Restriction map for pF1KE2527
 (Linear) (Six-base) MAPSORT of: KIEE2527.seq  Check: 9834  from: 1  to: 5610



KIEE2527.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4813    5610 
   Size:       4813     797
 
AatII G_ACGT'C

Cuts at:      0    4101    5610 
   Size:       4101    1509
 
AccI GT'mk_AC

Cuts at:      0     735    4995    5484    5610 
   Size:        735    4260     489     126

  Fragments arranged by size: 

                4260     735     489     126
 
AclI AA'CG_TT

Cuts at:      0      70    2707    5610 
   Size:         70    2637    2903

  Fragments arranged by size: 

                2903    2637      70
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665    1546    1901    3223    3238    3301    3494    3568 
   Size:        665     881     355    1322      15      63     193      74

Cuts at:   3568    3800    4276    4310    4623    5300    5610 
   Size:        232     476      34     313     677     310

  Fragments arranged by size: 

                1322     881     677     665     476     355     313     310
                 232     193      74      63      34      15
 
AflII C'TTAA_G

Cuts at:      0    1707    2209    2890    5610 
   Size:       1707     502     681    2720

  Fragments arranged by size: 

                2720    1707     681     502
 
AleI CACnn'nnGTG

Cuts at:      0    4862    5610 
   Size:       4862     748
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    4718    4750    5610 
   Size:       4718      32     860

  Fragments arranged by size: 

                4718     860      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    3706    5518    5610 
   Size:       3706    1812      92

  Fragments arranged by size: 

                3706    1812      92
 
ApoI r'AATT_y

Cuts at:      0      11     259     418     431     888    1018    1575    1641 
   Size:         11     248     159      13     457     130     557      66

Cuts at:   1641    1769    1819    1992    2085    2580    3327    3804    3859 
   Size:        128      50     173      93     495     747     477      55

Cuts at:   3859    3951    3986    4141    4527    4870    5574    5610 
   Size:         92      35     155     386     343     704      36

  Fragments arranged by size: 

                 747     704     557     495     477     457     386     343
                 248     173     159     155     130     128      93      92
                  66      55      50      36      35      13      11
 
AseI AT'TA_AT

Cuts at:      0     164    1222    3139    3997    4226    5610 
   Size:        164    1058    1917     858     229    1384

  Fragments arranged by size: 

                1917    1384    1058     858     229     164
 
BanII G_rGCy'C

Cuts at:      0    2228    3055    5610 
   Size:       2228     827    2555

  Fragments arranged by size: 

                2555    2228     827
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1058    1097    1552    1694    2755    3080    3386    3443 
   Size:       1058      39     455     142    1061     325     306      57

Cuts at:   3443    3599    3808    4691    5610 
   Size:        156     209     883     919

  Fragments arranged by size: 

                1061    1058     919     883     455     325     306     209
                 156     142      57      39
 
BclI T'GATC_A

Cuts at:      0     290    3464    5610 
   Size:        290    3174    2146

  Fragments arranged by size: 

                3174    2146     290
 
BlpI GC'TnA_GC

Cuts at:      0    1607    1807    2162    3934    5610 
   Size:       1607     200     355    1772    1676

  Fragments arranged by size: 

                1772    1676    1607     355     200
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    4951    5610 
   Size:       4951     659
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3074    5610 
   Size:       3074    2536
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     695    4260    4948    5008    5610 
   Size:         49     646    3565     688      60     602

  Fragments arranged by size: 

                3565     688     646     602      60      49
 
Bpu10I CC'TnA_GC

Cuts at:      0     787    5610 
   Size:        787    4823
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     862    2815    3232    3589    5082    5312    5610 
   Size:        862    1953     417     357    1493     230     298

  Fragments arranged by size: 

                1953    1493     862     417     357     298     230
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     664    3578    5253    5610 
   Size:        664    2914    1675     357

  Fragments arranged by size: 

