(Linear) (Six-base) MAPSORT of: KIBB5827.seq Check: 3599 from: 1 to: 2067 KIBB5827.orf With 182 enzymes: SgfI * September 25, 2012 15:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1667 2067 Size: 1667 400 AhdI GACnn_n'nnGTC Cuts at: 0 1503 2067 Size: 1503 564 AleI CACnn'nnGTG Cuts at: 0 1669 2067 Size: 1669 398 AlwNI CAG_nnn'CTG Cuts at: 0 1205 1271 1347 2067 Size: 1205 66 76 720 Fragments arranged by size: 1205 720 76 66 ApaI G_GGCC'C Cuts at: 0 783 1917 2067 Size: 783 1134 150 Fragments arranged by size: 1134 783 150 AvaI C'yCGr_G Cuts at: 0 112 2067 Size: 112 1955 AvrII C'CTAG_G Cuts at: 0 711 2067 Size: 711 1356 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1285 1318 2067 Size: 1285 33 749 Fragments arranged by size: 1285 749 33 BanI G'GyrC_C Cuts at: 0 67 73 1886 2067 Size: 67 6 1813 181 Fragments arranged by size: 1813 181 67 6 BanII G_rGCy'C Cuts at: 0 584 783 1154 1654 1701 1917 2067 Size: 584 199 371 500 47 216 150 Fragments arranged by size: 584 500 371 216 199 150 47 BbeI G_GCGC'C Cuts at: 0 71 77 2067 Size: 71 6 1990 Fragments arranged by size: 1990 71 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 1464 1975 2067 Size: 1464 511 92 Fragments arranged by size: 1464 511 92 BbvCI CC'TCA_GC Cuts at: 0 733 1351 2067 Size: 733 618 716 Fragments arranged by size: 733 716 618 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 93 127 2067 Size: 93 34 1940 Fragments arranged by size: 1940 93 34 BciVI GTATCCnnnnn_n' Cuts at: 0 676 2067 Size: 676 1391 BfrBI ATG'CAT Cuts at: 0 912 2067 Size: 912 1155 BglI GCCn_nnn'nGGC Cuts at: 0 89 1884 2067 Size: 89 1795 183 Fragments arranged by size: 1795 183 89 BlpI GC'TnA_GC Cuts at: 0 484 1264 2067 Size: 484 780 803 Fragments arranged by size: 803 780 484 Bme1580I G_kGCm'C Cuts at: 0 783 1917 2046 2067 Size: 783 1134 129 21 Fragments arranged by size: 1134 783 129 21 BmrI ACTGGGnnnn_n' Cuts at: 0 2049 2067 Size: 2049 18 BmtI G_CTAG'C Cuts at: 0 1574 2067 Size: 1574 493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 598 1227 1273 2067 Size: 352 246 629 46 794 Fragments arranged by size: 794 629 352 246 46 Bpu10I CC'TnA_GC Cuts at: 0 733 1156 1223 1351 2067 Size: 733 423 67 128 716 Fragments arranged by size: 733 716 423 128 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1312 1918 2067 Size: 1312 606 149 Fragments arranged by size: 1312 606 149 BsaI GGTCTCn'nnnn_ Cuts at: 0 578 2067 Size: 578 1489 BsaAI yAC'GTr Cuts at: 0 535 2067 Size: 535 1532 BsaHI Gr'CG_yC Cuts at: 0 68 74 2067 Size: 68 6 1993 Fragments arranged by size: 1993 68 6 BsaWI w'CCGG_w Cuts at: 0 1502 2067 Size: 1502 565 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1009 1039 2067 Size: 1009 30 1028 Fragments arranged by size: 1028 1009 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 101 281 455 1444 2067 Size: 101 180 174 989 623 Fragments arranged by size: 989 623 180 174 101 BseYI C'CCAG_C Cuts at: 0 831 852 1006 1626 1678 2060 2067 Size: 831 21 154 620 52 382 7 Fragments arranged by size: 831 620 382 154 52 21 7 BsiEI CG_ry'CG Cuts at: 0 84 2067 Size: 84 1983 BsiHKAI G_wGCw'C Cuts at: 0 584 1154 1259 1925 2067 Size: 584 570 105 666 142 Fragments arranged by size: 666 584 570 142 105 Bsp1286I G_dGCh'C Cuts at: 0 584 783 1154 1259 1654 1701 1917 1925 Size: 584 199 371 105 395 47 216 8 Cuts at: 1925 2046 2067 Size: 121 21 Fragments arranged by size: 584 395 371 216 199 121 105 47 21 8 BspHI T'CATG_A Cuts at: 0 200 498 2067 Size: 200 298 1569 Fragments arranged by size: 1569 298 200 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 213 374 784 1410 1575 1667 2067 Size: 213 161 410 626 165 92 400 Fragments arranged by size: 626 410 400 213 165 161 92 BsrBI CCG'CTC Cuts at: 0 183 1644 2067 Size: 183 1461 423 Fragments arranged by size: 1461 423 183 BsrFI r'CCGG_y Cuts at: 0 70 76 1538 2067 Size: 70 6 1462 529 Fragments arranged by size: 1462 529 70 6 BstAPI GCAn_nnn'nTGC Cuts at: 0 799 1893 2067 Size: 799 1094 174 Fragments arranged by size: 1094 799 174 BstEII G'GTnAC_C Cuts at: 0 652 1950 2067 Size: 652 1298 117 Fragments arranged by size: 1298 652 117 BstXI CCAn_nnnn'nTGG Cuts at: 0 419 2067 Size: 419 1648 BstYI r'GATC_y Cuts at: 0 1796 2067 Size: 1796 271 BtgI C'CryG_G Cuts at: 0 701 1667 2067 Size: 701 966 400 Fragments arranged by size: 966 701 400 BtsI GCAGTG_nn' Cuts at: 0 1636 1923 2067 Size: 1636 287 144 Fragments arranged by size: 1636 287 144 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 263 298 2067 Size: 263 35 1769 Fragments arranged by size: 1769 263 35 EaeI y'GGCC_r Cuts at: 0 81 434 1536 2067 Size: 81 353 1102 531 Fragments arranged by size: 1102 531 353 81 EagI C'GGCC_G Cuts at: 0 81 2067 Size: 81 1986 EcoICRI GAG'CTC Cuts at: 0 582 1152 2067 Size: 582 570 915 Fragments arranged by size: 915 582 570 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 598 1227 1273 2067 Size: 352 246 629 46 794 Fragments arranged by size: 794 629 352 246 46 EcoNI CCTnn'n_nnAGG Cuts at: 0 370 811 925 2067 Size: 370 441 114 1142 Fragments arranged by size: 1142 441 370 114 EcoO109I rG'GnC_Cy Cuts at: 0 225 429 737 780 1407 1429 1902 2067 Size: 225 204 308 43 627 22 473 165 Fragments arranged by size: 627 473 308 225 204 165 43 22 EcoRV GAT'ATC Cuts at: 0 2057 2067 Size: 2057 10 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 975 2067 Size: 975 1092 HaeII r_GCGC'y Cuts at: 0 71 77 112 2067 Size: 71 6 35 1955 Fragments arranged by size: 1955 71 35 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1489 1521 2067 Size: 1489 32 546 Fragments arranged by size: 1489 546 32 HindIII A'AGCT_T Cuts at: 0 1330 2067 Size: 1330 737 KasI G'GCGC_C Cuts at: 0 67 73 2067 Size: 67 6 1994 Fragments arranged by size: 1994 67 6 MscI TGG'CCA Cuts at: 0 436 2067 Size: 436 1631 MslI CAynn'nnrTG Cuts at: 0 417 497 772 1669 1706 1865 1928 2023 Size: 417 80 275 897 37 159 63 95 Cuts at: 2023 2067 Size: 44 Fragments arranged by size: 897 417 275 159 95 80 63 44 37 MspA1I CmG'CkG Cuts at: 0 1023 1215 1622 1689 2067 Size: 1023 192 407 67 378 Fragments arranged by size: 1023 407 378 192 67 NaeI GCC'GGC Cuts at: 0 72 78 2067 Size: 72 6 1989 Fragments arranged by size: 1989 72 6 NarI GG'CG_CC Cuts at: 0 68 74 2067 Size: 68 6 1993 Fragments arranged by size: 1993 68 6 NdeI CA'TA_TG Cuts at: 0 418 2067 Size: 418 1649 NgoMIV G'CCGG_C Cuts at: 0 70 76 2067 Size: 70 6 1991 Fragments arranged by size: 1991 70 6 NheI G'CTAG_C Cuts at: 0 1570 2067 Size: 1570 497 NsiI A_TGCA'T Cuts at: 0 914 2067 Size: 914 1153 NspI r_CATG'y Cuts at: 0 1940 2067 Size: 1940 127 PasI CC'CwG_GG Cuts at: 0 783 2067 Size: 783 1284 PflMI CCAn_nnn'nTGG Cuts at: 0 776 1205 2067 Size: 776 429 862 Fragments arranged by size: 862 776 429 PfoI T'CCnGG_A Cuts at: 0 722 2067 Size: 722 1345 PpuMI rG'GwC_Cy Cuts at: 0 225 429 737 1429 2067 Size: 225 204 308 692 638 Fragments arranged by size: 692 638 308 225 204 PshAI GACnn'nnGTC Cuts at: 0 596 2067 Size: 596 1471 PspOMI G'GGCC_C Cuts at: 0 779 1913 2067 Size: 779 1134 154 Fragments arranged by size: 1134 779 154 PstI C_TGCA'G Cuts at: 0 371 1016 2067 Size: 371 645 1051 Fragments arranged by size: 1051 645 371 PvuII CAG'CTG Cuts at: 0 1023 1215 1622 1689 2067 Size: 1023 192 407 67 378 Fragments arranged by size: 1023 407 378 192 67 SacI G_AGCT'C Cuts at: 0 584 1154 2067 Size: 584 570 913 Fragments arranged by size: 913 584 570 SbfI CC_TGCA'GG Cuts at: 0 1016 2067 Size: 1016 1051 SfcI C'TryA_G Cuts at: 0 367 510 1012 1478 1602 1617 1792 2011 Size: 367 143 502 466 124 15 175 219 Cuts at: 2011 2067 Size: 56 Fragments arranged by size: 502 466 367 219 175 143 124 56 15 SfoI GGC'GCC Cuts at: 0 69 75 2067 Size: 69 6 1992 Fragments arranged by size: 1992 69 6 SgrAI Cr'CCGG_yG Cuts at: 0 70 76 2067 Size: 70 6 1991 Fragments arranged by size: 1991 70 6 SmlI C'TyrA_G Cuts at: 0 1327 1897 2067 Size: 1327 570 170 Fragments arranged by size: 1327 570 170 SphI G_CATG'C Cuts at: 0 1940 2067 Size: 1940 127 StuI AGG'CCT Cuts at: 0 1525 2067 Size: 1525 542 StyI C'CwwG_G Cuts at: 0 711 926 950 2067 Size: 711 215 24 1117 Fragments arranged by size: 1117 711 215 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1020 2067 Size: 1020 1047 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 809 841 1009 1041 2067 Size: 809 32 168 32 1026 Fragments arranged by size: 1026 809 168 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 888 1248 1686 2067 Size: 888 360 438 381 Fragments arranged by size: 888 438 381 360 XmnI GAAnn'nnTTC Cuts at: 0 1316 2067 Size: 1316 751 Enzymes that do cut: AarI AhdI AleI AlwNI ApaI AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HindIII KasI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NsiI NspI PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI SbfI SfcI SfoI SgrAI SmlI SphI StuI StyI TaqII TstI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AlfI AloI ApaLI ApoI AscI AseI AsiSI BamHI BclI BglII BmgBI BplI BsaBI BsgI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EarI EciI EcoRI FseI FspI FspAI HincII HpaI KpnI MfeI MluI MmeI NcoI NotI NruI PacI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI ZraI