(Circular) (Six-base) MAPSORT of: pF1KE3547.seq Check: 7250 from: 1 to: 9302 pF1KE3547 9302 bp With 182 enzymes: SgfI * July 9, 2014 09:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1627 1759 3834 1627 Size: 132 2075 7095 Fragments arranged by size: 7095 2075 132 AatII G_ACGT'C Cuts at: 3125 4871 5804 3125 Size: 1746 933 6623 Fragments arranged by size: 6623 1746 933 AccI GT'mk_AC Cuts at: 1476 5303 5492 5798 6340 1476 Size: 3827 189 306 542 4438 Fragments arranged by size: 4438 3827 542 306 189 Acc65I G'GTAC_C Cuts at: 2095 6318 2095 Size: 4223 5079 AclI AA'CG_TT Cuts at: 9142 9142 Size: 9302 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1819 2234 2369 3415 3799 4087 4582 7127 7559 Size: 415 135 1046 384 288 495 2545 432 Cuts at: 7559 8306 8574 1819 Size: 747 268 2547 Fragments arranged by size: 2547 2545 1046 747 495 432 415 384 288 268 135 AfeI AGC'GCT Cuts at: 169 5885 169 Size: 5716 3586 AflIII A'CryG_T Cuts at: 2973 5360 5660 5753 6654 7758 8735 2973 Size: 2387 300 93 901 1104 977 3540 Fragments arranged by size: 3540 2387 1104 977 901 300 93 AgeI A'CCGG_T Cuts at: 4022 7690 4022 Size: 3668 5634 AhdI GACnn_n'nnGTC Cuts at: 1320 2239 2450 2844 5031 6254 1320 Size: 919 211 394 2187 1223 4368 Fragments arranged by size: 4368 2187 1223 919 394 211 AleI CACnn'nnGTG Cuts at: 3155 3155 Size: 9302 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2100 3388 4612 5879 2100 Size: 1288 1224 1267 5523 Fragments arranged by size: 5523 1288 1267 1224 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3136 3168 3136 Size: 32 9270 AlwNI CAG_nnn'CTG Cuts at: 1834 2023 2062 2257 2462 3393 3711 4317 4380 Size: 189 39 195 205 931 318 606 63 Cuts at: 4380 6607 8174 1834 Size: 2227 1567 2962 Fragments arranged by size: 2962 2227 1567 931 606 318 205 195 189 63 39 ApaI G_GGCC'C Cuts at: 302 460 789 880 2187 2641 2772 2958 302 Size: 158 329 91 1307 454 131 186 6646 Fragments arranged by size: 6646 1307 454 329 186 158 131 91 ApaLI G'TGCA_C Cuts at: 5815 8072 5815 Size: 2257 7045 ApoI r'AATT_y Cuts at: 6306 8655 8911 6306 Size: 2349 256 6697 Fragments arranged by size: 6697 2349 256 AseI AT'TA_AT Cuts at: 20 20 Size: 9302 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 9302 AvaI C'yCGr_G Cuts at: 184 257 410 593 788 930 985 1988 2850 Size: 73 153 183 195 142 55 1003 862 Cuts at: 2850 2951 3083 3115 3460 4435 4777 4950 5049 Size: 101 132 32 345 975 342 173 99 Cuts at: 5049 5115 5273 6106 6322 184 Size: 66 158 833 216 3164 Fragments arranged by size: 3164 1003 975 862 833 345 342 216 195 183 173 158 153 142 132 101 99 73 66 55 32 BamHI G'GATC_C Cuts at: 1848 3743 6327 1848 Size: 1895 2584 4823 Fragments arranged by size: 4823 2584 1895 BanI G'GyrC_C Cuts at: 120 571 1429 1837 1924 2095 2218 2530 2737 Size: 451 858 408 87 171 123 312 207 Cuts at: 2737 3018 3105 4441 4773 4839 5004 5218 5551 Size: 281 87 1336 332 66 165 214 333 Cuts at: 5551 5917 5979 6146 6318 6732 6980 7015 120 Size: 366 62 167 172 414 248 35 2407 Fragments arranged by size: 2407 1336 858 451 414 408 366 333 332 312 281 248 214 207 172 171 167 165 123 87 87 66 62 35 BanII G_rGCy'C Cuts at: 302 460 789 880 900 992 1087 1094 1113 Size: 158 329 91 20 92 95 7 19 Cuts at: 1113 1388 1588 1820 2015 2187 2392 2401 2414 Size: 275 200 232 195 172 205 9 13 Cuts at: 2414 2641 2654 2672 2699 2772 2958 3090 3467 Size: 227 13 18 27 73 186 132 377 Cuts at: 3467 3546 4643 4850 5244 5326 5566 5638 6316 Size: 79 1097 207 394 82 240 72 678 Cuts at: 6316 7346 302 Size: 1030 2258 Fragments arranged by size: 2258 1097 1030 678 394 377 329 275 240 232 227 207 205 200 195 186 172 158 132 95 92 91 82 79 73 72 27 20 19 18 13 13 9 7 BbeI G_GCGC'C Cuts at: 6736 6984 6736 Size: 248 9054 BbsI GAAGACnn'nnnn_ Cuts at: 4181 5027 4181 Size: 846 8456 BbvCI CC'TCA_GC Cuts at: 190 1561 2324 2424 3221 4819 190 Size: 1371 763 100 797 1598 4673 Fragments arranged by size: 4673 1598 1371 797 763 100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 486 520 2542 2576 4861 4895 486 Size: 34 2022 34 2285 34 4893 Fragments arranged by size: 4893 2285 2022 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7200 7961 9302 7200 Size: 761 1341 7200 Fragments arranged by size: 7200 1341 761 BclI T'GATC_A Cuts at: 3725 6822 3725 Size: 3097 6205 BglI GCCn_nnn'nGGC Cuts at: 273 1262 3646 3886 4854 273 Size: 989 2384 240 968 4721 Fragments arranged by size: 4721 2384 989 968 240 BglII A'GATC_T Cuts at: 3443 6817 8854 3443 Size: 3374 2037 3891 Fragments arranged by size: 3891 3374 2037 BlpI GC'TnA_GC Cuts at: 3851 4699 6414 3851 Size: 848 1715 6739 Fragments arranged by size: 6739 1715 848 Bme1580I G_kGCm'C Cuts at: 123 302 460 789 880 1929 2187 2223 2641 Size: 179 158 329 91 1049 258 36 418 Cuts at: 2641 2757 2772 2958 2996 4444 4778 5009 5341 Size: 116 15 186 38 1448 334 231 332 Cuts at: 5341 5554 5751 5819 5920 5982 6927 7020 8076 Size: 213 197 68 101 62 945 93 1056 Cuts at: 8076 123 Size: 1349 Fragments arranged by size: 1448 1349 1056 1049 945 418 334 332 329 258 231 213 197 186 179 158 116 101 93 91 68 62 38 36 15 BmgBI CAC'GTC Cuts at: 4722 6024 4722 Size: 1302 8000 BmrI ACTGGGnnnn_n' Cuts at: 1980 2369 3166 6514 6595 6930 9074 1980 Size: 389 797 3348 81 335 2144 2208 Fragments arranged by size: 3348 2208 2144 797 389 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2031 3565 4844 7669 2031 Size: 1534 1279 2825 3664 Fragments arranged by size: 3664 2825 1534 1279 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 184 192 504 840 914 1445 2663 2952 3490 Size: 8 312 336 74 531 1218 289 538 Cuts at: 3490 3511 3841 3934 4435 4814 5076 5238 5395 Size: 21 330 93 501 379 262 162 157 Cuts at: 5395 5674 5697 6145 184 Size: 279 23 448 3341 Fragments arranged by size: 3341 1218 538 531 501 448 379 336 330 312 289 279 262 162 157 93 74 23 21 8 Bpu10I CC'TnA_GC Cuts at: 190 991 1088 1561 2063 2324 2424 3023 3221 Size: 801 97 473 502 261 100 599 198 Cuts at: 3221 4819 5632 190 Size: 1598 813 3860 Fragments arranged by size: 3860 1598 813 801 599 502 473 261 198 100 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 240 1288 1693 3649 5305 6478 7357 7849 8147 Size: 1048 405 1956 1656 1173 879 492 298 Cuts at: 8147 240 Size: 1395 Fragments arranged by size: 1956 1656 1395 1173 1048 879 492 405 298 BsaI GGTCTCn'nnnn_ Cuts at: 676 1291 1510 2194 4031 5104 5134 9006 676 Size: 615 219 684 1837 1073 30 3872 972 Fragments arranged by size: 3872 1837 1073 972 684 615 219 30 BsaAI yAC'GTr Cuts at: 3315 4680 5361 5670 5754 6655 3315 Size: 1365 681 309 84 901 5962 Fragments arranged by size: 5962 1365 901 681 309 84 BsaBI GATnn'nnATC Cuts at: 6 6816 8853 6 Size: 6810 2037 455 Fragments arranged by size: 6810 2037 455 BsaHI Gr'CG_yC Cuts at: 292 422 616 3122 4478 4868 4966 5801 6733 Size: 130 194 2506 1356 390 98 835 932 Cuts at: 6733 6981 7683 9179 292 Size: 248 702 1496 415 Fragments arranged by size: 2506 1496 1356 932 835 702 415 390 248 194 130 98 BsaWI w'CCGG_w Cuts at: 3154 4022 4842 6491 7012 7690 7964 8111 8522 Size: 868 820 1649 521 678 274 147 411 Cuts at: 8522 3154 Size: 3934 Fragments arranged by size: 3934 1649 868 820 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 382 412 3136 3166 5308 5338 5356 5386 5471 Size: 30 2724 30 2142 30 18 30 85 Cuts at: 5471 5501 5647 5677 5893 5923 6121 6151 382 Size: 30 146 30 216 30 198 30 3533 Fragments arranged by size: 3533 2724 2142 216 198 146 85 30 30 30 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 211 482 792 795 798 877 886 918 1223 Size: 271 310 3 3 79 9 32 305 Cuts at: 1223 1800 1858 2023 2955 3898 4799 4836 5079 Size: 577 58 165 932 943 901 37 243 Cuts at: 5079 5122 5170 5173 5798 5914 6271 211 Size: 43 48 3 625 116 357 3242 Fragments arranged by size: 3242 943 932 901 625 577 357 310 305 271 243 165 116 79 58 48 43 37 32 9 3 3 3 BseYI C'CCAG_C Cuts at: 131 403 459 478 529 559 971 1343 1375 Size: 272 56 19 51 30 412 372 32 Cuts at: 1375 1674 1680 2599 2620 2883 3527 5041 5334 Size: 299 6 919 21 263 644 1514 293 Cuts at: 5334 6727 7485 8062 8558 131 Size: 1393 758 577 496 875 Fragments arranged by size: 1514 1393 919 875 758 644 577 496 412 372 299 293 272 263 56 51 32 30 21 19 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1903 3133 3191 3757 4041 1903 Size: 1230 58 566 284 7164 Fragments arranged by size: 7164 1230 566 284 58 BsiEI CG_ry'CG Cuts at: 104 244 437 452 706 948 4007 6844 6890 Size: 140 193 15 254 242 3059 2837 46 Cuts at: 6890 8098 104 Size: 1208 1308 Fragments arranged by size: 3059 2837 1308 1208 254 242 193 140 46 15 BsiHKAI G_wGCw'C Cuts at: 1588 1797 1820 2015 3419 3467 3476 5326 5660 Size: 209 23 195 1404 48 9 1850 334 Cuts at: 5660 5819 6131 6316 7094 7284 8076 1588 Size: 159 312 185 778 190 792 2814 Fragments arranged by size: 2814 1850 1404 792 778 334 312 209 195 190 185 159 48 23 9 BsmBI CGTCTCn'nnnn_ Cuts at: 153 407 944 2909 4828 6254 153 Size: 254 537 1965 1919 1426 3201 Fragments arranged by size: 3201 1965 1919 1426 537 254 Bsp1286I G_dGCh'C Cuts at: 123 302 460 789 880 900 992 1087 1094 Size: 179 158 329 91 20 92 95 7 Cuts at: 1094 1113 1388 1588 1797 1820 1929 2015 2187 Size: 19 275 200 209 23 109 86 172 Cuts at: 2187 2223 2392 2401 2414 2641 2654 2672 2699 Size: 36 169 9 13 227 13 18 27 Cuts at: 2699 2757 2772 2958 2996 3090 3419 3467 3476 Size: 58 15 186 38 94 329 48 9 Cuts at: 3476 3546 4444 4643 4778 4850 5009 5244 5326 Size: 70 898 199 135 72 159 235 82 Cuts at: 5326 5341 5554 5566 5638 5660 5751 5819 5920 Size: 15 213 12 72 22 91 68 101 Cuts at: 5920 5982 6131 6316 6927 7020 7094 7284 7346 Size: 62 149 185 611 93 74 190 62 Cuts at: 7346 8076 123 Size: 730 1349 Fragments arranged by size: 1349 898 730 611 329 329 275 235 227 213 209 200 199 190 186 185 179 172 169 159 158 149 135 109 101 95 94 93 92 91 91 86 82 74 72 72 70 68 62 62 58 48 38 36 27 23 22 20 19 18 15 15 13 13 12 9 9 7 BspEI T'CCGG_A Cuts at: 8522 8522 Size: 9302 BspHI T'CATG_A Cuts at: 3722 4577 8478 3722 Size: 855 3901 4546 Fragments arranged by size: 4546 3901 855 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1627 1759 3155 3486 3834 5300 5440 6074 6352 Size: 132 1396 331 348 1466 140 634 278 Cuts at: 6352 6566 6868 7249 1627 Size: 214 302 381 3680 Fragments arranged by size: 3680 1466 1396 634 381 348 331 302 278 214 140 132 BsrBI CCG'CTC Cuts at: 50 176 330 723 1358 5252 7594 7648 9132 Size: 126 154 393 635 3894 2342 54 1484 Cuts at: 9132 9297 50 Size: 165 55 Fragments arranged by size: 3894 2342 1484 635 393 165 154 126 55 54 BsrDI GCAATG_nn' Cuts at: 2283 4940 5109 7214 2283 Size: 2657 169 2105 4371 Fragments arranged by size: 4371 2657 2105 169 BsrFI r'CCGG_y Cuts at: 239 701 4022 5075 6016 7300 7481 7690 239 Size: 462 3321 1053 941 1284 181 209 1851 Fragments arranged by size: 3321 1851 1284 1053 941 462 209 181 BsrGI T'GTAC_A Cuts at: 4109 4109 Size: 9302 BssHII G'CGCG_C Cuts at: 4707 4707 Size: 9302 BssSI C'ACGA_G Cuts at: 234 3478 7573 7931 234 Size: 3244 4095 358 1605 Fragments arranged by size: 4095 3244 1605 358 BstAPI GCAn_nnn'nTGC Cuts at: 3138 3281 3510 3625 3684 4760 5823 6679 3138 Size: 143 229 115 59 1076 1063 856 5761 Fragments arranged by size: 5761 1076 1063 856 229 143 115 59 BstBI TT'CG_AA Cuts at: 7663 7663 Size: 9302 BstEII G'GTnAC_C Cuts at: 4984 4984 Size: 9302 BstXI CCAn_nnnn'nTGG Cuts at: 2155 5229 2155 Size: 3074 6228 BstYI r'GATC_y Cuts at: 1 1848 1910 3443 3455 3743 3945 6327 6794 Size: 1847 62 1533 12 288 202 2382 467 Cuts at: 6794 6817 7152 7398 8410 8496 8508 8854 1 Size: 23 335 246 1012 86 12 346 449 Fragments arranged by size: 2382 1847 1533 1012 467 449 346 335 288 246 202 86 62 23 12 12 Bsu36I CC'TnA_GG Cuts at: 2777 3236 3791 4649 