(Circular) (Six-base) MAPSORT of: pF1KE2429.seq Check: 6573 from: 1 to: 5087 pF1KE2429 5087 bp With 182 enzymes: SgfI * December 4, 2012 11:59 .. AccI GT'mk_AC Cuts at: 2125 2125 Size: 5087 Acc65I G'GTAC_C Cuts at: 2103 2103 Size: 5087 AclI AA'CG_TT Cuts at: 4927 4927 Size: 5087 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 616 946 1495 1675 2912 3344 4091 4359 616 Size: 330 549 180 1237 432 747 268 1344 Fragments arranged by size: 1344 1237 747 549 432 330 268 180 AflIII A'CryG_T Cuts at: 2045 2439 3543 4520 2045 Size: 394 1104 977 2612 Fragments arranged by size: 2612 1104 977 394 AgeI A'CCGG_T Cuts at: 3475 3475 Size: 5087 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 241 422 241 Size: 181 4906 AlwNI CAG_nnn'CTG Cuts at: 210 1023 2392 3959 210 Size: 813 1369 1567 1338 Fragments arranged by size: 1567 1369 1338 813 ApaI G_GGCC'C Cuts at: 148 200 148 Size: 52 5035 ApaLI G'TGCA_C Cuts at: 305 3857 305 Size: 3552 1535 ApoI r'AATT_y Cuts at: 855 1468 2091 4440 4696 855 Size: 613 623 2349 256 1246 Fragments arranged by size: 2349 1246 623 613 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5087 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5087 AvaI C'yCGr_G Cuts at: 140 476 557 958 2006 2107 140 Size: 336 81 401 1048 101 3120 Fragments arranged by size: 3120 1048 401 336 101 81 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 288 321 288 Size: 33 5054 BamHI G'GATC_C Cuts at: 2112 2112 Size: 5087 BanI G'GyrC_C Cuts at: 111 2103 2517 2765 2800 111 Size: 1992 414 248 35 2398 Fragments arranged by size: 2398 1992 414 248 35 BanII G_rGCy'C Cuts at: 148 168 200 904 1013 1495 1667 1826 2101 Size: 20 32 704 109 482 172 159 275 Cuts at: 2101 3131 148 Size: 1030 2104 Fragments arranged by size: 2104 1030 704 482 275 172 159 109 32 20 BbeI G_GCGC'C Cuts at: 2521 2769 2521 Size: 248 4839 BbsI GAAGACnn'nnnn_ Cuts at: 287 604 2048 287 Size: 317 1444 3326 Fragments arranged by size: 3326 1444 317 BbvCI CC'TCA_GC Cuts at: 181 181 Size: 5087 BciVI GTATCCnnnnn_n' Cuts at: 746 2985 3746 5087 746 Size: 2239 761 1341 746 Fragments arranged by size: 2239 1341 761 746 BclI T'GATC_A Cuts at: 2607 2607 Size: 5087 BglI GCCn_nnn'nGGC Cuts at: 1513 1671 1513 Size: 158 4929 BglII A'GATC_T Cuts at: 2602 4639 2602 Size: 2037 3050 BlpI GC'TnA_GC Cuts at: 1489 2199 1489 Size: 710 4377 Bme1580I G_kGCm'C Cuts at: 116 148 200 230 309 2712 2805 3861 116 Size: 32 52 30 79 2403 93 1056 1342 Fragments arranged by size: 2403 1342 1056 93 79 52 32 30 BmrI ACTGGGnnnn_n' Cuts at: 164 747 788 2299 2380 2715 4859 164 Size: 583 41 1511 81 335 2144 392 Fragments arranged by size: 2144 1511 583 392 335 81 41 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3454 3454 Size: 5087 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 640 1653 640 Size: 1013 4074 Bpu10I CC'TnA_GC Cuts at: 181 1012 1397 1820 181 Size: 831 385 423 3448 Fragments arranged by size: 3448 831 423 385 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2263 3142 3634 3932 2263 Size: 879 492 298 3418 Fragments arranged by size: 3418 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1600 1929 4791 1600 Size: 329 2862 1896 Fragments arranged by size: 2862 1896 329 BsaAI yAC'GTr Cuts at: 2440 2440 Size: 5087 BsaBI GATnn'nnATC Cuts at: 6 2601 4638 6 Size: 2595 2037 455 Fragments arranged by size: 2595 2037 455 BsaHI Gr'CG_yC Cuts at: 2518 2766 3468 4964 2518 Size: 248 702 1496 2641 Fragments arranged by size: 2641 1496 702 248 BsaWI w'CCGG_w Cuts at: 587 2276 2797 3475 3749 3896 4307 587 Size: 1689 521 678 274 147 411 1367 Fragments arranged by size: 1689 1367 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1057 1472 1057 Size: 415 4672 BseYI C'CCAG_C Cuts at: 1508 1890 2512 3270 3847 4343 1508 Size: 382 622 758 577 496 2252 Fragments arranged by size: 2252 758 622 577 496 382 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1363 1363 Size: 5087 BsiEI CG_ry'CG Cuts at: 104 1599 2629 2675 3883 104 Size: 1495 1030 46 1208 1308 Fragments arranged by size: 1495 1308 1208 1030 46 BsiHKAI G_wGCw'C Cuts at: 309 1013 1223 1250 1645 1714 1826 1879 2101 Size: 704 210 27 395 69 112 53 222 Cuts at: 2101 2879 3069 3861 309 Size: 778 190 792 1535 Fragments arranged by size: 1535 792 778 704 395 222 210 190 112 69 53 27 BsmI GAATG_Cn' Cuts at: 1516 1585 1516 Size: 69 5018 Bsp1286I G_dGCh'C Cuts at: 116 148 168 200 230 309 904 1013 1223 Size: 32 20 32 30 79 595 109 210 Cuts at: 1223 1250 1495 1645 1667 1714 1826 1879 2101 Size: 27 245 150 22 47 112 53 222 Cuts at: 2101 2712 2805 2879 3069 3131 3861 116 Size: 611 93 74 190 62 730 1342 Fragments arranged by size: 1342 730 611 595 245 222 210 190 150 112 109 93 79 74 62 53 47 32 32 30 27 22 20 BspEI T'CCGG_A Cuts at: 4307 4307 Size: 5087 BspHI T'CATG_A Cuts at: 1956 4263 1956 Size: 2307 2780 BspMI ACCTGCnnnn'nnnn_ Cuts at: 218 2137 2351 2653 3034 218 Size: 1919 214 302 381 2271 Fragments arranged by size: 2271 1919 381 302 214 BsrBI CCG'CTC Cuts at: 50 3379 3433 4917 5082 50 Size: 3329 54 1484 165 55 Fragments arranged by size: 3329 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1044 1754 1983 2007 2999 1044 Size: 710 229 24 992 3132 Fragments arranged by size: 3132 992 710 229 24 BsrFI r'CCGG_y Cuts at: 187 3085 3266 3475 187 Size: 2898 181 209 1799 Fragments arranged by size: 2898 1799 209 181 BssHII G'CGCG_C Cuts at: 129 129 Size: 5087 BssSI C'ACGA_G Cuts at: 1872 3358 3716 1872 Size: 1486 358 3243 Fragments arranged by size: 3243 1486 358 BstAPI GCAn_nnn'nTGC Cuts at: 2464 2464 Size: 5087 BstBI TT'CG_AA Cuts at: 3448 3448 Size: 5087 BstYI r'GATC_y Cuts at: 1 2112 2579 2602 2937 3183 4195 4281 4293 Size: 2111 467 23 335 246 1012 86 12 Cuts at: 4293 4639 1 Size: 346 449 Fragments arranged by size: 2111 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 844 1051 844 Size: 207 4880 BtgI C'CryG_G Cuts at: 107 149 1722 107 Size: 42 1573 3472 Fragments arranged by size: 3472 1573 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3037 3218 3381 3037 Size: 181 163 4743 Fragments arranged by size: 4743 181 163 BtsI GCAGTG_nn' Cuts at: 1076 1250 1577 1901 2330 1076 Size: 174 327 324 429 3833 Fragments arranged by size: 3833 429 327 324 174 DraI TTT'AAA Cuts at: 1272 2086 1272 Size: 814 4273 DrdI GACnn_nn'nnGTC Cuts at: 1333 2793 3651 1333 Size: 1460 858 2769 Fragments arranged by size: 2769 1460 858 EaeI y'GGCC_r Cuts at: 201 330 1556 1596 1725 2672 2846 3237 3264 Size: 129 1226 40 129 947 174 391 27 Cuts at: 3264 4609 201 Size: 1345 679 Fragments arranged by size: 1345 1226 947 679 391 174 129 129 40 27 EagI C'GGCC_G Cuts at: 1596 2672 1596 Size: 1076 4011 EarI CTCTTCn'nnn_ Cuts at: 814 1989 3110 3320 814 Size: 1175 1121 210 2581 Fragments arranged by size: 2581 1175 1121 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1603 3295 3605 3751 4897 1603 Size: 1692 310 146 1146 1793 Fragments arranged by size: 1793 1692 1146 310 146 EcoICRI GAG'CTC Cuts at: 1011 1824 2099 1011 Size: 813 275 3999 Fragments arranged by size: 3999 813 275 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 616 640 946 1495 1653 1675 2912 3344 4091 Size: 24 306 549 158 22 1237 432 747 Cuts at: 4091 4359 616 Size: 268 1344 Fragments arranged by size: 1344 1237 747 549 432 306 268 158 24 22 EcoNI CCTnn'n_nnAGG Cuts at: 1656 1656 Size: 5087 EcoO109I rG'GnC_Cy Cuts at: 144 145 448 716 1107 2226 144 Size: 1 303 268 391 1119 3005 Fragments arranged by size: 3005 1119 391 303 268 1 EcoRI G'AATT_C Cuts at: 1468 2091 1468 Size: 623 4464 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 336 3106 336 Size: 2770 2317 FspI TGC'GCA Cuts at: 2868 2868 Size: 5087 HaeII r_GCGC'y Cuts at: 188 2521 2769 3791 4770 188 Size: 2333 248 1022 979 505 Fragments arranged by size: 2333 1022 979 505 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 517 549 2929 2959 2961 2991 517 Size: 32 2380 30 2 30 2613 Fragments arranged by size: 2613 2380 32 30 30 2 HincII GTy'rAC Cuts at: 2126 2126 Size: 5087 KasI G'GCGC_C Cuts at: 2517 2765 2517 Size: 248 4839 KpnI G_GTAC'C Cuts at: 2107 2107 Size: 5087 MluI A'CGCG_T Cuts at: 4520 4520 Size: 5087 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3758 3942 3758 Size: 184 4903 MscI TGG'CCA Cuts at: 332 1558 1727 2848 332 Size: 1226 169 1121 2571 Fragments arranged by size: 2571 1226 1121 169 MslI CAynn'nnrTG Cuts at: 1142 3076 3203 1142 Size: 1934 127 3026 Fragments arranged by size: 3026 1934 127 MspA1I CmG'CkG Cuts at: 151 402 1157 1393 1512 1731 2198 2512 2872 Size: 251 755 236 119 219 467 314 360 Cuts at: 2872 3885 4130 151 Size: 1013 245 1108 Fragments arranged by size: 1108 1013 755 467 360 314 251 245 236 219 119 NaeI GCC'GGC Cuts at: 189 3268 189 Size: 3079 2008 NarI GG'CG_CC Cuts at: 2518 2766 2518 Size: 248 4839 NcoI C'CATG_G Cuts at: 107 1722 107 Size: 1615 3472 NgoMIV G'CCGG_C Cuts at: 187 3266 187 Size: 3079 2008 NspI r_CATG'y Cuts at: 2049 2140 2640 3547 2049 Size: 91 500 907 3589 Fragments arranged by size: 3589 907 500 91 PciI A'CATG_T Cuts at: 2045 3543 2045 Size: 1498 3589 PflMI CCAn_nnn'nTGG Cuts at: 2025 2025 Size: 5087 PfoI T'CCnGG_A Cuts at: 159 875 159 Size: 716 4371 PmeI GTTT'AAAC Cuts at: 2086 2086 Size: 5087 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4226 4258 4226 Size: 32 5055 PpuMI rG'GwC_Cy Cuts at: 448 1107 448 Size: 659 4428 PspOMI G'GGCC_C Cuts at: 144 196 144 Size: 52 5035 PstI C_TGCA'G Cuts at: 215 2134 2819 215 Size: 1919 685 2483 Fragments arranged by size: 2483 1919 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 5087 PvuII CAG'CTG Cuts at: 402 1393 1512 1731 2512 2872 402 Size: 991 119 219 781 360 2617 Fragments arranged by size: 2617 991 781 360 219 119 RsrII CG'GwC_CG Cuts at: 3282 3282 Size: 5087 SacI G_AGCT'C Cuts at: 1013 1826 2101 1013 Size: 813 275 3999 Fragments arranged by size: 3999 813 275 SacII CC_GC'GG Cuts at: 152 152 Size: 5087 SalI G'TCGA_C Cuts at: 2124 2124 Size: 5087 SanDI GG'GwC_CC Cuts at: 448 448 Size: 5087 SapI GCTCTTCn'nnn_ Cuts at: 3110 3320 3110 Size: 210 4877 SbfI CC_TGCA'GG Cuts at: 2134 2134 Size: 5087 ScaI AGT'ACT Cuts at: 4604 4604 Size: 5087 SexAI A'CCwGG_T Cuts at: 722 722 Size: 5087 SfcI C'TryA_G Cuts at: 33 211 1572 2130 2815 3808 3999 33 Size: 178 1361 558 685 993 191 1121 Fragments arranged by size: 1361 1121 993 685 558 191 178 SfoI GGC'GCC Cuts at: 2519 2767 2519 Size: 248 4839 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5087 SmaI CCC'GGG Cuts at: 142 559 2008 2109 142 Size: 417 1449 101 3120 Fragments arranged by size: 3120 1449 417 101 SmlI C'TyrA_G Cuts at: 476 2242 3157 3649 3911 476 Size: 1766 915 492 262 1652 Fragments arranged by size: 1766 1652 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5087 SphI G_CATG'C Cuts at: 2140 2640 2140 Size: 500 4587 StuI AGG'CCT Cuts at: 1651 2035 1651 Size: 384 4703 StyI C'CwwG_G Cuts at: 107 1190 1722 1902 2221 2575 107 Size: 1083 532 180 319 354 2619 Fragments arranged by size: 2619 1083 532 354 319 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1781 2670 2803 3262 3471 1781 Size: 889 133 459 209 3397 Fragments arranged by size: 3397 889 459 209 133 TatI w'GTAC_w Cuts at: 929 4602 929 Size: 3673 1414 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4604 4636 4604 Size: 32 5055 Tth111I GACn'n_nGTC Cuts at: 1111 1441 2884 1111 Size: 330 1443 3314 Fragments arranged by size: 3314 1443 330 XbaI T'CTAG_A Cuts at: 2118 2118 Size: 5087 XcmI CCAnnnn_n'nnnnTGG Cuts at: 834 2582 834 Size: 1748 3339 XhoI C'TCGA_G Cuts at: 476 476 Size: 5087 XmaI C'CCGG_G Cuts at: 140 557 2006 2107 140 Size: 417 1449 101 3120 Fragments arranged by size: 3120 1449 417 101 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI AscI AvrII BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI