Restriction map for pF1KE9442
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspI        AviII       BcgI        BciVI       BclI        
BfrI        BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DraIII      DrdI        
Eco47III    EcoO65I     EcoRI       EcoRV       FbaI        
FseI        FspI        HincII      HindII      HpaI        
KpnI        MfeI        MseI        MunI        NdeI        
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SexAI       SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        Tru9I       Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII        758  TCCGGA               T^CCGGA
AhdI         1872  GACCTTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1872  GACCTTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        2070  CCTAGG               C^CTAGG
BalI          752  TGGCCA               TGG^CCA
BamHI        1406  GGATCC               G^GATCC
BglII         966  AGATCT               A^GATCT
BlnI         2070  CCTAGG               C^CTAGG
BlpI         1188  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1188  GCTGAGC              GC^T[ACGT]AGC
BsaBI        2623  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         758  TCCGGA               T^CCGGA
BsiEI        2564  CGGCCG               CG[GA][CT]^CG
BspEI         758  TCCGGA               T^CCGGA
BsrDI        1733  GCAATG               GCAATG(2/0)
BstAPI        420  GCAGTCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1188  GCTGAGC              GC^T[ACGT]AGC
EagI         2564  CGGCCG               C^GGCCG
Eam1105I     1872  GACCTTTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2564  CGGCCG               C^GGCCG
Eco52I       2564  CGGCCG               C^GGCCG
EcoNI        1639  CCTACTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1336  ATGCAT               ATGCA^T
HindIII      2642  AAGCTT               A^AGCTT
KspI         2275  CCGCGG               CCGC^GG
MamI         2623  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          611  ACGCGT               A^CGCGT
MluNI         752  TGGCCA               TGG^CCA
MroI          758  TCCGGA               T^CCGGA
MscI          752  TGGCCA               TGG^CCA
NaeI           64  GCCGGC               GCC^GGC
NgoMIV         64  GCCGGC               G^CCGGC
NsiI         1336  ATGCAT               ATGCA^T
SacII        2275  CCGCGG               CCGC^GG
SbfI          561  CCTGCAGG             CCTGCA^GG
ScaI         1947  AGTACT               AGT^ACT
SfiI         1698  GGCCGTGTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI           87  CCCGGG               CCC^GGG
Sse8387I      561  CCTGCAGG             CCTGCA^GG
XmaCI          87  CCCGGG               C^CCGGG
XmaI           87  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI           70  GGCGCC               G[GA]^CG[CT]C
             1816  GGCGCC              
AflIII        611  ACGCGT               A^C[GA][CT]GT
             2895  ACACGT              
AocI         1243  CCTGAGG              CC^T[ACGT]AGG
             2167  CCTGAGG             
Asp700I      1492  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2217  GAACTCCTTC          
BbeI           70  GGCGCC               GGCGC^C
             1816  GGCGCC              
BbrPI        2320  CACGTG               CAC^GTG
             2896  CACGTG              
BbsI         1492  GAAGAC               GAAGAC(2/6)
             1518  GTCTTC              
BpuAI        1492  GAAGAC               GAAGAC(2/6)
             1518  GTCTTC              
BsaAI        2320  CACGTG               [CT]AC^GT[GA]
             2896  CACGTG              
BsaHI          70  GGCGCC               G[GA]^CG[CT]C
             1816  GGCGCC              
BsaI         2674  GAGACC               GGTCTC(1/5)
              334  GGTCTC              
BsaWI         758  TCCGGA               [AT]^CCGG[AT]
             1229  TCCGGT              
BsmI          325  GCATTC               GAATGC(1/-1)
              898  GCATTC              
BsrFI          64  GCCGGC               [GA]^CCGG[CT]
             2242  GCCGGT              
Bsu36I       1243  CCTGAGG              CC^T[ACGT]AGG
             2167  CCTGAGG             
Cfr10I         64  GCCGGC               [GA]^CCGG[CT]
             2242  GCCGGT              
Eco81I       1243  CCTGAGG              CC^T[ACGT]AGG
             2167  CCTGAGG             
HgaI          285  GCGTC                GACGC(5/10)
             2003  GCGTC               
Hin1I          70  GGCGCC               G[GA]^CG[CT]C
             1816  GGCGCC              
KasI           70  GGCGCC               G^GCGCC
             1816  GGCGCC              
MaeII        2321  ACGT                 A^CGT
             2897  ACGT                
NarI           70  GGCGCC               GG^CGCC
             1816  GGCGCC              
PmaCI        2320  CACGTG               CAC^GTG
             2896  CACGTG              
PmlI         2320  CACGTG               CAC^GTG
             2896  CACGTG              
SapI         2580  GAAGAGC              GCTCTTC(1/4)
             2484  GCTCTTC             
SfoI           70  GGCGCC               GGC^GCC
             1816  GGCGCC              
XmnI         1492  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2217  GAACTCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbvCI       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        StyI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       
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