                2914    1675     664     357
 
BsaAI yAC'GTr

Cuts at:      0    2722    5610 
   Size:       2722    2888
 
BsaBI GATnn'nnATC

Cuts at:      0    4226    5610 
   Size:       4226    1384
 
BsaHI Gr'CG_yC

Cuts at:      0    4098    5610 
   Size:       4098    1512
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    3047    4148    5610 
   Size:       3047    1101    1462

  Fragments arranged by size: 

                3047    1462    1101
 
BseYI C'CCAG_C

Cuts at:      0    1289    5610 
   Size:       1289    4321
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     674    1751    4504    4847    5610 
   Size:        413     261    1077    2753     343     763

  Fragments arranged by size: 

                2753    1077     763     413     343     261
 
BsiHKAI G_wGCw'C

Cuts at:      0    1116    2228    3055    4964    5610 
   Size:       1116    1112     827    1909     646

  Fragments arranged by size: 

                1909    1116    1112     827     646
 
BsmI GAATG_Cn'

Cuts at:      0     230    3312    3375    5610 
   Size:        230    3082      63    2235

  Fragments arranged by size: 

                3082    2235     230      63
 
Bsp1286I G_dGCh'C

Cuts at:      0    1116    2228    3055    4964    5610 
   Size:       1116    1112     827    1909     646

  Fragments arranged by size: 

                1909    1116    1112     827     646
 
BspHI T'CATG_A

Cuts at:      0      38     293    1173    1776    2787    3359    3474    3495 
   Size:         38     255     880     603    1011     572     115      21

Cuts at:   3495    3693    4884    5610 
   Size:        198    1191     726

  Fragments arranged by size: 

                1191    1011     880     726     603     572     255     198
                 115      38      21
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     524    4813    5576    5610 
   Size:        524    4289     763      34

  Fragments arranged by size: 

                4289     763     524      34
 
BsrBI CCG'CTC

Cuts at:      0     651    4268    5610 
   Size:        651    3617    1342

  Fragments arranged by size: 

                3617    1342     651
 
BsrDI GCAATG_nn'

Cuts at:      0     526     580    1470    2383    5610 
   Size:        526      54     890     913    3227

  Fragments arranged by size: 

                3227     913     890     526      54
 
BssSI C'ACGA_G

Cuts at:      0    1109    5405    5610 
   Size:       1109    4296     205

  Fragments arranged by size: 

                4296    1109     205
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     466    5610 
   Size:        466    5144
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      51    4653    4864    5610 
   Size:         51    4602     211     746

  Fragments arranged by size: 

                4602     746     211      51
 
BstYI r'GATC_y

Cuts at:      0    1587    4044    4642    5610 
   Size:       1587    2457     598     968

  Fragments arranged by size: 

                2457    1587     968     598
 
BtgI C'CryG_G

Cuts at:      0    4490    5610 
   Size:       4490    1120
 
BtsI GCAGTG_nn'

Cuts at:      0    3166    4869    4914    5534    5610 
   Size:       3166    1703      45     620      76

  Fragments arranged by size: 

                3166    1703     620      76      45
 
DraI TTT'AAA

Cuts at:      0    1952    2046    3770    5610 
   Size:       1952      94    1724    1840

  Fragments arranged by size: 

                1952    1840    1724      94
 
DraIII CAC_nnn'GTG

Cuts at:      0     703    2722    4702    5610 
   Size:        703    2019    1980     908

  Fragments arranged by size: 

                2019    1980     908     703
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     540    5610 
   Size:        540    5070
 
EaeI y'GGCC_r

Cuts at:      0      83    4353    5189    5610 
   Size:         83    4270     836     421

  Fragments arranged by size: 

                4270     836     421      83
 
EarI CTCTTCn'nnn_

Cuts at:      0     641     687    1424    3299    4112    4632    5151    5610 
   Size:        641      46     737    1875     813     520     519     459

  Fragments arranged by size: 

                1875     813     737     641     520     519     459      46
            
 
EcoICRI GAG'CTC

Cuts at:      0    2226    3053    5610 
   Size:       2226     827    2557

  Fragments arranged by size: 

