Restriction map for pF1KE1399
 (Linear) (Six-base) MAPSORT of: KIEE1399.seq  Check: 4646  from: 1  to: 5547



KIEE1399.orf

 With 182 enzymes: SgfI * 

                        July 25, 2014 11:38  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     956    2088    4594    5547 
   Size:        956    1132    2506     953

  Fragments arranged by size: 

                2506    1132     956     953
 
AatII G_ACGT'C

Cuts at:      0    4823    5547 
   Size:       4823     724
 
AccI GT'mk_AC

Cuts at:      0    2747    2999    5547 
   Size:       2747     252    2548

  Fragments arranged by size: 

                2747    2548     252
 
AclI AA'CG_TT

Cuts at:      0    4865    5547 
   Size:       4865     682
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     760    2635    2838    2909    5547 
   Size:        511     249    1875     203      71    2638

  Fragments arranged by size: 

                2638    1875     511     249     203      71
 
AfeI AGC'GCT

Cuts at:      0    1287    3411    5547 
   Size:       1287    2124    2136

  Fragments arranged by size: 

                2136    2124    1287
 
AflII C'TTAA_G

Cuts at:      0    2140    5547 
   Size:       2140    3407
 
AflIII A'CryG_T

Cuts at:      0    3911    5547 
   Size:       3911    1636
 
AleI CACnn'nnGTG

Cuts at:      0     169    1646    4105    5547 
   Size:        169    1477    2459    1442

  Fragments arranged by size: 

                2459    1477    1442     169
 
AlwNI CAG_nnn'CTG

Cuts at:      0     939    1347    1856    1992    2598    4288    5538    5547 
   Size:        939     408     509     136     606    1690    1250       9

  Fragments arranged by size: 

                1690    1250     939     606     509     408     136       9
            
 
ApaI G_GGCC'C

Cuts at:      0    1412    1553    3545    5547 
   Size:       1412     141    1992    2002

  Fragments arranged by size: 

                2002    1992    1412     141
 
ApaLI G'TGCA_C

Cuts at:      0    3946    5547 
   Size:       3946    1601
 
ApoI r'AATT_y

Cuts at:      0    1954    2778    3519    4246    5547 
   Size:       1954     824     741     727    1301

  Fragments arranged by size: 

                1954    1301     824     741     727
 
AvaI C'yCGr_G

Cuts at:      0     716     987    1241    1369    1405    1545    1579    2314 
   Size:        716     271     254     128      36     140      34     735

Cuts at:   2314    2519    3067    3379    3544    3592    3806    4093    4510 
   Size:        205     548     312     165      48     214     287     417

Cuts at:   4510    5492    5547 
   Size:        982      55

  Fragments arranged by size: 

                 982     735     716     548     417     312     287     271
                 254     214     205     165     140     128      55      48
                  36      34
 
BamHI G'GATC_C

Cuts at:      0     257     561    5547 
   Size:        257     304    4986

  Fragments arranged by size: 

                4986     304     257
 
BanI G'GyrC_C

Cuts at:      0     136    1510    2217    3478    4041    5047    5547 
   Size:        136    1374     707    1261     563    1006     500

  Fragments arranged by size: 

                1374    1261    1006     707     563     500     136
 
BanII G_rGCy'C

Cuts at:      0     666    1412    1553    1828    2276    2428    2494    3458 
   Size:        666     746     141     275     448     152      66     964

Cuts at:   3458    3545    4100    4138    5547 
   Size:         87     555      38    1409

  Fragments arranged by size: 

                1409     964     746     666     555     448     275     152
                 141      87      66      38
 
BbeI G_GCGC'C

Cuts at:      0    3482    5547 
   Size:       3482    2065
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     487     748    1390    1879    2897    3079    4550    5547 
   Size:        487     261     642     489    1018     182    1471     997

  Fragments arranged by size: 

                1471    1018     997     642     489     487     261     182
            
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3144    3177    3178    3211    3472    3506    4104    4138 
   Size:       3144      33       1      33     261      34     598      34

Cuts at:   4138    5547 
   Size:       1409

  Fragments arranged by size: 

                3144    1409     598     261      34      34      33      33
                   1
 
BfrBI ATG'CAT

Cuts at:      0     212     500    5547 
   Size:        212     288    5047

  Fragments arranged by size: 

                5047     288     212
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1482    1584    3021    3718    5547 
   Size:       1482     102    1437     697    1829

  Fragments arranged by size: 

                1829    1482    1437     697     102
 
BglII A'GATC_T

Cuts at:      0    4079    5547 
   Size:       4079    1468
 
BlpI GC'TnA_GC

Cuts at:      0     614    3413    5547 
   Size:        614    2799    2134

  Fragments arranged by size: 

                2799    2134     614
 
Bme1580I G_kGCm'C

Cuts at:      0     929     978    1178    1406    1412    1553    1580    2220 
   Size:        929      49     200     228       6     141      27     640

Cuts at:   2220    2684    3545    3566    3950    4112    4767    5121    5504 
   Size:        464     861      21     384     162     655     354     383

Cuts at:   5504    5547 
   Size:         43

  Fragments arranged by size: 

                 929     861     655     640     464     384     383     354
                 228     200     162     141      49      43      27      21
                   6
 
BmgBI CAC'GTC

Cuts at:      0    3077    3527    3914    5116    5547 
   Size:       3077     450     387    1202     431

  Fragments arranged by size: 

                3077    1202     450     431     387
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1305    4627    5547 
   Size:       1305    3322     920

  Fragments arranged by size: 

                3322    1305     920
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     961    2014    2287    4341    4774    5547 
   Size:        427     534    1053     273    2054     433     773

  Fragments arranged by size: 

                2054    1053     773     534     433     427     273
 
Bpu10I CC'TnA_GC

Cuts at:      0     606    4643    5547 
   Size:        606    4037     904

  Fragments arranged by size: 

                4037     904     606
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      85     577     586    3841    3857    5139    5152    5547 
   Size:         85     492       9    3255      16    1282      13     395

  Fragments arranged by size: 

                3255    1282     492     395      85      16      13       9
            
 
BsaAI yAC'GTr

Cuts at:      0     930    1401    5547 
   Size:        930     471    4146

  Fragments arranged by size: 

                4146     930     471
 
BsaBI GATnn'nnATC

Cuts at:      0    3901    5547 
   Size:       3901    1646
 
BsaHI Gr'CG_yC

Cuts at:      0    2473    3479    3959    4451    4820    5547 
   Size:       2473    1006     480     492     369     727

  Fragments arranged by size: 

                2473    1006     727     492     480     369
 
BsaWI w'CCGG_w

Cuts at:      0     586    2294    3866    5547 
   Size:        586    1708    1572    1681

  Fragments arranged by size: 

                1708    1681    1572     586
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1735    1765    2860    2890    3826    3856    4562    4592 
   Size:       1735      30    1095      30     936      30     706      30

Cuts at:   4592    5178    5208    5547 
   Size:        586      30     339

  Fragments arranged by size: 

                1735    1095     936     706     586     339      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     163     367    1756    1882    2284    2332    2594    3397 
   Size:        163     204    1389     126     402      48     262     803

Cuts at:   3397    4166    4226    4489    5547 
   Size:        769      60     263    1058

  Fragments arranged by size: 

                1389    1058     803     769     402     263     262     204
                 163     126      60      48
 
BseYI C'CCAG_C

Cuts at:      0    1340    1360    1440    1977    2401    3505    3555    3634 
   Size:       1340      20      80     537     424    1104      50      79

Cuts at:   3634    3763    3917    4766    5054    5268    5547 
   Size:        129     154     849     288     214     279

  Fragments arranged by size: 

                1340    1104     849     537     424     288     279     214
                 154     129      80      79      50      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838     952    4572    5547 
   Size:        838     114    3620     975

  Fragments arranged by size: 

                3620     975     838     114
 
BsiEI CG_ry'CG

Cuts at:      0    3264    5547 
   Size:       3264    2283
 
BsiHKAI G_wGCw'C

Cuts at:      0     666     947    2276    3082    3495    3950    4999    5020 
   Size:        666     281    1329     806     413     455    1049      21

Cuts at:   5020    5547 
   Size:        527

  Fragments arranged by size: 

                1329    1049     806     666     527     455     413     281
                  21
 
BsmI GAATG_Cn'

Cuts at:      0     212     987    2913    5547 
   Size:        212     775    1926    2634

  Fragments arranged by size: 

                2634    1926     775     212
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3823    4507    5547 
   Size:       3823     684    1040

  Fragments arranged by size: 

                3823    1040     684
 
Bsp1286I G_dGCh'C

Cuts at:      0     666     929     947     978    1178    1406    1412    1553 
   Size:        666     263      18      31     200     228       6     141

Cuts at:   1553    1580    1828    2220    2276    2428    2494    2684    3082 
   Size:         27     248     392      56     152      66     190     398

Cuts at:   3082    3458    3495    3545    3566    3950    4100    4112    4138 
   Size:        376      37      50      21     384     150      12      26

Cuts at:   4138    4767    4999    5020    5121    5504    5547 
   Size:        629     232      21     101     383      43

  Fragments arranged by size: 

                 666     629     398     392     384     383     376     263
                 248     232     228     200     190     152     150     141
                 101      66      56      50      43      37      31      27
                  26      21      21      18      12       6
 
BspEI T'CCGG_A

Cuts at:      0    2294    5547 
   Size:       2294    3253
 
BspHI T'CATG_A

Cuts at:      0    4991    5547 
   Size:       4991     556
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     203     908     956    2088    4594    5465    5547 
   Size:        203     705      48    1132    2506     871      82

  Fragments arranged by size: 

                2506    1132     871     705     203      82      48
 
BsrBI CCG'CTC

Cuts at:      0     357     613    2589    4741    5206    5547 
   Size:        357     256    1976    2152     465     341

  Fragments arranged by size: 

                2152    1976     465     357     341     256
 
BsrDI GCAATG_nn'

Cuts at:      0     330    4228    4683    5547 
   Size:        330    3898     455     864

  Fragments arranged by size: 

                3898     864     455     330
 
BsrFI r'CCGG_y

Cuts at:      0     315    1208    1418    1968    2408    2911    3586    3775 
   Size:        315     893     210     550     440     503     675     189

Cuts at:   3775    5075    5547 
   Size:       1300     472

  Fragments arranged by size: 

                1300     893     675     550     503     472     440     315
                 210     189
 
BsrGI T'GTAC_A

Cuts at:      0     265    5547 
   Size:        265    5282
 
BssSI C'ACGA_G

Cuts at:      0     898    2275    4699    5547 
   Size:        898    1377    2424     848

  Fragments arranged by size: 

                2424    1377     898     848
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1356    2411    2679    5547 
   Size:       1356    1055     268    2868

  Fragments arranged by size: 

                2868    1356    1055     268
 
BstEII G'GTnAC_C

Cuts at:      0    2154    3789    5025    5547 
   Size:       2154    1635    1236     522

  Fragments arranged by size: 

                2154    1635    1236     522
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     546    1230    2166    3318    3969    5547 
   Size:        546     684     936    1152     651    1578

  Fragments arranged by size: 

                1578    1152     936     684     651     546
 
BstYI r'GATC_y

Cuts at:      0     257     561     902    1631    4079    5250    5547 
   Size:        257     304     341     729    2448    1171     297

  Fragments arranged by size: 

                2448    1171     729     341     304     297     257
 
Bsu36I CC'TnA_GG

Cuts at:      0    3817    5103    5547 
   Size:       3817    1286     444

  Fragments arranged by size: 

                3817    1286     444
 
BtgI C'CryG_G

Cuts at:      0      59     383    1310    1644    1704    1820    2000    2819 
   Size:         59     324     927     334      60     116     180     819

Cuts at:   2819    3953    4139    4193    5547 
   Size:       1134     186      54    1354

  Fragments arranged by size: 

                1354    1134     927     819     334     324     186     180
                 116      60      59      54
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     502    1412    3228    3890    5547 
   Size:        502     910    1816     662    1657

  Fragments arranged by size: 

                1816    1657     910     662     502
 
BtsI GCAGTG_nn'

Cuts at:      0     116    3566    4767    5547 
   Size:        116    3450    1201     780

  Fragments arranged by size: 

                3450    1201     780     116
 
ClaI AT'CG_AT

Cuts at:      0    5435    5477    5547 
   Size:       5435      42      70

  Fragments arranged by size: 

                5435      70      42
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     639     674    5111    5146    5547 
   Size:        639      35    4437      35     401

  Fragments arranged by size: 

                4437     639     401      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     239     872    3954    5547 
   Size:        239     633    3082    1593

  Fragments arranged by size: 

                3082    1593     633     239
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1300    5547 
   Size:       1300    4247
 
EaeI y'GGCC_r

Cuts at:      0     317     464    1109    1232    1313    1707    2088    2822 
   Size:        317     147     645     123      81     394     381     734

Cuts at:   2822    4778    5372    5547 
   Size:       1956     594     175

  Fragments arranged by size: 

                1956     734     645     594     394     381     317     175
                 147     123      81
 
EarI CTCTTCn'nnn_

Cuts at:      0      83    1594    2579    3983    4139    5547 
   Size:         83    1511     985    1404     156    1408

  Fragments arranged by size: 

                1511    1408    1404     985     156      83
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1076    1744    3039    4950    5547 
   Size:       1076     668    1295    1911     597

  Fragments arranged by size: 

                1911    1295    1076     668     597
 
EcoICRI GAG'CTC

Cuts at:      0     664    2274    5547 
   Size:        664    1610    3273

  Fragments arranged by size: 

                3273    1610     664
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     511     760     961    2014    2287    2635    2838 
   Size:        427      84     249     201    1053     273     348     203

Cuts at:   2838    2909    4341    4774    5547 
   Size:         71    1432     433     773

  Fragments arranged by size: 

                1432    1053     773     433     427     348     273     249
                 203     201      84      71
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     349    5461    5547 
   Size:        349    5112      86

  Fragments arranged by size: 

                5112     349      86
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1188    1245    1409    1549    3174    3541    3697    3711 
   Size:       1188      57     164     140    1625     367     156      14

Cuts at:   3711    3811    4799    5099    5469    5547 
   Size:        100     988     300     370      78

  Fragments arranged by size: 

                1625    1188     988     370     367     300     164     156
                 140     100      78      57      14
 
EcoRI G'AATT_C

Cuts at:      0    1954    2778    3519    5547 
   Size:       1954     824     741    2028

  Fragments arranged by size: 

                2028    1954     824     741
 
EcoRV GAT'ATC

Cuts at:      0    4266    5547 
   Size:       4266    1281
 
HaeII r_GCGC'y

Cuts at:      0    1289    1703    3413    3482    5547 
   Size:       1289     414    1710      69    2065

  Fragments arranged by size: 

                2065    1710    1289     414      69
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1513    1545    2148    2180    2272    2304    2422    2454 
   Size:       1513      32     603      32      92      32     118      32

Cuts at:   2454    2860    2892    3406    3438    3622    3654    5547 
   Size:        406      32     514      32     184      32    1893

  Fragments arranged by size: 

                1893    1513     603     514     406     184     118      92
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2064    4623    5547 
   Size:       2064    2559     924

  Fragments arranged by size: 

                2559    2064     924
 
HindIII A'AGCT_T

Cuts at:      0    1801    4443    5547 
   Size:       1801    2642    1104

  Fragments arranged by size: 

                2642    1801    1104
 
HpaI GTT'AAC

Cuts at:      0    2064    5547 
   Size:       2064    3483
 
KasI G'GCGC_C

Cuts at:      0    3478    5547 
   Size:       3478    2069
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      47      74     801    1226    1283    2837    2884    3074 
   Size:         47      27     727     425      57    1554      47     190

Cuts at:   3074    5547 
   Size:       2473

  Fragments arranged by size: 

                2473    1554     727     425     190      57      47      47
                  27
 
MscI TGG'CCA

Cuts at:      0     466    1111    2824    4780    5547 
   Size:        466     645    1713    1956     767

  Fragments arranged by size: 

                1956    1713     767     645     466
 
MslI CAynn'nnrTG

Cuts at:      0     169     541    1646    2365    2830    3103    4105    5547 
   Size:        169     372    1105     719     465     273    1002    1442

  Fragments arranged by size: 

                1442    1105    1002     719     465     372     273     169
            
 
MspA1I CmG'CkG

Cuts at:      0     255     618     801    1034    1344    1646    1706    2397 
   Size:        255     363     183     233     310     302      60     691

Cuts at:   2397    3131    3195    3420    3516    3578    3645    4087    5272 
   Size:        734      64     225      96      62      67     442    1185

Cuts at:   5272    5535    5547 
   Size:        263      12

  Fragments arranged by size: 

                1185     734     691     442     363     310     302     263
                 255     233     225     183      96      67      64      62
                  60      12
 
NaeI GCC'GGC

Cuts at:      0     317    1210    2913    3588    3777    5077    5547 
   Size:        317     893    1703     675     189    1300     470

  Fragments arranged by size: 

                1703    1300     893     675     470     317     189
 
NarI GG'CG_CC

Cuts at:      0    3479    5547 
   Size:       3479    2068
 
NcoI C'CATG_G

Cuts at:      0    4139    5547 
   Size:       4139    1408
 
NgoMIV G'CCGG_C

Cuts at:      0     315    1208    2911    3586    3775    5075    5547 
   Size:        315     893    1703     675     189    1300     472

  Fragments arranged by size: 

                1703    1300     893     675     472     315     189
 
NsiI A_TGCA'T

Cuts at:      0     214     502    5547 
   Size:        214     288    5045

  Fragments arranged by size: 

                5045     288     214
 
NspI r_CATG'y

Cuts at:      0    1489    3894    5547 
   Size:       1489    2405    1653

  Fragments arranged by size: 

                2405    1653    1489
 
PasI CC'CwG_GG

Cuts at:      0    1760    3449    3537    5547 
   Size:       1760    1689      88    2010

  Fragments arranged by size: 

                2010    1760    1689      88
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     239    3471    4811    5547 
   Size:        239    3232    1340     736

  Fragments arranged by size: 

                3232    1340     736     239
 
PfoI T'CCnGG_A

Cuts at:      0     344    5547 
   Size:        344    5203
 
PmlI CAC'GTG

Cuts at:      0     930    5547 
   Size:        930    4617
 
PpuMI rG'GwC_Cy

Cuts at:      0    1245    3697    3811    4799    5099    5469    5547 
   Size:       1245    2452     114     988     300     370      78

  Fragments arranged by size: 

                2452    1245     988     370     300     114      78
 
PshAI GACnn'nnGTC

Cuts at:      0    1879    5547 
   Size:       1879    3668
 
PsiI TTA'TAA

Cuts at:      0     776    5547 
   Size:        776    4771
 
PspOMI G'GGCC_C

Cuts at:      0    1408    1549    3541    5547 
   Size:       1408     141    1992    2006

  Fragments arranged by size: 

                2006    1992    1408     141
 
PspXI vC'TCGA_Gb

Cuts at:      0    2519    5492    5547 
   Size:       2519    2973      55

  Fragments arranged by size: 

                2973    2519      55
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2428    2460    5547 
   Size:       2428      32    3087

  Fragments arranged by size: 

                3087    2428      32
 
PstI C_TGCA'G

Cuts at:      0     200     919    1352    1889    3986    4380    5072    5462 
   Size:        200     719     433     537    2097     394     692     390

Cuts at:   5462    5547 
   Size:         85

  Fragments arranged by size: 

                2097     719     692     537     433     394     390     200
                  85
 
PvuII CAG'CTG

Cuts at:      0     618    1034    1344    3195    3420    5535    5547 
   Size:        618     416     310    1851     225    2115      12

  Fragments arranged by size: 

                2115    1851     618     416     310     225      12
 
RsrII CG'GwC_CG

Cuts at:      0    2292    5547 
   Size:       2292    3255
 
SacI G_AGCT'C

Cuts at:      0     666    2276    5547 
   Size:        666    1610    3271

  Fragments arranged by size: 

                3271    1610     666
 
SacII CC_GC'GG

Cuts at:      0    1647    1707    5547 
   Size:       1647      60    3840

  Fragments arranged by size: 

                3840    1647      60
 
SanDI GG'GwC_CC

Cuts at:      0    3697    3811    5547 
   Size:       3697     114    1736

  Fragments arranged by size: 

                3697    1736     114
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1594    2579    5547 
   Size:       1594     985    2968

  Fragments arranged by size: 

                2968    1594     985
 
SbfI CC_TGCA'GG

Cuts at:      0     919    3986    5547 
   Size:        919    3067    1561

  Fragments arranged by size: 

                3067    1561     919
 
SexAI A'CCwGG_T

Cuts at:      0    2132    2806    5547 
   Size:       2132     674    2741

  Fragments arranged by size: 

                2741    2132     674
 
SfcI C'TryA_G

Cuts at:      0     196     915    1348    1566    1885    1984    2898    3982 
   Size:        196     719     433     218     319      99     914    1084

Cuts at:   3982    4376    4891    5068    5458    5547 
   Size:        394     515     177     390      89

  Fragments arranged by size: 

                1084     914     719     515     433     394     390     319
                 218     196     177      99      89
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3718    5547 
   Size:       3718    1829
 
SfoI GGC'GCC

Cuts at:      0    3480    5547 
   Size:       3480    2067
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1208    5547 
   Size:       1208    4339
 
SmaI CCC'GGG

Cuts at:      0    1243    1407    1547    1581    3594    3808    5547 
   Size:       1243     164     140      34    2013     214    1739

  Fragments arranged by size: 

                2013    1739    1243     214     164     140      34
 
SmlI C'TyrA_G

Cuts at:      0    2140    2519    4510    5492    5547 
   Size:       2140     379    1991     982      55

  Fragments arranged by size: 

                2140    1991     982     379      55
 
SrfI GCCC'GGGC

Cuts at:      0    1407    5547 
   Size:       1407    4140
 
StuI AGG'CCT

Cuts at:      0     557     913    5416    5547 
   Size:        557     356    4503     131

  Fragments arranged by size: 

                4503     557     356     131
 
StyI C'CwwG_G

Cuts at:      0    2454    2789    2879    3716    4139    4781    4795    5547 
   Size:       2454     335      90     837     423     642      14     752

  Fragments arranged by size: 

                2454     837     752     642     423     335      90      14
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     376     566    1959    2280    3463    3623    3686    3865 
   Size:        376     190    1393     321    1183     160      63     179

Cuts at:   3865    5389    5547 
   Size:       1524     158

  Fragments arranged by size: 

                1524    1393    1183     376     321     190     179     160
                 158      63
 
TatI w'GTAC_w

Cuts at:      0     265    2849    5547 
   Size:        265    2584    2698

  Fragments arranged by size: 

                2698    2584     265
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1735    1767    1958    1990    2450    2482    4562    4594 
   Size:       1735      32     191      32     460      32    2080      32

Cuts at:   4594    5178    5210    5547 
   Size:        584      32     337

  Fragments arranged by size: 

                2080    1735     584     460     337     191      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1654    4453    5547 
   Size:       1654    2799    1094

  Fragments arranged by size: 

                2799    1654    1094
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3472    3642    4128    5242    5547 
   Size:       3472     170     486    1114     305

  Fragments arranged by size: 

                3472    1114     486     305     170
 
XhoI C'TCGA_G

Cuts at:      0    2519    4510    5492    5547 
   Size:       2519    1991     982      55

  Fragments arranged by size: 

                2519    1991     982      55
 
XmaI C'CCGG_G

Cuts at:      0    1241    1405    1545    1579    3592    3806    5547 
   Size:       1241     164     140      34    2013     214    1741

  Fragments arranged by size: 

                2013    1741    1241     214     164     140      34
 
XmnI GAAnn'nnTTC

Cuts at:      0    1804    5547 
   Size:       1804    3743
 
ZraI GAC'GTC

Cuts at:      0    4821    5547 
   Size:       4821     726
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AleI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI    PvuII    RsrII     SacI    SacII    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI    SgrAI     SmaI
     SmlI     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AgeI     AhdI     AlfI     AloI     AscI     AseI    AsiSI
    AvrII     BaeI    BbvCI    BciVI     BclI     BmtI     BplI    BpuEI
    BsiWI   BssHII    BstBI  BstZ17I     DraI     EagI     FalI     FseI
     FspI    FspAI     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     PacI     PciI     PmeI     PpiI     PvuI     SalI     ScaI
     SgfI    SnaBI     SpeI     SphI     SspI     SwaI     XbaI
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