Restriction map for pF1KB7666
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# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AcyI        AflII       AgeI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BamHI       BanI        BbeI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MunI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SwaI        Tth111I     XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccIII         13  TCCGGA               T^CCGGA
AflIII        374  ACATGT               A^C[GA][CT]GT
AhdI          154  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         566  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          712  CCTCAGG              CC^T[ACGT]AGG
AspEI         154  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         487  GAGCAC               G[AT]GC[AT]^C
BalI          215  TGGCCA               TGG^CCA
BanII         241  GAGCCC               G[GA]GC[CT]^C
BbrPI         757  CACGTG               CAC^GTG
BbsI          162  GTCTTC               GAAGAC(2/6)
BbvCI         766  CCTCAGC              CCTCAGC(-5/-2)
BglII         107  AGATCT               A^GATCT
BpuAI         162  GTCTTC               GAAGAC(2/6)
BsaAI         757  CACGTG               [CT]AC^GT[GA]
BsaWI          13  TCCGGA               [AT]^CCGG[AT]
BseAI          13  TCCGGA               T^CCGGA
BseRI          17  GAGGAG               GAGGAG(10/8)
BsiHKAI       487  GAGCAC               G[AT]GC[AT]^C
BsmI          825  GAATGC               GAATGC(1/-1)
BspEI          13  TCCGGA               T^CCGGA
BspLU11I      374  ACATGT               A^CATGT
BsrI          682  ACTGG                ACTGG(1/-1)
BssSI         208  CACGAG               CACGAG(-5/-1)
Bsu36I        712  CCTCAGG              CC^T[ACGT]AGG
CfoI           53  GCGC                 GCG^C
DraIII        208  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      154  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        712  CCTCAGG              CC^T[ACGT]AGG
EcoRV         574  GATATC               GAT^ATC
EcoT14I       167  CCTTGG               C^C[AT][AT]GG
FauI           32  GCGGG                CCCGC(4/6)
HhaI           53  GCGC                 GCG^C
HinP1I         53  GCGC                 G^CGC
HincII        532  GTCAAC               GT[CT]^[GA]AC
HindII        532  GTCAAC               GT[CT]^[GA]AC
MaeII         758  ACGT                 A^CGT
MluNI         215  TGGCCA               TGG^CCA
MroI           13  TCCGGA               T^CCGGA
MscI          215  TGGCCA               TGG^CCA
MseI          812  TTAA                 T^TAA
MspA1I       1100  CAGCTG               C[AC]G^C[GT]G
PaeR7I        324  CTCGAG               C^TCGAG
PflMI         625  CCACCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          178  GAGTC                GAGTC(4/5)
PmaCI         757  CACGTG               CAC^GTG
PmlI          757  CACGTG               CAC^GTG
PvuII        1100  CAGCTG               CAG^CTG
SapI          943  GAAGAGC              GCTCTTC(1/4)
SfcI         1063  CTACAG               C^T[GA][CT]AG
SmlI          324  CTCGAG               C^T[CT][GA]AG
SphI          927  GCATGC               GCATG^C
SspI          956  AATATT               AAT^ATT
StuI          297  AGGCCT               AGG^CCT
StyI          167  CCTTGG               C^C[AT][AT]GG
TaqI          325  TCGA                 T^CGA
Tru9I         812  TTAA                 T^TAA
TthHB8I       325  TCGA                 T^CGA
Van91I        625  CCACCTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          625  CCACCTCTTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          324  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AvaI          324  CTCGAG               C^[CT]CG[GA]G
              405  CCCGAG              
BpmI          497  CTCCAG               CTGGAG(16/14)
              563  CTCCAG              
BsgI          433  GTGCAG               GTGCAG(16/14)
              893  GTGCAG              
BsmAI        1022  GAGAC                GTCTC(1/5)
              597  GTCTC               
BsoBI         324  CTCGAG               C^[CT]CG[GA]G
              405  CCCGAG              
BstXI         675  CCACACAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              906  CCAGATTCTTGG        
BstYI         107  AGATCT               [GA]^GATC[CT]
              385  GGATCT              
DraII          38  GGGACCC              [GA]G^G[ACGT]CC[CT]
              312  GGGACCC             
DsaI           56  CCACGG               C^C[GA][CT]GG
              428  CCGTGG              
EaeI          194  CGGCCA               [CT]^GGCC[GA]
              215  TGGCCA              
EcoO109I       38  GGGACCC              [GA]G^G[ACGT]CC[CT]
              312  GGGACCC             
MflI          107  AGATCT               [GA]^GATC[CT]
              385  GGATCT              
NdeI          649  CATATG               CA^TATG
              991  CATATG              
PpuMI          38  GGGACCC              [GA]G^G[AT]CC[CT]
              312  GGGACCC             
Tsp509I       965  AATT                 ^AATT
             1027  AATT                
XhoII         107  AGATCT               [GA]^GATC[CT]
              385  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BbvI        BcnI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TfiI        
TseI        Tsp45I      TspRI       
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