Restriction map for pF1KE2484
 (Linear) (Six-base) MAPSORT of: KIEE2484.seq  Check: 6334  from: 1  to: 7131



KIEE2484.orf

 With 182 enzymes: SgfI * 

                        July 18, 2019 20:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1363    3525    5467    7131 
   Size:       1363    2162    1942    1664

  Fragments arranged by size: 

                2162    1942    1664    1363
 
AatII G_ACGT'C

Cuts at:      0    5962    7131 
   Size:       5962    1169
 
AccI GT'mk_AC

Cuts at:      0    1070    2966    7131 
   Size:       1070    1896    4165

  Fragments arranged by size: 

                4165    1896    1070
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1825    2395    2545    2619    3376    3596    4213    4948 
   Size:       1825     570     150      74     757     220     617     735

Cuts at:   4948    5179    5188    5860    6748    7131 
   Size:        231       9     672     888     383

  Fragments arranged by size: 

                1825     888     757     735     672     617     570     383
                 231     220     150      74       9
 
AfeI AGC'GCT

Cuts at:      0     337     769    3294    3912    4749    7131 
   Size:        337     432    2525     618     837    2382

  Fragments arranged by size: 

                2525    2382     837     618     432     337
 
AflIII A'CryG_T

Cuts at:      0    4589    6155    7131 
   Size:       4589    1566     976

  Fragments arranged by size: 

                4589    1566     976
 
AgeI A'CCGG_T

Cuts at:      0    4379    7131 
   Size:       4379    2752
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3759    5451    7131 
   Size:       3759    1692    1680

  Fragments arranged by size: 

                3759    1692    1680
 
AleI CACnn'nnGTG

Cuts at:      0    5324    7131 
   Size:       5324    1807
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     658     868    2875    3572    7131 
   Size:        658     210    2007     697    3559

  Fragments arranged by size: 

                3559    2007     697     658     210
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3908    3940    7131 
   Size:       3908      32    3191

  Fragments arranged by size: 

                3908    3191      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1442    1654    2757    3229    3287    4191    4425    4914 
   Size:       1442     212    1103     472      58     904     234     489

Cuts at:   4914    5217    6203    6435    7131 
   Size:        303     986     232     696

  Fragments arranged by size: 

                1442    1103     986     904     696     489     472     303
                 234     232     212      58
 
ApaI G_GGCC'C

Cuts at:      0    1542    1635    1847    1883    2152    3190    3633    5692 
   Size:       1542      93     212      36     269    1038     443    2059

Cuts at:   5692    6188    6476    6622    7131 
   Size:        496     288     146     509

  Fragments arranged by size: 

                2059    1542    1038     509     496     443     288     269
                 212     146      93      36
 
ApaLI G'TGCA_C

Cuts at:      0    1483    1657    1777    3344    4821    7131 
   Size:       1483     174     120    1567    1477    2310

  Fragments arranged by size: 

                2310    1567    1483    1477     174     120
 
ApoI r'AATT_y

Cuts at:      0     735    2712    7131 
   Size:        735    1977    4419

  Fragments arranged by size: 

                4419    1977     735
 
AvaI C'yCGr_G

Cuts at:      0     120    1364    1411    1544    1876    2012    2302    3626 
   Size:        120    1244      47     133     332     136     290    1324

Cuts at:   3626    3745    5150    6491    6946    7131 
   Size:        119    1405    1341     455     185

  Fragments arranged by size: 

                1405    1341    1324    1244     455     332     290     185
                 136     133     120     119      47
 
BamHI G'GATC_C

Cuts at:      0     344    3716    7023    7131 
   Size:        344    3372    3307     108

  Fragments arranged by size: 

                3372    3307     344     108
 
BanI G'GyrC_C

Cuts at:      0      25     579     997    1707    1924    3537    3617    4108 
   Size:         25     554     418     710     217    1613      80     491

Cuts at:   4108    6334    6392    7131 
   Size:       2226      58     739

  Fragments arranged by size: 

                2226    1613     739     710     554     491     418     217
                  80      58      25
 
BanII G_rGCy'C

Cuts at:      0     802    1147    1286    1542    1635    1847    1883    2039 
   Size:        802     345     139     256      93     212      36     156

Cuts at:   2039    2152    2165    3008    3190    3436    3633    3726    5291 
   Size:        113      13     843     182     246     197      93    1565

Cuts at:   5291    5692    5724    5824    6188    6212    6476    6622    6710 
   Size:        401      32     100     364      24     264     146      88

Cuts at:   6710    7005    7131 
   Size:        295     126

  Fragments arranged by size: 

                1565     843     802     401     364     345     295     264
                 256     246     212     197     182     156     146     139
                 126     113     100      93      93      88      36      32
                  24      13
 
BbeI G_GCGC'C

Cuts at:      0      29    1001    1711    3621    6396    7131 
   Size:         29     972     710    1910    2775     735

  Fragments arranged by size: 

                2775    1910     972     735     710      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     654    2334    2584    4689    5632    6455    7131 
   Size:        654    1680     250    2105     943     823     676

  Fragments arranged by size: 

                2105    1680     943     823     676     654     250
 
BbvCI CC'TCA_GC

Cuts at:      0    4129    5632    7131 
   Size:       4129    1503    1499

  Fragments arranged by size: 

                4129    1503    1499
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2265    2299    3858    3892    4708    4742    7131 
   Size:       2265      34    1559      34     816      34    2389

  Fragments arranged by size: 

                2389    2265    1559     816      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     609    1778    4326    7131 
   Size:        609    1169    2548    2805

  Fragments arranged by size: 

                2805    2548    1169     609
 
BclI T'GATC_A

Cuts at:      0    1157    4172    7131 
   Size:       1157    3015    2959

  Fragments arranged by size: 

                3015    2959    1157
 
BfrBI ATG'CAT

Cuts at:      0    2652    7131 
   Size:       2652    4479
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1546    3461    5870    5909    6390    7131 
   Size:       1546    1915    2409      39     481     741

  Fragments arranged by size: 

                2409    1915    1546     741     481      39
 
BglII A'GATC_T

Cuts at:      0    1946    4931    6557    7131 
   Size:       1946    2985    1626     574

  Fragments arranged by size: 

                2985    1946    1626     574
 
BlpI GC'TnA_GC

Cuts at:      0     969    1393    4740    5606    6661    7131 
   Size:        969     424    3347     866    1055     470

  Fragments arranged by size: 

                3347    1055     969     866     470     424
 
Bme1580I G_kGCm'C

Cuts at:      0     584     857    1487    1542    1635    1661    1781    1847 
   Size:        584     273     630      55      93      26     120      66

Cuts at:   1847    1883    2013    2152    3190    3348    3633    4825    5692 
   Size:         36     130     139    1038     158     285    1192     867

Cuts at:   5692    6048    6188    6337    6476    6622    6853    7131 
   Size:        356     140     149     139     146     231     278

  Fragments arranged by size: 

                1192    1038     867     630     584     356     285     278
                 273     231     158     149     146     140     139     139
                 130     120      93      66      55      36      26
 
BmgBI CAC'GTC

Cuts at:      0    2807    7131 
   Size:       2807    4324
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    4003    4758    5508    5665    6618    7131 
   Size:       4003     755     750     157     953     513

  Fragments arranged by size: 

                4003     953     755     750     513     157
 
BmtI G_CTAG'C

Cuts at:      0    1274    7131 
   Size:       1274    5857
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2184    3450    3745    3771    7019    7131 
   Size:       2184    1266     295      26    3248     112

  Fragments arranged by size: 

                3248    2184    1266     295     112      26
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     784     906    1078    1927    1935    2205    2383 
   Size:        514     270     122     172     849       8     270     178

Cuts at:   2383    3306    3309    3511    4708    5095    5323    5581    5632 
   Size:        923       3     202    1197     387     228     258      51

Cuts at:   5632    5638    5788    5848    6070    6292    6608    7131 
   Size:          6     150      60     222     222     316     523

  Fragments arranged by size: 

                1197     923     849     523     514     387     316     270
                 270     258     228     222     222     202     178     172
                 150     122      60      51       8       6       3
 
Bpu10I CC'TnA_GC

Cuts at:      0     562     751    1355    1534    3688    4129    4165    5632 
   Size:        562     189     604     179    2154     441      36    1467

Cuts at:   5632    7131 
   Size:       1499

  Fragments arranged by size: 

                2154    1499    1467     604     562     441     189     179
                  36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210    1294    7131 
   Size:        210    1084    5837

  Fragments arranged by size: 

                5837    1084     210
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1192    1365    1417    4101    5291    6622    6671    7131 
   Size:       1192     173      52    2684    1190    1331      49     460

  Fragments arranged by size: 

                2684    1331    1192    1190     460     173      52      49
            
 
BsaAI yAC'GTr

Cuts at:      0    2859    3840    7131 
   Size:       2859     981    3291

  Fragments arranged by size: 

                3291    2859     981
 
BsaBI GATnn'nnATC

Cuts at:      0    1036    2254    7131 
   Size:       1036    1218    4877

  Fragments arranged by size: 

                4877    1218    1036
 
BsaHI Gr'CG_yC

Cuts at:      0      26     686     998    1615    1708    3618    3668    5959 
   Size:         26     660     312     617      93    1910      50    2291

Cuts at:   5959    6393    7131 
   Size:        434     738

  Fragments arranged by size: 

                2291    1910     738     660     617     434     312      93
                  50      26
 
BsaWI w'CCGG_w

Cuts at:      0    1794    2705    3809    4379    6992    7096    7131 
   Size:       1794     911    1104     570    2613     104      35

  Fragments arranged by size: 

                2613    1794    1104     911     570     104      35
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     204     234     904     934    1773    1803    4003    4033 
   Size:        204      30     670      30     839      30    2200      30

Cuts at:   4033    5935    5965    6817    6847    7131 
   Size:       1902      30     852      30     284

  Fragments arranged by size: 

                2200    1902     852     839     670     284     204      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22      25      46    1120    1315    2320    3442    3457 
   Size:         22       3      21    1074     195    1005    1122      15

Cuts at:   3457    3770    4663    4681    4795    5578    5602    6718    6810 
   Size:        313     893      18     114     783      24    1116      92

Cuts at:   6810    7011    7131 
   Size:        201     120

  Fragments arranged by size: 

                1122    1116    1074    1005     893     783     313     201
                 195     120     114      92      24      22      21      18
                  15       3
 
BseYI C'CCAG_C

Cuts at:      0     332     583     630     815    1064    1401    1427    1432 
   Size:        332     251      47     185     249     337      26       5

Cuts at:   1432    2099    2164    3022    3183    3259    3349    5639    5730 
   Size:        667      65     858     161      76      90    2290      91

Cuts at:   5730    5899    6203    6251    6973    7113    7131 
   Size:        169     304      48     722     140      18

  Fragments arranged by size: 

                2290     858     722     667     337     332     304     251
                 249     185     169     161     140      91      90      76
                  65      48      47      26      18       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    4312    5710    7131 
   Size:       4312    1398    1421

  Fragments arranged by size: 

                4312    1421    1398
 
BsiEI CG_ry'CG

Cuts at:      0      94     559    2046    3131    3342    7131 
   Size:         94     465    1487    1085     211    3789

  Fragments arranged by size: 

                3789    1487    1085     465     211      94
 
BsiHKAI G_wGCw'C

Cuts at:      0     983    1487    1661    1781    2039    2573    2864    3083 
   Size:        983     504     174     120     258     534     291     219

Cuts at:   3083    3160    3348    3436    3587    4064    4825    4895    5291 
   Size:         77     188      88     151     477     761      70     396

Cuts at:   5291    5774    6175    7131 
   Size:        483     401     956

  Fragments arranged by size: 

                 983     956     761     534     504     483     477     401
                 396     291     258     219     188     174     151     120
                  88      77      70
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1792    4201    7131 
   Size:       1792    2409    2930

  Fragments arranged by size: 

                2930    2409    1792
 
Bsp1286I G_dGCh'C

Cuts at:      0     584     802     857     983    1147    1286    1487    1542 
   Size:        584     218      55     126     164     139     201      55

Cuts at:   1542    1635    1661    1781    1847    1883    2013    2039    2152 
   Size:         93      26     120      66      36     130      26     113

Cuts at:   2152    2165    2573    2864    3008    3083    3160    3190    3348 
   Size:         13     408     291     144      75      77      30     158

Cuts at:   3348    3436    3587    3633    3726    4064    4825    4895    5291 
   Size:         88     151      46      93     338     761      70     396

Cuts at:   5291    5692    5724    5774    5824    6048    6175    6188    6212 
   Size:        401      32      50      50     224     127      13      24

Cuts at:   6212    6337    6476    6622    6710    6853    7005    7131 
   Size:        125     139     146      88     143     152     126

  Fragments arranged by size: 

                 761     584     408     401     396     338     291     224
                 218     201     164     158     152     151     146     144
                 143     139     139     130     127     126     126     125
                 120     113      93      93      88      88      77      75
                  70      66      55      55      50      50      46      36
                  32      30      26      26      24      13      13
 
BspEI T'CCGG_A

Cuts at:      0    6992    7131 
   Size:       6992     139
 
BspHI T'CATG_A

Cuts at:      0    1154    2246    2573    2840    4517    5129    7131 
   Size:       1154    1092     327     267    1677     612    2002

  Fragments arranged by size: 

                2002    1677    1154    1092     612     327     267
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     518    1363    3525    3527    4031    5467    6461    7131 
   Size:        518     845    2162       2     504    1436     994     670

  Fragments arranged by size: 

                2162    1436     994     845     670     518     504       2
            
 
BsrBI CCG'CTC

Cuts at:      0      22     561    2682    3381    3447    3495    4164    7131 
   Size:         22     539    2121     699      66      48     669    2967

  Fragments arranged by size: 

                2967    2121     699     669     539      66      48      22
            
 
BsrDI GCAATG_nn'

Cuts at:      0    1530    2831    3603    5144    7131 
   Size:       1530    1301     772    1541    1987

  Fragments arranged by size: 

                1987    1541    1530    1301     772
 
BsrFI r'CCGG_y

Cuts at:      0      89     458    1589    2129    2544    4379    6767    6899 
   Size:         89     369    1131     540     415    1835    2388     132

Cuts at:   6899    7131 
   Size:        232

  Fragments arranged by size: 

                2388    1835    1131     540     415     369     232     132
                  89
 
BsrGI T'GTAC_A

Cuts at:      0    1322    7131 
   Size:       1322    5809
 
BssSI C'ACGA_G

Cuts at:      0    2293    3241    7131 
   Size:       2293     948    3890

  Fragments arranged by size: 

                3890    2293     948
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1239    1442    1902    5217    6138    7131 
   Size:       1239     203     460    3315     921     993

  Fragments arranged by size: 

                3315    1239     993     921     460     203
 
BstEII G'GTnAC_C

Cuts at:      0    4875    7131 
   Size:       4875    2256
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1266    2889    3882    4119    4887    6883    7131 
   Size:       1266    1623     993     237     768    1996     248

  Fragments arranged by size: 

                1996    1623    1266     993     768     248     237
 
BstYI r'GATC_y

Cuts at:      0     344    1559    1946    2753    3716    4115    4931    5924 
   Size:        344    1215     387     807     963     399     816     993

Cuts at:   5924    6557    7023    7131 
   Size:        633     466     108

  Fragments arranged by size: 

                1215     993     963     816     807     633     466     399
                 387     344     108
 
BtgI C'CryG_G

Cuts at:      0     208     386    2582    3881    4091    4880    4886    5777 
   Size:        208     178    2196    1299     210     789       6     891

Cuts at:   5777    6074    6718    7131 
   Size:        297     644     413

  Fragments arranged by size: 

                2196    1299     891     789     644     413     297     210
                 208     178       6
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     303     465    3457    3535    3861    7131 
   Size:        303     162    2992      78     326    3270

  Fragments arranged by size: 

                3270    2992     326     303     162      78
 
BtsI GCAGTG_nn'

Cuts at:      0    3471    3529    3554    6173    7131 
   Size:       3471      58      25    2619     958

  Fragments arranged by size: 

                3471    2619     958      58      25
 
DraIII CAC_nnn'GTG

Cuts at:      0    1482    7131 
   Size:       1482    5649
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3841    4215    7131 
   Size:       3841     374    2916

  Fragments arranged by size: 

                3841    2916     374
 
EaeI y'GGCC_r

Cuts at:      0      91     304    2235    2594    3128    3339    3564    4416 
   Size:         91     213    1931     359     534     211     225     852

Cuts at:   4416    4883    5039    6696    7131 
   Size:        467     156    1657     435

  Fragments arranged by size: 

                1931    1657     852     534     467     435     359     225
                 213     211     156      91
 
EagI C'GGCC_G

Cuts at:      0      91    3128    3339    7131 
   Size:         91    3037     211    3792

  Fragments arranged by size: 

                3792    3037     211      91
 
EarI CTCTTCn'nnn_

Cuts at:      0    2626    2662    2869    3091    3419    3434    4303    4640 
   Size:       2626      36     207     222     328      15     869     337

Cuts at:   4640    4849    5251    5389    7131 
   Size:        209     402     138    1742

  Fragments arranged by size: 

                2626    1742     869     402     337     328     222     209
                 207     138      36      15
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     157    2497    2920    3784    7131 
   Size:        157    2340     423     864    3347

  Fragments arranged by size: 

                3347    2340     864     423     157
 
EcoICRI GAG'CTC

Cuts at:      0    2037    3434    5289    7131 
   Size:       2037    1397    1855    1842

  Fragments arranged by size: 

                2037    1855    1842    1397
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     784     906    1078    1825    1927    1935    2205 
   Size:        514     270     122     172     747     102       8     270

Cuts at:   2205    2383    2395    2545    2619    3306    3309    3376    3511 
   Size:        178      12     150      74     687       3      67     135

Cuts at:   3511    3596    4213    4708    4948    5095    5179    5188    5323 
   Size:         85     617     495     240     147      84       9     135

Cuts at:   5323    5581    5632    5638    5788    5848    5860    6070    6292 
   Size:        258      51       6     150      60      12     210     222

Cuts at:   6292    6608    6748    7131 
   Size:        316     140     383

  Fragments arranged by size: 

                 747     687     617     514     495     383     316     270
                 270     258     240     222     210     178     172     150
                 150     147     140     135     135     122     102      85
                  84      74      67      60      51      12      12       9
                   8       6       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1333    2184    2761    3175    4417    5221    6590    7131 
   Size:       1333     851     577     414    1242     804    1369     541

  Fragments arranged by size: 

                1369    1333    1242     851     804     577     541     414
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     117    1538    1539    1583    1631    1632    1721    1844 
   Size:        117    1421       1      44      48       1      89     123

Cuts at:   1844    1880    2118    3187    3485    3630    4422    5214    5331 
   Size:         36     238    1069     298     145     792     792     117

Cuts at:   5331    5688    5689    6184    6185    6473    6618    6619    6723 
   Size:        357       1     495       1     288     145       1     104

Cuts at:   6723    6889    6958    7131 
   Size:        166      69     173

  Fragments arranged by size: 

                1421    1069     792     792     495     357     298     288
                 238     173     166     145     145     123     117     117
                 104      89      69      48      44      36       1       1
                   1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3072    3528    4419    7044    7131 
   Size:       3072     456     891    2625      87

  Fragments arranged by size: 

                3072    2625     891     456      87
 
FseI GG_CCGG'CC

Cuts at:      0      93    7131 
   Size:         93    7038
 
FspI TGC'GCA

Cuts at:      0     592    5143    7131 
   Size:        592    4551    1988

  Fragments arranged by size: 

                4551    1988     592
 
FspAI rTGC'GCAy

Cuts at:      0     592    7131 
   Size:        592    6539
 
HaeII r_GCGC'y

Cuts at:      0      29     166     339     771    1001    1711    2551    3184 
   Size:         29     137     173     432     230     710     840     633

Cuts at:   3184    3266    3296    3321    3621    3891    3914    4677    4751 
   Size:         82      30      25     300     270      23     763      74

Cuts at:   4751    5194    6323    6396    6768    7131 
   Size:        443    1129      73     372     363

  Fragments arranged by size: 

                1129     840     763     710     633     443     432     372
                 363     300     270     230     173     137      82      74
                  73      30      29      25      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     204     236    1080    1112    3001    3033    3235    3267 
   Size:        204      32     844      32    1889      32     202      32

Cuts at:   3267    3370    3402    3745    3777    4689    4721    5437    5469 
   Size:        103      32     343      32     912      32     716      32

Cuts at:   5469    5788    5820    5931    5935    5956    5963    5967    5988 
   Size:        319      32     111       4      21       7       4      21

Cuts at:   5988    6697    6729    6791    6817    6823    6849    6942    6974 
   Size:        709      32      62      26       6      26      93      32

Cuts at:   6974    7131 
   Size:        157

  Fragments arranged by size: 

                1889     912     844     716     709     343     319     204
                 202     157     111     103      93      62      32      32
                  32      32      32      32      32      32      32      32
                  32      26      26      21      21       7       6       4
                   4
 
HincII GTy'rAC

Cuts at:      0     942    1071    2265    2967    5091    5529    7131 
   Size:        942     129    1194     702    2124     438    1602

  Fragments arranged by size: 

                2124    1602    1194     942     702     438     129
 
KasI G'GCGC_C

Cuts at:      0      25     997    1707    3617    6392    7131 
   Size:         25     972     710    1910    2775     739

  Fragments arranged by size: 

                2775    1910     972     739     710      25
 
MfeI C'AATT_G

Cuts at:      0    5406    7131 
   Size:       5406    1725
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1277    4015    4808    5939    6629    7131 
   Size:       1277    2738     793    1131     690     502

  Fragments arranged by size: 

                2738    1277    1131     793     690     502
 
MscI TGG'CCA

Cuts at:      0    2596    4418    4885    5041    7131 
   Size:       2596    1822     467     156    2090

  Fragments arranged by size: 

                2596    2090    1822     467     156
 
MslI CAynn'nnrTG

Cuts at:      0     259    1138    2452    2887    3139    3880    4084    4594 
   Size:        259     879    1314     435     252     741     204     510

Cuts at:   4594    4885    5134    5206    5324    7131 
   Size:        291     249      72     118    1807

  Fragments arranged by size: 

                1807    1314     879     741     510     435     291     259
                 252     249     204     118      72
 
MspA1I CmG'CkG

Cuts at:      0     210     532     693     973    1209    1221    1292    1442 
   Size:        210     322     161     280     236      12      71     150

Cuts at:   1442    1700    1757    1997    2556    2562    2958    3215    3305 
   Size:        258      57     240     559       6     396     257      90

Cuts at:   3305    4106    5031    5734    5790    6818    6830    7131 
   Size:        801     925     703      56    1028      12     301

  Fragments arranged by size: 

                1028     925     801     703     559     396     322     301
                 280     258     257     240     236     210     161     150
                  90      71      57      56      12      12       6
 
NaeI GCC'GGC

Cuts at:      0      91    1591    2131    2546    6769    6901    7131 
   Size:         91    1500     540     415    4223     132     230

  Fragments arranged by size: 

                4223    1500     540     415     230     132      91
 
NarI GG'CG_CC

Cuts at:      0      26     998    1708    3618    6393    7131 
   Size:         26     972     710    1910    2775     738

  Fragments arranged by size: 

                2775    1910     972     738     710      26
 
NcoI C'CATG_G

Cuts at:      0    2582    3881    4091    4880    4886    7131 
   Size:       2582    1299     210     789       6    2245

  Fragments arranged by size: 

                2582    2245    1299     789     210       6
 
NgoMIV G'CCGG_C

Cuts at:      0      89    1589    2129    2544    6767    6899    7131 
   Size:         89    1500     540     415    4223     132     232

  Fragments arranged by size: 

                4223    1500     540     415     232     132      89
 
NheI G'CTAG_C

Cuts at:      0    1270    7131 
   Size:       1270    5861
 
NsiI A_TGCA'T

Cuts at:      0    2654    7131 
   Size:       2654    4477
 
NspI r_CATG'y

Cuts at:      0     264     300     591     837    2610    2652    4128    4593 
   Size:        264      36     291     246    1773      42    1476     465

Cuts at:   4593    5187    6027    6159    7131 
   Size:        594     840     132     972

  Fragments arranged by size: 

                1773    1476     972     840     594     465     291     264
                 246     132      42      36
 
PasI CC'CwG_GG

Cuts at:      0     291    2973    3072    5866    6189    7131 
   Size:        291    2682      99    2794     323     942

  Fragments arranged by size: 

                2794    2682     942     323     291      99
 
PciI A'CATG_T

Cuts at:      0    4589    6155    7131 
   Size:       4589    1566     976

  Fragments arranged by size: 

                4589    1566     976
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1479    3658    4836    4914    7131 
   Size:       1479    2179    1178      78    2217

  Fragments arranged by size: 

                2217    2179    1479    1178      78
 
PfoI T'CCnGG_A

Cuts at:      0    1562    2114    5012    5435    7131 
   Size:       1562     552    2898     423    1696

  Fragments arranged by size: 

                2898    1696    1562     552     423
 
PmlI CAC'GTG

Cuts at:      0    3840    7131 
   Size:       3840    3291
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    6120    6152    7131 
   Size:       6120      32     979

  Fragments arranged by size: 

                6120     979      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     117    1583    1721    2118    3485    6723    6958    7131 
   Size:        117    1466     138     397    1367    3238     235     173

  Fragments arranged by size: 

                3238    1466    1367     397     235     173     138     117
            
 
PspOMI G'GGCC_C

Cuts at:      0    1538    1631    1843    1879    2148    3186    3629    5688 
   Size:       1538      93     212      36     269    1038     443    2059

Cuts at:   5688    6184    6472    6618    7131 
   Size:        496     288     146     513

  Fragments arranged by size: 

                2059    1538    1038     513     496     443     288     269
                 212     146      93      36
 
PspXI vC'TCGA_Gb

Cuts at:      0    3745    7131 
   Size:       3745    3386
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     940     972    7131 
   Size:        940      32    6159

  Fragments arranged by size: 

                6159     940      32
 
PstI C_TGCA'G

Cuts at:      0     353     515     847    2555    3536    6281    7131 
   Size:        353     162     332    1708     981    2745     850

  Fragments arranged by size: 

                2745    1708     981     850     353     332     162
 
PvuII CAG'CTG

Cuts at:      0     532     693    1221    1292    1442    1997    2562    2958 
   Size:        532     161     528      71     150     555     565     396

Cuts at:   2958    3305    5031    5790    6818    7131 
   Size:        347    1726     759    1028     313

  Fragments arranged by size: 

                1726    1028     759     565     555     532     528     396
                 347     313     161     150      71
 
SacI G_AGCT'C

Cuts at:      0    2039    3436    5291    7131 
   Size:       2039    1397    1855    1840

  Fragments arranged by size: 

                2039    1855    1840    1397
 
SacII CC_GC'GG

Cuts at:      0     211    7131 
   Size:        211    6920
 
SalI G'TCGA_C

Cuts at:      0    1069    2965    7131 
   Size:       1069    1896    4166

  Fragments arranged by size: 

                4166    1896    1069
 
SanDI GG'GwC_CC

Cuts at:      0    1583    2118    3485    7131 
   Size:       1583     535    1367    3646

  Fragments arranged by size: 

                3646    1583    1367     535
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2626    2869    3091    4303    7131 
   Size:       2626     243     222    1212    2828

  Fragments arranged by size: 

                2828    2626    1212     243     222
 
SbfI CC_TGCA'GG

Cuts at:      0     353    7131 
   Size:        353    6778
 
ScaI AGT'ACT

Cuts at:      0     499     955    2230    7131 
   Size:        499     456    1275    4901

  Fragments arranged by size: 

                4901    1275     499     456
 
SexAI A'CCwGG_T

Cuts at:      0    1316    1478    7131 
   Size:       1316     162    5653

  Fragments arranged by size: 

                5653    1316     162
 
SfcI C'TryA_G

Cuts at:      0     349     511     843    1884    2551    3532    6277    6738 
   Size:        349     162     332    1041     667     981    2745     461

Cuts at:   6738    7131 
   Size:        393

  Fragments arranged by size: 

                2745    1041     981     667     461     393     349     332
                 162
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1546    5870    7131 
   Size:       1546    4324    1261

  Fragments arranged by size: 

                4324    1546    1261
 
SfoI GGC'GCC

Cuts at:      0      27     999    1709    3619    6394    7131 
   Size:         27     972     710    1910    2775     737

  Fragments arranged by size: 

                2775    1910     972     737     710      27
 
SmaI CCC'GGG

Cuts at:      0     122    1546    1878    2014    3628    6493    7131 
   Size:        122    1424     332     136    1614    2865     638

  Fragments arranged by size: 

                2865    1614    1424     638     332     136     122
 
SmlI C'TyrA_G

Cuts at:      0     189    1309    3745    7131 
   Size:        189    1120    2436    3386

  Fragments arranged by size: 

                3386    2436    1120     189
 
SpeI A'CTAG_T

Cuts at:      0    1818    7131 
   Size:       1818    5313
 
SphI G_CATG'C

Cuts at:      0     591     837    2610    2652    4128    5187    7131 
   Size:        591     246    1773      42    1476    1059    1944

  Fragments arranged by size: 

                1944    1773    1476    1059     591     246      42
 
SrfI GCCC'GGGC

Cuts at:      0    2014    3628    6493    7131 
   Size:       2014    1614    2865     638

  Fragments arranged by size: 

                2865    2014    1614     638
 
StuI AGG'CCT

Cuts at:      0     355     910    7131 
   Size:        355     555    6221

  Fragments arranged by size: 

                6221     555     355
 
StyI C'CwwG_G

Cuts at:      0     413    2582    3062    3881    4091    4413    4451    4880 
   Size:        413    2169     480     819     210     322      38     429

Cuts at:   4880    4886    5582    6419    6867    6954    7019    7028    7131 
   Size:          6     696     837     448      87      65       9     103

  Fragments arranged by size: 

                2169     837     819     696     480     448     429     413
                 322     210     103      87      65      38       9       6
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3036    3153    3363    3835    4452    4588    5744    7131 
   Size:       3036     117     210     472     617     136    1156    1387

  Fragments arranged by size: 

                3036    1387    1156     617     472     210     136     117
            
 
TatI w'GTAC_w

Cuts at:      0     497     953    1079    1322    1918    2228    4397    4544 
   Size:        497     456     126     243     596     310    2169     147

Cuts at:   4544    7131 
   Size:       2587

  Fragments arranged by size: 

                2587    2169     596     497     456     310     243     147
                 126
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     531     563    1773    1805    3469    3501    7131 
   Size:        531      32    1210      32    1664      32    3630

  Fragments arranged by size: 

                3630    1664    1210     531      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1522    2172    2589    3168    3595    4833    6605    7026 
   Size:       1522     650     417     579     427    1238    1772     421

Cuts at:   7026    7131 
   Size:        105

  Fragments arranged by size: 

                1772    1522    1238     650     579     427     421     417
                 105
 
XhoI C'TCGA_G

Cuts at:      0    3745    7131 
   Size:       3745    3386
 
XmaI C'CCGG_G

Cuts at:      0     120    1544    1876    2012    3626    6491    7131 
   Size:        120    1424     332     136    1614    2865     640

  Fragments arranged by size: 

                2865    1614    1424     640     332     136     120
 
XmnI GAAnn'nnTTC

Cuts at:      0    4312    7131 
   Size:       4312    2819
 
ZraI GAC'GTC

Cuts at:      0    5960    7131 
   Size:       5960    1171
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     KasI     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI    PspXI
     PsrI     PstI    PvuII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI
     SpeI     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AscI     AseI    AsiSI    AvrII     BaeI
    BsiWI     BsmI   BssHII    BstBI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI    EcoRI    EcoRV  HindIII     HpaI     KpnI     MluI     NdeI
     NotI     NruI     PacI     PmeI    PshAI     PsiI     PvuI    RsrII
     SgfI    SgrAI    SnaBI     SspI     SwaI  Tth111I     XbaI
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