Restriction map for pF1KE3836
##################################
# NO CUT ENZYMES (   32 enzymes) #
##################################

AclI        AscI        AseI        AsnI        AvrII       
BlnI        BsiWI       BssHII      Bst1107I    BstBI       
BstZ17I     DraI        EcoRV       HpaI        MfeI        
MluI        MunI        NotI        NruI        NspV        
PacI        PaeR7I      PmeI        PshBI       Psp1406I    
PvuI        ScaI        SfuI        SnaBI       SspI        
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AatII       12286  GACGTC               GACGT^C
Acc65I       9470  GGTACC               G^GTACC
AflII        9154  CTTAAG               C^TTAAG
AocI        13603  CCTGAGG              CC^T[ACGT]AGG
Asp718I      9470  GGTACC               G^GTACC
BclI         5406  TGATCA               T^GATCA
BfrI         9154  CTTAAG               C^TTAAG
BglII        3959  AGATCT               A^GATCT
BsmI        11677  GCATTC               GAATGC(1/-1)
Bsu36I      13603  CCTGAGG              CC^T[ACGT]AGG
Eco81I      13603  CCTGAGG              CC^T[ACGT]AGG
EcoT22I      3222  ATGCAT               ATGCA^T
FbaI         5406  TGATCA               T^GATCA
FseI         1597  GGCCGGCC             GGCCGG^CC
HindIII     12193  AAGCTT               A^AGCTT
KpnI         9470  GGTACC               GGTAC^C
NdeI          513  CATATG               CA^TATG
NheI        10006  GCTAGC               G^CTAGC
NsiI         3222  ATGCAT               ATGCA^T
SalI         1084  GTCGAC               G^TCGAC
SgrAI       11821  CACCGGCG             C[GA]^CCGG[CT]G
SpeI         3997  ACTAGT               A^CTAGT
XbaI         6018  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccIII       4130  TCCGGA               T^CCGGA
             5636  TCCGGA              
AgeI         2714  ACCGGT               A^CCGGT
             9104  ACCGGT              
AhdI         1543  GACGGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4354  GACGCCAGGTC         
Alw44I       4276  GTGCAC               G^TGCAC
             5616  GTGCAC              
ApaLI        4276  GTGCAC               G^TGCAC
             5616  GTGCAC              
AspEI        1543  GACGGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4354  GACGCCAGGTC         
BseAI        4130  TCCGGA               T^CCGGA
             5636  TCCGGA              
Bsp1407I     8438  TGTACA               T^GTACA
            11609  TGTACA              
BspEI        4130  TCCGGA               T^CCGGA
             5636  TCCGGA              
BsrGI        8438  TGTACA               T^GTACA
            11609  TGTACA              
CpoI         2690  CGGACCG              CG^G[AT]CCG
             4675  CGGACCG             
Eam1105I     1543  GACGGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4354  GACGCCAGGTC         
EcoNI        3598  CCTGGCGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             9714  CCTGTTTGAGG         
EcoRI        5708  GAATTC               G^AATTC
             7560  GAATTC              
KspI         3966  CCGCGG               CCGC^GG
             9039  CCGCGG              
MroI         4130  TCCGGA               T^CCGGA
             5636  TCCGGA              
PinAI        2714  ACCGGT               A^CCGGT
             9104  ACCGGT              
RsrII        2690  CGGACCG              CG^G[AT]CCG
             4675  CGGACCG             
SacII        3966  CCGCGG               CCGC^GG
             9039  CCGCGG              
SbfI         3431  CCTGCAGG             CCTGCA^GG
             4572  CCTGCAGG            
SfiI         5218  GGCCAGAAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             5227  GGCCCCGTGGGCC       
SphI          779  GCATGC               GCATG^C
            12755  GCATGC              
Sse8387I     3431  CCTGCAGG             CCTGCA^GG
             4572  CCTGCAGG            
SspBI        8438  TGTACA               T^GTACA
            11609  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  179 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
Aor51HI     ApaI        ApoI        Asp700I     AspHI       
AspI        AvaI        AvaII       AviII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspDI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        DdeI        
DpnII       DraII       DraIII      DrdI        DsaI        
EaeI        EagI        EarI        EclXI       Eco47III    
Eco52I      EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflFI       PflMI       PleI        PmaCI       PmlI        
PpuMI       PshAI       PstI        PvuII       RcaI        
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SfoI        
SmaI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        XmnI        
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