(Circular) (Six-base) MAPSORT of: pF1KE2554.seq Check: 48 from: 1 to: 4196 pF1KE2554 4196 bp With 182 enzymes: SgfI * December 15, 2014 12:34 .. AccI GT'mk_AC Cuts at: 1234 1234 Size: 4196 Acc65I G'GTAC_C Cuts at: 1212 1212 Size: 4196 AclI AA'CG_TT Cuts at: 4036 4036 Size: 4196 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 652 900 1062 2021 2453 3200 3468 652 Size: 248 162 959 432 747 268 1380 Fragments arranged by size: 1380 959 747 432 268 248 162 AflIII A'CryG_T Cuts at: 1548 2652 3629 1548 Size: 1104 977 2115 Fragments arranged by size: 2115 1104 977 AgeI A'CCGG_T Cuts at: 2584 2584 Size: 4196 AleI CACnn'nnGTG Cuts at: 365 865 365 Size: 500 3696 AlwNI CAG_nnn'CTG Cuts at: 1501 3068 1501 Size: 1567 2629 ApaLI G'TGCA_C Cuts at: 835 858 964 2966 835 Size: 23 106 2002 2065 Fragments arranged by size: 2065 2002 106 23 ApoI r'AATT_y Cuts at: 1105 1200 3549 3805 1105 Size: 95 2349 256 1496 Fragments arranged by size: 2349 1496 256 95 AseI AT'TA_AT Cuts at: 20 20 Size: 4196 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4196 AvaI C'yCGr_G Cuts at: 1216 1216 Size: 4196 BamHI G'GATC_C Cuts at: 1221 1221 Size: 4196 BanI G'GyrC_C Cuts at: 732 1212 1626 1874 1909 732 Size: 480 414 248 35 3019 Fragments arranged by size: 3019 480 414 248 35 BanII G_rGCy'C Cuts at: 186 194 253 887 1210 2240 186 Size: 8 59 634 323 1030 2142 Fragments arranged by size: 2142 1030 634 323 59 8 BbeI G_GCGC'C Cuts at: 736 1630 1878 736 Size: 894 248 3054 Fragments arranged by size: 3054 894 248 BbsI GAAGACnn'nnnn_ Cuts at: 834 834 Size: 4196 BciVI GTATCCnnnnn_n' Cuts at: 2094 2855 4196 2094 Size: 761 1341 2094 Fragments arranged by size: 2094 1341 761 BclI T'GATC_A Cuts at: 1716 1716 Size: 4196 BfrBI ATG'CAT Cuts at: 376 376 Size: 4196 BglI GCCn_nnn'nGGC Cuts at: 988 988 Size: 4196 BglII A'GATC_T Cuts at: 1711 3748 1711 Size: 2037 2159 BlpI GC'TnA_GC Cuts at: 1308 1308 Size: 4196 Bme1580I G_kGCm'C Cuts at: 839 862 968 1821 1914 2970 839 Size: 23 106 853 93 1056 2065 Fragments arranged by size: 2065 1056 853 106 93 23 BmgBI CAC'GTC Cuts at: 840 840 Size: 4196 BmrI ACTGGGnnnn_n' Cuts at: 523 1408 1489 1824 3968 523 Size: 885 81 335 2144 751 Fragments arranged by size: 2144 885 751 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2563 2563 Size: 4196 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 445 445 Size: 4196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 937 1372 2251 2743 3041 937 Size: 435 879 492 298 2092 Fragments arranged by size: 2092 879 492 435 298 BsaI GGTCTCn'nnnn_ Cuts at: 865 3900 865 Size: 3035 1161 BsaAI yAC'GTr Cuts at: 1549 1549 Size: 4196 BsaBI GATnn'nnATC Cuts at: 6 1710 3747 6 Size: 1704 2037 455 Fragments arranged by size: 2037 1704 455 BsaHI Gr'CG_yC Cuts at: 733 1627 1875 2577 4073 733 Size: 894 248 702 1496 856 Fragments arranged by size: 1496 894 856 702 248 BsaWI w'CCGG_w Cuts at: 1385 1906 2584 2858 3005 3416 1385 Size: 521 678 274 147 411 2165 Fragments arranged by size: 2165 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1175 1205 1175 Size: 30 4166 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 163 166 243 163 Size: 3 77 4116 Fragments arranged by size: 4116 77 3 BseYI C'CCAG_C Cuts at: 910 1621 2379 2956 3452 910 Size: 711 758 577 496 1654 Fragments arranged by size: 1654 758 711 577 496 BsiEI CG_ry'CG Cuts at: 104 1738 1784 2992 104 Size: 1634 46 1208 1308 Fragments arranged by size: 1634 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 