Restriction map for pF1KE3815
 (Linear) (Six-base) MAPSORT of: KIEE3815.seq  Check: 7558  from: 1  to: 1455



KIEE3815.orf

 With 182 enzymes: SgfI * 

                        July 24, 2018 16:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      96    1455 
   Size:         96    1359
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1305    1455 
   Size:       1305     150
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     735     767    1455 
   Size:        735      32     688

  Fragments arranged by size: 

                 735     688      32
 
ApaI G_GGCC'C

Cuts at:      0    1021    1046    1455 
   Size:       1021      25     409

  Fragments arranged by size: 

                1021     409      25
 
ApaLI G'TGCA_C

Cuts at:      0      97     115    1455 
   Size:         97      18    1340

  Fragments arranged by size: 

                1340      97      18
 
ApoI r'AATT_y

Cuts at:      0     160    1455 
   Size:        160    1295
 
AvaI C'yCGr_G

Cuts at:      0     227     264     312     439     551     630     969    1368 
   Size:        227      37      48     127     112      79     339     399

Cuts at:   1368    1455 
   Size:         87

  Fragments arranged by size: 

                 399     339     227     127     112      87      79      48
                  37
 
BamHI G'GATC_C

Cuts at:      0     490    1455 
   Size:        490     965
 
BanI G'GyrC_C

Cuts at:      0     626     648    1242    1269    1455 
   Size:        626      22     594      27     186

  Fragments arranged by size: 

                 626     594     186      27      22
 
BanII G_rGCy'C

Cuts at:      0     127     176     365     786    1021    1046    1313    1324 
   Size:        127      49     189     421     235      25     267      11

Cuts at:   1324    1440    1455 
   Size:        116      15

  Fragments arranged by size: 

                 421     267     235     189     127     116      49      25
                  15      11
 
BbeI G_GCGC'C

Cuts at:      0     652    1246    1273    1455 
   Size:        652     594      27     182

  Fragments arranged by size: 

                 652     594     182      27
 
BbvCI CC'TCA_GC

Cuts at:      0     110     406    1417    1455 
   Size:        110     296    1011      38

  Fragments arranged by size: 

                1011     296     110      38
 
BglI GCCn_nnn'nGGC

Cuts at:      0     856     913    1016    1144    1455 
   Size:        856      57     103     128     311

  Fragments arranged by size: 

                 856     311     128     103      57
 
BglII A'GATC_T

Cuts at:      0     599    1455 
   Size:        599     856
 
Bme1580I G_kGCm'C

Cuts at:      0     101     119     770    1021    1046    1455 
   Size:        101      18     651     251      25     409

  Fragments arranged by size: 

                 651     409     251     101      25      18
 
BmgBI CAC'GTC

Cuts at:      0    1091    1455 
   Size:       1091     364
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     885    1416    1455 
   Size:        885     531      39

  Fragments arranged by size: 

                 885     531      39
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1243    1399    1455 
   Size:       1243     156      56

  Fragments arranged by size: 

                1243     156      56
 
Bpu10I CC'TnA_GC

Cuts at:      0     110     406    1417    1455 
   Size:        110     296    1011      38

  Fragments arranged by size: 

                1011     296     110      38
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1034    1455 
   Size:       1034     421
 
BsaAI yAC'GTr

Cuts at:      0     765    1455 
   Size:        765     690
 
BsaBI GATnn'nnATC

Cuts at:      0     598    1455 
   Size:        598     857
 
BsaHI Gr'CG_yC

Cuts at:      0     649    1199    1243    1270    1455 
   Size:        649     550      44      27     185

  Fragments arranged by size: 

                 649     550     185      44      27
 
BsaWI w'CCGG_w

Cuts at:      0     357     487     921    1455 
   Size:        357     130     434     534

  Fragments arranged by size: 

                 534     434     357     130
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     499     571     908     941    1455 
   Size:        499      72     337      33     514

  Fragments arranged by size: 

                 514     499     337      72      33
 
BseYI C'CCAG_C

Cuts at:      0     841    1115    1181    1455 
   Size:        841     274      66     274

  Fragments arranged by size: 

                 841     274     274      66
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     693    1265    1455 
   Size:         68     625     572     190

  Fragments arranged by size: 

                 625     572     190      68
 
BsiEI CG_ry'CG

Cuts at:      0     169    1455 
   Size:        169    1286
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     119    1313    1455 
   Size:        101      18    1194     142

  Fragments arranged by size: 

                1194     142     101      18
 
BsmI GAATG_Cn'

Cuts at:      0     353    1455 
   Size:        353    1102
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     438     565    1455 
   Size:        438     127     890

  Fragments arranged by size: 

                 890     438     127
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     119     127     176     365     770     786    1021 
   Size:        101      18       8      49     189     405      16     235

Cuts at:   1021    1046    1313    1324    1440    1455 
   Size:         25     267      11     116      15

  Fragments arranged by size: 

                 405     267     235     189     116     101      49      25
                  18      16      15      11       8
 
BspEI T'CCGG_A

Cuts at:      0     357     921    1455 
   Size:        357     564     534

  Fragments arranged by size: 

                 564     534     357
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      96     885    1455 
   Size:         96     789     570

  Fragments arranged by size: 

                 789     570      96
 
BsrBI CCG'CTC

Cuts at:      0     300    1048    1455 
   Size:        300     748     407

  Fragments arranged by size: 

                 748     407     300
 
BsrFI r'CCGG_y

Cuts at:      0     129     416     464     730     855    1266    1455 
   Size:        129     287      48     266     125     411     189

  Fragments arranged by size: 

                 411     287     266     189     129     125      48
 
BssHII G'CGCG_C

Cuts at:      0     773     886    1455 
   Size:        773     113     569

  Fragments arranged by size: 

                 773     569     113
 
BssSI C'ACGA_G

Cuts at:      0     708    1455 
   Size:        708     747
 
BstEII G'GTnAC_C

Cuts at:      0     694    1455 
   Size:        694     761
 
BstYI r'GATC_y

Cuts at:      0     490     599     866    1455 
   Size:        490     109     267     589

  Fragments arranged by size: 

                 589     490     267     109
 
Bsu36I CC'TnA_GG

Cuts at:      0     944    1455 
   Size:        944     511
 
BtgI C'CryG_G

Cuts at:      0     218    1105    1455 
   Size:        218     887     350

  Fragments arranged by size: 

                 887     350     218
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     196     303     528    1455 
   Size:        196     107     225     927

  Fragments arranged by size: 

                 927     225     196     107
 
BtsI GCAGTG_nn'

Cuts at:      0     101    1455 
   Size:        101    1354
 
EaeI y'GGCC_r

Cuts at:      0     166     563    1455 
   Size:        166     397     892

  Fragments arranged by size: 

                 892     397     166
 
EagI C'GGCC_G

Cuts at:      0     166    1455 
   Size:        166    1289
 
EarI CTCTTCn'nnn_

Cuts at:      0    1385    1455 
   Size:       1385      70
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1194    1455 
   Size:       1194     261
 
EcoICRI GAG'CTC

Cuts at:      0    1311    1455 
   Size:       1311     144
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1243    1399    1455 
   Size:       1243     156      56

  Fragments arranged by size: 

                1243     156      56
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      35    1420    1455 
   Size:         35    1385      35

  Fragments arranged by size: 

                1385      35      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0     279     993    1018    1126    1365    1455 
   Size:        279     714      25     108     239      90

  Fragments arranged by size: 

                 714     279     239     108      90      25
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     549    1093    1455 
   Size:        549     544     362

  Fragments arranged by size: 

                 549     544     362
 
HaeII r_GCGC'y

Cuts at:      0     652     934    1246    1258    1273    1455 
   Size:        652     282     312      12      15     182

  Fragments arranged by size: 

                 652     312     282     182      15      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     236     268    1455 
   Size:        236      32    1187

  Fragments arranged by size: 

                1187     236      32
 
KasI G'GCGC_C

Cuts at:      0     648    1242    1269    1455 
   Size:        648     594      27     186

  Fragments arranged by size: 

                 648     594     186      27
 
MscI TGG'CCA

Cuts at:      0     565    1455 
   Size:        565     890
 
MslI CAynn'nnrTG

Cuts at:      0     181    1455 
   Size:        181    1274
 
MspA1I CmG'CkG

Cuts at:      0     220     405    1455 
   Size:        220     185    1050

  Fragments arranged by size: 

                1050     220     185
 
NaeI GCC'GGC

Cuts at:      0     131     466     857    1268    1455 
   Size:        131     335     391     411     187

  Fragments arranged by size: 

