Restriction map for
pF1KE2399
(Circular) (Six-base) MAPSORT of: pF1KE2399.seq Check: 6681 from: 1 to: 9968
pF1KE2399 9968 bp
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2180 4995 2180
Size: 2815 7153
AccI GT'mk_AC
Cuts at: 1723 4388 5854 7006 1723
Size: 2665 1466 1152 4685
Fragments arranged by size:
4685 2665 1466 1152
Acc65I G'GTAC_C
Cuts at: 1355 1394 6984 1355
Size: 39 5590 4339
Fragments arranged by size:
5590 4339 39
AclI AA'CG_TT
Cuts at: 9808 9808
Size: 9968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 427 1987 5483 6377 6419 6513 7793 8225
Size: 228 1560 3496 894 42 94 1280 432
Cuts at: 8225 8972 9240 199
Size: 747 268 927
Fragments arranged by size:
3496 1560 1280 927 894 747 432 268
228 94 42
AfeI AGC'GCT
Cuts at: 656 916 1894 6046 656
Size: 260 978 4152 4578
Fragments arranged by size:
4578 4152 978 260
AflIII A'CryG_T
Cuts at: 5846 7320 8424 9401 5846
Size: 1474 1104 977 6413
Fragments arranged by size:
6413 1474 1104 977
AgeI A'CCGG_T
Cuts at: 8356 8356
Size: 9968
AhdI GACnn_n'nnGTC
Cuts at: 5850 5850
Size: 9968
AleI CACnn'nnGTG
Cuts at: 6559 6559
Size: 9968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2293 2834 4376 6367 6577 2293
Size: 541 1542 1991 210 5684
Fragments arranged by size:
5684 1991 1542 541 210
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2874 2906 2874
Size: 32 9936
AlwNI CAG_nnn'CTG
Cuts at: 312 3469 4969 6456 7273 8840 312
Size: 3157 1500 1487 817 1567 1440
Fragments arranged by size:
3157 1567 1500 1487 1440 817
ApaI G_GGCC'C
Cuts at: 289 389 433 701 1067 1093 1167 3539 3862
Size: 100 44 268 366 26 74 2372 323
Cuts at: 3862 4020 4669 5798 6430 289
Size: 158 649 1129 632 3827
Fragments arranged by size:
3827 2372 1129 649 632 366 323 268
158 100 74 44 26
ApaLI G'TGCA_C
Cuts at: 8738 8738
Size: 9968
ApoI r'AATT_y
Cuts at: 3714 6972 9321 9577 3714
Size: 3258 2349 256 4105
Fragments arranged by size:
4105 3258 2349 256
AseI AT'TA_AT
Cuts at: 20 2909 20
Size: 2889 7079
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9968
AvaI C'yCGr_G
Cuts at: 807 1022 1042 1155 1198 4053 6309 6337 6988
Size: 215 20 113 43 2855 2256 28 651
Cuts at: 6988 807
Size: 3787
Fragments arranged by size:
3787 2855 2256 651 215 113 43 28
20
AvrII C'CTAG_G
Cuts at: 433 433
Size: 9968
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1339 1372 1588 1621 1339
Size: 33 216 33 9686
Fragments arranged by size:
9686 216 33 33
BamHI G'GATC_C
Cuts at: 3665 3798 5343 6993 3665
Size: 133 1545 1650 6640
Fragments arranged by size:
6640 1650 1545 133
BanI G'GyrC_C
Cuts at: 507 1078 1355 1394 2614 4147 4513 4591 6186
Size: 571 277 39 1220 1533 366 78 1595
Cuts at: 6186 6984 7398 7646 7681 507
Size: 798 414 248 35 2794
Fragments arranged by size:
2794 1595 1533 1220 798 571 414 366
277 248 78 39 35
BanII G_rGCy'C
Cuts at: 289 389 433 580 689 701 805 829 1067
Size: 100 44 147 109 12 104 24 238
Cuts at: 1067 1093 1167 1198 2076 2467 3539 3862 4020
Size: 26 74 31 878 391 1072 323 158
Cuts at: 4020 4654 4669 5252 5798 6430 6982 8012 289
Size: 634 15 583 546 632 552 1030 2245
Fragments arranged by size:
2245 1072 1030 878 634 632 583 552
546 391 323 238 158 147 109 104
100 74 44 31 26 24 15 12
BbeI G_GCGC'C
Cuts at: 511 1082 7402 7650 511
Size: 571 6320 248 2829
Fragments arranged by size:
6320 2829 571 248
BbsI GAAGACnn'nnnn_
Cuts at: 603 887 3446 603
Size: 284 2559 7125
Fragments arranged by size:
7125 2559 284
BbvCI CC'TCA_GC
Cuts at: 1241 1283 1541 1802 1847 1947 4307 4376 4574
Size: 42 258 261 45 100 2360 69 198
Cuts at: 4574 6472 1241
Size: 1898 4737
Fragments arranged by size:
4737 2360 1898 261 258 198 100 69
45 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1389 1423 4017 4051 1389
Size: 34 2594 34 7306
Fragments arranged by size:
7306 2594 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1853 4872 7866 8627 9968 1853
Size: 3019 2994 761 1341 1853
Fragments arranged by size:
3019 2994 1853 1341 761
BclI T'GATC_A
Cuts at: 2927 5522 7488 2927
Size: 2595 1966 5407
Fragments arranged by size:
5407 2595 1966
BfrBI ATG'CAT
Cuts at: 2319 2631 2811 2319
Size: 312 180 9476
Fragments arranged by size:
9476 312 180
BglI GCCn_nnn'nGGC
Cuts at: 601 2286 3866 5712 6628 601
Size: 1685 1580 1846 916 3941
Fragments arranged by size:
3941 1846 1685 1580 916
BglII A'GATC_T
Cuts at: 5285 7483 9520 5285
Size: 2198 2037 5733
Fragments arranged by size:
5733 2198 2037
BlpI GC'TnA_GC
Cuts at: 799 908 2412 4583 7080 799
Size: 109 1504 2171 2497 3687
Fragments arranged by size:
3687 2497 2171 1504 109
Bme1580I G_kGCm'C
Cuts at: 289 389 433 701 1067 1093 1167 3358 3539
Size: 100 44 268 366 26 74 2191 181
Cuts at: 3539 3862 4020 4669 4793 5798 6430 7593 7686
Size: 323 158 649 124 1005 632 1163 93
Cuts at: 7686 8742 289
Size: 1056 1515
Fragments arranged by size:
2191 1515 1163 1056 1005 649 632 366
323 268 181 158 124 100 93 74
44 26
BmgBI CAC'GTC
Cuts at: 1007 1007
Size: 9968
BmrI ACTGGGnnnn_n'
Cuts at: 1848 2288 2382 2573 3543 3740 4022 4301 4957
Size: 440 94 191 970 197 282 279 656
Cuts at: 4957 6387 7180 7261 7596 9740 1848
Size: 1430 793 81 335 2144 2076
Fragments arranged by size:
2144 2076 1430 970 793 656 440 335
282 279 197 191 94 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 6867 8335 3839
Size: 3028 1468 5472
Fragments arranged by size:
5472 3028 1468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 855 1624 1950 2001 2105 2615 2654 3020
Size: 152 769 326 51 104 510 39 366
Cuts at: 3020 3584 3809 5798 6559 703
Size: 564 225 1989 761 4112
Fragments arranged by size:
4112 1989 769 761 564 510 366 326
225 152 104 51 39
Bpu10I CC'TnA_GC
Cuts at: 1241 1283 1541 1802 1847 1947 1996 2238 4307
Size: 42 258 261 45 100 49 242 2069
Cuts at: 4307 4376 4574 6472 6632 1241
Size: 69 198 1898 160 4577
Fragments arranged by size:
4577 2069 1898 261 258 242 198 160
100 69 49 45 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1904 5119 7144 8023 8515 8813 1904
Size: 3215 2025 879 492 298 3059
Fragments arranged by size:
3215 3059 2025 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1272 1940 4484 5149 5992 9672 1272
Size: 668 2544 665 843 3680 1568
Fragments arranged by size:
3680 2544 1568 843 668 665
BsaAI yAC'GTr
Cuts at: 7321 7321
Size: 9968
BsaBI GATnn'nnATC
Cuts at: 6 3522 5284 7482 9519 6
Size: 3516 1762 2198 2037 455
Fragments arranged by size:
3516 2198 2037 1762 455
BsaHI Gr'CG_yC
Cuts at: 508 1079 7399 7647 8349 9845 508
Size: 571 6320 248 702 1496 631
Fragments arranged by size:
6320 1496 702 631 571 248
BsaWI w'CCGG_w
Cuts at: 2437 2780 5954 7157 7678 8356 8630 8777 9188
Size: 343 3174 1203 521 678 274 147 411
Cuts at: 9188 2437
Size: 3217
Fragments arranged by size:
3217 3174 1203 678 521 411 343 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1622 1652 2481 2511 2652 2667 2682 2697 2874
Size: 30 829 30 141 15 15 15 177
Cuts at: 2874 2904 6238 6268 6532 6562 1622
Size: 30 3334 30 264 30 5028
Fragments arranged by size:
5028 3334 829 264 177 141 30 30
30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 503 858 876 2144 3217 3593 5088 5479
Size: 283 355 18 1268 1073 376 1495 391
Cuts at: 5479 5527 6223 6742 220
Size: 48 696 519 3446
Fragments arranged by size:
3446 1495 1268 1073 696 519 391 376
355 283 48 18
BseYI C'CCAG_C
Cuts at: 312 543 701 968 1012 1483 1565 1664 1676
Size: 231 158 267 44 471 82 99 12
Cuts at: 1676 1715 1829 1868 1886 2402 3147 3485 4171
Size: 39 114 39 18 516 745 338 686
Cuts at: 4171 4326 4331 4337 4364 4523 5940 7393 8151
Size: 155 5 6 27 159 1417 1453 758
Cuts at: 8151 8728 9224 312
Size: 577 496 1056
Fragments arranged by size:
1453 1417 1056 758 745 686 577 516
496 471 338 267 231 159 158 155
114 99 82 44 39 39 27 18
12 6 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2603 4487 4690 4912 5017 5617 5629 6142 6688
Size: 1884 203 222 105 600 12 513 546
Cuts at: 6688 2603
Size: 5883
Fragments arranged by size:
5883 1884 600 546 513 222 203 105
12
BsiEI CG_ry'CG
Cuts at: 104 242 530 573 605 953 1151 7510 7556
Size: 138 288 43 32 348 198 6359 46
Cuts at: 7556 8764 104
Size: 1208 1308
Fragments arranged by size:
6359 1308 1208 348 288 198 138 46
43 32
BsiHKAI G_wGCw'C
Cuts at: 805 2076 2095 4012 4648 5161 5252 6982 7760
Size: 1271 19 1917 636 513 91 1730 778
Cuts at: 7760 7950 8742 805
Size: 190 792 2031
Fragments arranged by size:
2031 1917 1730 1271 792 778 636 513
190 91 19
BsmI GAATG_Cn'
Cuts at: 3424 3424
Size: 9968
Bsp1286I G_dGCh'C
Cuts at: 289 389 433 580 689 701 805 829 1067
Size: 100 44 147 109 12 104 24 238
Cuts at: 1067 1093 1167 1198 2076 2095 2467 3358 3539
Size: 26 74 31 878 19 372 891 181
Cuts at: 3539 3862 4012 4020 4648 4654 4669 4793 5161
Size: 323 150 8 628 6 15 124 368
Cuts at: 5161 5252 5798 6430 6982 7593 7686 7760 7950
Size: 91 546 632 552 611 93 74 190
Cuts at: 7950 8012 8742 289
Size: 62 730 1515
Fragments arranged by size:
1515 891 878 730 632 628 611 552
546 372 368 323 238 190 181 150
147 124 109 104 100 93 91 74
74 62 44 31 26 24 19 15
12 8 6
BspEI T'CCGG_A
Cuts at: 2437 9188 2437
Size: 6751 3217
BspHI T'CATG_A
Cuts at: 5210 9144 5210
Size: 3934 6034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 108 2180 4945 4977 4995 7018 7232 7534 7915
Size: 2072 2765 32 18 2023 214 302 381
Cuts at: 7915 108
Size: 2161
Fragments arranged by size:
2765 2161 2072 2023 381 302 214 32
18
BsrBI CCG'CTC
Cuts at: 50 665 1227 8260 8314 9798 9963 50
Size: 615 562 7033 54 1484 165 55
Fragments arranged by size:
7033 1484 615 562 165 55 54
BsrDI GCAATG_nn'
Cuts at: 351 2342 3631 4410 6102 6659 7880 351
Size: 1991 1289 779 1692 557 1221 2439
Fragments arranged by size:
2439 1991 1692 1289 1221 779 557
BsrFI r'CCGG_y
Cuts at: 370 847 952 1081 2702 3898 7966 8147 8356
Size: 477 105 129 1621 1196 4068 181 209
Cuts at: 8356 370
Size: 1982
Fragments arranged by size:
4068 1982 1621 1196 477 209 181 129
105
BsrGI T'GTAC_A
Cuts at: 4271 4271
Size: 9968
BssHII G'CGCG_C
Cuts at: 6815 6815
Size: 9968
BssSI C'ACGA_G
Cuts at: 4737 6653 8239 8597 4737
Size: 1916 1586 358 6108
Fragments arranged by size:
6108 1916 1586 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4320 4533 6655 7345 4320
Size: 213 2122 690 6943
Fragments arranged by size:
6943 2122 690 213
BstBI TT'CG_AA
Cuts at: 6072 8329 6072
Size: 2257 7711
BstEII G'GTnAC_C
Cuts at: 3877 5317 3877
Size: 1440 8528
BstXI CCAn_nnnn'nTGG
Cuts at: 144 625 1257 144
Size: 481 632 8855
Fragments arranged by size:
8855 632 481
BstYI r'GATC_y
Cuts at: 1 3494 3523 3623 3665 3798 5285 5343 5966
Size: 3493 29 100 42 133 1487 58 623
Cuts at: 5966 6993 7460 7483 7818 8064 9076 9162 9174
Size: 1027 467 23 335 246 1012 86 12
Cuts at: 9174 9520 1
Size: 346 449
Fragments arranged by size:
3493 1487 1027 1012 623 467 449 346
335 246 133 100 86 58 42 29
23 12
BstZ17I GTA'TAC
Cuts at: 4389 4389
Size: 9968
Bsu36I CC'TnA_GG
Cuts at: 2738 2936 3849 3995 4856 2738
Size: 198 913 146 861 7850
Fragments arranged by size:
7850 913 861 198 146
BtgI C'CryG_G
Cuts at: 107 293 524 599 720 926 947 1430 2057
Size: 186 231 75 121 206 21 483 627
Cuts at: 2057 2483 2609 2693 2954 2966 3212 3566 4749
Size: 426 126 84 261 12 246 354 1183
Cuts at: 4749 5381 107
Size: 632 4694
Fragments arranged by size:
4694 1183 632 627 483 426 354 261
246 231 206 186 126 121 84 75
21 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 509 3937 7918 8099 8262 509
Size: 3428 3981 181 163 2215
Fragments arranged by size:
3981 3428 2215 181 163
BtsI GCAGTG_nn'
Cuts at: 1117 2267 3372 4418 6671 7211 1117
Size: 1150 1105 1046 2253 540 3874
Fragments arranged by size:
3874 2253 1150 1105 1046 540
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 333 368 6770 6805 333
Size: 35 6402 35 3496
Fragments arranged by size:
6402 3496 35 35
DraI TTT'AAA
Cuts at: 5300 6967 5300
Size: 1667 8301
DraIII CAC_nnn'GTG
Cuts at: 2296 2527 4598 2296
Size: 231 2071 7666
Fragments arranged by size:
7666 2071 231
DrdI GACnn_nn'nnGTC
Cuts at: 3549 7674 8532 3549
Size: 4125 858 4985
Fragments arranged by size:
4985 4125 858
EaeI y'GGCC_r
Cuts at: 110 239 527 570 602 950 1148 2287 2298
Size: 129 288 43 32 348 198 1139 11
Cuts at: 2298 2723 2969 4219 4485 4885 6515 6620 6830
Size: 425 246 1250 266 400 1630 105 210
Cuts at: 6830 6954 7553 7727 8118 8145 9490 110
Size: 124 599 174 391 27 1345 588
Fragments arranged by size:
1630 1345 1250 1139 599 588 425 400
391 348 288 266 246 210 198 174
129 124 105 43 32 27 11
EagI C'GGCC_G
Cuts at: 239 527 570 602 950 1148 7553 239
Size: 288 43 32 348 198 6405 2654
Fragments arranged by size:
6405 2654 348 288 198 43 32
EarI CTCTTCn'nnn_
Cuts at: 5423 5435 7991 8201 5423
Size: 12 2556 210 7190
Fragments arranged by size:
7190 2556 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 380 644 870 1111 5655 8176 8486 8632 9778
Size: 264 226 241 4544 2521 310 146 1146
Cuts at: 9778 380
Size: 570
Fragments arranged by size:
4544 2521 1146 570 310 264 241 226
146
EcoICRI GAG'CTC
Cuts at: 803 2074 5250 6980 803
Size: 1271 3176 1730 3791
Fragments arranged by size:
3791 3176 1730 1271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 427 703 855 1624 1950 1987 2001 2105
Size: 228 276 152 769 326 37 14 104
Cuts at: 2105 2615 2654 3020 3584 3809 5483 5798 6377
Size: 510 39 366 564 225 1674 315 579
Cuts at: 6377 6419 6513 6559 7793 8225 8972 9240 199
Size: 42 94 46 1234 432 747 268 927
Fragments arranged by size:
1674 1234 927 769 747 579 564 510
432 366 326 315 276 268 228 225
152 104 94 46 42 39 37 14
EcoNI CCTnn'n_nnAGG
Cuts at: 1331 2438 3908 3986 6631 1331
Size: 1107 1470 78 2645 4668
Fragments arranged by size:
4668 2645 1470 1107 78
EcoO109I rG'GnC_Cy
Cuts at: 285 286 430 678 697 698 1064 1089 1163
Size: 1 144 248 19 1 366 25 74
Cuts at: 1163 1324 1376 1480 2188 2941 3535 3536 3858
Size: 161 52 104 708 753 594 1 322
Cuts at: 3858 3859 3955 3991 4016 4017 4665 4666 4805
Size: 1 96 36 25 1 648 1 139
Cuts at: 4805 4957 5416 5794 5795 6408 6427 7107 285
Size: 152 459 378 1 613 19 680 3146
Fragments arranged by size:
3146 753 708 680 648 613 594 459
378 366 322 248 161 152 144 139
104 96 74 52 36 25 25 19
19 1 1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 3714 6972 3714
Size: 3258 6710
EcoRV GAT'ATC
Cuts at: 5022 5022
Size: 9968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5425 7987 5425
Size: 2562 7406
FspI TGC'GCA
Cuts at: 7749 7749
Size: 9968
HaeII r_GCGC'y
Cuts at: 511 658 770 878 918 1082 1896 4443 6048
Size: 147 112 108 40 164 814 2547 1605
Cuts at: 6048 7402 7650 8672 9651 511
Size: 1354 248 1022 979 828
Fragments arranged by size:
2547 1605 1354 1022 979 828 814 248
164 147 112 108 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1090 1122 1266 1298 2268 2300 2481 2513 2667
Size: 32 144 32 970 32 181 32 154
Cuts at: 2667 2699 2919 2951 3486 3518 4050 4082 4481
Size: 32 220 32 535 32 532 32 399
Cuts at: 4481 4483 4513 4515 5836 5868 7810 7840 7842
Size: 2 30 2 1321 32 1942 30 2
Cuts at: 7842 7872 1090
Size: 30 3186
Fragments arranged by size:
3186 1942 1321 970 535 532 399 220
181 154 144 32 32 32 32 32
32 32 32 32 30 30 30 2
2 2
HincII GTy'rAC
Cuts at: 1498 1724 3032 3309 3554 7007 1498
Size: 226 1308 277 245 3453 4459
Fragments arranged by size:
4459 3453 1308 277 245 226
HindIII A'AGCT_T
Cuts at: 5539 5596 5539
Size: 57 9911
HpaI GTT'AAC
Cuts at: 3032 3032
Size: 9968
KasI G'GCGC_C
Cuts at: 507 1078 7398 7646 507
Size: 571 6320 248 2829
Fragments arranged by size:
6320 2829 571 248
KpnI G_GTAC'C
Cuts at: 1359 1398 6988 1359
Size: 39 5590 4339
Fragments arranged by size:
5590 4339 39
MluI A'CGCG_T
Cuts at: 9401 9401
Size: 9968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2285 4067 5292 6299 6863 8639 8823 2285
Size: 1782 1225 1007 564 1776 184 3430
Fragments arranged by size:
3430 1782 1776 1225 1007 564 184
MscI TGG'CCA
Cuts at: 2300 2725 2971 6517 6622 6832 6956 7729 2300
Size: 425 246 3546 105 210 124 773 4539
Fragments arranged by size:
4539 3546 773 425 246 210 124 105
MslI CAynn'nnrTG
Cuts at: 2458 3046 3217 6559 6664 7957 8084 2458
Size: 588 171 3342 105 1293 127 4342
Fragments arranged by size:
4342 3342 1293 588 171 127 105
MspA1I CmG'CkG
Cuts at: 201 238 312 377 526 601 949 1138 1208
Size: 37 74 65 149 75 348 189 70
Cuts at: 1208 1392 1487 3466 4429 4573 6397 7079 7393
Size: 184 95 1979 963 144 1824 682 314
Cuts at: 7393 7753 8766 9011 201
Size: 360 1013 245 1158
Fragments arranged by size:
1979 1824 1158 1013 963 682 360 348
314 245 189 184 149 144 95 75
74 70 65 37
NaeI GCC'GGC
Cuts at: 372 849 954 1083 3900 8149 372
Size: 477 105 129 2817 4249 2191
Fragments arranged by size:
4249 2817 2191 477 129 105
NarI GG'CG_CC
Cuts at: 508 1079 7399 7647 508
Size: 571 6320 248 2829
Fragments arranged by size:
6320 2829 571 248
NcoI C'CATG_G
Cuts at: 107 926 1430 2057 2483 2609 2693 2954 2966
Size: 819 504 627 426 126 84 261 12
Cuts at: 2966 3212 3566 4749 5381 107
Size: 246 354 1183 632 4694
Fragments arranged by size:
4694 1183 819 632 627 504 426 354
261 246 126 84 12
NdeI CA'TA_TG
Cuts at: 1290 4236 4770 1290
Size: 2946 534 6488
Fragments arranged by size:
6488 2946 534
NgoMIV G'CCGG_C
Cuts at: 370 847 952 1081 3898 8147 370
Size: 477 105 129 2817 4249 2191
Fragments arranged by size:
4249 2817 2191 477 129 105
NotI GC'GGCC_GC
Cuts at: 527 602 1148 527
Size: 75 546 9347
Fragments arranged by size:
9347 546 75
NsiI A_TGCA'T
Cuts at: 2321 2633 2813 2321
Size: 312 180 9476
Fragments arranged by size:
9476 312 180
NspI r_CATG'y
Cuts at: 2815 3780 4536 4842 5752 5850 6762 7021 7521
Size: 965 756 306 910 98 912 259 500
Cuts at: 7521 8428 2815
Size: 907 4355
Fragments arranged by size:
4355 965 912 910 907 756 500 306
259 98
PasI CC'CwG_GG
Cuts at: 1360 2502 3733 3904 4662 5236 6121 6423 1360
Size: 1142 1231 171 758 574 885 302 4905
Fragments arranged by size:
4905 1231 1142 885 758 574 302 171
PciI A'CATG_T
Cuts at: 5846 8424 5846
Size: 2578 7390
PflMI CCAn_nnn'nTGG
Cuts at: 2296 2527 2656 3733 4228 5806 2296
Size: 231 129 1077 495 1578 6458
Fragments arranged by size:
6458 1578 1077 495 231 129
PfoI T'CCnGG_A
Cuts at: 2082 3668 6275 6359 6488 2082
Size: 1586 2607 84 129 5562
Fragments arranged by size:
5562 2607 1586 129 84
PmeI GTTT'AAAC
Cuts at: 6967 6967
Size: 9968
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2874 2906 4888 4920 9107 9139 2874
Size: 32 1982 32 4187 32 3703
Fragments arranged by size:
4187 3703 1982 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1324 1376 2941 3955 4957 5416 6408 1324
Size: 52 1565 1014 1002 459 992 4884
Fragments arranged by size:
4884 1565 1014 1002 992 459 52
PshAI GACnn'nnGTC
Cuts at: 1329 3304 1329
Size: 1975 7993
PspOMI G'GGCC_C
Cuts at: 285 385 429 697 1063 1089 1163 3535 3858
Size: 100 44 268 366 26 74 2372 323
Cuts at: 3858 4016 4665 5794 6426 285
Size: 158 649 1129 632 3827
Fragments arranged by size:
3827 2372 1129 649 632 366 323 268
158 100 74 44 26
PstI C_TGCA'G
Cuts at: 329 662 2262 2985 7015 7700 329
Size: 333 1600 723 4030 685 2597
Fragments arranged by size:
4030 2597 1600 723 685 333
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9968
PvuII CAG'CTG
Cuts at: 1138 3466 4429 4573 6397 7393 7753 1138
Size: 2328 963 144 1824 996 360 3353
Fragments arranged by size:
3353 2328 1824 996 963 360 144
RsrII CG'GwC_CG
Cuts at: 8163 8163
Size: 9968
SacI G_AGCT'C
Cuts at: 805 2076 5252 6982 805
Size: 1271 3176 1730 3791
Fragments arranged by size:
3791 3176 1730 1271
SacII CC_GC'GG
Cuts at: 527 602 950 527
Size: 75 348 9545
Fragments arranged by size:
9545 348 75
SalI G'TCGA_C
Cuts at: 1722 7005 1722
Size: 5283 4685
SanDI GG'GwC_CC
Cuts at: 1376 2941 1376
Size: 1565 8403
SapI GCTCTTCn'nnn_
Cuts at: 7991 8201 7991
Size: 210 9758
SbfI CC_TGCA'GG
Cuts at: 7015 7015
Size: 9968
ScaI AGT'ACT
Cuts at: 9485 9485
Size: 9968
SfcI C'TryA_G
Cuts at: 33 325 658 1053 2258 2981 3885 7011 7696
Size: 292 333 395 1205 723 904 3126 685
Cuts at: 7696 8689 8880 33
Size: 993 191 1121
Fragments arranged by size:
3126 1205 1121 993 904 723 685 395
333 292 191
SfoI GGC'GCC
Cuts at: 509 1080 7400 7648 509
Size: 571 6320 248 2829
Fragments arranged by size:
6320 2829 571 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9968
SmaI CCC'GGG
Cuts at: 1024 1044 1200 6990 1024
Size: 20 156 5790 4002
Fragments arranged by size:
5790 4002 156 20
SmlI C'TyrA_G
Cuts at: 1919 5134 7123 8038 8530 8792 1919
Size: 3215 1989 915 492 262 3095
Fragments arranged by size:
3215 3095 1989 915 492 262
SpeI A'CTAG_T
Cuts at: 67 6784 67
Size: 6717 3251
SphI G_CATG'C
Cuts at: 4842 7021 7521 4842
Size: 2179 500 7289
Fragments arranged by size:
7289 2179 500
SspI AAT'ATT
Cuts at: 5263 5263
Size: 9968
StyI C'CwwG_G
Cuts at: 107 433 926 1430 2057 2115 2483 2609 2693
Size: 326 493 504 627 58 368 126 84
Cuts at: 2693 2954 2966 3015 3212 3566 4143 4749 5381
Size: 261 12 49 197 354 577 606 632
Cuts at: 5381 6222 6287 6827 6833 7102 7456 107
Size: 841 65 540 6 269 354 2619
Fragments arranged by size:
2619 841 632 627 606 577 540 504
493 368 354 354 326 269 261 197
126 84 65 58 49 12 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2307 2602 2947 3087 3139 3165 3865 4156 5305
Size: 295 345 140 52 26 700 291 1149
Cuts at: 5305 5849 5890 6134 6185 6593 7551 7684 8143
Size: 544 41 244 51 408 958 133 459
Cuts at: 8143 8352 2307
Size: 209 3923
Fragments arranged by size:
3923 1149 958 700 544 459 408 345
295 291 244 209 140 133 52 51
41 26
TatI w'GTAC_w
Cuts at: 740 2390 4271 6939 9483 740
Size: 1650 1881 2668 2544 1225
Fragments arranged by size:
2668 2544 1881 1650 1225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1622 1654 2652 2684 6238 6270 9485 9517 1622
Size: 32 998 32 3554 32 3215 32 2073
Fragments arranged by size:
3554 3215 2073 998 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4495 7765 4495
Size: 3270 6698
XbaI T'CTAG_A
Cuts at: 6999 6999
Size: 9968
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 622 2586 3348 3564 3870 4768 7463 622
Size: 1964 762 216 306 898 2695 3127
Fragments arranged by size:
3127 2695 1964 898 762 306 216
XmaI C'CCGG_G
Cuts at: 1022 1042 1198 6988 1022
Size: 20 156 5790 4002
Fragments arranged by size:
5790 4002 156 20
XmnI GAAnn'nnTTC
Cuts at: 5408 5408
Size: 9968
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI BmtI BsiWI BsmBI ClaI FseI
FspAI MfeI NheI NruI PacI PmlI PsiI PspXI
PsrI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI