(Circular) (Six-base) MAPSORT of: pF1KE2569.seq Check: 7648 from: 1 to: 6386 pF1KE2569 6386 bp With 182 enzymes: SgfI * April 14, 2015 09:47 .. AccI GT'mk_AC Cuts at: 2571 3424 2571 Size: 853 5533 Acc65I G'GTAC_C Cuts at: 3402 3402 Size: 6386 AclI AA'CG_TT Cuts at: 6226 6226 Size: 6386 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 739 1521 1847 2410 2533 2746 3340 4211 4643 Size: 782 326 563 123 213 594 871 432 Cuts at: 4643 5390 5658 739 Size: 747 268 1467 Fragments arranged by size: 1467 871 782 747 594 563 432 326 268 213 123 AfeI AGC'GCT Cuts at: 2964 2964 Size: 6386 AflII C'TTAA_G Cuts at: 1725 1725 Size: 6386 AflIII A'CryG_T Cuts at: 1199 1377 2198 3272 3738 4842 5819 1199 Size: 178 821 1074 466 1104 977 1766 Fragments arranged by size: 1766 1104 1074 977 821 466 178 AgeI A'CCGG_T Cuts at: 4774 4774 Size: 6386 AleI CACnn'nnGTG Cuts at: 619 3025 619 Size: 2406 3980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1038 1038 Size: 6386 AlwNI CAG_nnn'CTG Cuts at: 681 897 1476 2061 3691 5258 681 Size: 216 579 585 1630 1567 1809 Fragments arranged by size: 1809 1630 1567 585 579 216 ApaI G_GGCC'C Cuts at: 907 907 Size: 6386 ApaLI G'TGCA_C Cuts at: 3276 5156 3276 Size: 1880 4506 ApoI r'AATT_y Cuts at: 808 1112 2304 2617 2800 2899 3390 5739 5995 Size: 304 1192 313 183 99 491 2349 256 Cuts at: 5995 808 Size: 1199 Fragments arranged by size: 2349 1199 1192 491 313 304 256 183 99 AseI AT'TA_AT Cuts at: 20 1799 2179 20 Size: 1779 380 4227 Fragments arranged by size: 4227 1779 380 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6386 AvaI C'yCGr_G Cuts at: 194 310 1029 2112 2995 3406 194 Size: 116 719 1083 883 411 3174 Fragments arranged by size: 3174 1083 883 719 411 116 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1673 1706 1673 Size: 33 6353 BamHI G'GATC_C Cuts at: 3411 3411 Size: 6386 BanI G'GyrC_C Cuts at: 128 493 3019 3402 3816 4064 4099 128 Size: 365 2526 383 414 248 35 2415 Fragments arranged by size: 2526 2415 414 383 365 248 35 BanII G_rGCy'C Cuts at: 277 907 1061 1525 2527 2996 3147 3400 4430 Size: 630 154 464 1002 469 151 253 1030 Cuts at: 4430 277 Size: 2233 Fragments arranged by size: 2233 1030 1002 630 469 464 253 154 151 BbeI G_GCGC'C Cuts at: 132 3820 4068 132 Size: 3688 248 2450 Fragments arranged by size: 3688 2450 248 BbsI GAAGACnn'nnnn_ Cuts at: 243 291 332 1835 2521 3328 243 Size: 48 41 1503 686 807 3301 Fragments arranged by size: 3301 1503 807 686 48 41 BciVI GTATCCnnnnn_n' Cuts at: 463 3262 4284 5045 6386 463 Size: 2799 1022 761 1341 463 Fragments arranged by size: 2799 1341 1022 761 463 BclI T'GATC_A Cuts at: 692 1074 1496 2399 3906 692 Size: 382 422 903 1507 3172 Fragments arranged by size: 3172 1507 903 422 382 BfrBI ATG'CAT Cuts at: 1100 1100 Size: 6386 BglI GCCn_nnn'nGGC Cuts at: 3000 3000 Size: 6386 BglII A'GATC_T Cuts at: 3901 5938 3901 Size: 2037 4349 BlpI GC'TnA_GC Cuts at: 625 2702 3498 625 Size: 2077 796 3513 Fragments arranged by size: 3513 2077 796 Bme1580I G_kGCm'C Cuts at: 907 2972 3280 4011 4104 5160 907 Size: 2065 308 731 93 1056 2133 Fragments arranged