Restriction map for
pF1KE2484
(Circular) (Six-base) MAPSORT of: pF1KE2484.seq Check: 3347 from: 1 to: 10244
pF1KE2484 10244 bp
With 182 enzymes: SgfI *
July 18, 2019 20:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1471 3633 5575 1471
Size: 2162 1942 6140
Fragments arranged by size:
6140 2162 1942
AatII G_ACGT'C
Cuts at: 6070 6070
Size: 10244
AccI GT'mk_AC
Cuts at: 1178 3074 7282 1178
Size: 1896 4208 4140
Fragments arranged by size:
4208 4140 1896
Acc65I G'GTAC_C
Cuts at: 7260 7260
Size: 10244
AclI AA'CG_TT
Cuts at: 10084 10084
Size: 10244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1933 2503 2653 2727 3484 3704 4321 5056 5287
Size: 570 150 74 757 220 617 735 231
Cuts at: 5287 5296 5968 6856 8069 8501 9248 9516 1933
Size: 9 672 888 1213 432 747 268 2661
Fragments arranged by size:
2661 1213 888 757 747 735 672 617
570 432 268 231 220 150 74 9
AfeI AGC'GCT
Cuts at: 445 877 3402 4020 4857 445
Size: 432 2525 618 837 5832
Fragments arranged by size:
5832 2525 837 618 432
AflIII A'CryG_T
Cuts at: 4697 6263 7596 8700 9677 4697
Size: 1566 1333 1104 977 5264
Fragments arranged by size:
5264 1566 1333 1104 977
AgeI A'CCGG_T
Cuts at: 4487 8632 4487
Size: 4145 6099
AhdI GACnn_n'nnGTC
Cuts at: 3867 5559 3867
Size: 1692 8552
AleI CACnn'nnGTG
Cuts at: 5432 5432
Size: 10244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 766 976 2983 3680 766
Size: 210 2007 697 7330
Fragments arranged by size:
7330 2007 697 210
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 10212
AlwNI CAG_nnn'CTG
Cuts at: 1550 1762 2865 3337 3395 4299 4533 5022 5325
Size: 212 1103 472 58 904 234 489 303
Cuts at: 5325 6311 6543 7549 9116 1550
Size: 986 232 1006 1567 2678
Fragments arranged by size:
2678 1567 1103 1006 986 904 489 472
303 234 232 212 58
ApaI G_GGCC'C
Cuts at: 1650 1743 1955 1991 2260 3298 3741 5800 6296
Size: 93 212 36 269 1038 443 2059 496
Cuts at: 6296 6584 6730 1650
Size: 288 146 5164
Fragments arranged by size:
5164 2059 1038 496 443 288 269 212
146 93 36
ApaLI G'TGCA_C
Cuts at: 1591 1765 1885 3452 4929 9014 1591
Size: 174 120 1567 1477 4085 2821
Fragments arranged by size:
4085 2821 1567 1477 174 120
ApoI r'AATT_y
Cuts at: 843 2820 7248 9597 9853 843
Size: 1977 4428 2349 256 1234
Fragments arranged by size:
4428 2349 1977 1234 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10244
AvaI C'yCGr_G
Cuts at: 228 1472 1519 1652 1984 2120 2410 3734 3853
Size: 1244 47 133 332 136 290 1324 119
Cuts at: 3853 5258 6599 7054 7264 228
Size: 1405 1341 455 210 3208
Fragments arranged by size:
3208 1405 1341 1324 1244 455 332 290
210 136 133 119 47
BamHI G'GATC_C
Cuts at: 452 3824 7131 7269 452
Size: 3372 3307 138 3427
Fragments arranged by size:
3427 3372 3307 138
BanI G'GyrC_C
Cuts at: 133 687 1105 1815 2032 3645 3725 4216 6442
Size: 554 418 710 217 1613 80 491 2226
Cuts at: 6442 6500 7260 7674 7922 7957 133
Size: 58 760 414 248 35 2420
Fragments arranged by size:
2420 2226 1613 760 710 554 491 418
414 248 217 80 58 35
BanII G_rGCy'C
Cuts at: 910 1255 1394 1650 1743 1955 1991 2147 2260
Size: 345 139 256 93 212 36 156 113
Cuts at: 2260 2273 3116 3298 3544 3741 3834 5399 5800
Size: 13 843 182 246 197 93 1565 401
Cuts at: 5800 5832 5932 6296 6320 6584 6730 6818 7113
Size: 32 100 364 24 264 146 88 295
Cuts at: 7113 7258 8288 910
Size: 145 1030 2866
Fragments arranged by size:
2866 1565 1030 843 401 364 345 295
264 256 246 212 197 182 156 146
145 139 113 100 93 93 88 36
32 24 13
BbeI G_GCGC'C
Cuts at: 137 1109 1819 3729 6504 7678 7926 137
Size: 972 710 1910 2775 1174 248 2455
Fragments arranged by size:
2775 2455 1910 1174 972 710 248
BbsI GAAGACnn'nnnn_
Cuts at: 762 2442 2692 4797 5740 6563 762
Size: 1680 250 2105 943 823 4443
Fragments arranged by size:
4443 2105 1680 943 823 250
BbvCI CC'TCA_GC
Cuts at: 4237 5740 4237
Size: 1503 8741
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2373 2407 3966 4000 4816 4850 2373
Size: 34 1559 34 816 34 7767
Fragments arranged by size:
7767 1559 816 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 717 1886 4434 8142 8903 10244 717
Size: 1169 2548 3708 761 1341 717
Fragments arranged by size:
3708 2548 1341 1169 761 717
BclI T'GATC_A
Cuts at: 1265 4280 7764 1265
Size: 3015 3484 3745
Fragments arranged by size:
3745 3484 3015
BfrBI ATG'CAT
Cuts at: 2760 2760
Size: 10244
BglI GCCn_nnn'nGGC
Cuts at: 1654 3569 5978 6017 6498 1654
Size: 1915 2409 39 481 5400
Fragments arranged by size:
5400 2409 1915 481 39
BglII A'GATC_T
Cuts at: 2054 5039 6665 7759 9796 2054
Size: 2985 1626 1094 2037 2502
Fragments arranged by size:
2985 2502 2037 1626 1094
BlpI GC'TnA_GC
Cuts at: 1077 1501 4848 5714 6769 7356 1077
Size: 424 3347 866 1055 587 3965
Fragments arranged by size:
3965 3347 1055 866 587 424
Bme1580I G_kGCm'C
Cuts at: 692 965 1595 1650 1743 1769 1889 1955 1991
Size: 273 630 55 93 26 120 66 36
Cuts at: 1991 2121 2260 3298 3456 3741 4933 5800 6156
Size: 130 139 1038 158 285 1192 867 356
Cuts at: 6156 6296 6445 6584 6730 6961 7869 7962 9018
Size: 140 149 139 146 231 908 93 1056
Cuts at: 9018 692
Size: 1918
Fragments arranged by size:
1918 1192 1056 1038 908 867 630 356
285 273 231 158 149 146 140 139
139 130 120 93 93 66 55 36
26
BmgBI CAC'GTC
Cuts at: 2915 2915
Size: 10244
BmrI ACTGGGnnnn_n'
Cuts at: 4111 4866 5616 5773 6726 7456 7537 7872 10016
Size: 755 750 157 953 730 81 335 2144
Cuts at: 10016 4111
Size: 4339
Fragments arranged by size:
4339 2144 953 755 750 730 335 157
81
BmtI G_CTAG'C
Cuts at: 1382 1382
Size: 10244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2292 3558 3853 3879 7127 8611 2292
Size: 1266 295 26 3248 1484 3925
Fragments arranged by size:
3925 3248 1484 1266 295 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 892 1014 1186 2035 2043 2313 2491 3414
Size: 270 122 172 849 8 270 178 923
Cuts at: 3414 3417 3619 4816 5203 5431 5689 5740 5746
Size: 3 202 1197 387 228 258 51 6
Cuts at: 5746 5896 5956 6178 6400 6716 622
Size: 150 60 222 222 316 4150
Fragments arranged by size:
4150 1197 923 849 387 316 270 270
258 228 222 222 202 178 172 150
122 60 51 8 6 3
Bpu10I CC'TnA_GC
Cuts at: 670 859 1463 1642 3796 4237 4273 5740 670
Size: 189 604 179 2154 441 36 1467 5174
Fragments arranged by size:
5174 2154 1467 604 441 189 179 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 1402 7420 8299 8791 9089 318
Size: 1084 6018 879 492 298 1473
Fragments arranged by size:
6018 1473 1084 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1300 1473 1525 4209 5399 6730 6779 9948 1300
Size: 173 52 2684 1190 1331 49 3169 1596
Fragments arranged by size:
3169 2684 1596 1331 1190 173 52 49
BsaAI yAC'GTr
Cuts at: 2967 3948 7597 2967
Size: 981 3649 5614
Fragments arranged by size:
5614 3649 981
BsaBI GATnn'nnATC
Cuts at: 6 1144 2362 7758 9795 6
Size: 1138 1218 5396 2037 455
Fragments arranged by size:
5396 2037 1218 1138 455
BsaHI Gr'CG_yC
Cuts at: 134 794 1106 1723 1816 3726 3776 6067 6501
Size: 660 312 617 93 1910 50 2291 434
Cuts at: 6501 7675 7923 8625 10121 134
Size: 1174 248 702 1496 257
Fragments arranged by size:
2291 1910 1496 1174 702 660 617 434
312 257 248 93 50
BsaWI w'CCGG_w
Cuts at: 1902 2813 3917 4487 7100 7204 7433 7954 8632
Size: 911 1104 570 2613 104 229 521 678
Cuts at: 8632 8906 9053 9464 1902
Size: 274 147 411 2682
Fragments arranged by size:
2682 2613 1104 911 678 570 521 411
274 229 147 104
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 312 342 1012 1042 1881 1911 4111 4141 6043
Size: 30 670 30 839 30 2200 30 1902
Cuts at: 6043 6073 6925 6955 312
Size: 30 852 30 3601
Fragments arranged by size:
3601 2200 1902 852 839 670 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 133 154 1228 1423 2428 3550 3565 3878
Size: 3 21 1074 195 1005 1122 15 313
Cuts at: 3878 4771 4789 4903 5686 5710 6826 6918 7119
Size: 893 18 114 783 24 1116 92 201
Cuts at: 7119 130
Size: 3255
Fragments arranged by size:
3255 1122 1116 1074 1005 893 783 313
201 195 114 92 24 21 18 15
3
BseYI C'CCAG_C
Cuts at: 440 691 738 923 1172 1509 1535 1540 2207
Size: 251 47 185 249 337 26 5 667
Cuts at: 2207 2272 3130 3291 3367 3457 5747 5838 6007
Size: 65 858 161 76 90 2290 91 169
Cuts at: 6007 6311 6359 7081 7221 7669 8427 9004 9500
Size: 304 48 722 140 448 758 577 496
Cuts at: 9500 440
Size: 1184
Fragments arranged by size:
2290 1184 858 758 722 667 577 496
448 337 304 251 249 185 169 161
140 91 90 76 65 48 47 26
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4420 5818 4420
Size: 1398 8846
BsiEI CG_ry'CG
Cuts at: 104 202 667 2154 3239 3450 7786 7832 9040
Size: 98 465 1487 1085 211 4336 46 1208
Cuts at: 9040 104
Size: 1308
Fragments arranged by size:
4336 1487 1308 1208 1085 465 211 98
46
BsiHKAI G_wGCw'C
Cuts at: 1091 1595 1769 1889 2147 2681 2972 3191 3268
Size: 504 174 120 258 534 291 219 77
Cuts at: 3268 3456 3544 3695 4172 4933 5003 5399 5882
Size: 188 88 151 477 761 70 396 483
Cuts at: 5882 6283 7258 8036 8226 9018 1091
Size: 401 975 778 190 792 2317
Fragments arranged by size:
2317 975 792 778 761 534 504 483
477 401 396 291 258 219 190 188
174 151 120 88 77 70
BsmBI CGTCTCn'nnnn_
Cuts at: 1900 4309 1900
Size: 2409 7835
Bsp1286I G_dGCh'C
Cuts at: 692 910 965 1091 1255 1394 1595 1650 1743
Size: 218 55 126 164 139 201 55 93
Cuts at: 1743 1769 1889 1955 1991 2121 2147 2260 2273
Size: 26 120 66 36 130 26 113 13
Cuts at: 2273 2681 2972 3116 3191 3268 3298 3456 3544
Size: 408 291 144 75 77 30 158 88
Cuts at: 3544 3695 3741 3834 4172 4933 5003 5399 5800
Size: 151 46 93 338 761 70 396 401
Cuts at: 5800 5832 5882 5932 6156 6283 6296 6320 6445
Size: 32 50 50 224 127 13 24 125
Cuts at: 6445 6584 6730 6818 6961 7113 7258 7869 7962
Size: 139 146 88 143 152 145 611 93
Cuts at: 7962 8036 8226 8288 9018 692
Size: 74 190 62 730 1918
Fragments arranged by size:
1918 761 730 611 408 401 396 338
291 224 218 201 190 164 158 152
151 146 145 144 143 139 139 130
127 126 125 120 113 93 93 93
88 88 77 75 74 70 66 62
55 55 50 50 46 36 32 30
26 26 24 13 13
BspEI T'CCGG_A
Cuts at: 7100 9464 7100
Size: 2364 7880
BspHI T'CATG_A
Cuts at: 1262 2354 2681 2948 4625 5237 9420 1262
Size: 1092 327 267 1677 612 4183 2086
Fragments arranged by size:
4183 2086 1677 1092 612 327 267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 626 1471 3633 3635 4139 5575 6569 7294 7508
Size: 845 2162 2 504 1436 994 725 214
Cuts at: 7508 7810 8191 626
Size: 302 381 2679
Fragments arranged by size:
2679 2162 1436 994 845 725 504 381
302 214 2
BsrBI CCG'CTC
Cuts at: 50 130 669 2790 3489 3555 3603 4272 8536
Size: 80 539 2121 699 66 48 669 4264
Cuts at: 8536 8590 10074 10239 50
Size: 54 1484 165 55
Fragments arranged by size:
4264 2121 1484 699 669 539 165 80
66 55 54 48
BsrDI GCAATG_nn'
Cuts at: 1638 2939 3711 5252 8156 1638
Size: 1301 772 1541 2904 3726
Fragments arranged by size:
3726 2904 1541 1301 772
BsrFI r'CCGG_y
Cuts at: 197 566 1697 2237 2652 4487 6875 7007 8242
Size: 369 1131 540 415 1835 2388 132 1235
Cuts at: 8242 8423 8632 197
Size: 181 209 1809
Fragments arranged by size:
2388 1835 1809 1235 1131 540 415 369
209 181 132
BsrGI T'GTAC_A
Cuts at: 1430 1430
Size: 10244
BssSI C'ACGA_G
Cuts at: 2401 3349 8515 8873 2401
Size: 948 5166 358 3772
Fragments arranged by size:
5166 3772 948 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1347 1550 2010 5325 6246 7621 1347
Size: 203 460 3315 921 1375 3970
Fragments arranged by size:
3970 3315 1375 921 460 203
BstBI TT'CG_AA
Cuts at: 8605 8605
Size: 10244
BstEII G'GTnAC_C
Cuts at: 4983 4983
Size: 10244
BstXI CCAn_nnnn'nTGG
Cuts at: 1374 2997 3990 4227 4995 6991 1374
Size: 1623 993 237 768 1996 4627
Fragments arranged by size:
4627 1996 1623 993 768 237
BstYI r'GATC_y
Cuts at: 1 452 1667 2054 2861 3824 4223 5039 6032
Size: 451 1215 387 807 963 399 816 993
Cuts at: 6032 6665 7131 7269 7736 7759 8094 8340 9352
Size: 633 466 138 467 23 335 246 1012
Cuts at: 9352 9438 9450 9796 1
Size: 86 12 346 449
Fragments arranged by size:
1215 1012 993 963 816 807 633 467
466 451 449 399 387 346 335 246
138 86 23 12
BtgI C'CryG_G
Cuts at: 107 316 494 2690 3989 4199 4988 4994 5885
Size: 209 178 2196 1299 210 789 6 891
Cuts at: 5885 6182 6826 107
Size: 297 644 3525
Fragments arranged by size:
3525 2196 1299 891 789 644 297 210
209 178 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 411 573 3565 3643 3969 8194 8375 8538 411
Size: 162 2992 78 326 4225 181 163 2117
Fragments arranged by size:
4225 2992 2117 326 181 163 162 78
BtsI GCAGTG_nn'
Cuts at: 3579 3637 3662 6281 7487 3579
Size: 58 25 2619 1206 6336
Fragments arranged by size:
6336 2619 1206 58 25
DraI TTT'AAA
Cuts at: 7243 7243
Size: 10244
DraIII CAC_nnn'GTG
Cuts at: 1590 1590
Size: 10244
DrdI GACnn_nn'nnGTC
Cuts at: 3949 4323 7950 8808 3949
Size: 374 3627 858 5385
Fragments arranged by size:
5385 3627 858 374
EaeI y'GGCC_r
Cuts at: 199 412 2343 2702 3236 3447 3672 4524 4991
Size: 213 1931 359 534 211 225 852 467
Cuts at: 4991 5147 6804 7829 8003 8394 8421 9766 199
Size: 156 1657 1025 174 391 27 1345 677
Fragments arranged by size:
1931 1657 1345 1025 852 677 534 467
391 359 225 213 211 174 156 27
EagI C'GGCC_G
Cuts at: 199 3236 3447 7829 199
Size: 3037 211 4382 2614
Fragments arranged by size:
4382 3037 2614 211
EarI CTCTTCn'nnn_
Cuts at: 2734 2770 2977 3199 3527 3542 4411 4748 4957
Size: 36 207 222 328 15 869 337 209
Cuts at: 4957 5359 5497 8267 8477 2734
Size: 402 138 2770 210 4501
Fragments arranged by size:
4501 2770 869 402 337 328 222 210
209 207 138 36 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 265 2605 3028 3892 8452 8762 8908 10054 265
Size: 2340 423 864 4560 310 146 1146 455
Fragments arranged by size:
4560 2340 1146 864 455 423 310 146
EcoICRI GAG'CTC
Cuts at: 2145 3542 5397 7256 2145
Size: 1397 1855 1859 5133
Fragments arranged by size:
5133 1859 1855 1397
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 892 1014 1186 1933 2035 2043 2313 2491
Size: 270 122 172 747 102 8 270 178
Cuts at: 2491 2503 2653 2727 3414 3417 3484 3619 3704
Size: 12 150 74 687 3 67 135 85
Cuts at: 3704 4321 4816 5056 5203 5287 5296 5431 5689
Size: 617 495 240 147 84 9 135 258
Cuts at: 5689 5740 5746 5896 5956 5968 6178 6400 6716
Size: 51 6 150 60 12 210 222 316
Cuts at: 6716 6856 8069 8501 9248 9516 622
Size: 140 1213 432 747 268 1350
Fragments arranged by size:
1350 1213 747 747 687 617 495 432
316 270 270 268 258 240 222 210
178 172 150 150 147 140 135 135
122 102 85 84 74 67 60 51
12 12 9 8 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1441 2292 2869 3283 4525 5329 6698 1441
Size: 851 577 414 1242 804 1369 4987
Fragments arranged by size:
4987 1369 1242 851 804 577 414
EcoO109I rG'GnC_Cy
Cuts at: 225 1646 1647 1691 1739 1740 1829 1952 1988
Size: 1421 1 44 48 1 89 123 36
Cuts at: 1988 2226 3295 3593 3738 4530 5322 5439 5796
Size: 238 1069 298 145 792 792 117 357
Cuts at: 5796 5797 6292 6293 6581 6726 6727 6831 6997
Size: 1 495 1 288 145 1 104 166
Cuts at: 6997 7066 7383 225
Size: 69 317 3086
Fragments arranged by size:
3086 1421 1069 792 792 495 357 317
298 288 238 166 145 145 123 117
104 89 69 48 44 36 1 1
1 1 1
EcoRI G'AATT_C
Cuts at: 7248 7248
Size: 10244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3180 3636 4527 7152 8263 3180
Size: 456 891 2625 1111 5161
Fragments arranged by size:
5161 2625 1111 891 456
FseI GG_CCGG'CC
Cuts at: 201 201
Size: 10244
FspI TGC'GCA
Cuts at: 700 5251 8025 700
Size: 4551 2774 2919
Fragments arranged by size:
4551 2919 2774
FspAI rTGC'GCAy
Cuts at: 700 700
Size: 10244
HaeII r_GCGC'y
Cuts at: 137 274 447 879 1109 1819 2659 3292 3374
Size: 137 173 432 230 710 840 633 82
Cuts at: 3374 3404 3429 3729 3999 4022 4785 4859 5302
Size: 30 25 300 270 23 763 74 443
Cuts at: 5302 6431 6504 6876 7678 7926 8948 9927 137
Size: 1129 73 372 802 248 1022 979 454
Fragments arranged by size:
1129 1022 979 840 802 763 710 633
454 443 432 372 300 270 248 230
173 137 82 74 73 30 25 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 312 344 1188 1220 3109 3141 3343 3375 3478
Size: 32 844 32 1889 32 202 32 103
Cuts at: 3478 3510 3853 3885 4797 4829 5545 5577 5896
Size: 32 343 32 912 32 716 32 319
Cuts at: 5896 5928 6039 6043 6064 6071 6075 6096 6805
Size: 32 111 4 21 7 4 21 709
Cuts at: 6805 6837 6899 6925 6931 6957 7050 7082 8086
Size: 32 62 26 6 26 93 32 1004
Cuts at: 8086 8116 8118 8148 312
Size: 30 2 30 2408
Fragments arranged by size:
2408 1889 1004 912 844 716 709 343
319 202 111 103 93 62 32 32
32 32 32 32 32 32 32 32
32 30 30 26 26 21 21 7
6 4 4 2
HincII GTy'rAC
Cuts at: 1050 1179 2373 3075 5199 5637 7283 1050
Size: 129 1194 702 2124 438 1646 4011
Fragments arranged by size:
4011 2124 1646 1194 702 438 129
KasI G'GCGC_C
Cuts at: 133 1105 1815 3725 6500 7674 7922 133
Size: 972 710 1910 2775 1174 248 2455
Fragments arranged by size:
2775 2455 1910 1174 972 710 248
KpnI G_GTAC'C
Cuts at: 7264 7264
Size: 10244
MfeI C'AATT_G
Cuts at: 5514 5514
Size: 10244
MluI A'CGCG_T
Cuts at: 9677 9677
Size: 10244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1385 4123 4916 6047 6737 8915 9099 1385
Size: 2738 793 1131 690 2178 184 2530
Fragments arranged by size:
2738 2530 2178 1131 793 690 184
MscI TGG'CCA
Cuts at: 2704 4526 4993 5149 8005 2704
Size: 1822 467 156 2856 4943
Fragments arranged by size:
4943 2856 1822 467 156
MslI CAynn'nnrTG
Cuts at: 367 1246 2560 2995 3247 3988 4192 4702 4993
Size: 879 1314 435 252 741 204 510 291
Cuts at: 4993 5242 5314 5432 8233 8360 367
Size: 249 72 118 2801 127 2251
Fragments arranged by size:
2801 2251 1314 879 741 510 435 291
252 249 204 127 118 72
MspA1I CmG'CkG
Cuts at: 318 640 801 1081 1317 1329 1400 1550 1808
Size: 322 161 280 236 12 71 150 258
Cuts at: 1808 1865 2105 2664 2670 3066 3323 3413 4214
Size: 57 240 559 6 396 257 90 801
Cuts at: 4214 5139 5842 5898 6926 6938 7355 7669 8029
Size: 925 703 56 1028 12 417 314 360
Cuts at: 8029 9042 9287 318
Size: 1013 245 1275
Fragments arranged by size:
1275 1028 1013 925 801 703 559 417
396 360 322 314 280 258 257 245
240 236 161 150 90 71 57 56
12 12 6
NaeI GCC'GGC
Cuts at: 199 1699 2239 2654 6877 7009 8425 199
Size: 1500 540 415 4223 132 1416 2018
Fragments arranged by size:
4223 2018 1500 1416 540 415 132
NarI GG'CG_CC
Cuts at: 134 1106 1816 3726 6501 7675 7923 134
Size: 972 710 1910 2775 1174 248 2455
Fragments arranged by size:
2775 2455 1910 1174 972 710 248
NcoI C'CATG_G
Cuts at: 107 2690 3989 4199 4988 4994 107
Size: 2583 1299 210 789 6 5357
Fragments arranged by size:
5357 2583 1299 789 210 6
NgoMIV G'CCGG_C
Cuts at: 197 1697 2237 2652 6875 7007 8423 197
Size: 1500 540 415 4223 132 1416 2018
Fragments arranged by size:
4223 2018 1500 1416 540 415 132
NheI G'CTAG_C
Cuts at: 1378 1378
Size: 10244
NsiI A_TGCA'T
Cuts at: 2762 2762
Size: 10244
NspI r_CATG'y
Cuts at: 372 408 699 945 2718 2760 4236 4701 5295
Size: 36 291 246 1773 42 1476 465 594
Cuts at: 5295 6135 6267 7297 7797 8704 372
Size: 840 132 1030 500 907 1912
Fragments arranged by size:
1912 1773 1476 1030 907 840 594 500
465 291 246 132 42 36
PasI CC'CwG_GG
Cuts at: 399 3081 3180 5974 6297 399
Size: 2682 99 2794 323 4346
Fragments arranged by size:
4346 2794 2682 323 99
PciI A'CATG_T
Cuts at: 4697 6263 8700 4697
Size: 1566 2437 6241
Fragments arranged by size:
6241 2437 1566
PflMI CCAn_nnn'nTGG
Cuts at: 1587 3766 4944 5022 1587
Size: 2179 1178 78 6809
Fragments arranged by size:
6809 2179 1178 78
PfoI T'CCnGG_A
Cuts at: 1670 2222 5120 5543 1670
Size: 552 2898 423 6371
Fragments arranged by size:
6371 2898 552 423
PmeI GTTT'AAAC
Cuts at: 7243 7243
Size: 10244
PmlI CAC'GTG
Cuts at: 3948 3948
Size: 10244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6228 6260 9383 9415 6228
Size: 32 3123 32 7057
Fragments arranged by size:
7057 3123 32 32
PpuMI rG'GwC_Cy
Cuts at: 225 1691 1829 2226 3593 6831 7066 225
Size: 1466 138 397 1367 3238 235 3403
Fragments arranged by size:
3403 3238 1466 1367 397 235 138
PspOMI G'GGCC_C
Cuts at: 1646 1739 1951 1987 2256 3294 3737 5796 6292
Size: 93 212 36 269 1038 443 2059 496
Cuts at: 6292 6580 6726 1646
Size: 288 146 5164
Fragments arranged by size:
5164 2059 1038 496 443 288 269 212
146 93 36
PspXI vC'TCGA_Gb
Cuts at: 3853 3853
Size: 10244
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1048 1080 1048
Size: 32 10212
PstI C_TGCA'G
Cuts at: 461 623 955 2663 3644 6389 7291 7976 461
Size: 162 332 1708 981 2745 902 685 2729
Fragments arranged by size:
2745 2729 1708 981 902 685 332 162
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10244
PvuII CAG'CTG
Cuts at: 640 801 1329 1400 1550 2105 2670 3066 3413
Size: 161 528 71 150 555 565 396 347
Cuts at: 3413 5139 5898 6926 7669 8029 640
Size: 1726 759 1028 743 360 2855
Fragments arranged by size:
2855 1726 1028 759 743 565 555 528
396 360 347 161 150 71
RsrII CG'GwC_CG
Cuts at: 8439 8439
Size: 10244
SacI G_AGCT'C
Cuts at: 2147 3544 5399 7258 2147
Size: 1397 1855 1859 5133
Fragments arranged by size:
5133 1859 1855 1397
SacII CC_GC'GG
Cuts at: 319 319
Size: 10244
SalI G'TCGA_C
Cuts at: 1177 3073 7281 1177
Size: 1896 4208 4140
Fragments arranged by size:
4208 4140 1896
SanDI GG'GwC_CC
Cuts at: 1691 2226 3593 1691
Size: 535 1367 8342
Fragments arranged by size:
8342 1367 535
SapI GCTCTTCn'nnn_
Cuts at: 2734 2977 3199 4411 8267 8477 2734
Size: 243 222 1212 3856 210 4501
Fragments arranged by size:
4501 3856 1212 243 222 210
SbfI CC_TGCA'GG
Cuts at: 461 7291 461
Size: 6830 3414
ScaI AGT'ACT
Cuts at: 607 1063 2338 9761 607
Size: 456 1275 7423 1090
Fragments arranged by size:
7423 1275 1090 456
SexAI A'CCwGG_T
Cuts at: 1424 1586 1424
Size: 162 10082
SfcI C'TryA_G
Cuts at: 33 457 619 951 1992 2659 3640 6385 6846
Size: 424 162 332 1041 667 981 2745 461
Cuts at: 6846 7287 7972 8965 9156 33
Size: 441 685 993 191 1121
Fragments arranged by size:
2745 1121 1041 993 981 685 667 461
441 424 332 191 162
SfiI GGCCn_nnn'nGGCC
Cuts at: 1654 5978 1654
Size: 4324 5920
SfoI GGC'GCC
Cuts at: 135 1107 1817 3727 6502 7676 7924 135
Size: 972 710 1910 2775 1174 248 2455
Fragments arranged by size:
2775 2455 1910 1174 972 710 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10244
SmaI CCC'GGG
Cuts at: 230 1654 1986 2122 3736 6601 7266 230
Size: 1424 332 136 1614 2865 665 3208
Fragments arranged by size:
3208 2865 1614 1424 665 332 136
SmlI C'TyrA_G
Cuts at: 297 1417 3853 7399 8314 8806 9068 297
Size: 1120 2436 3546 915 492 262 1473
Fragments arranged by size:
3546 2436 1473 1120 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1926 67
Size: 1859 8385
SphI G_CATG'C
Cuts at: 699 945 2718 2760 4236 5295 7297 7797 699
Size: 246 1773 42 1476 1059 2002 500 3146
Fragments arranged by size:
3146 2002 1773 1476 1059 500 246 42
SrfI GCCC'GGGC
Cuts at: 2122 3736 6601 2122
Size: 1614 2865 5765
Fragments arranged by size:
5765 2865 1614
StuI AGG'CCT
Cuts at: 463 1018 463
Size: 555 9689
StyI C'CwwG_G
Cuts at: 107 521 2690 3170 3989 4199 4521 4559 4988
Size: 414 2169 480 819 210 322 38 429
Cuts at: 4988 4994 5690 6527 6975 7062 7127 7136 7378
Size: 6 696 837 448 87 65 9 242
Cuts at: 7378 7732 107
Size: 354 2619
Fragments arranged by size:
2619 2169 837 819 696 480 448 429
414 354 322 242 210 87 65 38
9 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3144 3261 3471 3943 4560 4696 5852 7827 7960
Size: 117 210 472 617 136 1156 1975 133
Cuts at: 7960 8419 8628 3144
Size: 459 209 4760
Fragments arranged by size:
4760 1975 1156 617 472 459 210 209
136 133 117
TatI w'GTAC_w
Cuts at: 605 1061 1187 1430 2026 2336 4505 4652 9759
Size: 456 126 243 596 310 2169 147 5107
Cuts at: 9759 605
Size: 1090
Fragments arranged by size:
5107 2169 1090 596 456 310 243 147
126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 639 671 1881 1913 3577 3609 9761 9793 639
Size: 32 1210 32 1664 32 6152 32 1090
Fragments arranged by size:
6152 1664 1210 1090 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 8041 8041
Size: 10244
XbaI T'CTAG_A
Cuts at: 7275 7275
Size: 10244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1630 2280 2697 3276 3703 4941 6713 7134 7739
Size: 650 417 579 427 1238 1772 421 605
Cuts at: 7739 1630
Size: 4135
Fragments arranged by size:
4135 1772 1238 650 605 579 427 421
417
XhoI C'TCGA_G
Cuts at: 3853 3853
Size: 10244
XmaI C'CCGG_G
Cuts at: 228 1652 1984 2120 3734 6599 7264 228
Size: 1424 332 136 1614 2865 665 3208
Fragments arranged by size:
3208 2865 1614 1424 665 332 136
XmnI GAAnn'nnTTC
Cuts at: 4420 4420
Size: 10244
ZraI GAC'GTC
Cuts at: 6068 6068
Size: 10244
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BsiWI BsmI BssHII BstZ17I
Bsu36I ClaI CspCI EcoRV HindIII HpaI NdeI NotI
NruI PacI PshAI PsiI SgrAI SnaBI SspI SwaI