Restriction map for pF1KE5816
 (Linear) (Six-base) MAPSORT of: KIEE5816.seq  Check: 2391  from: 1  to: 2586



KIEE5816.orf

 With 182 enzymes: SgfI * 

                        July 26, 2013 11:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     618    1269    1501    1583    1913    2446    2586 
   Size:        313     305     651     232      82     330     533     140

  Fragments arranged by size: 

                 651     533     330     313     305     232     140      82
            
 
AfeI AGC'GCT

Cuts at:      0     136    2586 
   Size:        136    2450
 
AflIII A'CryG_T

Cuts at:      0      92    2586 
   Size:         92    2494
 
AleI CACnn'nnGTG

Cuts at:      0    1978    2586 
   Size:       1978     608
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2047    2079    2586 
   Size:       2047      32     507

  Fragments arranged by size: 

                2047     507      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     408     435    2586 
   Size:        408      27    2151

  Fragments arranged by size: 

                2151     408      27
 
ApaI G_GGCC'C

Cuts at:      0    1700    2586 
   Size:       1700     886
 
ApaLI G'TGCA_C

Cuts at:      0     538     606    2586 
   Size:        538      68    1980

  Fragments arranged by size: 

                1980     538      68
 
ApoI r'AATT_y

Cuts at:      0     772    1051    1927    2247    2254    2289    2586 
   Size:        772     279     876     320       7      35     297

  Fragments arranged by size: 

                 876     772     320     297     279      35       7
 
AvaI C'yCGr_G

Cuts at:      0     124    2069    2586 
   Size:        124    1945     517

  Fragments arranged by size: 

                1945     517     124
 
BamHI G'GATC_C

Cuts at:      0      81    2586 
   Size:         81    2505
 
BanI G'GyrC_C

Cuts at:      0     109     559    1816    2586 
   Size:        109     450    1257     770

  Fragments arranged by size: 

                1257     770     450     109
 
BanII G_rGCy'C

Cuts at:      0     734    1150    1628    1700    2586 
   Size:        734     416     478      72     886

  Fragments arranged by size: 

                 886     734     478     416      72
 
BbeI G_GCGC'C

Cuts at:      0     563    2586 
   Size:        563    2023
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     713    2269    2586 
   Size:        713    1556     317

  Fragments arranged by size: 

                1556     713     317
 
BbvCI CC'TCA_GC

Cuts at:      0      49    2586 
   Size:         49    2537
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     436     470    2586 
   Size:        436      34    2116

  Fragments arranged by size: 

                2116     436      34
 
BfrBI ATG'CAT

Cuts at:      0    1216    2586 
   Size:       1216    1370
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1492    2586 
   Size:       1492    1094
 
BglII A'GATC_T

Cuts at:      0    1706    2586 
   Size:       1706     880
 
BlpI GC'TnA_GC

Cuts at:      0     415     733    1648    2586 
   Size:        415     318     915     938

  Fragments arranged by size: 

                 938     915     415     318
 
Bme1580I G_kGCm'C

Cuts at:      0     112     542     610    1700    2586 
   Size:        112     430      68    1090     886

  Fragments arranged by size: 

                1090     886     430     112      68
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1673    2095    2586 
   Size:       1673     422     491

  Fragments arranged by size: 

                1673     491     422
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     748    2433    2586 
   Size:        208     540    1685     153

  Fragments arranged by size: 

                1685     540     208     153
 
Bpu10I CC'TnA_GC

Cuts at:      0      49    2586 
   Size:         49    2537
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538    2468    2491    2586 
   Size:        538    1930      23      95

  Fragments arranged by size: 

                1930     538      95      23
 
BsaAI yAC'GTr

Cuts at:      0    1398    1506    1518    2586 
   Size:       1398     108      12    1068

  Fragments arranged by size: 

                1398    1068     108      12
 
BsaHI Gr'CG_yC

Cuts at:      0     121     560    2586 
   Size:        121     439    2026

  Fragments arranged by size: 

                2026     439     121
 
BsaWI w'CCGG_w

Cuts at:      0     176    2586 
   Size:        176    2410
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     142     145     205    1158    1393    2053    2586 
   Size:        142       3      60     953     235     660     533

  Fragments arranged by size: 

                 953     660     533     235     142      60       3
 
BseYI C'CCAG_C

Cuts at:      0    1830    2586 
   Size:       1830     756
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1140    1387    1519    1540    2586 
   Size:       1140     247     132      21    1046