                2557    2226     827
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     665     695    1546    1901    3223    3238    3301 
   Size:         49     616      30     851     355    1322      15      63

Cuts at:   3301    3494    3568    3800    4260    4276    4310    4623    4948 
   Size:        193      74     232     460      16      34     313     325

Cuts at:   4948    5008    5300    5610 
   Size:         60     292     310

  Fragments arranged by size: 

                1322     851     616     460     355     325     313     310
                 292     232     193      74      63      60      49      34
                  30      16      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    4410    5318    5610 
   Size:       4410     908     292

  Fragments arranged by size: 

                4410     908     292
 
EcoRI G'AATT_C

Cuts at:      0     431    2085    5610 
   Size:        431    1654    3525

  Fragments arranged by size: 

                3525    1654     431
 
EcoRV GAT'ATC

Cuts at:      0    1307    5610 
   Size:       1307    4303
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1186    1627    2224    4306    5128    5610 
   Size:       1186     441     597    2082     822     482

  Fragments arranged by size: 

                2082    1186     822     597     482     441
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     892     924    1357    1389    1421    2361    2393    3430 
   Size:        892      32     433      32      32     940      32    1037

Cuts at:   3430    3456    3462    3488    3505    3537    3886    3918    4660 
   Size:         26       6      26      17      32     349      32     742

Cuts at:   4660    4692    4885    4917    4983    5015    5250    5282    5610 
   Size:         32     193      32      66      32     235      32     328

  Fragments arranged by size: 

                1037     940     892     742     433     349     328     235
                 193      66      32      32      32      32      32      32
                  32      32      32      32      26      26      17       6
            
 
HincII GTy'rAC

Cuts at:      0     736    1370    3763    4623    4996    5610 
   Size:        736     634    2393     860     373     614

  Fragments arranged by size: 

                2393     860     736     634     614     373
 
HindIII A'AGCT_T

Cuts at:      0     970    3313    5071    5610 
   Size:        970    2343    1758     539

  Fragments arranged by size: 

                2343    1758     970     539
 
MfeI C'AATT_G

Cuts at:      0     286    2497    2971    3889    5610 
   Size:        286    2211     474     918    1721

  Fragments arranged by size: 

                2211    1721     918     474     286
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     803    1067    2547    3611    5610 
   Size:        803     264    1480    1064    1999

  Fragments arranged by size: 

                1999    1480    1064     803     264
 
MscI TGG'CCA

Cuts at:      0    4355    5191    5610 
   Size:       4355     836     419

  Fragments arranged by size: 

                4355     836     419
 
MslI CAynn'nnrTG

Cuts at:      0     244    2155    4468    4862    4889    5610 
   Size:        244    1911    2313     394      27     721

  Fragments arranged by size: 

                2313    1911     721     394     244      27
 
MspA1I CmG'CkG

Cuts at:      0     463    1716    2436    5515    5610 
   Size:        463    1253     720    3079      95

  Fragments arranged by size: 

                3079    1253     720     463      95
 
NcoI C'CATG_G

Cuts at:      0    4490    5610 
   Size:       4490    1120
 
PasI CC'CwG_GG

Cuts at:      0    1347    5215    5610 
   Size:       1347    3868     395

  Fragments arranged by size: 

                3868    1347     395
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3067    3891    4362    5610 
   Size:       3067     824     471    1248

  Fragments arranged by size: 

                3067    1248     824     471
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1098    1130    5610 
   Size:       1098      32    4480

  Fragments arranged by size: 

                4480    1098      32
 
PshAI GACnn'nnGTC

Cuts at:      0    4691    5610 
   Size:       4691     919
 
PsiI TTA'TAA

Cuts at:      0    1025    1859    2963    5610 
   Size:       1025     834    1104    2647

  Fragments arranged by size: 

                2647    1104    1025     834
 
PstI C_TGCA'G

Cuts at:      0    3711    4635    5541    5610 
   Size:       3711     924     906      69

  Fragments arranged by size: 

                3711     924     906      69
 
PvuII CAG'CTG

Cuts at:      0    2436    5515    5610 
   Size:       2436    3079      95

  Fragments arranged by size: 

                3079    2436      95
 
SacI G_AGCT'C

Cuts at:      0    2228    3055    5610 
   Size:       2228     827    2555

  Fragments arranged by size: 

                2555    2228     827
 
SalI G'TCGA_C

Cuts at:      0     734    4994    5610 
   Size:        734    4260     616

  Fragments arranged by size: 

                4260     734     616
 
SapI GCTCTTCn'nnn_

Cuts at:      0     641    1424    5610 
   Size:        641     783    4186

  Fragments arranged by size: 

                4186     783     641
 
ScaI AGT'ACT

Cuts at:      0    2844    4521    4930    5610 
   Size:       2844    1677     409     680

  Fragments arranged by size: 

                2844    1677     680     409
 
SexAI A'CCwGG_T

Cuts at:      0     146    4478    5262    5610 
   Size:        146    4332     784     348

  Fragments arranged by size: 

                4332     784     348     146
 
SfcI C'TryA_G

Cuts at:      0    1454    2167    3707    3756    4003    4598    4631    5537 
   Size:       1454     713    1540      49     247     595      33     906

Cuts at:   5537    5610 
   Size:         73

  Fragments arranged by size: 

                1540    1454     906     713     595     247      73      49
                  33
 
SmlI C'TyrA_G

Cuts at:      0     841    1707    2209    2794    2890    3211    3568    5097 
   Size:        841     866     502     585      96     321     357    1529

Cuts at:   5097    5327    5610 
   Size:        230     283

  Fragments arranged by size: 

                1529     866     841     585     502     357     321     283
                 230      96
 
SpeI A'CTAG_T

Cuts at:      0    1315    5610 
   Size:       1315    4295
 
StuI AGG'CCT

Cuts at:      0    1978    4447    5610 
   Size:       1978    2469    1163

  Fragments arranged by size: 

                2469    1978    1163
 
StyI C'CwwG_G

Cuts at:      0    4361    4490    5474    5610 
   Size:       4361     129     984     136

  Fragments arranged by size: 

                4361     984     136     129
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    4275    4437    5610 
   Size:       4275     162    1173

  Fragments arranged by size: 

                4275    1173     162
 
TatI w'GTAC_w

Cuts at:      0    2842    4070    4519    4928    5082    5559    5610 
   Size:       2842    1228     449     409     154     477      51

  Fragments arranged by size: 

                2842    1228     477     449     409     154      51
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     220     252    5610 
   Size:        220      32    5358

  Fragments arranged by size: 

                5358     220      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3267    5264    5610 
   Size:       3267    1997     346

  Fragments arranged by size: 

                3267    1997     346
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4476    5610 
   Size:       4476    1134
 
XmnI GAAnn'nnTTC

Cuts at:      0     431    1433    3491    4286    5610 
   Size:        431    1002    2058     795    1324

  Fragments arranged by size: 

                2058    1324    1002     795     431
 
ZraI GAC'GTC

Cuts at:      0    4099    5610 
   Size:       4099    1511
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI    AflII     AleI     AloI
    AlwNI     ApoI     AseI    BanII     BbsI     BclI     BlpI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BssSI   BstAPI    BstXI    BstYI     BtgI     BtsI
     DraI   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII     MfeI     MmeI
     MscI     MslI   MspA1I     NcoI     PasI    PflMI     PpiI    PshAI
     PsiI     PstI    PvuII     SacI     SalI     SapI     ScaI    SexAI
     SfcI     SmlI     SpeI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AfeI   AflIII     AgeI     AhdI     AlfI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmtI    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BsrFI
    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     EagI     EciI EcoO109I     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PfoI
     PmeI     PmlI    PpuMI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SphI     SrfI     SspI     SwaI     XbaI     XhoI     XmaI
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