     336     246     146      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     442     604    1066    1443 
   Size:        433       9     162     462     377

  Fragments arranged by size: 

                 462     433     377     162       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     566     679    1098    1122    1278    1367    1443 
   Size:        566     113     419      24     156      89      76

  Fragments arranged by size: 

                 566     419     156     113      89      76      24
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      43    1443 
   Size:         43    1400
 
FseI GG_CCGG'CC

Cuts at:      0     662    1443 
   Size:        662     781
 
FspI TGC'GCA

Cuts at:      0     910    1031    1443 
   Size:        910     121     412

  Fragments arranged by size: 

                 910     412     121
 
HaeII r_GCGC'y

Cuts at:      0      48     257     288     382     501    1443 
   Size:         48     209      31      94     119     942

  Fragments arranged by size: 

                 942     209     119      94      48      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     298     330     949     981    1443 
   Size:        298      32     619      32     462

  Fragments arranged by size: 

                 619     462     298      32      32
 
HindIII A'AGCT_T

Cuts at:      0     400    1443 
   Size:        400    1043
 
KasI G'GCGC_C

Cuts at:      0     253     378    1443 
   Size:        253     125    1065

  Fragments arranged by size: 

                1065     253     125
 
MslI CAynn'nnrTG

Cuts at:      0    1161    1443 
   Size:       1161     282
 
MspA1I CmG'CkG

Cuts at:      0     368     668    1443 
   Size:        368     300     775

  Fragments arranged by size: 

                 775     368     300
 
NaeI GCC'GGC

Cuts at:      0     660    1443 
   Size:        660     783
 
NarI GG'CG_CC

Cuts at:      0     254     379    1443 
   Size:        254     125    1064

  Fragments arranged by size: 

                1064     254     125
 
NgoMIV G'CCGG_C

Cuts at:      0     658    1443 
   Size:        658     785
 
NsiI A_TGCA'T

Cuts at:      0    1158    1443 
   Size:       1158     285
 
NspI r_CATG'y

Cuts at:      0    1030    1443 
   Size:       1030     413
 
PasI CC'CwG_GG

Cuts at:      0     762    1119    1275    1443 
   Size:        762     357     156     168

  Fragments arranged by size: 

                 762     357     168     156
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      62    1443 
   Size:         62    1381
 
PshAI GACnn'nnGTC

Cuts at:      0    1420    1443 
   Size:       1420      23
 
PspOMI G'GGCC_C

Cuts at:      0     566    1278    1443 
   Size:        566     712     165

  Fragments arranged by size: 

                 712     566     165
 
PspXI vC'TCGA_Gb

Cuts at:      0     118    1443 
   Size:        118    1325
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     418     450    1443 
   Size:        418      32     993

  Fragments arranged by size: 

                 993     418      32
 
PvuII CAG'CTG

Cuts at:      0     368    1443 
   Size:        368    1075
 
SacI G_AGCT'C

Cuts at:      0     148     169     863    1199    1443 
   Size:        148      21     694     336     244

  Fragments arranged by size: 

                 694     336     244     148      21
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1354    1443 
   Size:       1354      89
 
SexAI A'CCwGG_T

Cuts at:      0    1296    1443 
   Size:       1296     147
 
SfoI GGC'GCC

Cuts at:      0     255     380    1443 
   Size:        255     125    1063

  Fragments arranged by size: 

                1063     255     125
 
SmlI C'TyrA_G

Cuts at:      0     118    1443 
   Size:        118    1325
 
SphI G_CATG'C

Cuts at:      0    1030    1443 
   Size:       1030     413
 
StyI C'CwwG_G

Cuts at:      0     953    1148    1443 
   Size:        953     195     295

  Fragments arranged by size: 

                 953     295     195
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     318    1443 
   Size:        318    1125
 
TatI w'GTAC_w

Cuts at:      0     434     987    1343    1443 
   Size:        434     553     356     100

  Fragments arranged by size: 

                 553     434     356     100
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1144    1176    1443 
   Size:       1144      32     267

  Fragments arranged by size: 

                1144     267      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     637    1443 
   Size:        637     806
 
XbaI T'CTAG_A

Cuts at:      0    1396    1443 
   Size:       1396      47
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     596    1443 
   Size:        596     847
 
XhoI C'TCGA_G

Cuts at:      0     118    1443 
   Size:        118    1325
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AhdI     AlfI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I     FalI     FseI     FspI    HaeII    Hin4I  HindIII     KasI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI     NspI     PasI
    PflMI    PshAI   PspOMI    PspXI     PsrI    PvuII     SacI     SapI
    SexAI     SfoI     SmlI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AleI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BcgI    BciVI     BclI    BglII     BmtI     BplI    BpuEI    BsaBI
    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BspHI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI    EcoNI    EcoRI    EcoRV    FspAI   HincII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     NcoI     NdeI     NheI
     NotI     NruI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI     PsiI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI     XmaI     XmnI     ZraI
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