Restriction map for pF1KB5943
 (Linear) (Six-base) MAPSORT of: KIBB5943.seq  Check: 9458  from: 1  to: 1524



KIBB5943.orf

 With 182 enzymes: SgfI * 

                        September 25, 2012 15:36  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     725     799    1524 
   Size:        725      74     725

  Fragments arranged by size: 

                 725     725      74
 
AgeI A'CCGG_T

Cuts at:      0     345    1524 
   Size:        345    1179
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      74    1524 
   Size:         74    1450
 
AlwNI CAG_nnn'CTG

Cuts at:      0     586    1524 
   Size:        586     938
 
ApoI r'AATT_y

Cuts at:      0      91     638     850    1472    1524 
   Size:         91     547     212     622      52

  Fragments arranged by size: 

                 622     547     212      91      52
 
AvaI C'yCGr_G

Cuts at:      0     237    1524 
   Size:        237    1287
 
BamHI G'GATC_C

Cuts at:      0       3    1524 
   Size:          3    1521
 
BanI G'GyrC_C

Cuts at:      0      58    1524 
   Size:         58    1466
 
BanII G_rGCy'C

Cuts at:      0      31     200     302    1524 
   Size:         31     169     102    1222

  Fragments arranged by size: 

                1222     169     102      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     102     427     560     787     896    1524 
   Size:        102     325     133     227     109     628

  Fragments arranged by size: 

                 628     325     227     133     109     102
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1306    1340    1524 
   Size:       1306      34     184

  Fragments arranged by size: 

                1306     184      34
 
BfrBI ATG'CAT

Cuts at:      0     329    1524 
   Size:        329    1195
 
BglI GCCn_nnn'nGGC

Cuts at:      0      67    1524 
   Size:         67    1457
 
BglII A'GATC_T

Cuts at:      0     446    1524 
   Size:        446    1078
 
Bme1580I G_kGCm'C

Cuts at:      0    1312    1524 
   Size:       1312     212
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     447    1524 
   Size:        447    1077
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     169     252    1524 
   Size:        169      83    1272

  Fragments arranged by size: 

                1272     169      83
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274    1524 
   Size:        274    1250
 
Bpu10I CC'TnA_GC

Cuts at:      0    1159    1524 
   Size:       1159     365
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      45      65    1524 
   Size:         45      20    1459

  Fragments arranged by size: 

                1459      45      20
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      36    1361    1524 
   Size:         36    1325     163

  Fragments arranged by size: 

                1325     163      36
 
BsaBI GATnn'nnATC

Cuts at:      0     738    1524 
   Size:        738     786
 
BsaWI w'CCGG_w

Cuts at:      0     345     969    1001    1524 
   Size:        345     624      32     523

  Fragments arranged by size: 

                 624     523     345      32
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     284     314    1524 
   Size:        284      30    1210

  Fragments arranged by size: 

                1210     284      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      63      68     292     691    1524 
   Size:         63       5     224     399     833

  Fragments arranged by size: 

                 833     399     224      63       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     200     302    1524 
   Size:        200     102    1222

  Fragments arranged by size: 

                1222     200     102
 
BsmI GAATG_Cn'

Cuts at:      0     331     866    1524 
   Size:        331     535     658

  Fragments arranged by size: 

                 658     535     331
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     200     302    1312    1524 
   Size:         31     169     102    1010     212

  Fragments arranged by size: 

                1010     212     169     102      31
 
BspEI T'CCGG_A

Cuts at:      0     969    1001    1524 
   Size:        969      32     523

  Fragments arranged by size: 

                 969     523      32
 
BsrBI CCG'CTC

Cuts at:      0    1395    1524 
   Size:       1395     129
 
BsrFI r'CCGG_y

Cuts at:      0     345    1524 
   Size:        345    1179
 
BsrGI T'GTAC_A

Cuts at:      0     438    1524 
   Size:        438    1086
 
BssSI C'ACGA_G

Cuts at:      0      11    1524 
   Size:         11    1513
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1293    1524 
   Size:       1293     231
 
BstYI r'GATC_y

Cuts at:      0       3     446    1524 
   Size:          3     443    1078

  Fragments arranged by size: 