194 253 839 862 887 968 1210 1988 2178 Size: 59 586 23 25 81 242 778 190 Cuts at: 2178 2970 194 Size: 792 1420 Fragments arranged by size: 1420 792 778 586 242 190 81 59 25 23 BsiWI C'GTAC_G Cuts at: 406 406 Size: 4196 Bsp1286I G_dGCh'C Cuts at: 186 194 253 839 862 887 968 1210 1821 Size: 8 59 586 23 25 81 242 611 Cuts at: 1821 1914 1988 2178 2240 2970 186 Size: 93 74 190 62 730 1412 Fragments arranged by size: 1412 730 611 586 242 190 93 81 74 62 59 25 23 8 BspEI T'CCGG_A Cuts at: 3416 3416 Size: 4196 BspHI T'CATG_A Cuts at: 3372 3372 Size: 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1246 1460 1762 2143 1246 Size: 214 302 381 3299 Fragments arranged by size: 3299 381 302 214 BsrBI CCG'CTC Cuts at: 50 2488 2542 4026 4191 50 Size: 2438 54 1484 165 55 Fragments arranged by size: 2438 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1160 2108 1160 Size: 948 3248 BsrFI r'CCGG_y Cuts at: 712 2194 2375 2584 712 Size: 1482 181 209 2324 Fragments arranged by size: 2324 1482 209 181 BssSI C'ACGA_G Cuts at: 967 2467 2825 967 Size: 1500 358 2338 Fragments arranged by size: 2338 1500 358 BstAPI GCAn_nnn'nTGC Cuts at: 479 1573 479 Size: 1094 3102 BstBI TT'CG_AA Cuts at: 2557 2557 Size: 4196 BstEII G'GTnAC_C Cuts at: 942 942 Size: 4196 BstYI r'GATC_y Cuts at: 1 1221 1688 1711 2046 2292 3304 3390 3402 Size: 1220 467 23 335 246 1012 86 12 Cuts at: 3402 3748 1 Size: 346 449 Fragments arranged by size: 1220 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 134 134 Size: 4196 BtgI C'CryG_G Cuts at: 107 107 Size: 4196 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2146 2327 2490 2146 Size: 181 163 3852 Fragments arranged by size: 3852 181 163 BtsI GCAGTG_nn' Cuts at: 265 1038 1439 265 Size: 773 401 3022 Fragments arranged by size: 3022 773 401 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1014 1049 1014 Size: 35 4161 DraI TTT'AAA Cuts at: 1195 1195 Size: 4196 DrdI GACnn_nn'nnGTC Cuts at: 1902 2760 1902 Size: 858 3338 EaeI y'GGCC_r Cuts at: 623 1166 1781 1955 2346 2373 3718 623 Size: 543 615 174 391 27 1345 1101 Fragments arranged by size: 1345 1101 615 543 391 174 27 EagI C'GGCC_G Cuts at: 1781 1781 Size: 4196 EarI CTCTTCn'nnn_ Cuts at: 140 559 931 2219 2429 140 Size: 419 372 1288 210 1907 Fragments arranged by size: 1907 1288 419 372 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2404 2714 2860 4006 2404 Size: 310 146 1146 2594 Fragments arranged by size: 2594 1146 310 146 EcoICRI GAG'CTC Cuts at: 192 251 885 1208 192 Size: 59 634 323 3180 Fragments arranged by size: 3180 634 323 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 445 652 900 1062 2021 2453 3200 3468 445 Size: 207 248 162 959 432 747 268 1173 Fragments arranged by size: 1173 959 747 432 268 248 207 162 EcoO109I rG'GnC_Cy Cuts at: 299 1335 299 Size: 1036 3160 EcoRI G'AATT_C Cuts at: 1200 1200 Size: 4196 EcoRV GAT'ATC Cuts at: 1086 1086 Size: 4196 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 414 2215 414 Size: 1801 2395 FspI TGC'GCA Cuts at: 1060 1977 1060 Size: 917 3279 HaeII r_GCGC'y Cuts at: 736 1630 1878 2900 3879 736 Size: 894 248 1022 979 1053 Fragments arranged by size: 1053 1022 979 894 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 286 318 334 366 2038 2068 2070 2100 286 Size: 32 16 32 1672 30 2 30 2382 Fragments arranged by size: 2382 1672 32 32 30 30 16 2 HincII GTy'rAC Cuts at: 1002 1235 1002 Size: 233 3963 KasI G'GCGC_C Cuts at: 732 1626 1874 732 Size: 894 248 3054 Fragments arranged by size: 3054 894 248 KpnI G_GTAC'C Cuts at: 1216 1216 Size: 4196 MluI A'CGCG_T Cuts at: 3629 3629 Size: 4196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 526 2867 3051 526 Size: 2341 184 1671 Fragments arranged by size: 2341 1671 184 MscI TGG'CCA Cuts at: 1957 1957 Size: 4196 MslI CAynn'nnrTG Cuts at: 365 377 865 2185 2312 365 Size: 12 488 1320 127 2249 Fragments arranged by size: 2249 1320 488 127 12 MspA1I CmG'CkG Cuts at: 449 851 1307 1621 1981 2994 3239 449 Size: 402 456 314 360 1013 245 1406 Fragments arranged by size: 1406 1013 456 402 360 314 245 NaeI GCC'GGC Cuts at: 2377 2377 Size: 4196 NarI GG'CG_CC Cuts at: 733 1627 1875 733 Size: 894 248 3054 Fragments arranged by size: 3054 894 248 NcoI C'CATG_G Cuts at: 107 107 Size: 4196 NgoMIV G'CCGG_C Cuts at: 2375 2375 Size: 4196 NsiI A_TGCA'T Cuts at: 378 378 Size: 4196 NspI r_CATG'y Cuts at: 214 380 522 1249 1749 2656 214 Size: 166 142 727 500 907 1754 Fragments arranged by size: 1754 907 727 500 166 142 PciI A'CATG_T Cuts at: 2652 2652 Size: 4196 PfoI T'CCnGG_A Cuts at: 140 244 140 Size: 104 4092 PmeI GTTT'AAAC Cuts at: 1195 1195 Size: 4196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3335 3367 3335 Size: 32 4164 PstI C_TGCA'G Cuts at: 284 1243 1928 284 Size: 959 685 2552 Fragments arranged by size: 2552 959 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4196 PvuII CAG'CTG Cuts at: 449 1621 1981 449 Size: 1172 360 2664 Fragments arranged by size: 2664 1172 360 RsrII CG'GwC_CG Cuts at: 2391 2391 Size: 4196 SacI G_AGCT'C Cuts at: 194 253 887 1210 194 Size: 59 634 323 3180 Fragments arranged by size: 3180 634 323 59 SalI G'TCGA_C Cuts at: 1233 1233 Size: 4196 SapI GCTCTTCn'nnn_ Cuts at: 2219 2429 2219 Size: 210 3986 SbfI CC_TGCA'GG Cuts at: 1243 1243 Size: 4196 ScaI AGT'ACT Cuts at: 487 3713 487 Size: 3226 970 SexAI A'CCwGG_T Cuts at: 938 938 Size: 4196 SfcI C'TryA_G Cuts at: 33 280 1239 1924 2917 3108 33 Size: 247 959 685 993 191 1121 Fragments arranged by size: 1121 993 959 685 247 191 SfoI GGC'GCC Cuts at: 734 1628 1876 734 Size: 894 248 3054 Fragments arranged by size: 3054 894 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4196 SmaI CCC'GGG Cuts at: 1218 1218 Size: 4196 SmlI C'TyrA_G Cuts at: 952 1351 2266 2758 3020 952 Size: 399 915 492 262 2128 Fragments arranged by size: 2128 915 492 399 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4196 SphI G_CATG'C Cuts at: 214 1249 1749 214 Size: 1035 500 2661 Fragments arranged by size: 2661 1035 500 StuI AGG'CCT Cuts at: 637 637 Size: 4196 StyI C'CwwG_G Cuts at: 107 1330 1684 107 Size: 1223 354 2619 Fragments arranged by size: 2619 1223 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1779 1912 2371 2580 1779 Size: 133 459 209 3395 Fragments arranged by size: 3395 459 209 133 TatI w'GTAC_w Cuts at: 485 608 3711 485 Size: 123 3103 970 Fragments arranged by size: 3103 970 123 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3713 3745 3713 Size: 32 4164 Tth111I GACn'n_nGTC Cuts at: 1993 1993 Size: 4196 XbaI T'CTAG_A Cuts at: 1227 1227 Size: 4196 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1691 1691 Size: 4196 XmaI C'CCGG_G Cuts at: 1216 1216 Size: 4196 XmnI GAAnn'nnTTC Cuts at: 637 673 637 Size: 36 4160 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BmtI Bpu10I BsgI BsmI BsmBI BsrGI BssHII BstXI BstZ17I ClaI DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI