Restriction map for
pF1KE1599
(Linear) (Six-base) MAPSORT of: KIEE1599.seq Check: 6553 from: 1 to: 3495
KIEE1599.orf
With 182 enzymes: SgfI *
August 26, 2010 17:36 ..
AccI GT'mk_AC
Cuts at: 0 884 891 3495
Size: 884 7 2604
Fragments arranged by size:
2604 884 7
Acc65I G'GTAC_C
Cuts at: 0 786 3495
Size: 786 2709
AclI AA'CG_TT
Cuts at: 0 2680 3495
Size: 2680 815
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 3224 3495
Size: 518 2706 271
Fragments arranged by size:
2706 518 271
AflIII A'CryG_T
Cuts at: 0 1579 2719 3495
Size: 1579 1140 776
Fragments arranged by size:
1579 1140 776
AhdI GACnn_n'nnGTC
Cuts at: 0 2723 3495
Size: 2723 772
AleI CACnn'nnGTG
Cuts at: 0 2510 3495
Size: 2510 985
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1248 3495
Size: 1248 2247
ApoI r'AATT_y
Cuts at: 0 13 40 120 174 345 825 1016 1110
Size: 13 27 80 54 171 480 191 94
Cuts at: 1110 1124 1930 2098 2175 3048 3195 3495
Size: 14 806 168 77 873 147 300
Fragments arranged by size:
873 806 480 300 191 171 168 147
94 80 77 54 27 14 13
AseI AT'TA_AT
Cuts at: 0 1946 2474 2581 3495
Size: 1946 528 107 914
Fragments arranged by size:
1946 914 528 107
AvaI C'yCGr_G
Cuts at: 0 1202 3495
Size: 1202 2293
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2897 2930 3495
Size: 2897 33 565
Fragments arranged by size:
2897 565 33
BanI G'GyrC_C
Cuts at: 0 786 3495
Size: 786 2709
BanII G_rGCy'C
Cuts at: 0 1454 3495
Size: 1454 2041
BbsI GAAGACnn'nnnn_
Cuts at: 0 896 3412 3495
Size: 896 2516 83
Fragments arranged by size:
2516 896 83
BbvCI CC'TCA_GC
Cuts at: 0 2900 3495
Size: 2900 595
BciVI GTATCCnnnnn_n'
Cuts at: 0 2267 3495
Size: 2267 1228
BclI T'GATC_A
Cuts at: 0 1155 2394 2966 3495
Size: 1155 1239 572 529
Fragments arranged by size:
1239 1155 572 529
BfrBI ATG'CAT
Cuts at: 0 1493 3495
Size: 1493 2002
BglII A'GATC_T
Cuts at: 0 2838 3495
Size: 2838 657
Bme1580I G_kGCm'C
Cuts at: 0 2652 3307 3495
Size: 2652 655 188
Fragments arranged by size:
2652 655 188
BmrI ACTGGGnnnn_n'
Cuts at: 0 1789 1864 2279 3495
Size: 1789 75 415 1216
Fragments arranged by size:
1789 1216 415 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 989 3495
Size: 989 2506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 975 984 3331 3495
Size: 975 9 2347 164
Fragments arranged by size:
2347 975 164 9
Bpu10I CC'TnA_GC
Cuts at: 0 2900 3495
Size: 2900 595
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2471 3495
Size: 2471 1024
BsaI GGTCTCn'nnnn_
Cuts at: 0 2823 3495
Size: 2823 672
BsaBI GATnn'nnATC
Cuts at: 0 1540 3125 3495
Size: 1540 1585 370
Fragments arranged by size:
1585 1540 370
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 962 3495
Size: 962 2533
BseYI C'CCAG_C
Cuts at: 0 3312 3495
Size: 3312 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 1832 2040 2242 3495
Size: 326 1506 208 202 1253
Fragments arranged by size:
1506 1253 326 208 202
BsmI GAATG_Cn'
Cuts at: 0 1255 1495 3495
Size: 1255 240 2000
Fragments arranged by size:
2000 1255 240
Bsp1286I G_dGCh'C
Cuts at: 0 1454 2652 3307 3495
Size: 1454 1198 655 188
Fragments arranged by size:
1454 1198 655 188
BspHI T'CATG_A
Cuts at: 0 570 3495
Size: 570 2925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2509 3495
Size: 2509 986
BsrDI GCAATG_nn'
Cuts at: 0 1245 2067 3495
Size: 1245 822 1428
Fragments arranged by size:
1428 1245 822
BssSI C'ACGA_G
Cuts at: 0 974 3495
Size: 974 2521
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1819 3495
Size: 1819 1676
BstBI TT'CG_AA
Cuts at: 0 178 3495
Size: 178 3317
BstEII G'GTnAC_C
Cuts at: 0 2913 3243 3495
Size: 2913 330 252
Fragments arranged by size:
2913 330 252
BstXI CCAn_nnnn'nTGG
Cuts at: 0 699 953 3495
Size: 699 254 2542
Fragments arranged by size:
2542 699 254
BstYI r'GATC_y
Cuts at: 0 2838 3495
Size: 2838 657
BstZ17I GTA'TAC
Cuts at: 0 892 3495
Size: 892 2603
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1243 3495
Size: 1243 2252
BtsI GCAGTG_nn'
Cuts at: 0 552 570 1050 1232 1609 2028 3495
Size: 552 18 480 182 377 419 1467
Fragments arranged by size:
1467 552 480 419 377 182 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2795 2830 3495
Size: 2795 35 665
Fragments arranged by size:
2795 665 35
DraI TTT'AAA
