Restriction map for pF1KE3815
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        AhdI        AlwNI       Aor51HI     
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BbsI        
BcgI        BciVI       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaI        BsiWI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrDI       
BsrGI       BsrI        Bst1107I    BstAPI      BstBI       
BstXI       BstZ17I     CelII       ClaI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
MfeI        MluI        MseI        MunI        NcoI        
NdeI        NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        SalI        SapI        
SbfI        ScaI        SexAI       SfuI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcsI          160  AAATTC               [GA]^AATT[CT]
AfaI          309  GTAC                 GT^AC
AocI          943  CCTCAGG              CC^T[ACGT]AGG
ApoI          160  AAATTC               [GA]^AATT[CT]
BalI          563  TGGCCA               TGG^CCA
BamHI         490  GGATCC               G^GATCC
BbrPI         763  CACGTG               CAC^GTG
BfaI         1354  CTAG                 C^TAG
BglII         599  AGATCT               A^GATCT
BsaAI         763  CACGTG               [CT]AC^GT[GA]
BsaBI         594  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         166  CGGCCG               CG[GA][CT]^CG
BsmI          353  GCATTC               GAATGC(1/-1)
BssSI         708  CTCGTG               CACGAG(-5/-1)
BstEII        694  GGTCACC              G^GT[ACGT]ACC
BstPI         694  GGTCACC              G^GT[ACGT]ACC
Bsu36I        943  CCTCAGG              CC^T[ACGT]AGG
CpoI          475  CGGACCG              CG^G[AT]CCG
EagI          166  CGGCCG               C^GGCCG
EarI         1390  GAAGAG               CTCTTC(1/4)
EclXI         166  CGGCCG               C^GGCCG
Eco52I        166  CGGCCG               C^GGCCG
Eco81I        943  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       694  GGTCACC              G^GT[ACGT]ACC
EcoT14I      1068  CCAAGG               C^C[AT][AT]GG
Ksp632I      1390  GAAGAG               CTCTTC(1/4)
KspI          218  CCGCGG               CCGC^GG
MaeI         1354  CTAG                 C^TAG
MamI          594  GATTCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         563  TGGCCA               TGG^CCA
MscI          563  TGGCCA               TGG^CCA
MslI          177  CACCGCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI         763  CACGTG               CAC^GTG
PmlI          763  CACGTG               CAC^GTG
PshAI         477  GACCGCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         403  CAGCTG               CAG^CTG
RsaI          309  GTAC                 GT^AC
RsrII         475  CGGACCG              CG^G[AT]CCG
SacI         1309  GAGCTC               GAGCT^C
SacII         218  CCGCGG               CCGC^GG
SfiI         1009  GGCCTGCCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI         1049  CTCAAG               C^T[CT][GA]AG
StyI         1068  CCAAGG               C^C[AT][AT]GG
TaqI          615  TCGA                 T^CGA
Tsp509I       161  AATT                 ^AATT
TspRI          95  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       615  TCGA                 T^CGA
XcmI          326  CCAGAGAACGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccIII        357  TCCGGA               T^CCGGA
              921  TCCGGA              
Alw44I         97  GTGCAC               G^TGCAC
              115  GTGCAC              
ApaI         1017  GGGCCC               GGGCC^C
             1042  GGGCCC              
ApaLI          97  GTGCAC               G^TGCAC
              115  GTGCAC              
BseAI         357  TCCGGA               T^CCGGA
              921  TCCGGA              
BsmBI         444  GAGACG               CGTCTC(1/5)
              571  GAGACG              
BspEI         357  TCCGGA               T^CCGGA
              921  TCCGGA              
BspMI          87  ACCTGC               ACCTGC(4/8)
              876  ACCTGC              
BsrBI        1046  CCGCTC               CCGCTC(-3/-3)
              298  GAGCGG              
BssHII        773  GCGCGC               G^CGCGC
              886  GCGCGC              
BstF5I       1236  CATCC                GGATG(2/0)
             1305  GGATG               
DsaI          218  CCGCGG               C^C[GA][CT]GG
             1105  CCGTGG              
EaeI          166  CGGCCG               [CT]^GGCC[GA]
              563  TGGCCA              
EcoNI          31  CCTTTACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1416  CCTCAGCGAGG         
FokI         1236  CATCC                GGATG(9/13)
             1305  GGATG               
MaeII         764  ACGT                 A^CGT
             1090  ACGT                
MboII        1390  GAAGA                GAAGA(8/7)
             1426  GAAGA               
MroI          357  TCCGGA               T^CCGGA
              921  TCCGGA              
MspA1I        218  CCGCGG               C[AC]G^C[GT]G
              403  CAGCTG              
PpuMI         992  GGGACCC              [GA]G^G[AT]CC[CT]
             1125  GGGTCCC             
PstI          835  CTGCAG               CTGCA^G
             1333  CTGCAG              
SfcI          835  CTGCAG               C^T[GA][CT]AG
             1333  CTGCAG              
SgrAI         415  CACCGGCG             C[GA]^CCGG[CT]G
              729  CACCGGCG            
StuI           72  AGGCCT               AGG^CCT
             1382  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AspHI       AvaI        AvaII       BanI        BanII       
BbeI        BbvCI       BbvI        BcnI        BglI        
BmyI        BpmI        BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrFI       
BssKI       BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      FauI        Fnu4HI      
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        MaeIII      MboI        MflI        
MnlI        MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIV       PleI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfoI        SmaI        TfiI        TseI        
Tsp45I      XhoII       XmaCI       XmaI        
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