################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI AocI AscI Asp718I AspEI AvrII BamHI BbvCI BcgI BglII BlnI BsiEI BsiWI BspDI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI FseI HpaI KpnI MluI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI SalI SbfI SfiI SfuI SgrAI SnaBI SphI Sse8387I StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 2462 GTAGAC GT^[AC][GT]AC AccIII 2679 TCCGGA T^CCGGA AflII 1617 CTTAAG C^TTAAG Alw44I 3168 GTGCAC G^TGCAC Aor51HI 2854 AGCGCT AGC^GCT ApaI 795 GGGCCC GGGCC^C ApaLI 3168 GTGCAC G^TGCAC AspI 2650 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2190 TGCGCA TGC^GCA BbeI 20 GGCGCC GGCGC^C BfrI 1617 CTTAAG C^TTAAG BglI 2886 GCCCGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseAI 2679 TCCGGA T^CCGGA BsgI 1849 GTGCAG GTGCAG(16/14) Bsp1407I 440 TGTACA T^GTACA BspEI 2679 TCCGGA T^CCGGA BspHI 2294 TCATGA T^CATGA BspMI 32 ACCTGC ACCTGC(4/8) BsrFI 744 GCCGGC [GA]^CCGG[CT] BsrGI 440 TGTACA T^GTACA BssSI 1858 CTCGTG CACGAG(-5/-1) BstAPI 2895 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3123 GGTTACC G^GT[ACGT]ACC BstPI 3123 GGTTACC G^GT[ACGT]ACC Cfr10I 744 GCCGGC [GA]^CCGG[CT] CpoI 1472 CGGTCCG CG^G[AT]CCG DraI 1356 TTTAAA TTT^AAA DraIII 1366 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2854 AGCGCT AGC^GCT EcoO65I 3123 GGTTACC G^GT[ACGT]ACC EcoT22I 990 ATGCAT ATGCA^T FspI 2190 TGCGCA TGC^GCA HincII 713 GTCAAC GT[CT]^[GA]AC HindII 713 GTCAAC GT[CT]^[GA]AC HindIII 2151 AAGCTT A^AGCTT KasI 20 GGCGCC G^GCGCC KspI 79 CCGCGG CCGC^GG MfeI 1679 CAATTG C^AATTG MroI 2679 TCCGGA T^CCGGA MunI 1679 CAATTG C^AATTG NaeI 744 GCCGGC GCC^GGC NarI 20 GGCGCC GG^CGCC NgoMIV 744 GCCGGC G^CCGGC NsiI 990 ATGCAT ATGCA^T PflFI 2650 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2346 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 2226 CAGCTG CAG^CTG RcaI 2294 TCATGA T^CATGA RsrII 1472 CGGTCCG CG^G[AT]CCG SacII 79 CCGCGG CCGC^GG SapI 643 GCTCTTC GCTCTTC(1/4) ScaI 1758 AGTACT AGT^ACT SfoI 20 GGCGCC GGC^GCC SpeI 930 ACTAGT A^CTAGT SspBI 440 TGTACA T^GTACA SspI 2474 AATATT AAT^ATT Tth111I 2650 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2346 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 20 GGCGCC G[GA]^CG[CT]C 469 GACGCC AseI 1690 ATTAAT AT^TAAT 2070 ATTAAT AsnI 1690 ATTAAT AT^TAAT 2070 ATTAAT BbrPI 2587 CACGTG CAC^GTG 3165 CACGTG BciVI 361 GGATAC GTATCC(6/5) 3160 GGATAC BfaI 931 CTAG C^TAG 1762 CTAG BlpI 516 GCTTAGC GC^T[ACGT]AGC 2593 GCTGAGC Bpu1102I 516 GCTTAGC GC^T[ACGT]AGC 2593 GCTGAGC BsaAI 2587 CACGTG [CT]AC^GT[GA] 3165 CACGTG BsaBI 273 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2286 GATGTTGATC BsaHI 20 GGCGCC G[GA]^CG[CT]C 469 GACGCC BsaI 2382 GAGACC GGTCTC(1/5) 2536 GAGACC BsaWI 2679 TCCGGA [AT]^CCGG[AT] 3100 ACCGGA BspLU11I 1091 ACATGT A^CATGT 2090 ACATGT BstYI 569 AGATCC [GA]^GATC[CT] 2036 GGATCT CelII 516 GCTTAGC GC^T[ACGT]AGC 2593 GCTGAGC Hin1I 20 GGCGCC G[GA]^CG[CT]C 469 GACGCC MaeI 931 CTAG C^TAG 1762 CTAG MamI 273 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2286 GATGTTGATC MflI 569 AGATCC [GA]^GATC[CT] 2036 GGATCT MspA1I 79 CCGCGG C[AC]G^C[GT]G 2226 CAGCTG PmaCI 2587 CACGTG CAC^GTG 3165 CACGTG PmlI 2587 CACGTG CAC^GTG 3165 CACGTG PshBI 1690 ATTAAT AT^TAAT 2070 ATTAAT SacI 949 GAGCTC GAGCT^C 3035 GAGCTC SmaI 86 CCCGGG CCC^GGG 921 CCCGGG SmlI 1617 CTTAAG C^T[CT][GA]AG 1711 CTCAAG TaqI 293 TCGA T^CGA 3177 TCGA TthHB8I 293 TCGA T^CGA 3177 TCGA XcmI 1242 CCACATGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1440 CCACACCCTTGATGG XhoII 569 AGATCC [GA]^GATC[CT] 2036 GGATCT XmaCI 86 CCCGGG C^CCGGG 921 CCCGGG XmaI 86 CCCGGG C^CCGGG 921 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI