################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspI AviII BamHI BbrPI BclI BfrI BglII BsaBI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EarI Eco47III Eco81I EcoRI FbaI FseI FspI HincII HindII HpaI KpnI Ksp632I KspI MamI MfeI MluI MroI MseI MunI NcoI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 1448 CGCG CG^CG AhdI 1498 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 1312 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1498 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 112 CTCGGG C^[CT]CG[GA]G AvrII 711 CCTAGG C^CTAGG BalI 434 TGGCCA TGG^CCA BcgI 104 GCAGGGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 665 GTATCC GTATCC(6/5) BcnI 525 CCGGG CC^[GC]GG BlnI 711 CCTAGG C^CTAGG BsaAI 533 TACGTG [CT]AC^GT[GA] BsaI 572 GGTCTC GGTCTC(1/5) BsaWI 1502 TCCGGT [AT]^CCGG[AT] BsiEI 81 CGGCCG CG[GA][CT]^CG BsoBI 112 CTCGGG C^[CT]CG[GA]G BstUI 1448 CGCG CG^CG BstXI 412 CCATCCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1796 AGATCC [GA]^GATC[CT] EagI 81 CGGCCG C^GGCCG Eam1105I 1498 GACCTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 81 CGGCCG C^GGCCG Eco52I 81 CGGCCG C^GGCCG EcoRV 2055 GATATC GAT^ATC EcoT22I 910 ATGCAT ATGCA^T HindIII 1330 AAGCTT A^AGCTT MaeII 534 ACGT A^CGT MflI 1796 AGATCC [GA]^GATC[CT] MluNI 434 TGGCCA TGG^CCA MscI 434 TGGCCA TGG^CCA MvnI 1448 CGCG CG^CG NciI 525 CCGGG CC^[GC]GG NdeI 417 CATATG CA^TATG NheI 1570 GCTAGC G^CTAGC NsiI 910 ATGCAT ATGCA^T NspI 1936 GCATGC [GA]CATG^[CT] PleI 640 GAGTC GAGTC(4/5) PshAI 592 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 1011 CCTGCAGG CCTGCA^GG SphI 1936 GCATGC GCATG^C Sse8387I 1011 CCTGCAGG CCTGCA^GG StuI 1523 AGGCCT AGG^CCT TaqI 1548 TCGA T^CGA TthHB8I 1548 TCGA T^CGA XhoII 1796 AGATCC [GA]^GATC[CT] XmnI 1312 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 67 GGCGCC G[GA]^CG[CT]C 73 GGCGCC AfaI 1218 GTAC GT^AC 1294 GTAC ApaI 779 GGGCCC GGGCC^C 1913 GGGCCC BbeI 67 GGCGCC GGCGC^C 73 GGCGCC BbsI 1968 GAAGAC GAAGAC(2/6) 1471 GTCTTC BbvCI 732 GCTGAGG CCTCAGC(-5/-2) 1350 GCTGAGG BglI 83 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1878 GCCACTGTGGC BlpI 483 GCTGAGC GC^T[ACGT]AGC 1263 GCTCAGC Bpu1102I 483 GCTGAGC GC^T[ACGT]AGC 1263 GCTCAGC BpuAI 1968 GAAGAC GAAGAC(2/6) 1471 GTCTTC BsaHI 67 GGCGCC G[GA]^CG[CT]C 73 GGCGCC BspHI 200 TCATGA T^CATGA 498 TCATGA BsrBI 1642 CCGCTC CCGCTC(-3/-3) 181 GAGCGG BstAPI 793 GCAGGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1887 GCACCTCTTGC BstEII 652 GGTTACC G^GT[ACGT]ACC 1950 GGTGACC BstPI 652 GGTTACC G^GT[ACGT]ACC 1950 GGTGACC CelII 483 GCTGAGC GC^T[ACGT]AGC 1263 GCTCAGC DsaI 701 CCACGG C^C[GA][CT]GG 1667 CCGTGG EcoO65I 652 GGTTACC G^GT[ACGT]ACC 1950 GGTGACC FauI 16 GCGGG CCCGC(4/6) 183 GCGGG HgaI 1446 GACGC GACGC(5/10) 1202 GCGTC Hin1I 67 GGCGCC G[GA]^CG[CT]C 73 GGCGCC KasI 67 GGCGCC G^GCGCC 73 GGCGCC NaeI 70 GCCGGC GCC^GGC 76 GCCGGC NarI 67 GGCGCC GG^CGCC 73 GGCGCC NgoMIV 70 GCCGGC G^CCGGC 76 GCCGGC PflMI 770 CCATCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1199 CCAGCGTCTGG PstI 367 CTGCAG CTGCA^G 1012 CTGCAG RcaI 200 TCATGA T^CATGA 498 TCATGA RsaI 1218 GTAC GT^AC 1294 GTAC SacI 580 GAGCTC GAGCT^C 1150 GAGCTC SfoI 67 GGCGCC GGC^GCC 73 GGCGCC SgrAI 69 CGCCGGCG C[GA]^CCGG[CT]G 75 CGCCGGCG SmlI 1327 CTCAAG C^T[CT][GA]AG 1897 CTTGAG Van91I 770 CCATCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1199 CCAGCGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaII BanI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I TspRI XcmI