Restriction map for pF1KE1746
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BcgI        BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EaeI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT14I     FbaI        
FokI        FseI        FspI        HaeII       HapII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII          18  CGCG                 CG^CG
AflIII        682  ACATGT               A^C[GA][CT]GT
AhdI          250  GACACTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI           26  GGGCCC               GGGCC^C
AspEI         250  GACACTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII          26  GGGCCC               G[GA]GC[CT]^C
BbvCI          39  CCTCAGC              CCTCAGC(-5/-2)
BciVI         614  GTATCC               GTATCC(6/5)
BmyI           26  GGGCCC               G[AGT]GC[ACT]^C
BsgI           58  CTGCAC               GTGCAG(16/14)
BsmAI         366  GTCTC                GTCTC(1/5)
Bsp1286I       26  GGGCCC               G[AGT]GC[ACT]^C
BspLU11I      682  ACATGT               A^CATGT
BssKI          30  CCTGG                ^CC[ACGT]GG
BstNI          30  CCTGG                CC^[AT]GG
BstUI          18  CGCG                 CG^CG
DpnII         538  GATC                 ^GATC
Eam1105I      250  GACACTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          368  CTCTTC               CTCTTC(1/4)
EcoRI         208  GAATTC               G^AATTC
EcoRII         30  CCTGG                ^CC[AT]GG
EcoT22I       423  ATGCAT               ATGCA^T
HaeIII         27  GGCC                 GG^CC
HgaI           16  GACGC                GACGC(5/10)
Ksp632I       368  CTCTTC               CTCTTC(1/4)
MaeII         501  ACGT                 A^CGT
MboI          538  GATC                 ^GATC
MslI          640  CACAGATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           30  CCTGG                CC^[AT]GG
MvnI           18  CGCG                 CG^CG
NdeII         538  GATC                 ^GATC
NsiI          423  ATGCAT               ATGCA^T
NspI          682  ACATGT               [GA]CATG^[CT]
PleI          364  GAGTC                GAGTC(4/5)
PvuII          78  CAGCTG               CAG^CTG
Sau3AI        538  GATC                 ^GATC
ScrFI          30  CCTGG                CC^[ACGT]GG
SmlI          361  CTTGAG               C^T[CT][GA]AG
SspI          325  AATATT               AAT^ATT
TaqI          453  TCGA                 T^CGA
TfiI          316  GAATC                G^A[AT]TC
Tsp45I        719  GTGAC                ^GT[GC]AC
TthHB8I       453  TCGA                 T^CGA
XcmI           90  CCAACAAATATATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI          201  AAATTC               [GA]^AATT[CT]
              208  GAATTC              
AluI           79  AGCT                 AG^CT
              216  AGCT                
ApoI          201  AAATTC               [GA]^AATT[CT]
              208  GAATTC              
AvaII          36  GGTCC                G^G[AT]CC
              159  GGTCC               
BbvI          623  GCAGC                GCAGC(8/12)
               57  GCTGC               
BsaJI          29  CCCTGG               C^C[ACGT][ACGT]GG
               30  CCTGGG              
BsiYI          39  CCTCAGCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              619  CCAAGCAGCGG         
BslI           39  CCTCAGCGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              619  CCAAGCAGCGG         
BsrDI         140  GCAATG               GCAATG(2/0)
              492  GCAATG              
BsrI          195  ACTGG                ACTGG(1/-1)
              512  CCAGT               
Cac8I          24  GCGGGC               GC[ACGT]^[ACGT]GC
               53  GCCTGC              
DdeI           40  CTCAG                C^T[ACGT]AG
              565  CTTAG               
DraI          150  TTTAAA               TTT^AAA
              229  TTTAAA              
FauI           19  GCGGG                CCCGC(4/6)
               24  GCGGG               
Fnu4HI         57  GCTGC                GC^[ACGT]GC
              623  GCAGC               
HinfI         316  GAATC                G^A[ACGT]TC
              364  GAGTC               
ItaI           57  GCTGC                GC^[ACGT]GC
              623  GCAGC               
MspA1I         78  CAGCTG               C[AC]G^C[GT]G
              624  CAGCGG              
MwoI           19  GCGGGGCGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               44  GCGTGGTCTGC         
NlaIV          26  GGGCCC               GG[ACGT]^[ACGT]CC
               35  GGGTCC              
PpuMI          35  GGGTCCT              [GA]G^G[AT]CC[CT]
              158  AGGTCCT             
TseI           57  GCTGC                G^C[AT]GC
              623  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AciI        AfaI        Cfr13I      DraII       EcoO109I    
MaeIII      MboII       MnlI        MseI        NlaIII      
RsaI        Sau96I      Tru9I       Tsp509I     TspRI       
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