5309 2777 Size: 459 555 858 660 6770 Fragments arranged by size: 6770 858 660 555 459 BtgI C'CryG_G Cuts at: 107 248 431 437 915 1269 1596 1766 2148 Size: 141 183 6 478 354 327 170 382 Cuts at: 2148 2429 2607 5132 5259 5974 107 Size: 281 178 2525 127 715 3435 Fragments arranged by size: 3435 2525 715 478 382 354 327 281 183 178 170 141 127 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 798 1961 3095 4719 7252 7433 7596 798 Size: 1163 1134 1624 2533 181 163 2504 Fragments arranged by size: 2533 2504 1624 1163 1134 181 163 BtsI GCAGTG_nn' Cuts at: 942 2481 2798 3504 4327 4596 6231 6249 6545 Size: 1539 317 706 823 269 1635 18 296 Cuts at: 6545 942 Size: 3699 Fragments arranged by size: 3699 1635 1539 823 706 317 296 269 18 ClaI AT'CG_AT Cuts at: 3602 3602 Size: 9302 DraI TTT'AAA Cuts at: 6301 6301 Size: 9302 DraIII CAC_nnn'GTG Cuts at: 2218 2218 Size: 9302 DrdI GACnn_nn'nnGTC Cuts at: 421 4051 7008 7866 421 Size: 3630 2957 858 1857 Fragments arranged by size: 3630 2957 1857 858 EaeI y'GGCC_r Cuts at: 241 305 434 449 703 921 963 1223 1250 Size: 64 129 15 254 218 42 260 27 Cuts at: 1250 3317 3638 5471 6003 6018 6887 7061 7452 Size: 2067 321 1833 532 15 869 174 391 Cuts at: 7452 7479 8824 241 Size: 27 1345 719 Fragments arranged by size: 2067 1833 1345 869 719 532 391 321 260 254 218 174 129 64 42 27 27 15 15 EagI C'GGCC_G Cuts at: 241 434 449 703 6887 241 Size: 193 15 254 6184 2656 Fragments arranged by size: 6184 2656 254 193 15 EarI CTCTTCn'nnn_ Cuts at: 508 863 2345 2474 4036 7325 7535 508 Size: 355 1482 129 1562 3289 210 2275 Fragments arranged by size: 3289 2275 1562 1482 355 210 129 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 127 1142 2215 2851 3904 4737 7510 7820 7966 Size: 1015 1073 636 1053 833 2773 310 146 Cuts at: 7966 9112 127 Size: 1146 317 Fragments arranged by size: 2773 1146 1073 1053 1015 833 636 317 310 146 EcoICRI GAG'CTC Cuts at: 1586 1818 2013 3465 5324 6314 1586 Size: 232 195 1452 1859 990 4574 Fragments arranged by size: 4574 1859 1452 990 232 195 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 184 192 504 840 914 1445 1819 2234 2369 Size: 8 312 336 74 531 374 415 135 Cuts at: 2369 2663 2952 3415 3490 3511 3799 3841 3934 Size: 294 289 463 75 21 288 42 93 Cuts at: 3934 4087 4435 4582 4814 5076 5238 5395 5674 Size: 153 348 147 232 262 162 157 279 Cuts at: 5674 5697 6145 7127 7559 8306 8574 184 Size: 23 448 982 432 747 268 912 Fragments arranged by size: 982 912 747 531 463 448 432 415 374 348 336 312 294 289 288 279 268 262 232 162 157 153 147 135 93 75 74 42 23 21 8 EcoNI CCTnn'n_nnAGG Cuts at: 2776 3373 3493 3820 3976 4249 4978 6004 2776 Size: 597 120 327 156 273 729 1026 6074 Fragments arranged by size: 6074 1026 729 597 327 273 156 120 EcoO109I rG'GnC_Cy Cuts at: 299 407 456 785 876 1168 1420 1831 2020 Size: 108 49 329 91 292 252 411 189 Cuts at: 2020 2184 2303 2524 2637 2638 2768 2769 3162 Size: 164 119 221 113 1 130 1 393 Cuts at: 3162 3771 3954 4254 4335 4359 5289 5520 6103 Size: 609 183 300 81 24 930 231 583 Cuts at: 6103 6154 6441 299 Size: 51 287 3160 Fragments arranged by size: 3160 930 609 583 411 393 329 300 292 287 252 231 221 189 183 164 130 119 113 108 91 81 51 49 24 1 1 EcoRI G'AATT_C Cuts at: 6306 6306 Size: 9302 EcoRV GAT'ATC Cuts at: 1506 2494 1506 Size: 988 8314 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1251 7321 1251 Size: 6070 3232 FspI TGC'GCA Cuts at: 496 3364 7083 496 Size: 2868 3719 2715 Fragments arranged by size: 3719 2868 2715 HaeII r_GCGC'y Cuts at: 171 372 536 2331 2883 3712 3897 4381 5887 Size: 201 164 1795 552 829 185 484 1506 Cuts at: 5887 6736 6984 8006 8985 171 Size: 849 248 1022 979 488 Fragments arranged by size: 1795 1506 1022 979 849 829 552 488 484 248 201 185 164 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 382 414 529 561 1306 1338 1495 1527 1615 Size: 32 115 32 745 32 157 32 88 Cuts at: 1615 1647 1710 1742 2225 2257 2436 2468 2830 Size: 32 63 32 483 32 179 32 362 Cuts at: 2830 2862 3909 3941 5005 5017 5037 5049 5612 Size: 32 1047 32 1064 12 20 12 563 Cuts at: 5612 5644 6240 6272 7144 7174 7176 7206 382 Size: 32 596 32 872 30 2 30 2478 Fragments arranged by size: 2478 1064 1047 872 745 596 563 483 362 179 157 115 88 63 32 32 32 32 32 32 32 32 32 32 32 32 30 30 20 12 12 2 HincII GTy'rAC Cuts at: 1477 5304 6341 1477 Size: 3827 1037 4438 Fragments arranged by size: 4438 3827 1037 KasI G'GCGC_C Cuts at: 6732 6980 6732 Size: 248 9054 KpnI G_GTAC'C Cuts at: 2099 6322 2099 Size: 4223 5079 MluI A'CGCG_T Cuts at: 8735 8735 Size: 9302 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 4346 5399 6240 7973 8157 4346 Size: 1053 841 1733 184 5491 Fragments arranged by size: 5491 1733 1053 841 184 MscI TGG'CCA Cuts at: 1225 1252 3319 3640 5473 7063 1225 Size: 27 2067 321 1833 1590 3464 Fragments arranged by size: 3464 2067 1833 1590 321 27 MslI CAynn'nnrTG Cuts at: 490 2104 2987 3004 3155 3370 3902 3994 5575 Size: 1614 883 17 151 215 532 92 1581 Cuts at: 5575 7291 7418 490 Size: 1716 127 2374 Fragments arranged by size: 2374 1716 1614 1581 883 532 215 151 127 92 17 MspA1I CmG'CkG Cuts at: 403 433 439 739 888 971 1271 1482 1854 Size: 30 6 300 149 83 300 211 372 Cuts at: 1854 1998 2715 3393 3741 3831 4170 4345 4631 Size: 144 717 678 348 90 339 175 286 Cuts at: 4631 4879 5652 5690 5787 5969 6048 6413 6727 Size: 248 773 38 97 182 79 365 314 Cuts at: 6727 7087 8100 8345 403 Size: 360 1013 245 1360 Fragments arranged by size: 1360 1013 773 717 678 372 365 360 348 339 314 300 300 286 248 245 211 182 175 149 144 97 90 83 79 38 30 6 NaeI GCC'GGC Cuts at: 241 703 7483 241 Size: 462 6780 2060 Fragments arranged by size: 6780 2060 462 NarI GG'CG_CC Cuts at: 6733 6981 6733 Size: 248 9054 NcoI C'CATG_G Cuts at: 107 1766 2148 2429 107 Size: 1659 382 281 6980 Fragments arranged by size: 6980 1659 382 281 NgoMIV