by size: 2133 2065 1056 731 308 93 BmgBI CAC'GTC Cuts at: 1378 2457 2820 1378 Size: 1079 363 4944 Fragments arranged by size: 4944 1079 363 BmrI ACTGGGnnnn_n' Cuts at: 3598 3679 4014 6158 3598 Size: 81 335 2144 3826 Fragments arranged by size: 3826 2144 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4753 4753 Size: 6386 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1256 1382 1423 2695 1256 Size: 126 41 1272 4947 Fragments arranged by size: 4947 1272 126 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1804 3562 4441 4933 5231 1804 Size: 1758 879 492 298 2959 Fragments arranged by size: 2959 1758 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 2484 2638 6090 2484 Size: 154 3452 2780 Fragments arranged by size: 3452 2780 154 BsaAI yAC'GTr Cuts at: 2697 3275 3739 2697 Size: 578 464 5344 Fragments arranged by size: 5344 578 464 BsaBI GATnn'nnATC Cuts at: 6 385 2398 3900 5937 6 Size: 379 2013 1502 2037 455 Fragments arranged by size: 2037 2013 1502 455 379 BsaHI Gr'CG_yC Cuts at: 129 578 3817 4065 4767 6263 129 Size: 449 3239 248 702 1496 252 Fragments arranged by size: 3239 1496 702 449 252 248 BsaWI w'CCGG_w Cuts at: 2787 3208 3575 4096 4774 5048 5195 5606 2787 Size: 421 367 521 678 274 147 411 3567 Fragments arranged by size: 3567 678 521 421 411 367 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 482 486 512 516 1025 1055 2217 2247 3061 Size: 4 26 4 509 30 1162 30 814 Cuts at: 3061 3091 482 Size: 30 3777 Fragments arranged by size: 3777 1162 814 509 30 30 30 26 4 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 172 594 597 1447 2650 2749 3253 172 Size: 422 3 850 1203 99 504 3305 Fragments arranged by size: 3305 1203 850 504 422 99 3 BseYI C'CCAG_C Cuts at: 1526 2971 3811 4569 5146 5642 1526 Size: 1445 840 758 577 496 2270 Fragments arranged by size: 2270 1445 840 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1978 1978 Size: 6386 BsiEI CG_ry'CG Cuts at: 104 3928 3974 5182 104 Size: 3824 46 1208 1308 Fragments arranged by size: 3824 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 665 1061 1409 2708 2984 3147 3280 3400 4178 Size: 396 348 1299 276 163 133 120 778 Cuts at: 4178 4368 5160 665 Size: 190 792 1891 Fragments arranged by size: 1891 1299 792 778 396 348 276 190 163 133 120 BsmI GAATG_Cn' Cuts at: 1102 1266 2339 2845 1102 Size: 164 1073 506 4643 Fragments arranged by size: 4643 1073 506 164 BsmBI CGTCTCn'nnnn_ Cuts at: 170 504 569 3235 170 Size: 334 65 2666 3321 Fragments arranged by size: 3321 2666 334 65 Bsp1286I G_dGCh'C Cuts at: 277 665 907 1061 1409 1525 2527 2708 2972 Size: 388 242 154 348 116 1002 181 264 Cuts at: 2972 2984 2996 3147 3280 3400 4011 4104 4178 Size: 12 12 151 133 120 611 93 74 Cuts at: 4178 4368 4430 5160 277 Size: 190 62 730 1503 Fragments arranged by size: 1503 1002 730 611 388 348 264 242 190 181 154 151 133 120 116 93 74 62 12 12 BspEI T'CCGG_A Cuts at: 2787 5606 2787 Size: 2819 3567 BspHI T'CATG_A Cuts at: 2402 5562 2402 Size: 3160 3226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 149 3436 3650 3952 4333 149 Size: 3287 214 