(Circular) (Six-base) MAPSORT of: pF1KE1599.seq Check: 7792 from: 1 to: 6608 pF1KE1599 6608 bp With 182 enzymes: SgfI * August 26, 2010 17:36 .. AccI GT'mk_AC Cuts at: 992 999 3646 992 Size: 7 2647 3954 Fragments arranged by size: 3954 2647 7 Acc65I G'GTAC_C Cuts at: 894 3624 894 Size: 2730 3878 AclI AA'CG_TT Cuts at: 2788 6448 2788 Size: 3660 2948 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 626 3332 4433 4865 5612 5880 626 Size: 2706 1101 432 747 268 1354 Fragments arranged by size: 2706 1354 1101 747 432 268 AflIII A'CryG_T Cuts at: 1687 2827 3960 5064 6041 1687 Size: 1140 1133 1104 977 2254 Fragments arranged by size: 2254 1140 1133 1104 977 AgeI A'CCGG_T Cuts at: 4996 4996 Size: 6608 AhdI GACnn_n'nnGTC Cuts at: 2831 2831 Size: 6608 AleI CACnn'nnGTG Cuts at: 2618 2618 Size: 6608 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1356 1356 Size: 6608 AlwNI CAG_nnn'CTG Cuts at: 3913 5480 3913 Size: 1567 5041 ApaLI G'TGCA_C Cuts at: 5378 5378 Size: 6608 ApoI r'AATT_y Cuts at: 121 148 228 282 453 933 1124 1218 1232 Size: 27 80 54 171 480 191 94 14 Cuts at: 1232 2038 2206 2283 3156 3303 3612 5961 6217 Size: 806 168 77 873 147 309 2349 256 Cuts at: 6217 121 Size: 512 Fragments arranged by size: 2349 873 806 512 480 309 256 191 171 168 147 94 80 77 54 27 14 AseI AT'TA_AT Cuts at: 20 2054 2582 2689 20 Size: 2034 528 107 3939 Fragments arranged by size: 3939 2034 528 107 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6608 AvaI C'yCGr_G Cuts at: 1310 3628 1310 Size: 2318 4290 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3005 3038 3005 Size: 33 6575 BamHI G'GATC_C Cuts at: 3633 3633 Size: 6608 BanI G'GyrC_C Cuts at: 894 3624 4038 4286 4321 894 Size: 2730 414 248 35 3181 Fragments arranged by size: 3181 2730 414 248 35 BanII G_rGCy'C Cuts at: 1562 3622 4652 1562 Size: 2060 1030 3518 Fragments arranged by size: 3518 2060 1030 BbeI G_GCGC'C Cuts at: 4042 4290 4042 Size: 248 6360 BbsI GAAGACnn'nnnn_ Cuts at: 1004 3520 1004 Size: 2516 4092 BbvCI CC'TCA_GC Cuts at: 3008 3008 Size: 6608 BciVI GTATCCnnnnn_n' Cuts at: 2375 4506 5267 6608 2375 Size: 2131 761 1341 2375 Fragments arranged by size: 2375 2131 1341 761 BclI T'GATC_A Cuts at: 1263 2502 3074 4128 1263 Size: 1239 572 1054 3743 Fragments arranged by size: 3743 1239 1054 572 BfrBI ATG'CAT Cuts at: 1601 1601 Size: 6608 BglII A'GATC_T Cuts at: 2946 4123 6160 2946 Size: 1177 2037 3394 Fragments arranged by size: 3394 2037 1177 BlpI GC'TnA_GC Cuts at: 3720 3720 Size: 6608 Bme1580I G_kGCm'C Cuts at: 2760 3415 4233 4326 5382 2760 Size: 655 818 93 1056 3986 Fragments arranged by size: 3986 1056 818 655 93 BmrI ACTGGGnnnn_n' Cuts at: 1897 1972 2387 3820 3901 4236 6380 1897 Size: 75 415 1433 81 335 2144 2125 Fragments arranged by size: 2144 2125 1433 415 335 81 75 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1097 4975 1097 Size: 3878 2730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1083 1092 3439 1083 Size: 9 2347 4252 Fragments arranged by size: 4252 2347 9 Bpu10I CC'TnA_GC Cuts