Restriction map for pF1KE5338
 (Linear) (Six-base) MAPSORT of: KIEE5338.seq  Check: 2693  from: 1  to: 792



KIEE5338.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AatII G_ACGT'C

Cuts at:      0     443     792 
   Size:        443     349
 
Acc65I G'GTAC_C

Cuts at:      0     355     792 
   Size:        355     437
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     529     792 
   Size:        529     263
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     370     402     792 
   Size:        370      32     390

  Fragments arranged by size: 

                 390     370      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     319     792 
   Size:        319     473
 
AvaI C'yCGr_G

Cuts at:      0     623     792 
   Size:        623     169
 
BanI G'GyrC_C

Cuts at:      0      10     355     492     792 
   Size:         10     345     137     300

  Fragments arranged by size: 

                 345     300     137      10
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     621     792 
   Size:        621     171
 
Bme1580I G_kGCm'C

Cuts at:      0      13     164     686     792 
   Size:         13     151     522     106

  Fragments arranged by size: 

                 522     151     106      13
 
BmgBI CAC'GTC

Cuts at:      0     375     792 
   Size:        375     417
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       9     471     792 
   Size:          9     462     321

  Fragments arranged by size: 

                 462     321       9
 
BsaHI Gr'CG_yC

Cuts at:      0     440     792 
   Size:        440     352
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       3      33     370     400     792 
   Size:          3      30     337      30     392

  Fragments arranged by size: 

                 392     337      30      30       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31     792 
   Size:         31     761
 
BseYI C'CCAG_C

Cuts at:      0     163     208     487     500     762     792 
   Size:        163      45     279      13     262      30

  Fragments arranged by size: 

                 279     262     163      45      30      13
 
BsiEI CG_ry'CG

Cuts at:      0     702     792 
   Size:        702      90
 
BsmI GAATG_Cn'

Cuts at:      0     186     792 
   Size:        186     606
 
Bsp1286I G_dGCh'C

Cuts at:      0      13     164     686     792 
   Size:         13     151     522     106

  Fragments arranged by size: 

                 522     151     106      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     718     792 
   Size:        718      74
 
BsrDI GCAATG_nn'

Cuts at:      0     560     792 
   Size:        560     232
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     670     792 
   Size:        670     122
 
BstYI r'GATC_y

Cuts at:      0     204     316     792 
   Size:        204     112     476

  Fragments arranged by size: 

                 476     204     112
 
BtgI C'CryG_G

Cuts at:      0     669     792 
   Size:        669     123
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     492     792 
   Size:        492     300
 
BtsI GCAGTG_nn'

Cuts at:      0     773     792 
   Size:        773      19
 
DraIII CAC_nnn'GTG

Cuts at:      0     375     792 
   Size:        375     417
 
EaeI y'GGCC_r

Cuts at:      0     545     792 
   Size:        545     247
 
HaeII r_GCGC'y

Cuts at:      0     678     792 
   Size:        678     114
 
KpnI G_GTAC'C

Cuts at:      0     359     792 
   Size:        359     433
 
MfeI C'AATT_G

Cuts at:      0     331     792 
   Size:        331     461
 
MscI TGG'CCA

Cuts at:      0     547     792 
   Size:        547     245
 
MslI CAynn'nnrTG

Cuts at:      0     668     792 
   Size:        668     124
 
MspA1I CmG'CkG

Cuts at:      0     606     792 
   Size:        606     186
 
NcoI C'CATG_G

Cuts at:      0     669     792 
   Size:        669     123
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     375     792 
   Size:        375     417
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     370     402     792 
   Size:        370      32     390

  Fragments arranged by size: 

                 390     370      32
 
PsiI TTA'TAA

Cuts at:      0     269     792 
   Size:        269     523
 
PstI C_TGCA'G

Cuts at:      0     236     729     792 
   Size:        236     493      63

  Fragments arranged by size: 

                 493     236      63
 
PvuI CG_AT'CG

Cuts at:      0     702     792 
   Size:        702      90
 
PvuII CAG'CTG

Cuts at:      0     606     792 
   Size:        606     186
 
SfcI C'TryA_G

Cuts at:      0     232     725     792 
   Size:        232     493      67

  Fragments arranged by size: 

                 493     232      67
 
SmaI CCC'GGG

Cuts at:      0     625     792 
   Size:        625     167
 
SspI AAT'ATT

Cuts at:      0     300     792 
   Size:        300     492
 
StyI C'CwwG_G

Cuts at:      0     566     669     792 
   Size:        566     103     123

  Fragments arranged by size: 

                 566     123     103
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      28     682     792 
   Size:         28     654     110

  Fragments arranged by size: 

                 654     110      28
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       3      35     308     340     792 
   Size:          3      32     273      32     452

  Fragments arranged by size: 

                 452     273      32      32       3
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     484     792 
   Size:        484     308
 
XmaI C'CCGG_G

Cuts at:      0     623     792 
   Size:        623     169
 
ZraI GAC'GTC

Cuts at:      0     441     792 
   Size:        441     351
 

 Enzymes that do cut:

    AatII   Acc65I     AlfI     AloI    AlwNI     AvaI     BanI    BciVI
 Bme1580I    BmgBI     BmrI    BsaHI    BsaXI    BseRI    BseYI    BsiEI
     BsmI Bsp1286I    BspMI    BsrDI    BstXI    BstYI     BtgI    BtgZI
     BtsI   DraIII     EaeI    HaeII     KpnI     MfeI     MscI     MslI
   MspA1I     NcoI    PflMI     PpiI     PsiI     PstI     PvuI    PvuII
     SfcI     SmaI     SspI     StyI    TaqII     TstI     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI     BsgI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI
     DraI     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     MluI     MmeI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI    PpuMI    PshAI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI  Tth111I
     XbaI     XhoI     XmnI
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