  Fragments arranged by size: 

                1140    1046     247     132      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     269     542     610     734    2586 
   Size:        269     273      68     124    1852

  Fragments arranged by size: 

                1852     273     269     124      68
 
BsmI GAATG_Cn'

Cuts at:      0    1218    1366    2586 
   Size:       1218     148    1220

  Fragments arranged by size: 

                1220    1218     148
 
Bsp1286I G_dGCh'C

Cuts at:      0     112     269     542     610     734    1150    1628    1700 
   Size:        112     157     273      68     124     416     478      72

Cuts at:   1700    2586 
   Size:        886

  Fragments arranged by size: 

                 886     478     416     273     157     124     112      72
                  68
 
BspEI T'CCGG_A

Cuts at:      0     176    2586 
   Size:        176    2410
 
BspHI T'CATG_A

Cuts at:      0    1154    1778    2348    2586 
   Size:       1154     624     570     238

  Fragments arranged by size: 

                1154     624     570     238
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1424    2124    2586 
   Size:       1424     700     462

  Fragments arranged by size: 

                1424     700     462
 
BsrBI CCG'CTC

Cuts at:      0     573    2586 
   Size:        573    2013
 
BsrDI GCAATG_nn'

Cuts at:      0     606     811    1661    2586 
   Size:        606     205     850     925

  Fragments arranged by size: 

                 925     850     606     205
 
BsrFI r'CCGG_y

Cuts at:      0      35    2586 
   Size:         35    2551
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     546     605     993    2586 
   Size:        546      59     388    1593

  Fragments arranged by size: 

                1593     546     388      59
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1446    2586 
   Size:       1446    1140
 
BstYI r'GATC_y

Cuts at:      0      81    1706    2586 
   Size:         81    1625     880

  Fragments arranged by size: 

                1625     880      81
 
Bsu36I CC'TnA_GG

Cuts at:      0    1141    2560    2586 
   Size:       1141    1419      26

  Fragments arranged by size: 

                1419    1141      26
 
BtgI C'CryG_G

Cuts at:      0     254    1387    1487    1976    2586 
   Size:        254    1133     100     489     610

  Fragments arranged by size: 

                1133     610     489     254     100
 
BtsI GCAGTG_nn'

Cuts at:      0     516    2586 
   Size:        516    2070
 
DraI TTT'AAA

Cuts at:      0     872    1860    2586 
   Size:        872     988     726

  Fragments arranged by size: 

                 988     872     726
 
EaeI y'GGCC_r

Cuts at:      0     251     335    2586 
   Size:        251      84    2251

  Fragments arranged by size: 

                2251     251      84
 
EarI CTCTTCn'nnn_

Cuts at:      0     848    2586 
   Size:        848    1738
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1541    2586 
   Size:       1541    1045
 
EcoICRI GAG'CTC

Cuts at:      0     732    2586 
   Size:        732    1854
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     313     618     748    1269    1501    1583    1913 
   Size:        208     105     305     130     521     232      82     330

Cuts at:   1913    2433    2446    2586 
   Size:        520      13     140

  Fragments arranged by size: 

                 521     520     330     305     232     208     140     130
                 105      82      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1578    2586 
   Size:       1578    1008
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1697    2586 
   Size:       1697     889
 
EcoRI G'AATT_C

Cuts at:      0    2247    2289    2586 
   Size:       2247      42     297

  Fragments arranged by size: 

                2247     297      42
 
EcoRV GAT'ATC

Cuts at:      0    1734    2445    2586 
   Size:       1734     711     141

  Fragments arranged by size: 

                1734     711     141
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1356    2586 
   Size:       1356    1230
 
FspI TGC'GCA

Cuts at:      0     358    2586 
   Size:        358    2228
 
FspAI rTGC'GCAy

Cuts at:      0     358    2586 
   Size:        358    2228
 
HaeII r_GCGC'y

Cuts at:      0      36     138     408     563    2586 
   Size:         36     102     270     155    2023

  Fragments arranged by size: 

                2023     270     155     102      36
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2464    2496    2586 
   Size:       2464      32      90

  Fragments arranged by size: 