Cuts at: 0 758 2246 3495
Size: 758 1488 1249
Fragments arranged by size:
1488 1249 758
DraIII CAC_nnn'GTG
Cuts at: 0 1532 2233 2287 3032 3495
Size: 1532 701 54 745 463
Fragments arranged by size:
1532 745 701 463 54
DrdI GACnn_nn'nnGTC
Cuts at: 0 859 3288 3495
Size: 859 2429 207
Fragments arranged by size:
2429 859 207
EaeI y'GGCC_r
Cuts at: 0 2144 3495
Size: 2144 1351
EarI CTCTTCn'nnn_
Cuts at: 0 2370 2559 2735 3495
Size: 2370 189 176 760
Fragments arranged by size:
2370 760 189 176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 975 984 3224 3331 3495
Size: 518 457 9 2240 107 164
Fragments arranged by size:
2240 518 457 164 107 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1919 3495
Size: 1919 1576
EcoO109I rG'GnC_Cy
Cuts at: 0 1924 3495
Size: 1924 1571
EcoRI G'AATT_C
Cuts at: 0 825 3495
Size: 825 2670
EcoRV GAT'ATC
Cuts at: 0 2763 3495
Size: 2763 732
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1701 3495
Size: 1701 1794
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1414 1446 1759 1791 2031 2063 2709 2741
Size: 1414 32 313 32 240 32 646 32
Cuts at: 2741 3055 3087 3208 3240 3343 3375 3495
Size: 314 32 121 32 103 32 120
Fragments arranged by size:
1414 646 314 313 240 121 120 103
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1349 2886 3495
Size: 1349 1537 609
Fragments arranged by size:
1537 1349 609
HpaI GTT'AAC
Cuts at: 0 2886 3495
Size: 2886 609
KpnI G_GTAC'C
Cuts at: 0 790 3495
Size: 790 2705
MfeI C'AATT_G
Cuts at: 0 558 1387 2156 3495
Size: 558 829 769 1339
Fragments arranged by size:
1339 829 769 558
MscI TGG'CCA
Cuts at: 0 2146 3495
Size: 2146 1349
MslI CAynn'nnrTG
Cuts at: 0 697 1262 1578 2510 3495
Size: 697 565 316 932 985
Fragments arranged by size:
985 932 697 565 316
MspA1I CmG'CkG
Cuts at: 0 199 3495
Size: 199 3296
NdeI CA'TA_TG
Cuts at: 0 731 3495
Size: 731 2764
NsiI A_TGCA'T
Cuts at: 0 1495 3495
Size: 1495 2000
NspI r_CATG'y
Cuts at: 0 114 1583 2723 3426 3495
Size: 114 1469 1140 703 69
Fragments arranged by size:
1469 1140 703 114 69
PciI A'CATG_T
Cuts at: 0 1579 2719 3495
Size: 1579 1140 776
Fragments arranged by size:
1579 1140 776
PfoI T'CCnGG_A
Cuts at: 0 2269 3347 3495
Size: 2269 1078 148
Fragments arranged by size:
2269 1078 148
PpuMI rG'GwC_Cy
Cuts at: 0 1924 3495
Size: 1924 1571
PsiI TTA'TAA
Cuts at: 0 464 3013 3495
Size: 464 2549 482
Fragments arranged by size:
2549 482 464
PspXI vC'TCGA_Gb
Cuts at: 0 1202 3495
Size: 1202 2293
PvuII CAG'CTG
Cuts at: 0 199 3495
Size: 199 3296
ScaI AGT'ACT
Cuts at: 0 969 3495
Size: 969 2526
SmlI C'TyrA_G
Cuts at: 0 1202 2486 3495
Size: 1202 1284 1009
Fragments arranged by size:
1284 1202 1009
SphI G_CATG'C
Cuts at: 0 114 3495
Size: 114 3381
SspI AAT'ATT
Cuts at: 0 817 2441 3495
Size: 817 1624 1054
Fragments arranged by size:
1624 1054 817
StyI C'CwwG_G
Cuts at: 0 88 3495
Size: 88 3407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2298 3495
Size: 2298 1197
TatI w'GTAC_w
Cuts at: 0 967 1229 1354 1740 3495
Size: 967 262 125 386 1755
Fragments arranged by size:
1755 967 386 262 125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 86 780 1717 3495
Size: 86 694 937 1778
Fragments arranged by size:
1778 937 694 86
XhoI C'TCGA_G
Cuts at: 0 1202 3495
Size: 1202 2293
XmnI GAAnn'nnTTC
Cuts at: 0 451 2364 2621 3495
Size: 451 1913 257 874
Fragments arranged by size:
1913 874 451 257
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
ApoI AseI AvaI BaeI BanI BanII BbsI BbvCI
BciVI BclI BfrBI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BseRI BseYI BsgI BsmI
Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HincII HpaI KpnI MfeI MscI MslI
MspA1I NdeI NsiI NspI PciI PfoI PpuMI PsiI
PspXI PvuII ScaI SmlI SphI SspI StyI TaqII
TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AloI AlwNI ApaI
ApaLI AscI AsiSI AvrII BamHI BbeI BcgI BglI
BlpI BmgBI BmtI BsaAI BsaHI BsaWI BsaXI BsiEI
BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
Bsu36I BtgI ClaI EagI EciI EcoICRI FseI FspI
FspAI HaeII HindIII KasI MluI MmeI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PflMI
PmeI PmlI PpiI PshAI PspOMI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI TstI Tth111I XbaI XmaI ZraI