Restriction map for pF1KE3328
 (Linear) (Six-base) MAPSORT of: KIEE3328.seq  Check: 8389  from: 1  to: 2808



KIEE3328.orf

 With 182 enzymes: SgfI * 

                        March 2, 2011 13:37  ..

AclI AA'CG_TT

Cuts at:      0    1773    2808 
   Size:       1773    1035
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     450    1197    1525    1660    2232    2441    2462    2808 
   Size:        450     747     328     135     572     209      21     346

  Fragments arranged by size: 

                 747     572     450     346     328     209     135      21
            
 
AflIII A'CryG_T

Cuts at:      0    1453    2808 
   Size:       1453    1355
 
AlwNI CAG_nnn'CTG

Cuts at:      0     133    2148    2317    2808 
   Size:        133    2015     169     491

  Fragments arranged by size: 

                2015     491     169     133
 
ApaLI G'TGCA_C

Cuts at:      0    2217    2808 
   Size:       2217     591
 
ApoI r'AATT_y

Cuts at:      0     103     312     865    1147    1270    2273    2288    2434 
   Size:        103     209     553     282     123    1003      15     146

Cuts at:   2434    2808 
   Size:        374

  Fragments arranged by size: 

                1003     553     374     282     209     146     123     103
                  15
 
AseI AT'TA_AT

Cuts at:      0     331    1993    2808 
   Size:        331    1662     815

  Fragments arranged by size: 

                1662     815     331
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2389    2422    2808 
   Size:       2389      33     386

  Fragments arranged by size: 

                2389     386      33
 
BamHI G'GATC_C

Cuts at:      0     228    2808 
   Size:        228    2580
 
BanII G_rGCy'C

Cuts at:      0     578    2758    2808 
   Size:        578    2180      50

  Fragments arranged by size: 

                2180     578      50
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1010    2162    2238    2433    2698    2808 
   Size:       1010    1152      76     195     265     110

  Fragments arranged by size: 

                1152    1010     265     195     110      76
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2203    2237    2808 
   Size:       2203      34     571

  Fragments arranged by size: 

                2203     571      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     688    2751    2808 
   Size:        688    2063      57

  Fragments arranged by size: 

                2063     688      57
 
BclI T'GATC_A

Cuts at:      0     650    1281    2808 
   Size:        650     631    1527

  Fragments arranged by size: 

                1527     650     631
 
BfrBI ATG'CAT

Cuts at:      0    2763    2808 
   Size:       2763      45
 
Bme1580I G_kGCm'C

Cuts at:      0     276    1325    2221    2808 
   Size:        276    1049     896     587

  Fragments arranged by size: 

                1049     896     587     276
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257    1580    2808 
   Size:        257    1323    1228

  Fragments arranged by size: 

                1323    1228     257
 
Bpu10I CC'TnA_GC

Cuts at:      0    1713    2808 
   Size:       1713    1095
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     977    2290    2657    2808 
   Size:        977    1313     367     151

  Fragments arranged by size: 

                1313     977     367     151
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1512    2522    2808 
   Size:       1512    1010     286

  Fragments arranged by size: 

                1512    1010     286
 
BsaAI yAC'GTr

Cuts at:      0      97    2808 
   Size:         97    2711
 
BsaBI GATnn'nnATC

Cuts at:      0    1400    2808 
   Size:       1400    1408
 
BsaWI w'CCGG_w

Cuts at:      0      21    2808 
   Size:         21    2787
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     764     794    2808 
   Size:        764      30    2014

  Fragments arranged by size: 

                2014     764      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1577    1630    2704    2808 
   Size:       1577      53    1074     104

  Fragments arranged by size: 

                1577    1074     104      53
 
BseYI C'CCAG_C

Cuts at:      0      47    1420    1897    2808 
   Size:         47    1373     477     911

  Fragments arranged by size: 

                1373     911     477      47
 
BsiHKAI G_wGCw'C

Cuts at:      0    2221    2758    2808 
   Size:       2221     537      50

  Fragments arranged by size: 

                2221     537      50
 
BsmI GAATG_Cn'

Cuts at:      0     993    2808 
   Size:        993    1815
 
Bsp1286I G_dGCh'C

Cuts at:      0     276     578    1325    2221    2758    2808 
   Size:        276     302     747     896     537      50

  Fragments arranged by size: 

                 896     747     537     302     276      50
 
BspEI T'CCGG_A

Cuts at:      0      21    2808 
   Size:         21    2787
 
BspHI T'CATG_A

Cuts at:      0    1956    2450    2808 
   Size:       1956     494     358

  Fragments arranged by size: 

                1956     494     358
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     413     678    1415    2808 
   Size:        413     265     737    1393

  Fragments arranged by size: 

                1393     737     413     265
 
BsrDI GCAATG_nn'

Cuts at:      0     811    2808 
   Size:        811    1997
 
BstBI TT'CG_AA

Cuts at:      0     555    2603    2808 
   Size:        555    2048     205

  Fragments arranged by size: 

                2048     555     205
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2022    2656    2808 
   Size:       2022     634     152

  Fragments arranged by size: 

                2022     634     152
 
BstYI r'GATC_y

Cuts at:      0     228    2808 
   Size:        228    2580
 
Bsu36I CC'TnA_GG

Cuts at:      0    2201    2808 
   Size:       2201     607
 
BtgI C'CryG_G

Cuts at:      0    1350    2808 
   Size:       1350    1458
 
BtsI GCAGTG_nn'

Cuts at:      0     707    2221    2517    2808 
   Size:        707    1514     296     291

  Fragments arranged by size: 