G'CCGG_C Cuts at: 239 701 7481 239 Size: 462 6780 2060 Fragments arranged by size: 6780 2060 462 NotI GC'GGCC_GC Cuts at: 434 434 Size: 9302 NspI r_CATG'y Cuts at: 635 3141 3189 3306 3387 3513 3526 3687 4599 Size: 2506 48 117 81 126 13 161 912 Cuts at: 4599 5419 5664 6355 6855 7762 635 Size: 820 245 691 500 907 2175 Fragments arranged by size: 2506 2175 912 907 820 691 500 245 161 126 117 81 48 13 PasI CC'CwG_GG Cuts at: 2155 2535 4266 5556 2155 Size: 380 1731 1290 5901 Fragments arranged by size: 5901 1731 1290 380 PciI A'CATG_T Cuts at: 5660 7758 5660 Size: 2098 7204 PflMI CCAn_nnn'nTGG Cuts at: 742 1920 4317 5188 5233 742 Size: 1178 2397 871 45 4811 Fragments arranged by size: 4811 2397 1178 871 45 PfoI T'CCnGG_A Cuts at: 520 1130 1868 3467 3488 520 Size: 610 738 1599 21 6334 Fragments arranged by size: 6334 1599 738 610 21 PmeI GTTT'AAAC Cuts at: 6301 6301 Size: 9302 PmlI CAC'GTG Cuts at: 3315 5361 5754 3315 Size: 2046 393 6863 Fragments arranged by size: 6863 2046 393 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3136 3168 8441 8473 3136 Size: 32 5273 32 3965 Fragments arranged by size: 5273 3965 32 32 PpuMI rG'GwC_Cy Cuts at: 407 1168 1420 2303 3162 3771 4254 4335 4359 Size: 761 252 883 859 609 483 81 24 Cuts at: 4359 5289 5520 407 Size: 930 231 4189 Fragments arranged by size: 4189 930 883 859 761 609 483 252 231 81 24 PspOMI G'GGCC_C Cuts at: 298 456 785 876 2183 2637 2768 2954 298 Size: 158 329 91 1307 454 131 186 6646 Fragments arranged by size: 6646 1307 454 329 186 158 131 91 PspXI vC'TCGA_Gb Cuts at: 3115 3115 Size: 9302 PstI C_TGCA'G Cuts at: 1027 3255 3286 3914 4787 4884 5063 5651 6042 Size: 2228 31 628 873 97 179 588 391 Cuts at: 6042 6349 7034 1027 Size: 307 685 3295 Fragments arranged by size: 3295 2228 873 685 628 588 391 307 179 97 31 PvuI CG_AT'CG Cuts at: 104 4007 104 Size: 3903 5399 PvuII CAG'CTG Cuts at: 888 4345 4631 5652 5787 5969 6048 6727 7087 Size: 3457 286 1021 135 182 79 679 360 Cuts at: 7087 888 Size: 3103 Fragments arranged by size: 3457 3103 1021 679 360 286 182 135 79 RsrII CG'GwC_CG Cuts at: 7497 7497 Size: 9302 SacI G_AGCT'C Cuts at: 1588 1820 2015 3467 5326 6316 1588 Size: 232 195 1452 1859 990 4574 Fragments arranged by size: 4574 1859 1452 990 232 195 SacII CC_GC'GG Cuts at: 434 440 1272 434 Size: 6 832 8464 Fragments arranged by size: 8464 832 6 SalI G'TCGA_C Cuts at: 1475 5302 6339 1475 Size: 3827 1037 4438 Fragments arranged by size: 4438 3827 1037 SanDI GG'GwC_CC Cuts at: 407 5520 407 Size: 5113 4189 SapI GCTCTTCn'nnn_ Cuts at: 508 2345 7325 7535 508 Size: 1837 4980 210 2275 Fragments arranged by size: 4980 2275 1837 210 SbfI CC_TGCA'GG Cuts at: 6349 6349 Size: 9302 ScaI AGT'ACT Cuts at: 8819 8819 Size: 9302 SexAI A'CCwGG_T Cuts at: 1575 2214 2733 5453 1575 Size: 639 519 2720 5424 Fragments arranged by size: 5424 2720 639 519 SfcI C'TryA_G Cuts at: 33 1023 2298 3251 3282 3910 4783 4880 5059 Size: 990 1275 953 31 628 873 97 179 Cuts at: 5059 5647 6038 6345 7030 8023 8214 33 Size: 588 391 307 685 993 191 1121 Fragments arranged by size: 1275 1121 993 990 953 873 685 628 588 391 307 191 179 97 31 SfoI GGC'GCC Cuts at: 6734 6982 6734 Size: 248 9054 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 9302 SmaI CCC'GGG Cuts at: 186 259 790 987 4779 5275 6324 186 Size: 73 531 197 3792 496 1049 3164 Fragments arranged by size: 3792 3164 1049 531 496 197 73 SmlI C'TyrA_G Cuts at: 219 1303 1708 3115 3664 5284 6457 7372 7864 Size: 1084 405 1407 549 1620 1173 915 492 Cuts at: 7864 8126 219 Size: 262 1395 Fragments arranged by size: 1620 1407 1395 1173 1084 915 549 492 405 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 9302 SphI G_CATG'C Cuts at: 3513 3526 3687 4599 5419 6355 6855 3513 Size: 13 161 912 820 936 500 5960 Fragments arranged by size: 5960 936 912 820 500 161 13 SrfI GCCC'GGGC Cuts at: 790 987 4779 790 Size: 197 3792 5313 Fragments arranged by size: 5313 3792 197 StuI AGG'CCT Cuts at: 996 1029 2296 3654 4501 4729 5314 5999 6171 Size: 33 1267 1358 847 228 585 685 172 Cuts at: 6171 996 Size: 4127 Fragments arranged by size: 4127 1358 1267 847 685 585 228 172 33 StyI C'CwwG_G Cuts at: 107 1226 1766 2148 2429 3566 3641 4296 4485 Size: 1119 540 382 281 1137 75 655 189 Cuts at: 4485 5123 5546 6142 6436 6790 107 Size: 638 423 596 294 354 2619 Fragments arranged by size: 2619 1137 1119 655 638 596 540 423 382 354 294 281 189 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 700 962 1912 5569 5578 5710 5718 5901 6885 Size: 262 950 3657 9 132 8 183 984 Cuts at: 6885 7018 7477 7686 700 Size: 133 459 209 2316 Fragments arranged by size: 3657 2316 984 950 459 262 209 183 133 132 9 8 TatI w'GTAC_w Cuts at: 3134 4109 4145 8817 3134 Size: 975 36 4672 3619 Fragments arranged by size: 4672 3619 975 36 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5471 5503 5893 5925 6121 6153 8819 8851 5471 Size: 32 390 32 196 32 2666 32 5922 Fragments arranged by size: 5922 2666 390 196 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 7099 7099 Size: 9302 XbaI T'CTAG_A Cuts at: 4545 6333 4545 Size: 1788 7514 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1936 2152 2427 3327 4521 5574 6052 6797 1936 Size: 216 275 900 1194 1053 478 745 4441 Fragments arranged by size: 4441 1194 1053 900 745 478 275 216 XhoI C'TCGA_G Cuts at: 3115 3115 Size: 9302 XmaI C'CCGG_G Cuts at: 184 257 788 985 4777 5273 6322 184 Size: 73 531 197 3792 496 1049 3164 Fragments arranged by size: 3792 3164 1049 531 496 197 73 ZraI GAC'GTC Cuts at: 3123 4869 5802 3123 Size: 1746 933 6623 Fragments arranged by size: 6623 1746 933 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI Enzymes that do not cut: AflII AscI AvrII BaeI BfrBI BmtI BsiWI BsmI BstZ17I CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI NruI NsiI PacI PshAI PsiI PsrI SfiI SgrAI SnaBI SspI SwaI XmnI