Restriction map for pF1KB9897
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BcnI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRII      EcoRV       EcoT22I     FauI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MluI        MluNI       MroI        MscI        
MspA1I      MspI        MvaI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScrFI       
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          768  GTATAC               GT^[AC][GT]AC
AflII        1120  CTTAAG               C^TTAAG
AhdI          523  GACTACCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          210  AGCT                 AG^CT
AocI         1444  CCTGAGG              CC^T[ACGT]AGG
AseI         1104  ATTAAT               AT^TAAT
AsnI         1104  ATTAAT               AT^TAAT
AspEI         523  GACTACCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          729  GGCACC               G^G[CT][GA]CC
BanII        1242  GAGCCC               G[GA]GC[CT]^C
BbvI          199  GCAGC                GCAGC(8/12)
BclI          822  TGATCA               T^GATCA
BfaI         1033  CTAG                 C^TAG
BfrI         1120  CTTAAG               C^TTAAG
BmyI         1242  GAGCCC               G[AGT]GC[ACT]^C
BsaAI         552  TACGTG               [CT]AC^GT[GA]
BsiYI          91  CCTAATTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           91  CCTAATTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I     1242  GAGCCC               G[AGT]GC[ACT]^C
BspLU11I     1525  ACATGT               A^CATGT
BspMI          19  GCAGGT               ACCTGC(4/8)
BsrI          884  CCAGT                ACTGG(1/-1)
Bst1107I      768  GTATAC               GTA^TAC
BstAPI        187  GCAAAAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I       768  GTATAC               GTA^TAC
Bsu36I       1444  CCTGAGG              CC^T[ACGT]AGG
DsaI          290  CCATGG               C^C[GA][CT]GG
EaeI          467  TGGCCG               [CT]^GGCC[GA]
Eam1105I      523  GACTACCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1444  CCTGAGG              CC^T[ACGT]AGG
EcoNI          91  CCTAATTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          822  TGATCA               T^GATCA
MaeI         1033  CTAG                 C^TAG
MaeII         553  ACGT                 A^CGT
MfeI          463  CAATTG               C^AATTG
MunI          463  CAATTG               C^AATTG
NcoI          290  CCATGG               C^CATGG
NspI         1525  ACATGT               [GA]CATG^[CT]
PleI          559  GAGTC                GAGTC(4/5)
PshBI        1104  ATTAAT               AT^TAAT
ScaI           69  AGTACT               AGT^ACT
SfcI         1420  CTACAG               C^T[GA][CT]AG
SspI          832  AATATT               AAT^ATT
StuI          306  AGGCCT               AGG^CCT
TaqI         1452  TCGA                 T^CGA
TseI          199  GCAGC                G^C[AT]GC
TthHB8I      1452  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI           70  GTAC                 GT^AC
              434  GTAC                
AflIII        553  ACGTGT               A^C[GA][CT]GT
             1525  ACATGT              
Asp700I       385  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1315  GAACTTCTTC          
BglII         721  AGATCT               A^GATCT
             1116  AGATCT              
BsaBI         868  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              910  GATTATGATC          
BsaI          880  GAGACC               GGTCTC(1/5)
              691  GGTCTC              
BsaJI         290  CCATGG               C^C[ACGT][ACGT]GG
              995  CCTTGG              
BstYI         721  AGATCT               [GA]^GATC[CT]
             1116  AGATCT              
EcoRI         270  GAATTC               G^AATTC
              976  GAATTC              
EcoT14I       290  CCATGG               C^C[AT][AT]GG
              995  CCTTGG              
MamI          868  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              910  GATTATGATC          
MflI          721  AGATCT               [GA]^GATC[CT]
             1116  AGATCT              
NlaIV         729  GGCACC               GG[ACGT]^[ACGT]CC
              991  GGGACC              
RsaI           70  GTAC                 GT^AC
              434  GTAC                
SfaNI         364  GCATC                GCATC(5/9)
             1379  GCATC               
SmlI          898  CTTGAG               C^T[CT][GA]AG
             1120  CTTAAG              
StyI          290  CCATGG               C^C[AT][AT]GG
              995  CCTTGG              
TfiI          601  GAATC                G^A[AT]TC
              892  GATTC               
XhoII         721  AGATCT               [GA]^GATC[CT]
             1116  AGATCT              
XmnI          385  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1315  GAACTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AcsI        AlwI        ApoI        AvaII       
BsmAI       BsmFI       BstF5I      Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MseI        MslI        MwoI        NdeII       NlaIII      
PpuMI       Sau3AI      Sau96I      Tru9I       Tsp45I      
Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com