                1514     707     296     291
 
DraI TTT'AAA

Cuts at:      0     482    2808 
   Size:        482    2326
 
DraIII CAC_nnn'GTG

Cuts at:      0     271    2216    2808 
   Size:        271    1945     592

  Fragments arranged by size: 

                1945     592     271
 
EaeI y'GGCC_r

Cuts at:      0     735    2568    2808 
   Size:        735    1833     240

  Fragments arranged by size: 

                1833     735     240
 
EarI CTCTTCn'nnn_

Cuts at:      0    1960    2456    2808 
   Size:       1960     496     352

  Fragments arranged by size: 

                1960     496     352
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     568    2808 
   Size:        568    2240
 
EcoICRI GAG'CTC

Cuts at:      0    2756    2808 
   Size:       2756      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     450    1197    1525    1580    1660    2232    2441 
   Size:        257     193     747     328      55      80     572     209

Cuts at:   2441    2462    2808 
   Size:         21     346

  Fragments arranged by size: 

                 747     572     346     328     257     209     193      80
                  55      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15    2563    2630    2808 
   Size:         15    2548      67     178

  Fragments arranged by size: 

                2548     178      67      15
 
EcoRI G'AATT_C

Cuts at:      0     103     312    2808 
   Size:        103     209    2496

  Fragments arranged by size: 

                2496     209     103
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     642     674     764     796    1969    2001    2473    2505 
   Size:        642      32      90      32    1173      32     472      32

Cuts at:   2505    2808 
   Size:        303

  Fragments arranged by size: 

                1173     642     472     303      90      32      32      32
                  32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     672     879    2170    2808 
   Size:        672     207    1291     638

  Fragments arranged by size: 

                1291     672     638     207
 
MscI TGG'CCA

Cuts at:      0     737    2808 
   Size:        737    2071
 
MslI CAynn'nnrTG

Cuts at:      0     341    2808 
   Size:        341    2467
 
MspA1I CmG'CkG

Cuts at:      0       7     130     168    2314    2808 
   Size:          7     123      38    2146     494

  Fragments arranged by size: 

                2146     494     123      38       7
 
NdeI CA'TA_TG

Cuts at:      0     372    2808 
   Size:        372    2436
 
NsiI A_TGCA'T

Cuts at:      0    2765    2808 
   Size:       2765      43
 
NspI r_CATG'y

Cuts at:      0    1136    1457    2107    2417    2808 
   Size:       1136     321     650     310     391

  Fragments arranged by size: 

                1136     650     391     321     310
 
PciI A'CATG_T

Cuts at:      0    1453    2808 
   Size:       1453    1355
 
PmlI CAC'GTG

Cuts at:      0      97    2808 
   Size:         97    2711
 
PpuMI rG'GwC_Cy

Cuts at:      0      15    2563    2630    2808 
   Size:         15    2548      67     178

  Fragments arranged by size: 

                2548     178      67      15
 
PsiI TTA'TAA

Cuts at:      0    1514    2808 
   Size:       1514    1294
 
PstI C_TGCA'G

Cuts at:      0     410    2808 
   Size:        410    2398
 
PvuII CAG'CTG

Cuts at:      0       7     168    2808 
   Size:          7     161    2640

  Fragments arranged by size: 

                2640     161       7
 
SacI G_AGCT'C

Cuts at:      0    2758    2808 
   Size:       2758      50
 
SanDI GG'GwC_CC

Cuts at:      0      15    2808 
   Size:         15    2793
 
SfcI C'TryA_G

Cuts at:      0     406    2140    2808 
   Size:        406    1734     668

  Fragments arranged by size: 

                1734     668     406
 
SmlI C'TyrA_G

Cuts at:      0     992    2305    2672    2808 
   Size:        992    1313     367     136

  Fragments arranged by size: 

                1313     992     367     136
 
SphI G_CATG'C

Cuts at:      0    1136    2107    2808 
   Size:       1136     971     701

  Fragments arranged by size: 

                1136     971     701
 
SspI AAT'ATT

Cuts at:      0     786     945    1549    2082    2665    2808 
   Size:        786     159     604     533     583     143

  Fragments arranged by size: 

                 786     604     583     533     159     143
 
StyI C'CwwG_G

Cuts at:      0     201    1160    1308    1595    2808 
   Size:        201     959     148     287    1213

  Fragments arranged by size: 

                1213     959     287     201     148
 
SwaI ATTT'AAAT

Cuts at:      0     482    2808 
   Size:        482    2326
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      61    1867    2733    2808 
   Size:         61    1806     866      75

  Fragments arranged by size: 

                1806     866      75      61
 
Tth111I GACn'n_nGTC

Cuts at:      0    1009    1264    2808 
   Size:       1009     255    1544

  Fragments arranged by size: 

                1544    1009     255
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1315    1705    1806    2808 
   Size:       1315     390     101    1002

  Fragments arranged by size: 

                1315    1002     390     101
 

 Enzymes that do cut:

     AclI     AcuI   AflIII    AlwNI    ApaLI     ApoI     AseI     BaeI
    BamHI    BanII     BbsI     BcgI    BciVI     BclI    BfrBI Bme1580I
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BstBI    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI
   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    Hin4I     MmeI     MscI     MslI   MspA1I     NdeI     NsiI     NspI
     PciI     PmlI    PpuMI     PsiI     PstI    PvuII     SacI    SanDI
     SfcI     SmlI     SphI     SspI     StyI     SwaI    TaqII  Tth111I
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     AscI    AsiSI     AvaI    AvrII
     BanI     BbeI    BbvCI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI    BsaHI     BsgI    BsiEI    BsiWI    BsmBI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DrdI     EagI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     PacI     PasI    PflMI     PfoI     PmeI     PpiI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     StuI     TatI     TstI     XbaI     XhoI
     XmaI     XmnI     ZraI
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