Restriction map for pF1KE2484
##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

Acc65I      AclI        AflII       AocI        AscI        
AseI        AsnI        Asp718I     AspI        AvrII       
BfrI        BlnI        BsiWI       BsmI        BspDI       
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        Eco81I      EcoRI       
EcoRV       HindIII     HpaI        KpnI        MluI        
NdeI        NotI        NruI        NspV        PacI        
PflFI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SfuI        SgrAI       SnaBI       
SspI        SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AatII        5958  GACGTC               GACGT^C
AccIII       6992  TCCGGA               T^CCGGA
AgeI         4379  ACCGGT               A^CCGGT
Asp700I      4308  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI        3838  CACGTG               CAC^GTG
BseAI        6992  TCCGGA               T^CCGGA
Bsp1407I     1322  TGTACA               T^GTACA
BspEI        6992  TCCGGA               T^CCGGA
BsrGI        1322  TGTACA               T^GTACA
BstEII       4875  GGTCACC              G^GT[ACGT]ACC
BstPI        4875  GGTCACC              G^GT[ACGT]ACC
DraIII       1477  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      4875  GGTCACC              G^GT[ACGT]ACC
EcoT22I      2650  ATGCAT               ATGCA^T
FseI           88  GGCCGGCC             GGCCGG^CC
KspI          208  CCGCGG               CCGC^GG
MfeI         5406  CAATTG               C^AATTG
MroI         6992  TCCGGA               T^CCGGA
MunI         5406  CAATTG               C^AATTG
NheI         1270  GCTAGC               G^CTAGC
NsiI         2650  ATGCAT               ATGCA^T
PaeR7I       3745  CTCGAG               C^TCGAG
PinAI        4379  ACCGGT               A^CCGGT
PmaCI        3838  CACGTG               CAC^GTG
PmlI         3838  CACGTG               CAC^GTG
SacII         208  CCGCGG               CCGC^GG
SbfI          348  CCTGCAGG             CCTGCA^GG
SpeI         1818  ACTAGT               A^CTAGT
Sse8387I      348  CCTGCAGG             CCTGCA^GG
SspBI        1322  TGTACA               T^GTACA
XhoI         3745  CTCGAG               C^TCGAG
XmnI         4308  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI         1069  GTCGAC               GT^[AC][GT]AC
             2965  GTCGAC              
AcsI          735  GAATTT               [GA]^AATT[CT]
             2712  GAATTT              
AflIII       4589  ACATGT               A^C[GA][CT]GT
             6155  ACATGT              
AhdI         3754  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5446  GACCAGGAGTC         
ApoI          735  GAATTT               [GA]^AATT[CT]
             2712  GAATTT              
AspEI        3754  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5446  GACCAGGAGTC         
AviII         590  TGCGCA               TGC^GCA
             5141  TGCGCA              
BbvCI        5631  CCTCAGC              CCTCAGC(-5/-2)
             4128  GCTGAGG             
BclI         1157  TGATCA               T^GATCA
             4172  TGATCA              
BsaAI        2857  TACGTG               [CT]AC^GT[GA]
             3838  CACGTG              
BsaBI        1032  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2250  GATCGCCATC          
BsgI         5725  CTGCAC               GTGCAG(16/14)
             4291  GTGCAG              
BsmBI        1798  GAGACG               CGTCTC(1/5)
             4207  GAGACG              
BspLU11I     4589  ACATGT               A^CATGT
             6155  ACATGT              
BssSI        2293  CACGAG               CACGAG(-5/-1)
             3241  CACGAG              
DrdI         3835  GACCACGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4209  GACGCTGATGTC        
Eam1105I     3754  GACTCCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5446  GACCAGGAGTC         
FbaI         1157  TGATCA               T^GATCA
             4172  TGATCA              
FspI          590  TGCGCA               TGC^GCA
             5141  TGCGCA              
MamI         1032  GATCGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2250  GATCGCCATC          
SalI         1069  GTCGAC               G^TCGAC
             2965  GTCGAC              
SexAI        1316  ACCTGGT              A^CC[AT]GGT
             1478  ACCTGGT             
SfiI         1539  GGCCCCCCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             5863  GGCCCAGGGGGCC       
StuI          353  AGGCCT               AGG^CCT
              908  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  153 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       Aor51HI     ApaI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BamHI       BanI        BanII       BbeI        BbsI        
BbvI        BcgI        BciVI       BcnI        BfaI        
BglI        BglII       BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspHI       BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RcaI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SfoI        SmaI        SmlI        SphI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com