                2464      90      32
 
HincII GTy'rAC

Cuts at:      0    1033    2586 
   Size:       1033    1553
 
KasI G'GCGC_C

Cuts at:      0     559    2586 
   Size:        559    2027
 
MfeI C'AATT_G

Cuts at:      0     991    2586 
   Size:        991    1595
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     926    2586 
   Size:        926    1660
 
MscI TGG'CCA

Cuts at:      0     337    2586 
   Size:        337    2249
 
MslI CAynn'nnrTG

Cuts at:      0     169    1444    1978    2586 
   Size:        169    1275     534     608

  Fragments arranged by size: 

                1275     608     534     169
 
MspA1I CmG'CkG

Cuts at:      0     249     423     621    1500    2586 
   Size:        249     174     198     879    1086

  Fragments arranged by size: 

                1086     879     249     198     174
 
NaeI GCC'GGC

Cuts at:      0      37    2586 
   Size:         37    2549
 
NarI GG'CG_CC

Cuts at:      0     560    2586 
   Size:        560    2026
 
NcoI C'CATG_G

Cuts at:      0    1976    2586 
   Size:       1976     610
 
NgoMIV G'CCGG_C

Cuts at:      0      35    2586 
   Size:         35    2551
 
NsiI A_TGCA'T

Cuts at:      0    1218    2586 
   Size:       1218    1368
 
NspI r_CATG'y

Cuts at:      0      30      96    2001    2244    2586 
   Size:         30      66    1905     243     342

  Fragments arranged by size: 

                1905     342     243      66      30
 
PciI A'CATG_T

Cuts at:      0      92    2586 
   Size:         92    2494
 
PfoI T'CCnGG_A

Cuts at:      0      77     140    1309    2586 
   Size:         77      63    1169    1277

  Fragments arranged by size: 

                1277    1169      77      63
 
PmlI CAC'GTG

Cuts at:      0    1518    2586 
   Size:       1518    1068
 
PsiI TTA'TAA

Cuts at:      0    1967    2586 
   Size:       1967     619
 
PspOMI G'GGCC_C

Cuts at:      0    1696    2586 
   Size:       1696     890
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1930    1962    2586 
   Size:       1930      32     624

  Fragments arranged by size: 

                1930     624      32
 
PstI C_TGCA'G

Cuts at:      0     248    1706    2586 
   Size:        248    1458     880

  Fragments arranged by size: 

                1458     880     248
 
PvuII CAG'CTG

Cuts at:      0     249    1500    2586 
   Size:        249    1251    1086

  Fragments arranged by size: 

                1251    1086     249
 
SacI G_AGCT'C

Cuts at:      0     734    2586 
   Size:        734    1852
 
SfcI C'TryA_G

Cuts at:      0     244    1702    2586 
   Size:        244    1458     884

  Fragments arranged by size: 

                1458     884     244
 
SfoI GGC'GCC

Cuts at:      0     561    2586 
   Size:        561    2025
 
SmlI C'TyrA_G

Cuts at:      0     553    2470    2483    2586 
   Size:        553    1917      13     103

  Fragments arranged by size: 

                1917     553     103      13
 
SphI G_CATG'C

Cuts at:      0    2244    2586 
   Size:       2244     342
 
StuI AGG'CCT

Cuts at:      0    1082    2586 
   Size:       1082    1504
 
StyI C'CwwG_G

Cuts at:      0    1976    2586 
   Size:       1976     610
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1238    2586 
   Size:       1238    1348
 
TatI w'GTAC_w

Cuts at:      0    1799    2586 
   Size:       1799     787
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1576    1608    2586 
   Size:       1576      32     978

  Fragments arranged by size: 

                1576     978      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     712    2407    2586 
   Size:        712    1695     179

  Fragments arranged by size: 

                1695     712     179
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    FspAI    HaeII    Hin4I   HincII     KasI
     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NsiI     NspI     PciI     PfoI     PmlI     PsiI   PspOMI
     PsrI     PstI    PvuII     SacI     SfcI     SfoI     SmlI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AlfI     AscI     AseI    AsiSI    AvrII     BaeI    BciVI     BclI
    BmgBI     BmrI     BmtI     BsaI    BsaBI    BsaXI    BsiEI    BsiWI
    BsmBI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI   DraIII     DrdI     EagI     FseI  HindIII     HpaI
     KpnI     MluI     NdeI     NheI     NotI     NruI     PacI     PasI
    PflMI     PmeI     PpiI    PpuMI    PshAI    PspXI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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