                 411     391     335     187     131
 
NarI GG'CG_CC

Cuts at:      0     649    1243    1270    1455 
   Size:        649     594      27     185

  Fragments arranged by size: 

                 649     594     185      27
 
NgoMIV G'CCGG_C

Cuts at:      0     129     464     855    1266    1455 
   Size:        129     335     391     411     189

  Fragments arranged by size: 

                 411     391     335     189     129
 
PasI CC'CwG_GG

Cuts at:      0     365    1095    1455 
   Size:        365     730     360

  Fragments arranged by size: 

                 730     365     360
 
PfoI T'CCnGG_A

Cuts at:      0     581     589    1259    1455 
   Size:        581       8     670     196

  Fragments arranged by size: 

                 670     581     196       8
 
PmlI CAC'GTG

Cuts at:      0     765    1455 
   Size:        765     690
 
PpuMI rG'GwC_Cy

Cuts at:      0     993    1126    1455 
   Size:        993     133     329

  Fragments arranged by size: 

                 993     329     133
 
PshAI GACnn'nnGTC

Cuts at:      0     481    1455 
   Size:        481     974
 
PspOMI G'GGCC_C

Cuts at:      0    1017    1042    1455 
   Size:       1017      25     413

  Fragments arranged by size: 

                1017     413      25
 
PstI C_TGCA'G

Cuts at:      0     839    1337    1455 
   Size:        839     498     118

  Fragments arranged by size: 

                 839     498     118
 
PvuII CAG'CTG

Cuts at:      0     405    1455 
   Size:        405    1050
 
RsrII CG'GwC_CG

Cuts at:      0     476    1455 
   Size:        476     979
 
SacI G_AGCT'C

Cuts at:      0    1313    1455 
   Size:       1313     142
 
SacII CC_GC'GG

Cuts at:      0     221    1455 
   Size:        221    1234
 
SanDI GG'GwC_CC

Cuts at:      0     993    1126    1455 
   Size:        993     133     329

  Fragments arranged by size: 

                 993     329     133
 
SfcI C'TryA_G

Cuts at:      0     835    1333    1455 
   Size:        835     498     122

  Fragments arranged by size: 

                 835     498     122
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1016    1455 
   Size:       1016     439
 
SfoI GGC'GCC

Cuts at:      0     650    1244    1271    1455 
   Size:        650     594      27     184

  Fragments arranged by size: 

                 650     594     184      27
 
SgrAI Cr'CCGG_yG

Cuts at:      0     416     730    1455 
   Size:        416     314     725

  Fragments arranged by size: 

                 725     416     314
 
SmaI CCC'GGG

Cuts at:      0     229     314     441     553     632     971    1370    1455 
   Size:        229      85     127     112      79     339     399      85

  Fragments arranged by size: 

                 399     339     229     127     112      85      85      79
            
 
SmlI C'TyrA_G

Cuts at:      0    1049    1455 
   Size:       1049     406
 
StuI AGG'CCT

Cuts at:      0      74    1384    1455 
   Size:         74    1310      71

  Fragments arranged by size: 

                1310      74      71
 
StyI C'CwwG_G

Cuts at:      0    1068    1455 
   Size:       1068     387
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1101    1455 
   Size:       1101     354
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     333    1455 
   Size:        333    1122
 
XmaI C'CCGG_G

Cuts at:      0     227     312     439     551     630     969    1368    1455 
   Size:        227      85     127     112      79     339     399      87

  Fragments arranged by size: 

                 399     339     227     127     112      87      85      79
            
 

 Enzymes that do cut:

     AarI     AlfI     AloI     ApaI    ApaLI     ApoI     AvaI    BamHI
     BanI    BanII     BbeI    BbvCI     BglI    BglII Bme1580I    BmgBI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstEII    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I     KasI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     PasI     PfoI
     PmlI    PpuMI    PshAI   PspOMI     PstI    PvuII    RsrII     SacI
    SacII    SanDI     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI
     StuI     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI    AlwNI     AscI     AseI    AsiSI    AvrII
     BaeI     BbsI     BcgI    BciVI     BclI    BfrBI     BlpI     BmrI
     BmtI     BsaI    BsaXI    BsiWI    BspHI    BsrDI    BsrGI   BstAPI
    BstBI    BstXI  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI     PpiI
     PsiI    PspXI     PsrI     PvuI     SalI     SapI     SbfI     ScaI
    SexAI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com