Restriction map for pF1KE3836
 (Linear) (Six-base) MAPSORT of: KIEE3836.seq  Check: 6523  from: 1  to: 13632



KIEE3836.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     403     484    2689    2812    2932    3667    4566    4738 
   Size:        403      81    2205     123     120     735     899     172

Cuts at:   4738    7555   11506   11584   11799   12798   12850   13093   13632 
   Size:       2817    3951      78     215     999      52     243     539

  Fragments arranged by size: 

                3951    2817    2205     999     899     735     539     403
                 243     215     172     123     120      81      78      52
            
 
AatII G_ACGT'C

Cuts at:      0   12290   13632 
   Size:      12290    1342
 
AccI GT'mk_AC

Cuts at:      0     584     658    1085    1142    2294    4052    4472    9731 
   Size:        584      74     427      57    1152    1758     420    5259

Cuts at:   9731   13632 
   Size:       3901

  Fragments arranged by size: 

                5259    3901    1758    1152     584     427     420      74
                  57
 
Acc65I G'GTAC_C

Cuts at:      0    9470   13632 
   Size:       9470    4162
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406     687    1512    1810    5196    6584    6753    7602 
   Size:        406     281     825     298    3386    1388     169     849

Cuts at:   7602    8938   11890   12261   13221   13632 
   Size:       1336    2952     371     960     411

  Fragments arranged by size: 

                3386    2952    1388    1336     960     849     825     411
                 406     371     298     281     169
 
AfeI AGC'GCT

Cuts at:      0    2608    8371    8983   11257   13632 
   Size:       2608    5763     612    2274    2375

  Fragments arranged by size: 

                5763    2608    2375    2274     612
 
AflII C'TTAA_G

Cuts at:      0    9154   13632 
   Size:       9154    4478
 
AflIII A'CryG_T

Cuts at:      0     937    2860    6290    6470    8140    8354    8935    9146 
   Size:        937    1923    3430     180    1670     214     581     211

Cuts at:   9146   11285   11912   13632 
   Size:       2139     627    1720

  Fragments arranged by size: 

                3430    2139    1923    1720    1670     937     627     581
                 214     211     180
 
AgeI A'CCGG_T

Cuts at:      0    2714    9104   13632 
   Size:       2714    6390    4528

  Fragments arranged by size: 

                6390    4528    2714
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1548    4359   13632 
   Size:       1548    2811    9273

  Fragments arranged by size: 

                9273    2811    1548
 
AleI CACnn'nnGTG

Cuts at:      0     368    1363    2556    3452    4942    5485    6469    6832 
   Size:        368     995    1193     896    1490     543     984     363

Cuts at:   6832    8353    8519    9941    9988   10948   12544   13632 
   Size:       1521     166    1422      47     960    1596    1088

  Fragments arranged by size: 

                1596    1521    1490    1422    1193    1088     995     984
                 960     896     543     368     363     166      47
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3247    3470    4460    8448    8784    9719    9959   10897 
   Size:       3247     223     990    3988     336     935     240     938

Cuts at:  10897   10966   12562   12830   13195   13632 
   Size:         69    1596     268     365     437

  Fragments arranged by size: 

                3988    3247    1596     990     938     935     437     365
                 336     268     240     223      69
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3406    3438   13632 
   Size:       3406      32   10194

  Fragments arranged by size: 

               10194    3406      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2594    5100    6304    6504    7255    7473    7868    8253 
   Size:       2594    2506    1204     200     751     218     395     385

Cuts at:   8253    8730    8852    9165   11372   11607   12842   13037   13632 
   Size:        477     122     313    2207     235    1235     195     595

  Fragments arranged by size: 

                2594    2506    2207    1235    1204     751     595     477
                 395     385     313     235     218     200     195     122
            
 
ApaI G_GGCC'C

Cuts at:      0     317     392     651    1868    3647    4642    5230   12793 
   Size:        317      75     259    1217    1779     995     588    7563

Cuts at:  12793   13217   13603   13632 
   Size:        424     386      29

  Fragments arranged by size: 

                7563    1779    1217     995     588     424     386     317
                 259      75      29
 
ApaLI G'TGCA_C

Cuts at:      0    4276    5616   13632 
   Size:       4276    1340    8016

  Fragments arranged by size: 

                8016    4276    1340
 
ApoI r'AATT_y

Cuts at:      0    5708    7560    9640   13632 
   Size:       5708    1852    2080    3992

  Fragments arranged by size: 

                5708    3992    2080    1852
 
AvaI C'yCGr_G

Cuts at:      0    1361    1412    1682    3085    4036    4187    5451    6029 
   Size:       1361      51     270    1403     951     151    1264     578

Cuts at:   6029    7089    8365    8551    9925   10345   10403   11059   11430 
   Size:       1060    1276     186    1374     420      58     656     371

Cuts at:  11430   11928   11988   13632 
   Size:        498      60    1644

  Fragments arranged by size: 

                1644    1403    1374    1361    1276    1264    1060     951
                 656     578     498     420     371     270     186     151
                  60      58      51
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    7113    7146   13632 
   Size:       7113      33    6486

  Fragments arranged by size: 

                7113    6486      33
 
BamHI G'GATC_C

Cuts at:      0    1965    4182    4196    5718   13632 
   Size:       1965    2217      14    1522    7914

  Fragments arranged by size: 

                7914    2217    1965    1522      14
 
BanI G'GyrC_C

Cuts at:      0     472    1378    2132    2407    3594    3876    4265    4830 
   Size:        472     906     754     275    1187     282     389     565

Cuts at:   4830    5536    6463    6494    6736    6748    8023    8347    9470 
   Size:        706     927      31     242      12    1275     324    1123

Cuts at:   9470    9784   11138   11494   11764   12730   12770   13632 
   Size:        314    1354     356     270     966      40     862

  Fragments arranged by size: 

                1354    1275    1187    1123     966     927     906     862
                 754     706     565     472     389     356     324     314
                 282     275     270     242      40      31      12
 
BanII G_rGCy'C

Cuts at:      0     317     332     392     651    1689    1868    3542    3647 
   Size:        317      15      60     259    1038     179    1674     105

Cuts at:   3647    3993    4316    4642    5230    5458    6850    8617    9440 
   Size:        346     323     326     588     228    1392    1767     823

Cuts at:   9440    9447    9771   10097   10457   10580   11198   12008   12490 
   Size:          7     324     326     360     123     618     810     482

Cuts at:  12490   12793   13217   13603   13632 
   Size:        303     424     386      29

  Fragments arranged by size: 

                1767    1674    1392    1038     823     810     618     588
                 482     424     386     360     346     326     326     324
                 323     317     303     259     228     179     123     105
                  60      29      15       7
 
BbeI G_GCGC'C

Cuts at:      0    2136    4269    8027   11142   13632 
   Size:       2136    2133    3758    3115    2490

  Fragments arranged by size: 

                3758    3115    2490    2136    2133
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1312    5876    6432    6572    7708    9831   11569   12327 
   Size:       1312    4564     556     140    1136    2123    1738     758

Cuts at:  12327   13227   13632 
   Size:        900     405

  Fragments arranged by size: 

                4564    2123    1738    1312    1136     900     758     556
                 405     140
 
BbvCI CC'TCA_GC

Cuts at:      0    2489    3916    8756    9301   13632 
   Size:       2489    1427    4840     545    4331

  Fragments arranged by size: 

                4840    4331    2489    1427     545
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1062    1096    2684    2718    3879    3913    7141    7175 
   Size:       1062      34    1588      34    1161      34    3228      34

Cuts at:   7175    9528    9562   11050   11084   11271   11305   11874   11908 
   Size:       2353      34    1488      34     187      34     569      34

Cuts at:  11908   13632 
   Size:       1724

  Fragments arranged by size: 

                3228    2353    1724    1588    1488    1161    1062     569
                 187      34      34      34      34      34      34      34
                  34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     526    6098    6643   13632 
   Size:        526    5572     545    6989

  Fragments arranged by size: 

                6989    5572     545     526
 
BclI T'GATC_A

Cuts at:      0    5406   13632 
   Size:       5406    8226
 
BfrBI ATG'CAT

Cuts at:      0    3224   13632 
   Size:       3224   10408
 
BglI GCCn_nnn'nGGC

Cuts at:      0     794    1523    1604    2106    2567    3629    4311    5225 
   Size:        794     729      81     502     461    1062     682     914

Cuts at:   5225    5234    5357    7520    9518    9656   11432   12779   12887 
   Size:          9     123    2163    1998     138    1776    1347     108

Cuts at:  12887   13632 
   Size:        745

  Fragments arranged by size: 

                2163    1998    1776    1347    1062     914     794     745
                 729     682     502     461     138     123     108      81
                   9
 
BglII A'GATC_T

Cuts at:      0    3959   13632 
   Size:       3959    9673
 
BlpI GC'TnA_GC

Cuts at:      0    2110    9679   12484   13632 
   Size:       2110    7569    2805    1148

  Fragments arranged by size: 

                7569    2805    2110    1148
 
Bme1580I G_kGCm'C

Cuts at:      0     317     392     651     777    1383    1868    3647    3881 
   Size:        317      75     259     126     606     485    1779     234

Cuts at:   3881    4280    4642    4835    5230    5431    5539    5620    6398 
   Size:        399     362     193     395     201     108      81     778

Cuts at:   6398    6549    6751    9869    9938   10649   11073   11086   11450 
   Size:        151     202    3118      69     711     424      13     364

Cuts at:  11450   11767   12557   12733   12775   12793   13198   13217   13603 
   Size:        317     790     176      42      18     405      19     386

Cuts at:  13603   13632 
   Size:         29

  Fragments arranged by size: 

                3118    1779     790     778     711     606     485     424
                 405     399     395     386     364     362     317     317
                 259     234     202     201     193     176     151     126
                 108      81      75      69      42      29      19      18
                  13
 
BmgBI CAC'GTC

Cuts at:      0    2861    3306    3945    4765    4899    5591    5861    5874 
   Size:       2861     445     639     820     134     692     270      13

Cuts at:   5874    8141    8318    8936    9864   10233   12405   13233   13632 
   Size:       2267     177     618     928     369    2172     828     399

  Fragments arranged by size: 

                2861    2267    2172     928     828     820     692     639
                 618     445     399     369     270     177     134      13
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1155    2007    2151    2550    3048    3311    4086    4224 
   Size:       1155     852     144     399     498     263     775     138

Cuts at:   4224    5007    5743    5820    7317    8205    8573    9339    9498 
   Size:        783     736      77    1497     888     368     766     159

Cuts at:   9498    9621    9753   10238   11907   12066   12189   12485   12864 
   Size:        123     132     485    1669     159     123     296     379

Cuts at:  12864   13632 
   Size:        768

  Fragments arranged by size: 

                1669    1497    1155     888     852     783     775     768
                 766     736     498     485     399     379     368     296
                 263     159     159     144     138     132     123     123
                  77
 
BmtI G_CTAG'C

Cuts at:      0   10010   13632 
   Size:      10010    3622
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3417    4263    4308    7914    8004   13632 
   Size:       3417     846      45    3606      90    5628

  Fragments arranged by size: 

                5628    3606    3417     846      90      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1915    2056    3000    3255    4351    5122    5347    6262 
   Size:       1915     141     944     255    1096     771     225     915

Cuts at:   6262    6348    6801    7007    7516    7845   13632 
   Size:         86     453     206     509     329    5787

  Fragments arranged by size: 

                5787    1915    1096     944     915     771     509     453
                 329     255     225     206     141      86
 
Bpu10I CC'TnA_GC

Cuts at:      0    2219    2489    3916    4505    5827    6689    8756    9301 
   Size:       2219     270    1427     589    1322     862    2067     545

Cuts at:   9301   12388   13352   13632 
   Size:       3087     964     280

  Fragments arranged by size: 

                3087    2219    2067    1427    1322     964     862     589
                 545     280     270
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751    6610    8860   13632 
   Size:        751    5859    2250    4772

  Fragments arranged by size: 

                5859    4772    2250     751
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     712    1699    1774    4279    5007    6110    6961    8173 
   Size:        712     987      75    2505     728    1103     851    1212

Cuts at:   8173   10781   13632 
   Size:       2608    2851

  Fragments arranged by size: 

                2851    2608    2505    1212    1103     987     851     728
                 712      75
 
BsaAI yAC'GTr

Cuts at:      0     635     843    2544    6000    7344    8355   12764   13632 
   Size:        635     208    1701    3456    1344    1011    4409     868

  Fragments arranged by size: 

                4409    3456    1701    1344    1011     868     635     208
            
 
BsaBI GATnn'nnATC

Cuts at:      0     856    1157    4774   13632 
   Size:        856     301    3617    8858

  Fragments arranged by size: 

                8858    3617     856     301
 
BsaHI Gr'CG_yC

Cuts at:      0      65    1404    2133    2516    4266    4355    6416    7184 
   Size:         65    1339     729     383    1750      89    2061     768

Cuts at:   7184    7637    7895    8024    9620   10904   11139   12188   12287 
   Size:        453     258     129    1596    1284     235    1049      99

Cuts at:  12287   13632 
   Size:       1345

  Fragments arranged by size: 

                2061    1750    1596    1345    1339    1284    1049     768
                 729     453     383     258     235     129      99      89
                  65
 
BsaWI w'CCGG_w

Cuts at:      0    2195    2714    4130    4673    5636    9104   13632 
   Size:       2195     519    1416     543     963    3468    4528

  Fragments arranged by size: 

                4528    3468    2195    1416     963     543     519
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2029    2059    2274    2304    2806    2836    4573    4603 
   Size:       2029      30     215      30     502      30    1737      30

Cuts at:   4603    4804    4834    4987    5017    6043    6073    6799    6829 
   Size:        201      30     153      30    1026      30     726      30

Cuts at:   6829    6832    6862    7085    7115    7669    7699   11227   11257 
   Size:          3      30     223      30     554      30    3528      30

Cuts at:  11257   13632 
   Size:       2375

  Fragments arranged by size: 

                3528    2375    2029    1737    1026     726     554     502
                 223     215     201     153      30      30      30      30
                  30      30      30      30      30      30      30      30
                   3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1434    2017    3424    8319    9790   10945   11077   11256 
   Size:       1434     583    1407    4895    1471    1155     132     179

Cuts at:  11256   11259   11332   13240   13632 
   Size:          3      73    1908     392

  Fragments arranged by size: 

                4895    1908    1471    1434    1407    1155     583     392
                 179     132      73       3
 
BseYI C'CCAG_C

Cuts at:      0     206     728    1298    2128    2390    2658    3639    3752 
   Size:        206     522     570     830     262     268     981     113

Cuts at:   3752    4261    4559    5355    5573    6878    7066    7382    8005 
   Size:        509     298     796     218    1305     188     316     623

Cuts at:   8005    8009   10524   11504   12707   12803   13114   13632 
   Size:          4    2515     980    1203      96     311     518

  Fragments arranged by size: 

                2515    1305    1203     981     980     830     796     623
                 570     522     518     509     316     311     298     268
                 262     218     206     188     113      96       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     820    3135    5113    6691    7345    7737    8429    8549 
   Size:        820    2315    1978    1578     654     392     692     120

Cuts at:   8549    8673    8754    8976    9298    9481    9799    9984   10140 
   Size:        124      81     222     322     183     318     185     156

Cuts at:  10140   10449   10692   10809   12139   12489   12828   12972   13050 
   Size:        309     243     117    1330     350     339     144      78

Cuts at:  13050   13077   13632 
   Size:         27     555

  Fragments arranged by size: 

                2315    1978    1578    1330     820     692     654     555
                 392     350     339     322     318     309     243     222
                 185     183     156     144     124     120     117      81
                  78      27
 
BsiEI CG_ry'CG

Cuts at:      0    2163    8460    8520    8931    9513   10422   10653   10755 
   Size:       2163    6297      60     411     582     909     231     102

Cuts at:  10755   12903   13632 
   Size:       2148     729

  Fragments arranged by size: 

                6297    2163    2148     909     729     582     411     231
                 102      60
 
BsiHKAI G_wGCw'C

Cuts at:      0     332     463     679     812    1235    1652    3092    3542 
   Size:        332     131     216     133     423     417    1440     450

Cuts at:   3542    3556    3739    4163    4280    5620    5833    6695    7292 
   Size:         14     183     424     117    1340     213     862     597

Cuts at:   7292    7578    7967    8617    8705   10144   10381   12716   12925 
   Size:        286     389     650      88    1439     237    2335     209

Cuts at:  12925   13232   13632 
   Size:        307     400

  Fragments arranged by size: 

                2335    1440    1439    1340     862     650     597     450
                 424     423     417     400     389     332     307     286
                 237     216     213     209     183     133     131     117
                  88      14
 
BsmI GAATG_Cn'

Cuts at:      0   11677   13632 
   Size:      11677    1955
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2164    4962    5590   12073   13632 
   Size:       2164    2798     628    6483    1559

  Fragments arranged by size: 

                6483    2798    2164    1559     628
 
Bsp1286I G_dGCh'C

Cuts at:      0     317     332     392     463     651     679     777     812 
   Size:        317      15      60      71     188      28      98      35

Cuts at:    812    1235    1383    1652    1689    1868    3092    3542    3556 
   Size:        423     148     269      37     179    1224     450      14

Cuts at:   3556    3647    3739    3881    3993    4163    4280    4316    4642 
   Size:         91      92     142     112     170     117      36     326

Cuts at:   4642    4835    5230    5431    5458    5539    5620    5833    6398 
   Size:        193     395     201      27      81      81     213     565

Cuts at:   6398    6549    6695    6751    6850    7292    7578    7967    8617 
   Size:        151     146      56      99     442     286     389     650

Cuts at:   8617    8705    9440    9447    9771    9869    9938   10097   10144 
   Size:         88     735       7     324      98      69     159      47

Cuts at:  10144   10381   10457   10580   10649   11073   11086   11198   11450 
   Size:        237      76     123      69     424      13     112     252

Cuts at:  11450   11767   12008   12490   12557   12716   12733   12775   12793 
   Size:        317     241     482      67     159      17      42      18

Cuts at:  12793   12925   13198   13217   13232   13603   13632 
   Size:        132     273      19      15     371      29

  Fragments arranged by size: 

                1224     735     650     565     482     450     442     424
                 423     395     389     371     326     324     317     317
                 286     273     269     252     241     237     213     201
                 193     188     179     170     159     159     151     148
                 146     142     132     123     117     112     112      99
                  98      98      92      91      88      81      81      76
                  71      69      69      67      60      56      47      42
                  37      36      35      29      28      27      19      18
                  17      15      15      14      13       7
 
BspEI T'CCGG_A

Cuts at:      0    4130    5636   13632 
   Size:       4130    1506    7996

  Fragments arranged by size: 

                7996    4130    1506
 
BspHI T'CATG_A

Cuts at:      0    1673    4703   11558   13632 
   Size:       1673    3030    6855    2074

  Fragments arranged by size: 

                6855    3030    2074    1673
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     403     448     484    1516    2689    2812    2932    2956 
   Size:        403      45      36    1032    1173     123     120      24

Cuts at:   2956    3106    3667    4566    4580    4738    6608    7555    7636 
   Size:        150     561     899      14     158    1870     947      81

Cuts at:   7636    7855    8578    9122    9270    9839   10363   10966   11506 
   Size:        219     723     544     148     569     524     603     540

Cuts at:  11506   11584   11799   12611   12798   12850   13093   13135   13632 
   Size:         78     215     812     187      52     243      42     497

  Fragments arranged by size: 

                1870    1173    1032     947     899     812     723     603
                 569     561     544     540     524     497     403     243
                 219     215     187     158     150     148     123     120
                  81      78      52      45      42      36      24      14
            
 
BsrBI CCG'CTC

Cuts at:      0     557    1776    2355    2891    5606    6102    7206    7555 
   Size:        557    1219     579     536    2715     496    1104     349

Cuts at:   7555    7983   10865   11429   12171   13632 
   Size:        428    2882     564     742    1461

  Fragments arranged by size: 

                2882    2715    1461    1219    1104     742     579     564
                 557     536     496     428     349
 
BsrDI GCAATG_nn'

Cuts at:      0     282     946     980    5906    7728    8718    9279   10305 
   Size:        282     664      34    4926    1822     990     561    1026

Cuts at:  10305   12729   13152   13632 
   Size:       2424     423     480

  Fragments arranged by size: 

                4926    2424    1822    1026     990     664     561     480
                 423     282      34
 
BsrFI r'CCGG_y

Cuts at:      0     587    1598    1708    2714    3025    3206    4147    4288 
   Size:        587    1011     110    1006     311     181     941     141

Cuts at:   4288    4522    5623    6406    6452    6592    9104    9818   10121 
   Size:        234    1101     783      46     140    2512     714     303

Cuts at:  10121   11093   11822   12767   12914   13632 
   Size:        972     729     945     147     718

  Fragments arranged by size: 

                2512    1101    1011    1006     972     945     941     783
                 729     718     714     587     311     303     234     181
                 147     141     140     110      46
 
BsrGI T'GTAC_A

Cuts at:      0    8438   11609   13632 
   Size:       8438    3171    2023

  Fragments arranged by size: 

                8438    3171    2023
 
BssSI C'ACGA_G

Cuts at:      0    1582    3165    4423   13632 
   Size:       1582    1583    1258    9209

  Fragments arranged by size: 

                9209    1583    1582    1258
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     440    2450    8318    9911   12464   13632 
   Size:        440    2010    5868    1593    2553    1168

  Fragments arranged by size: 

                5868    2553    2010    1593    1168     440
 
BstEII G'GTnAC_C

Cuts at:      0    5877    9493   10350   13632 
   Size:       5877    3616     857    3282

  Fragments arranged by size: 

                5877    3616    3282     857
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     696    2236    3903    4143    5373    7143    9447   10532 
   Size:        696    1540    1667     240    1230    1770    2304    1085

Cuts at:  10532   11149   12021   12147   12352   13395   13632 
   Size:        617     872     126     205    1043     237

  Fragments arranged by size: 

                2304    1770    1667    1540    1230    1085    1043     872
                 696     617     240     237     205     126
 
BstYI r'GATC_y

Cuts at:      0     166     908    1031    1965    2372    3665    3959    4182 
   Size:        166     742     123     934     407    1293     294     223

Cuts at:   4182    4196    4775    5718    7790   11630   12341   13632 
   Size:         14     579     943    2072    3840     711    1291

  Fragments arranged by size: 

                3840    2072    1293    1291     943     934     742     711
                 579     407     294     223     166     123      14
 
Bsu36I CC'TnA_GG

Cuts at:      0   13604   13632 
   Size:      13604      28
 
BtgI C'CryG_G

Cuts at:      0     695     998    1823    2250    3966    4319    4538    4601 
   Size:        695     303     825     427    1716     353     219      63

Cuts at:   4601    4980    5112    5231    5459    6522    6549    6851    6873 
   Size:        379     132     119     228    1063      27     302      22

Cuts at:   6873    7000    7391    7406    8997    9039    9824   12152   12776 
   Size:        127     391      15    1591      42     785    2328     624

Cuts at:  12776   13542   13632 
   Size:        766      90

  Fragments arranged by size: 

                2328    1716    1591    1063     825     785     766     695
                 624     427     391     379     353     303     302     228
                 219     132     127     119      90      63      42      27
                  22      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     225    3116    3260    3290    3882    6221    6693    7386 
   Size:        225    2891     144      30     592    2339     472     693

Cuts at:   7386    8215    8282    8406    9989   10193   10746   10796   11033 
   Size:        829      67     124    1583     204     553      50     237

Cuts at:  11033   11162   11598   11871   12103   13632 
   Size:        129     436     273     232    1529

  Fragments arranged by size: 

                2891    2339    1583    1529     829     693     592     553
                 472     436     273     237     232     225     204     144
                 129     124      67      50      30
 
BtsI GCAGTG_nn'

Cuts at:      0     318    1545    2295    2355    4454    5093    5556    5918 
   Size:        318    1227     750      60    2099     639     463     362

Cuts at:   5918    7328    7644    7720    7923    8184    8254    8685    8745 
   Size:       1410     316      76     203     261      70     431      60

Cuts at:   8745    8761   10452   10666   10869   11217   12613   12925   12989 
   Size:         16    1691     214     203     348    1396     312      64

Cuts at:  12989   13137   13632 
   Size:        148     495

  Fragments arranged by size: 

                2099    1691    1410    1396    1227     750     639     495
                 463     431     362     348     318     316     312     261
                 214     203     203     148      76      70      64      60
                  60      16
 
ClaI AT'CG_AT

Cuts at:      0     899    3845    5336   13632 
   Size:        899    2946    1491    8296

  Fragments arranged by size: 

                8296    2946    1491     899
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1865    1900    2739    2774    2985    3020    7440    7475 
   Size:       1865      35     839      35     211      35    4420      35

Cuts at:   7475    8016    8051    8829    8864    9876    9911   13053   13088 
   Size:        541      35     778      35    1012      35    3142      35

Cuts at:  13088   13632 
   Size:        544

  Fragments arranged by size: 

                4420    3142    1865    1012     839     778     544     541
                 211      35      35      35      35      35      35      35
                  35
 
DraIII CAC_nnn'GTG

Cuts at:      0     843    1809    6933    8505    9675   10994   11445   13632 
   Size:        843     966    5124    1572    1170    1319     451    2187

  Fragments arranged by size: 

                5124    2187    1572    1319    1170     966     843     451
            
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     940    2074    3868    6436    9835   10231   11695   11842 
   Size:        940    1134    1794    2568    3399     396    1464     147

Cuts at:  11842   13632 
   Size:       1790

  Fragments arranged by size: 

                3399    2568    1794    1790    1464    1134     940     396
                 147
 
EaeI y'GGCC_r

Cuts at:      0     629     854    1484    1600    1749    1820    1886    2456 
   Size:        629     225     630     116     149      71      66     570

Cuts at:   2456    2835    4028    4109    4145    4398    4524    5177    5217 
   Size:        379    1193      81      36     253     126     653      40

Cuts at:   5217    5375    5643    5892    6482    7145    7409    8775    8928 
   Size:        158     268     249     590     663     264    1366     153

Cuts at:   8928    9510   10650   10752   10866   11451   11529   11649   11733 
   Size:        582    1140     102     114     585      78     120      84

Cuts at:  11733   11903   12149   12354   13101   13134   13310   13562   13632 
   Size:        170     246     205     747      33     176     252      70

  Fragments arranged by size: 

                1366    1193    1140     747     663     653     630     629
                 590     585     582     570     379     268     264     253
                 252     249     246     225     205     176     170     158
                 153     149     126     120     116     114     102      84
                  81      78      71      70      66      40      36      33
            
 
EagI C'GGCC_G

Cuts at:      0    8928    9510   10650   10752   13632 
   Size:       8928     582    1140     102    2880

  Fragments arranged by size: 

                8928    2880    1140     582     102
 
EarI CTCTTCn'nnn_

Cuts at:      0    2269    3277    5215    7972   10580   10694   11177   13632 
   Size:       2269    1008    1938    2757    2608     114     483    2455

  Fragments arranged by size: 

                2757    2608    2455    2269    1938    1008     483     114
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    7933   13448   13632 
   Size:       7933    5515     184

  Fragments arranged by size: 

                7933    5515     184
 
EcoICRI GAG'CTC

Cuts at:      0     330    3540    8615   13632 
   Size:        330    3210    5075    5017

  Fragments arranged by size: 

                5075    5017    3210     330
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406     687    1512    1810    1915    2056    3000    3255 
   Size:        406     281     825     298     105     141     944     255

Cuts at:   3255    4351    5122    5196    5347    6262    6348    6584    6753 
   Size:       1096     771      74     151     915      86     236     169

Cuts at:   6753    6801    7007    7516    7602    7845    8938   11890   12261 
   Size:         48     206     509      86     243    1093    2952     371

Cuts at:  12261   13221   13632 
   Size:        960     411

  Fragments arranged by size: 

                2952    1096    1093     960     944     915     825     771
                 509     411     406     371     298     281     255     243
                 236     206     169     151     141     105      86      86
                  74      48
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3602    9718   13632 
   Size:       3602    6116    3914

  Fragments arranged by size: 

                6116    3914    3602
 
EcoO109I rG'GnC_Cy

Cuts at:      0     177     313     314     647    1638    2007    2215    2655 
   Size:        177     136       1     333     991     369     208     440

Cuts at:   2655    3170    3643    3756    4041    4124    4638    4639    5097 
   Size:        515     473     113     285      83     514       1     458

Cuts at:   5097    5226    5227    5759    6684    6953    7470    7844    8871 
   Size:        129       1     532     925     269     517     374    1027

Cuts at:   8871    9162    9282    9398   11604   12384   12790   13049   13213 
   Size:        291     120     116    2206     780     406     259     164

Cuts at:  13213   13214   13599   13600   13620   13632 
   Size:          1     385       1      20      12

  Fragments arranged by size: 

                2206    1027     991     925     780     532     517     515
                 514     473     458     440     406     385     374     369
                 333     291     285     269     259     208     177     164
                 136     129     120     116     113      83      20      12
                   1       1       1       1       1
 
EcoRI G'AATT_C

Cuts at:      0    5708    7560   13632 
   Size:       5708    1852    6072

  Fragments arranged by size: 

                6072    5708    1852
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4941    7191    8901   13632 
   Size:       4941    2250    1710    4731

  Fragments arranged by size: 

                4941    4731    2250    1710
 
FseI GG_CCGG'CC

Cuts at:      0    1602   13632 
   Size:       1602   12030
 
FspI TGC'GCA

Cuts at:      0    2347    3889    9118   11398   13632 
   Size:       2347    1542    5229    2280    2234

  Fragments arranged by size: 

                5229    2347    2280    2234    1542
 
FspAI rTGC'GCAy

Cuts at:      0   11398   13632 
   Size:      11398    2234
 
HaeII r_GCGC'y

Cuts at:      0    2136    2610    3243    3929    4269    7087    7731    8027 
   Size:       2136     474     633     686     340    2818     644     296

Cuts at:   8027    8121    8373    8607    8748    8985   10773   10893   11142 
   Size:         94     252     234     141     237    1788     120     249

Cuts at:  11142   11259   13632 
   Size:        117    2373

  Fragments arranged by size: 

                2818    2373    2136    1788     686     644     633     474
                 340     296     252     249     237     234     141     120
                 117      94
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477     541     573    1534    1566    2262    2294 
   Size:        445      32      64      32     961      32     696      32

Cuts at:   2294    2850    2882    3209    3241    3538    3570    4345    4377 
   Size:        556      32     327      32     297      32     775      32

Cuts at:   4377    4417    4449    4987    5019    5398    5430    6559    6591 
   Size:         40      32     538      32     379      32    1129      32

Cuts at:   6591    6799    6831    7085    7117    7657    7689   13632 
   Size:        208      32     254      32     540      32    5943

  Fragments arranged by size: 

                5943    1129     961     775     696     556     540     538
                 445     379     327     297     254     208      64      40
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1086    5556    6141    7503    9888   12372   12852   13062 
   Size:       1086    4470     585    1362    2385    2484     480     210

Cuts at:  13062   13632 
   Size:        570

  Fragments arranged by size: 

                4470    2484    2385    1362    1086     585     570     480
                 210
 
HindIII A'AGCT_T

Cuts at:      0   12193   13632 
   Size:      12193    1439
 
KasI G'GCGC_C

Cuts at:      0    2132    4265    8023   11138   13632 
   Size:       2132    2133    3758    3115    2494

  Fragments arranged by size: 

                3758    3115    2494    2133    2132
 
KpnI G_GTAC'C

Cuts at:      0    9474   13632 
   Size:       9474    4158
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2483    3845    5396    6623    6819    7763    9164   10019 
   Size:       2483    1362    1551    1227     196     944    1401     855

Cuts at:  10019   10513   11837   12586   13632 
   Size:        494    1324     749    1046

  Fragments arranged by size: 

                2483    1551    1401    1362    1324    1227    1046     944
                 855     749     494     196
 
MscI TGG'CCA

Cuts at:      0     631     856    1486    1888    2458    4030    4111    4400 
   Size:        631     225     630     402     570    1572      81     289

Cuts at:   4400    5179    5219    5377    5645    6484    7147    7411   12356 
   Size:        779      40     158     268     839     663     264    4945

Cuts at:  12356   13564   13632 
   Size:       1208      68

  Fragments arranged by size: 

                4945    1572    1208     839     779     663     631     630
                 570     402     289     268     264     225     158      81
                  68      40
 
MslI CAynn'nnrTG

Cuts at:      0     368     694    1363    1430    1624    2556    3191    3452 
   Size:        368     326     669      67     194     932     635     261

Cuts at:   3452    4408    4942    5173    5485    5911    6172    6289    6469 
   Size:        956     534     231     312     426     261     117     180

Cuts at:   6469    6832    6979    7232    7405    8128    8353    8519    8822 
   Size:        363     147     253     173     723     225     166     303

Cuts at:   8822    9379    9520    9941    9988   10471   10948   12019   12544 
   Size:        557     141     421      47     483     477    1071     525

Cuts at:  12544   13393   13632 
   Size:        849     239

  Fragments arranged by size: 

                1071     956     932     849     723     669     635     557
                 534     525     483     477     426     421     368     363
                 326     312     303     261     261     253     239     231
                 225     194     180     173     166     147     141     117
                  67      47
 
MspA1I CmG'CkG

Cuts at:      0    1514    2594    2732    2840    3071    3968    5303    5355 
   Size:       1514    1080     138     108     231     897    1335      52

Cuts at:   5355    5523    6155    6504    6531    7329    7427    7694    7832 
   Size:        168     632     349      27     798      98     267     138

Cuts at:   7832    7868    8009    8428    8591    8897    9041    9059   10274 
   Size:         36     141     419     163     306     144      18    1215

Cuts at:  10274   10295   10499   10517   10625   10640   10655   10757   10835 
   Size:         21     204      18     108      15      15     102      78

Cuts at:  10835   11004   11351   11543   12626   12662   12905   12986   13037 
   Size:        169     347     192    1083      36     243      81      51

Cuts at:  13037   13118   13632 
   Size:         81     514

  Fragments arranged by size: 

                1514    1335    1215    1083    1080     897     798     632
                 514     419     349     347     306     267     243     231
                 204     192     169     168     163     144     141     138
                 138     108     108     102      98      81      81      78
                  52      51      36      36      27      21      18      18
                  15      15
 
NaeI GCC'GGC

Cuts at:      0    1600    3027    3208    5625    6594   10123   13632 
   Size:       1600    1427     181    2417     969    3529    3509

  Fragments arranged by size: 

                3529    3509    2417    1600    1427     969     181
 
NarI GG'CG_CC

Cuts at:      0    2133    4266    8024   11139   13632 
   Size:       2133    2133    3758    3115    2493

  Fragments arranged by size: 

                3758    3115    2493    2133    2133
 
NcoI C'CATG_G

Cuts at:      0     695     998    2250    4601    5112    7391    8997    9824 
   Size:        695     303    1252    2351     511    2279    1606     827

Cuts at:   9824   13542   13632 
   Size:       3718      90

  Fragments arranged by size: 

                3718    2351    2279    1606    1252     827     695     511
                 303      90
 
NdeI CA'TA_TG

Cuts at:      0     514   13632 
   Size:        514   13118
 
NgoMIV G'CCGG_C

Cuts at:      0    1598    3025    3206    5623    6592   10121   13632 
   Size:       1598    1427     181    2417     969    3529    3511

  Fragments arranged by size: 

                3529    3511    2417    1598    1427     969     181
 
NheI G'CTAG_C

Cuts at:      0   10006   13632 
   Size:      10006    3626
 
NsiI A_TGCA'T

Cuts at:      0    3226   13632 
   Size:       3226   10406
 
NspI r_CATG'y

Cuts at:      0     739     783     941    5435    5616    6294    7746    9838 
   Size:        739      44     158    4494     181     678    1452    2092

Cuts at:   9838   11289   12545   12759   12953   13632 
   Size:       1451    1256     214     194     679

  Fragments arranged by size: 

                4494    2092    1452    1451    1256     739     679     678
                 214     194     181     158      44
 
PasI CC'CwG_GG

Cuts at:      0    1949    2464    6012    6949    9234    9440   13632 
   Size:       1949     515    3548     937    2285     206    4192

  Fragments arranged by size: 

                4192    3548    2285    1949     937     515     206
 
PciI A'CATG_T

Cuts at:      0     937    6290   11285   13632 
   Size:        937    5353    4995    2347

  Fragments arranged by size: 

                5353    4995    2347     937
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     863    1809    1975    2502    2732    2855    4517    4602 
   Size:        863     946     166     527     230     123    1662      85

Cuts at:   4602    4986    5810    6933    7881    8135    8249   11897   12047 
   Size:        384     824    1123     948     254     114    3648     150

Cuts at:  12047   13560   13632 
   Size:       1513      72

  Fragments arranged by size: 

                3648    1662    1513    1123     948     946     863     824
                 527     384     254     230     166     150     123     114
                  85      72
 
PfoI T'CCnGG_A

Cuts at:      0     284    1640    2600    2854    3758    5281    5755    6817 
   Size:        284    1356     960     254     904    1523     474    1062

Cuts at:   6817    7532   13632 
   Size:        715    6100

  Fragments arranged by size: 

                6100    1523    1356    1062     960     904     715     474
                 284     254
 
PmlI CAC'GTG

Cuts at:      0     843    7344    8355   12764   13632 
   Size:        843    6501    1011    4409     868

  Fragments arranged by size: 

                6501    4409    1011     868     843
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0   10720   10752   13632 
   Size:      10720      32    2880

  Fragments arranged by size: 

               10720    2880      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1638    2007    2215    3756    4124    5759    6684    7470 
   Size:       1638     369     208    1541     368    1635     925     786

Cuts at:   7470    7844    8871    9282   11604   13620   13632 
   Size:        374    1027     411    2322    2016      12

  Fragments arranged by size: 

                2322    2016    1638    1635    1541    1027     925     786
                 411     374     369     368     208      12
 
PshAI GACnn'nnGTC

Cuts at:      0    3304    4954    7475   13129   13632 
   Size:       3304    1650    2521    5654     503

  Fragments arranged by size: 

                5654    3304    2521    1650     503
 
PspOMI G'GGCC_C

Cuts at:      0     313     388     647    1864    3643    4638    5226   12789 
   Size:        313      75     259    1217    1779     995     588    7563

Cuts at:  12789   13213   13599   13632 
   Size:        424     386      33

  Fragments arranged by size: 

                7563    1779    1217     995     588     424     386     313
                 259      75      33
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2524    2556   13632 
   Size:       2524      32   11076

  Fragments arranged by size: 

               11076    2524      32
 
PstI C_TGCA'G

Cuts at:      0     103     481     545    2443    3034    3436    3577    4577 
   Size:        103     378      64    1898     591     402     141    1000

Cuts at:   4577    4965    5551    7918    7933    8857    8977    9904   11329 
   Size:        388     586    2367      15     924     120     927    1425

Cuts at:  11329   11542   12608   13144   13632 
   Size:        213    1066     536     488

  Fragments arranged by size: 

                2367    1898    1425    1066    1000     927     924     591
                 586     536     488     402     388     378     213     141
                 120     103      64      15
 
PvuII CAG'CTG

Cuts at:      0    5355    5523    6155    6504    7427    8009    8428    9059 
   Size:       5355     168     632     349     923     582     419     631

Cuts at:   9059   11351   11543   12626   13118   13632 
   Size:       2292     192    1083     492     514

  Fragments arranged by size: 

                5355    2292    1083     923     632     631     582     514
                 492     419     349     192     168
 
RsrII CG'GwC_CG

Cuts at:      0    2691    4676   13632 
   Size:       2691    1985    8956

  Fragments arranged by size: 

                8956    2691    1985
 
SacI G_AGCT'C

Cuts at:      0     332    3542    8617   13632 
   Size:        332    3210    5075    5015

  Fragments arranged by size: 

                5075    5015    3210     332
 
SacII CC_GC'GG

Cuts at:      0    3969    9042   13632 
   Size:       3969    5073    4590

  Fragments arranged by size: 

                5073    4590    3969
 
SalI G'TCGA_C

Cuts at:      0    1084   13632 
   Size:       1084   12548
 
SanDI GG'GwC_CC

Cuts at:      0    4124   13620   13632 
   Size:       4124    9496      12

  Fragments arranged by size: 

                9496    4124      12
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3277    5215    7972   13632 
   Size:       3277    1938    2757    5660

  Fragments arranged by size: 

                5660    3277    2757    1938
 
SbfI CC_TGCA'GG

Cuts at:      0    3436    4577   13632 
   Size:       3436    1141    9055

  Fragments arranged by size: 

                9055    3436    1141
 
SexAI A'CCwGG_T

Cuts at:      0    1124    1349    2495   11600   13632 
   Size:       1124     225    1146    9105    2032

  Fragments arranged by size: 

                9105    2032    1146    1124     225
 
SfcI C'TryA_G

Cuts at:      0      99     477     541    2439    3030    3126    3432    3573 
   Size:         99     378      64    1898     591      96     306     141

Cuts at:   3573    3864    4049    4573    4961    5547    5628    7914    7929 
   Size:        291     185     524     388     586      81    2286      15

Cuts at:   7929    8043    8853    8973    9900   11325   11538   12604   13140 
   Size:        114     810     120     927    1425     213    1066     536

Cuts at:  13140   13632 
   Size:        492

  Fragments arranged by size: 

                2286    1898    1425    1066     927     810     591     586
                 536     524     492     388     378     306     291     213
                 185     141     120     114      99      96      81      64
                  15
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    5225    5234   13632 
   Size:       5225       9    8398

  Fragments arranged by size: 

                8398    5225       9
 
SfoI GGC'GCC

Cuts at:      0    2134    4267    8025   11140   13632 
   Size:       2134    2133    3758    3115    2492

  Fragments arranged by size: 

                3758    3115    2492    2134    2133
 
SgrAI Cr'CCGG_yG

Cuts at:      0   11822   13632 
   Size:      11822    1810
 
SmaI CCC'GGG

Cuts at:      0    1363    4189    6031    9927   10405   13632 
   Size:       1363    2826    1842    3896     478    3227

  Fragments arranged by size: 

                3896    3227    2826    1842    1363     478
 
SmlI C'TyrA_G

Cuts at:      0     766    6625    8875    9154   13632 
   Size:        766    5859    2250     279    4478

  Fragments arranged by size: 

                5859    4478    2250     766     279
 
SpeI A'CTAG_T

Cuts at:      0    3997   13632 
   Size:       3997    9635
 
SphI G_CATG'C

Cuts at:      0     783   12759   13632 
   Size:        783   11976     873

  Fragments arranged by size: 

               11976     873     783
 
StuI AGG'CCT

Cuts at:      0    5653    6860    9299   10936   12146   12263   13457   13632 
   Size:       5653    1207    2439    1637    1210     117    1194     175

  Fragments arranged by size: 

                5653    2439    1637    1210    1207    1194     175     117
            
 
StyI C'CwwG_G

Cuts at:      0     333     695     974     998    1634    2012    2250    4165 
   Size:        333     362     279      24     636     378     238    1915

Cuts at:   4165    4601    5112    7391    8997    9182    9824   10580   11522 
   Size:        436     511    2279    1606     185     642     756     942

Cuts at:  11522   12365   13344   13542   13625   13632 
   Size:        843     979     198      83       7

  Fragments arranged by size: 

                2279    1915    1606     979     942     843     756     642
                 636     511     436     378     362     333     279     238
                 198     185      83      24       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     720    1967    2133    2708    3067    3357    3955    4068 
   Size:        720    1247     166     575     359     290     598     113

Cuts at:   4068    4178    4573    5170    5802    7013    7299    8001    8019 
   Size:        110     395     597     632    1211     286     702      18

Cuts at:   8019    8365    9010    9247    9321    9325    9754   10483   11230 
   Size:        346     645     237      74       4     429     729     747

Cuts at:  11230   11863   11889   11938   12817   13632 
   Size:        633      26      49     879     815

  Fragments arranged by size: 

                1247    1211     879     815     747     729     720     702
                 645     633     632     598     597     575     429     395
                 359     346     290     286     237     166     113     110
                  74      49      26      18       4
 
TatI w'GTAC_w

Cuts at:      0    1847    1985    4886    5681    6197    8438    9347    9476 
   Size:       1847     138    2901     795     516    2241     909     129

Cuts at:   9476   11609   11915   12044   12176   13632 
   Size:       2133     306     129     132    1456

  Fragments arranged by size: 

                2901    2241    2133    1847    1456     909     795     516
                 306     138     132     129     129
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1443    1475    2029    2061    2274    2306    5145    5177 
   Size:       1443      32     554      32     213      32    2839      32

Cuts at:   5177    5380    5412    5845    5877   11227   11259   13632 
   Size:        203      32     433      32    5350      32    2373

  Fragments arranged by size: 

                5350    2839    2373    1443     554     433     213     203
                  32      32      32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1126    2515    4680    4846    4999    7636    9592    9727 
   Size:       1126    1389    2165     166     153    2637    1956     135

Cuts at:   9727   11119   12406   13632 
   Size:       1392    1287    1226

  Fragments arranged by size: 

                2637    2165    1956    1392    1389    1287    1226    1126
                 166     153     135
 
XbaI T'CTAG_A

Cuts at:      0    6018   13632 
   Size:       6018    7614
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1762    1956    1976    5134    5811    6282    7341    8785 
   Size:       1762     194      20    3158     677     471    1059    1444

Cuts at:   8785    9447    9963   10477   11728   11898   13144   13530   13632 
   Size:        662     516     514    1251     170    1246     386     102

  Fragments arranged by size: 

                3158    1762    1444    1251    1246    1059     677     662
                 516     514     471     386     194     170     102      20
            
 
XmaI C'CCGG_G

Cuts at:      0    1361    4187    6029    9925   10403   13632 
   Size:       1361    2826    1842    3896     478    3229

  Fragments arranged by size: 

                3896    3229    2826    1842    1361     478
 
XmnI GAAnn'nnTTC

Cuts at:      0    3721    7705    8098    9086   13632 
   Size:       3721    3984     393     988    4546

  Fragments arranged by size: 

                4546    3984    3721     988     393
 
ZraI GAC'GTC

Cuts at:      0   12288   13632 
   Size:      12288    1344
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NsiI
     NspI     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI   PspOMI     PsrI     PstI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI
    SgrAI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AscI     AseI    AsiSI    AvrII    BsiWI   BssHII    BstBI
  BstZ17I     DraI    EcoRV     HpaI     MfeI     MluI     NotI     NruI
     PacI     PmeI     PsiI    PspXI     PvuI     ScaI     SgfI    SnaBI
     SrfI     SspI     SwaI     XhoI
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