                1078     443       3
 
BtgI C'CryG_G

Cuts at:      0    1507    1524 
   Size:       1507      17
 
DraI TTT'AAA

Cuts at:      0     482    1524 
   Size:        482    1042
 
EarI CTCTTCn'nnn_

Cuts at:      0      34     378    1064    1524 
   Size:         34     344     686     460

  Fragments arranged by size: 

                 686     460     344      34
 
EcoICRI GAG'CTC

Cuts at:      0     198     300    1524 
   Size:        198     102    1224

  Fragments arranged by size: 

                1224     198     102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     725     799    1524 
   Size:        274     451      74     725

  Fragments arranged by size: 

                 725     451     274      74
 
EcoO109I rG'GnC_Cy

Cuts at:      0     412    1524 
   Size:        412    1112
 
EcoRI G'AATT_C

Cuts at:      0     638    1524 
   Size:        638     886
 
EcoRV GAT'ATC

Cuts at:      0     736    1524 
   Size:        736     788
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     494    1093    1524 
   Size:        494     599     431

  Fragments arranged by size: 

                 599     494     431
 
FspI TGC'GCA

Cuts at:      0    1481    1524 
   Size:       1481      43
 
HaeII r_GCGC'y

Cuts at:      0    1380    1524 
   Size:       1380     144
 
HincII GTy'rAC

Cuts at:      0    1145    1524 
   Size:       1145     379
 
MfeI C'AATT_G

Cuts at:      0     431    1524 
   Size:        431    1093
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     815    1524 
   Size:        815     709
 
MslI CAynn'nnrTG

Cuts at:      0     619    1291    1524 
   Size:        619     672     233

  Fragments arranged by size: 

                 672     619     233
 
MspA1I CmG'CkG

Cuts at:      0     902    1259    1524 
   Size:        902     357     265

  Fragments arranged by size: 

                 902     357     265
 
NsiI A_TGCA'T

Cuts at:      0     331    1524 
   Size:        331    1193
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     781     813    1524 
   Size:        781      32     711

  Fragments arranged by size: 

                 781     711      32
 
PvuII CAG'CTG

Cuts at:      0     902    1259    1524 
   Size:        902     357     265

  Fragments arranged by size: 

                 902     357     265
 
SacI G_AGCT'C

Cuts at:      0     200     302    1524 
   Size:        200     102    1222

  Fragments arranged by size: 

                1222     200     102
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1064    1524 
   Size:       1064     460
 
SmlI C'TyrA_G

Cuts at:      0      24      80    1524 
   Size:         24      56    1444

  Fragments arranged by size: 

                1444      56      24
 
StyI C'CwwG_G

Cuts at:      0     656    1524 
   Size:        656     868
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     229    1524 
   Size:        229    1295
 
TatI w'GTAC_w

Cuts at:      0     276     438    1524 
   Size:        276     162    1086

  Fragments arranged by size: 

                1086     276     162
 
Tth111I GACn'n_nGTC

Cuts at:      0     895    1524 
   Size:        895     629
 
XmnI GAAnn'nnTTC

Cuts at:      0     178     638     796    1524 
   Size:        178     460     158     728

  Fragments arranged by size: 

                 728     460     178     158
 

 Enzymes that do cut:

     AcuI     AgeI     AlfI    AlwNI     ApoI     AvaI    BamHI     BanI
    BanII     BbsI     BcgI    BfrBI     BglI    BglII Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI
    BseRI  BsiHKAI     BsmI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI
    BssSI    BstXI    BstYI     BtgI     DraI     EarI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII   HincII     MfeI
     MmeI     MslI   MspA1I     NsiI     PpiI    PvuII     SacI     SapI
     SmlI     StyI    TaqII     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI    BbvCI    BciVI     BclI     BlpI    BmgBI
     BmtI    BsaAI    BsaHI    BseYI     BsgI    BsiEI    BsiWI    BsmBI
    BspHI    BspMI    BsrDI   BssHII   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI     FseI    FspAI    Hin4I  HindIII     HpaI
     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI     XbaI     XcmI     XhoI     XmaI     ZraI
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