at: 3008 3008 Size: 6608 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2579 3784 4663 5155 5453 2579 Size: 1205 879 492 298 3734 Fragments arranged by size: 3734 1205 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 2931 6312 2931 Size: 3381 3227 BsaAI yAC'GTr Cuts at: 3961 3961 Size: 6608 BsaBI GATnn'nnATC Cuts at: 6 1648 3233 4122 6159 6 Size: 1642 1585 889 2037 455 Fragments arranged by size: 2037 1642 1585 889 455 BsaHI Gr'CG_yC Cuts at: 4039 4287 4989 6485 4039 Size: 248 702 1496 4162 Fragments arranged by size: 4162 1496 702 248 BsaWI w'CCGG_w Cuts at: 3797 4318 4996 5270 5417 5828 3797 Size: 521 678 274 147 411 4577 Fragments arranged by size: 4577 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1070 1070 Size: 6608 BseYI C'CCAG_C Cuts at: 3420 4033 4791 5368 5864 3420 Size: 613 758 577 496 4164 Fragments arranged by size: 4164 758 613 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 434 1940 2148 2350 434 Size: 1506 208 202 4692 Fragments arranged by size: 4692 1506 208 202 BsiEI CG_ry'CG Cuts at: 104 4150 4196 5404 104 Size: 4046 46 1208 1308 Fragments arranged by size: 4046 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 3622 4400 4590 5382 3622 Size: 778 190 792 4848 Fragments arranged by size: 4848 792 778 190 BsmI GAATG_Cn' Cuts at: 1363 1603 1363 Size: 240 6368 Bsp1286I G_dGCh'C Cuts at: 1562 2760 3415 3622 4233 4326 4400 4590 4652 Size: 1198 655 207 611 93 74 190 62 Cuts at: 4652 5382 1562 Size: 730 2788 Fragments arranged by size: 2788 1198 730 655 611 207 190 93 74 62 BspEI T'CCGG_A Cuts at: 5828 5828 Size: 6608 BspHI T'CATG_A Cuts at: 678 5784 678 Size: 5106 1502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2617 3658 3872 4174 4555 2617 Size: 1041 214 302 381 4670 Fragments arranged by size: 4670 1041 381 302 214 BsrBI CCG'CTC Cuts at: 50 4900 4954 6438 6603 50 Size: 4850 54 1484 165 55 Fragments arranged by size: 4850 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1353 2175 4520 1353 Size: 822 2345 3441 Fragments arranged by size: 3441 2345 822 BsrFI r'CCGG_y Cuts at: 4606 4787 4996 4606 Size: 181 209 6218 Fragments arranged by size: 6218 209 181 BssSI C'ACGA_G Cuts at: 1082 4879 5237 1082 Size: 3797 358 2453 Fragments arranged by size: 3797 2453 358 BstAPI GCAn_nnn'nTGC Cuts at: 1927 3985 1927 Size: 2058 4550 BstBI TT'CG_AA Cuts at: 286 4969 286 Size: 4683 1925 BstEII G'GTnAC_C Cuts at: 3021 3351 3021 Size: 330 6278 BstXI CCAn_nnnn'nTGG Cuts at: 807 1061 807 Size: 254 6354 BstYI r'GATC_y Cuts at: 1 2946 3633 4100 4123 4458 4704 5716 5802 Size: 2945 687 467 23 335 246 1012 86 Cuts at: 5802 5814 6160 1 Size: 12 346 449 Fragments arranged by size: 2945 1012 687 467 449 346 335 246 86 23 12 BstZ17I GTA'TAC Cuts at: 1000 1000 Size: 6608 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1351 4558 4739 4902 1351 Size: 3207 181 163 3057 Fragments arranged by size: 3207 3057 181 163 BtsI GCAGTG_nn' Cuts at: 660 678 1158 1340 1717 2136 3851 660 Size: 18 480 182 377 419 1715 3417 Fragments arranged by size: 3417 1715 480 419 377 182 18 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 2903 2938 2903 Size: 35 6573 DraI TTT'AAA Cuts at: 866 2354 3607 866 Size: 1488 1253 3867 Fragments arranged by size: 3867 1488 1253 DraIII CAC_nnn'GTG Cuts at: 1640 2341 2395 3140 1640 Size: 701 54 745 5108 Fragments arranged by size: 5108 745 701 54 DrdI GACnn_nn'nnGTC Cuts at: 967 3396 4314 5172 967 Size: 2429 918 858 2403 Fragments arranged by size: 2429 2403 918 858 EaeI y'GGCC_r Cuts at: 2252 4193 4367 4758 4785 6130 2252 Size: 1941 174 391 27 1345 2730 Fragments arranged by size: 2730 1941 1345 391 174 27 EagI C'GGCC_G Cuts at: 4193 4193 Size: 6608 EarI CTCTTCn'nnn_ Cuts at: 2478 2667 2843 4631 4841 2478 Size: 189 176 1788 210 4245 Fragments arranged by size: 4245 1788 210 189 176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4816 5126 5272 6418 4816 Size: 310 146 1146 5006 Fragments arranged by size: 5006 1146 310 146 EcoICRI GAG'CTC Cuts at: 3620 3620 Size: 6608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 626 1083 1092 3332 3439 4433 4865 5612 5880 Size: 457 9 2240 107 994 432 747 268 Cuts at: 5880 626 Size: 1354 Fragments arranged by size: 2240 1354 994 747 457 432 268 107 9 EcoNI CCTnn'n_nnAGG Cuts at: 2027 2027 Size: 6608 EcoO109I rG'GnC_Cy Cuts at: 2032 3747 2032 Size: 1715 4893 EcoRI G'AATT_C Cuts at: 933 3612 933 Size: 2679 3929 EcoRV GAT'ATC Cuts at: 2871 2871 Size: 6608 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1809 4627 1809 Size: 2818 3790 FspI TGC'GCA Cuts at: 4389 4389 Size: 6608 HaeII r_GCGC'y Cuts at: 4042 4290 5312 6291 4042 Size: 248 1022 979 4359 Fragments arranged by size: 4359 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1522 1554 1867 1899 2139 2171 2817 2849 3163 Size: 32 313 32 240 32 646 32 314 Cuts at: 3163 3195 3316 3348 3451 3483 4450 4480 4482 Size: 32 121 32 103 32 967 30 2 Cuts at: 4482 4512 1522 Size: 30 3618 Fragments arranged by size: 3618 967 646 314 313 240 121 103 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1457 2994 3647 1457 Size: 1537 653 4418 Fragments arranged by size: 4418 1537 653 HpaI GTT'AAC Cuts at: 2994 2994 Size: 6608 KasI G'GCGC_C Cuts at: 4038 4286 4038 Size: 248 6360 KpnI G_GTAC'C Cuts at: 898 3628 898 Size: 2730 3878 MfeI C'AATT_G Cuts at: 666 1495 2264 666 Size: 829 769 5010 Fragments arranged by size: 5010 829 769 MluI A'CGCG_T Cuts at: 6041 6041 Size: 6608 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 5279 5463 5279 Size: 184 6424 MscI TGG'CCA Cuts at: 2254 4369 2254 Size: 2115 4493 MslI CAynn'nnrTG Cuts at: 805 1370 1686 2618 4597 4724 805 Size: 565 316 932 1979 127 2689 Fragments arranged by size: 2689 1979 932 565 316 127 MspA1I CmG'CkG Cuts at: 307 3719 4033 4393 5406 5651 307 Size: 3412 314 360 1013 245 1264 Fragments arranged by size: 3412 1264 1013 360 314 245 NaeI GCC'GGC Cuts at: 4789 4789 Size: 6608 NarI GG'CG_CC Cuts at: 4039 4287 4039 Size: 248 6360 NdeI CA'TA_TG Cuts at: 839 839 Size: 6608 NgoMIV G'CCGG_C Cuts at: 4787 4787 Size: 6608 NsiI A_TGCA'T Cuts at: 1603 1603 Size: 6608 NspI r_CATG'y Cuts at: 222 1691 2831 3534 3661 4161 5068 222 Size: 1469 1140 703 127 500 907 1762 Fragments arranged by size: 1762 1469 1140 907 703 500 127 PciI A'CATG_T Cuts at: 1687 2827 5064 1687 Size: 1140 2237 3231 Fragments arranged by size: 3231 2237 1140 PfoI T'CCnGG_A Cuts at: 2377 3455 2377 Size: 1078 5530 PmeI GTTT'AAAC Cuts at: 3607 3607 Size: 6608 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5747 5779 5747 Size: 32 6576 PpuMI rG'GwC_Cy Cuts at: 2032 2032 Size: 6608 PsiI TTA'TAA Cuts at: 572 3121 572 Size: 2549 4059 PspXI vC'TCGA_Gb Cuts at: 1310 1310 Size: 6608 PstI C_TGCA'G Cuts at: 3655 4340 3655 Size: 685 5923 PvuI CG_AT'CG Cuts at: 104 104 Size: 6608 PvuII CAG'CTG Cuts at: 307 4033 4393 307 Size: 3726 360 2522 Fragments arranged by size: 3726 2522 360 RsrII CG'GwC_CG Cuts at: 4803 4803 Size: 6608 SacI G_AGCT'C Cuts at: 3622 3622 Size: 6608 SalI G'TCGA_C Cuts at: 3645 3645 Size: 6608 SapI GCTCTTCn'nnn_ Cuts at: 4631 4841 4631 Size: 210 6398 SbfI CC_TGCA'GG Cuts at: 3655 3655 Size: 6608 ScaI AGT'ACT Cuts at: 1077 6125 1077 Size: 5048 1560 SfcI C'TryA_G Cuts at: 33 3651 4336 5329 5520 33 Size: 3618 685 993 191 1121 Fragments arranged by size: 3618 1121 993 685 191 SfoI GGC'GCC Cuts at: 4040 4288 4040 Size: 248 6360 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6608 SmaI CCC'GGG Cuts at: 3630 3630 Size: 6608 SmlI C'TyrA_G Cuts at: 1310 2594 3763 4678 5170 5432 1310 Size: 1284 1169 915 492 262 2486 Fragments arranged by size: 2486 1284 1169 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6608 SphI G_CATG'C Cuts at: 222 3661 4161 222 Size: 3439 500 2669 Fragments arranged by size: 3439 2669 500 SspI AAT'ATT Cuts at: 925 2549 925 Size: 1624 4984 StyI C'CwwG_G Cuts at: 196 3742 4096 196 Size: 3546 354 2708 Fragments arranged by size: 3546 2708 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2406 4191 4324 4783 4992 2406 Size: 1785 133 459 209 4022 Fragments arranged by size: 4022 1785 459 209 133 TatI w'GTAC_w Cuts at: 1075 1337 1462 1848 6123 1075 Size: 262 125 386 4275 1560 Fragments arranged by size: 4275 1560 386 262 125 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6125 6157 6125 Size: 32 6576 Tth111I GACn'n_nGTC Cuts at: 4405 4405 Size: 6608 XbaI T'CTAG_A Cuts at: 3639 3639 Size: 6608 XcmI CCAnnnn_n'nnnnTGG Cuts at: 194 888 1825 4103 194 Size: 694 937 2278 2699 Fragments arranged by size: 2699 2278 937 694 XhoI C'TCGA_G Cuts at: 1310 1310 Size: 6608 XmaI C'CCGG_G Cuts at: 3628 3628 Size: 6608 XmnI GAAnn'nnTTC Cuts at: 559 2472 2729 559 Size: 1913 257 4438 Fragments arranged by size: 4438 1913 257 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PciI PfoI PmeI PpiI PpuMI PsiI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AloI ApaI AscI AvrII BcgI BglI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI BssHII Bsu36I BtgI ClaI FseI FspAI HindIII NcoI NheI NotI NruI PacI PasI PflMI PmlI PshAI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI ZraI