302 381 2202 Fragments arranged by size: 3287 2202 381 302 214 BsrBI CCG'CTC Cuts at: 50 152 243 831 2631 3000 4678 4732 6216 Size: 102 91 588 1800 369 1678 54 1484 Cuts at: 6216 6381 50 Size: 165 55 Fragments arranged by size: 1800 1678 1484 588 369 165 102 91 55 54 BsrDI GCAATG_nn' Cuts at: 584 1819 2071 2120 4298 584 Size: 1235 252 49 2178 2672 Fragments arranged by size: 2672 2178 1235 252 49 BsrFI r'CCGG_y Cuts at: 852 4384 4565 4774 852 Size: 3532 181 209 2464 Fragments arranged by size: 3532 2464 209 181 BsrGI T'GTAC_A Cuts at: 548 548 Size: 6386 BssSI C'ACGA_G Cuts at: 1966 4657 5015 1966 Size: 2691 358 3337 Fragments arranged by size: 3337 2691 358 BstAPI GCAn_nnn'nTGC Cuts at: 3009 3763 3009 Size: 754 5632 BstBI TT'CG_AA Cuts at: 4747 4747 Size: 6386 BstEII G'GTnAC_C Cuts at: 3231 3231 Size: 6386 BstXI CCAn_nnnn'nTGG Cuts at: 1353 2501 3198 1353 Size: 1148 697 4541 Fragments arranged by size: 4541 1148 697 BstYI r'GATC_y Cuts at: 1 677 2144 3411 3878 3901 4236 4482 5494 Size: 676 1467 1267 467 23 335 246 1012 Cuts at: 5494 5580 5592 5938 1 Size: 86 12 346 449 Fragments arranged by size: 1467 1267 1012 676 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 187 464 866 1682 1694 1889 2140 2519 2648 Size: 277 402 816 12 195 251 379 129 Cuts at: 2648 3023 3114 187 Size: 375 91 3459 Fragments arranged by size: 3459 816 402 379 375 277 251 195 129 91 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 136 846 2850 4336 4517 4680 136 Size: 710 2004 1486 181 163 1842 Fragments arranged by size: 2004 1842 1486 710 181 163 BtsI GCAGTG_nn' Cuts at: 1846 1894 2554 3629 1846 Size: 48 660 1075 4603 Fragments arranged by size: 4603 1075 660 48 DraI TTT'AAA Cuts at: 1466 3385 1466 Size: 1919 4467 DraIII CAC_nnn'GTG Cuts at: 1479 1479 Size: 6386 DrdI GACnn_nn'nnGTC Cuts at: 4092 4950 4092 Size: 858 5528 EaeI y'GGCC_r Cuts at: 683 1343 2137 2186 2714 3188 3971 4145 4536 Size: 660 794 49 528 474 783 174 391 Cuts at: 4536 4563 5908 683 Size: 27 1345 1161 Fragments arranged by size: 1345 1161 794 783 660 528 474 391 174 49 27 EagI C'GGCC_G Cuts at: 3971 3971 Size: 6386 EarI CTCTTCn'nnn_ Cuts at: 436 535 758 1540 1741 2041 4409 4619 436 Size: 99 223 782 201 300 2368 210 2203 Fragments arranged by size: 2368 2203 782 300 223 210 201 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 188 4594 4904 5050 6196 188 Size: 4406 310 146 1146 378 Fragments arranged by size: 4406 1146 378 310 146 EcoICRI GAG'CTC Cuts at: 1059 3145 3398 1059 Size: 2086 253 4047 Fragments arranged by size: 4047 2086 253 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 739 1256 1382 1423 1521 1847 2410 2533 2695 Size: 517 126 41 98 326 563 123 162 Cuts at: 2695 2746 3340 4211 4643 5390 5658 739 Size: 51 594 871 432 747 268 1467 Fragments arranged by size: 1467 871 747 594 563 517 432 326 268 162 126 123 98 51 41 EcoO109I rG'GnC_Cy Cuts at: 903 904 1368 1455 1574 3315 3353 3525 903 Size: 1 464 87 119 1741 38 172 3764 Fragments arranged by size: 3764 1741 464 172 119 87 38 1 EcoRI G'AATT_C Cuts at: 3390 3390 Size: 6386 EcoRV GAT'ATC Cuts at: 1245 2354 3165 1245 Size: 1109 811 4466 Fragments arranged by size: 4466 1109 811 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2850 3096 3345 4405 2850 Size: 246 249 1060 4831 Fragments arranged by size: 4831 1060 249 246 FspI TGC'GCA Cuts at: 2300 4167 2300 Size: 1867 4519 HaeII r_GCGC'y Cuts at: 132 1421 2966 3820 4068 5090 6069 132 Size: 1289 1545 854 248 1022 979 449 Fragments arranged by size: 1545 1289 1022 979 854 449 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 482 502 514 534 566 598 1025 1057 1229 Size: 20 12 20 32 32 427 32 172 Cuts at: 1229 1261 1309 1341 3129 3161 3278 3310 4228 Size: 32 48 32 1788 32 117 32 918 Cuts at: 4228 4258 4260 4290 482 Size: 30 2 30 2578 Fragments arranged by size: 2578 1788 918 427 172 117 48 32 32 32 32 32 32 32 30 30 20 20 12 2 HincII GTy'rAC Cuts at: 823 3425 823 Size: 2602 3784 HindIII A'AGCT_T Cuts at: 2259 2259 Size: 6386 KasI G'GCGC_C Cuts at: 128 3816 4064 128 Size: 3688 248 2450 Fragments arranged by size: 3688 2450 248 KpnI G_GTAC'C Cuts at: 3406 3406 Size: 6386 MfeI C'AATT_G Cuts at: 1787 1787 Size: 6386 MluI A'CGCG_T Cuts at: 5819 5819 Size: 6386 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 379 785 2260 2840 5057 5241 379 Size: 406 1475 580 2217 184 1524 Fragments arranged by size: 2217 1524 1475 580 406 184 MscI TGG'CCA Cuts at: 685 1345 2139 2716 3190 4147 685 Size: 660 794 577 474 957 2924 Fragments arranged by size: 2924 957 794 660 577 474 MslI CAynn'nnrTG Cuts at: 268 541 619 865 871 1255 1412 2179 2407 Size: 273 78 246 6 384 157 767 228 Cuts at: 2407 2941 3025 3343 4375 4502 268 Size: 534 84 318 1032 127 2152 Fragments arranged by size: 2152 1032 767 534 384 318 273 246 228 157 127 84 78 6 MspA1I CmG'CkG Cuts at: 189 2336 3497 3811 4171 5184 5429 189 Size: 2147 1161 314 360 1013 245 1146 Fragments arranged by size: 2147 1161 1146 1013 360 314 245 NaeI GCC'GGC Cuts at: 854 4567 854 Size: 3713 2673 NarI GG'CG_CC Cuts at: 129 3817 4065 129 Size: 3688 248 2450 Fragments arranged by size: 3688 2450 248 NcoI C'CATG_G Cuts at: 464 866 1694 2519 2648 3023 3114 464 Size: 402 828 825 129 375 91 3736 Fragments arranged by size: 3736 828 825 402 375 129 91 NgoMIV G'CCGG_C Cuts at: 852 4565 852 Size: 3713 2673 NsiI A_TGCA'T Cuts at: 1102 1102 Size: 6386 NspI r_CATG'y Cuts at: 573 1050 1203 1254 2202 3012 3439 3939 4846 Size: 477 153 51 948 810 427 500 907 Cuts at: 4846 573 Size: 2113 Fragments arranged by size: 2113 948 907 810 500 477 427 153 51 PasI CC'CwG_GG Cuts at: 506 506 Size: 6386 PciI A'CATG_T Cuts at: 1199 2198 4842 1199 Size: 999 2644 2743 Fragments arranged by size: 2743 2644 999 PflMI CCAn_nnn'nTGG Cuts at: 2460 2460 Size: 6386 PfoI T'CCnGG_A Cuts at: 3215 3215 Size: 6386 PmeI GTTT'AAAC Cuts at: 3385 3385 Size: 6386 PmlI CAC'GTG Cuts at: 2697 3275 2697 Size: 578 5808 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5525 5557 5525 Size: 32 6354 PpuMI rG'GwC_Cy Cuts at: 1368 1455 3315 3353 1368 Size: 87 1860 38 4401 Fragments arranged by size: 4401 1860 87 38 PsiI TTA'TAA Cuts at: 1424 1424 Size: 6386 PspOMI G'GGCC_C Cuts at: 903 903 Size: 6386 PstI C_TGCA'G Cuts at: 902 1304 3260 3433 4118 902 Size: 402 1956 173 685 3170 Fragments arranged by size: 3170 1956 685 402 173 PvuI CG_AT'CG Cuts at: 104 104 Size: 6386 PvuII CAG'CTG Cuts at: 2336 3811 4171 2336 Size: 1475 360 4551 Fragments arranged by size: 4551 1475 360 RsrII CG'GwC_CG Cuts at: 1581 4581 1581 Size: 3000 3386 SacI G_AGCT'C Cuts at: 1061 3147 3400 1061 Size: 2086 253 4047 Fragments arranged by size: 4047 2086 253 SacII CC_GC'GG Cuts at: 190 190 Size: 6386 SalI G'TCGA_C Cuts at: 3423 3423 Size: 6386 SanDI GG'GwC_CC Cuts at: 1368 1455 1368 Size: 87 6299 SapI GCTCTTCn'nnn_ Cuts at: 758 4409 4619 758 Size: 3651 210 2525 Fragments arranged by size: 3651 2525 210 SbfI CC_TGCA'GG Cuts at: 3433 3433 Size: 6386 ScaI AGT'ACT Cuts at: 1868 5903 1868 Size: 4035 2351 SexAI A'CCwGG_T Cuts at: 512 665 1475 512 Size: 153 810 5423 Fragments arranged by size: 5423 810 153 SfcI C'TryA_G Cuts at: 33 898 1300 2331 2469 2589 3256 3429 4114 Size: 865 402 1031 138 120 667 173 685 Cuts at: 4114 5107 5298 33 Size: 993 191 1121 Fragments arranged by size: 1121 1031 993 865 685 667 402 191 173 138 120 SfoI GGC'GCC Cuts at: 130 3818 4066 130 Size: 3688 248 2450 Fragments arranged by size: 3688 2450 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6386 SmaI CCC'GGG Cuts at: 196 1031 3408 196 Size: 835 2377 3174 Fragments arranged by size: 3174 2377 835 SmlI C'TyrA_G Cuts at: 1725 1819 3541 4456 4948 5210 1725 Size: 94 1722 915 492 262 2901 Fragments arranged by size: 2901 1722 915 492 262 94 SpeI A'CTAG_T Cuts at: 67 1038 67 Size: 971 5415 SphI G_CATG'C Cuts at: 3439 3939 3439 Size: 500 5886 SspI AAT'ATT Cuts at: 2584 2584 Size: 6386 StyI C'CwwG_G Cuts at: 464 866 908 1340 1505 1694 2414 2519 2648 Size: 402 42 432 165 189 720 105 129 Cuts at: 2648 3023 3114 3520 3874 464 Size: 375 91 406 354 2976 Fragments arranged by size: 2976 720 432 406 402 375 354 189 165 129 105 91 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1569 2461 3969 4102 4561 4770 1569 Size: 892 1508 133 459 209 3185 Fragments arranged by size: 3185 1508 892 459 209 133 TatI w'GTAC_w Cuts at: 548 1866 3005 5901 548 Size: 1318 1139 2896 1033 Fragments arranged by size: 2896 1318 1139 1033 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 486 518 5903 5935 486 Size: 32 5385 32 937 Fragments arranged by size: 5385 937 32 32 Tth111I GACn'n_nGTC Cuts at: 2761 4183 2761 Size: 1422 4964 XbaI T'CTAG_A Cuts at: 3417 3417 Size: 6386 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1357 1555 3881 1357 Size: 198 2326 3862 Fragments arranged by size: 3862 2326 198 XmaI C'CCGG_G Cuts at: 194 1029 3406 194 Size: 835 2377 3174 Fragments arranged by size: 3174 2377 835 XmnI GAAnn'nnTTC Cuts at: 1832 2617 2843 1832 Size: 785 226 5375 Fragments arranged by size: 5375 785 226 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AhdI AloI AscI AvrII BbvCI BcgI BmtI Bpu10I BsiWI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI HpaI NdeI NheI NotI NruI PacI PshAI PspXI PsrI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI