Restriction map for pF1KE5454
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BciVI       BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraII       
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FseI        FspI        HaeII       HapII       
HgaI        Hin1I       HindIII     HpaII       KasI        
KpnI        KspI        MaeI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspI        MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tth111I     
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII         992  CGCG                 CG^CG
AciI          396  CCGC                 CCGC(-3/-1)
AcsI          599  AAATTC               [GA]^AATT[CT]
AflIII         99  ACATGT               A^C[GA][CT]GT
ApoI          599  AAATTC               [GA]^AATT[CT]
AspHI         559  GAGCTC               G[AT]GC[AT]^C
AvaI         1054  CCCGAG               C^[CT]CG[GA]G
AvaII         777  GGACC                G^G[AT]CC
BanI          906  GGCACC               G^G[CT][GA]CC
BbsI           34  GTCTTC               GAAGAC(2/6)
BcgI         1034  CGATTCTTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          288  TGATCA               T^GATCA
BpmI          978  CTGGAG               CTGGAG(16/14)
BpuAI          34  GTCTTC               GAAGAC(2/6)
BsaI          541  GGTCTC               GGTCTC(1/5)
BseRI         867  CTCCTC               GAGGAG(10/8)
BsiHKAI       559  GAGCTC               G[AT]GC[AT]^C
BsmI          166  GCATTC               GAATGC(1/-1)
BsoBI        1054  CCCGAG               C^[CT]CG[GA]G
BspLU11I       99  ACATGT               A^CATGT
BspMI         612  ACCTGC               ACCTGC(4/8)
BstAPI       1009  GCAACCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         992  CGCG                 CG^CG
DraI          714  TTTAAA               TTT^AAA
DraIII        406  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          459  CTCTTC               CTCTTC(1/4)
FauI          395  CCCGC                CCCGC(4/6)
FbaI          288  TGATCA               T^GATCA
HincII        820  GTTAAC               GT[CT]^[GA]AC
HindII        820  GTTAAC               GT[CT]^[GA]AC
HpaI          820  GTTAAC               GTT^AAC
HphI          999  TCACC                GGTGA(8/7)
Ksp632I       459  CTCTTC               CTCTTC(1/4)
MslI          516  CACGGTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          992  CGCG                 CG^CG
MwoI         1009  GCAACCTCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI           99  ACATGT               [GA]CATG^[CT]
PflMI         693  CCATGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          593  GACTC                GAGTC(4/5)
PvuII         292  CAGCTG               CAG^CTG
SacI          559  GAGCTC               GAGCT^C
SmlI           86  CTCAAG               C^T[CT][GA]AG
SspI          840  AATATT               AAT^ATT
Tsp45I        514  GTCAC                ^GT[GC]AC
Van91I        693  CCATGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          476  CCATTTTAGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

BanII         559  GAGCTC               G[GA]GC[CT]^C
              799  GAGCCC              
BbvCI        1045  CCTCAGC              CCTCAGC(-5/-2)
              154  GCTGAGG             
BbvI          703  GCAGC                GCAGC(8/12)
              310  GCTGC               
BmyI          559  GAGCTC               G[AGT]GC[ACT]^C
              799  GAGCCC              
BsaJI         972  CCCAGG               C^C[ACGT][ACGT]GG
             1022  CCTGGG              
BsgI          352  GTGCAG               GTGCAG(16/14)
              490  GTGCAG              
Bsp1286I      559  GAGCTC               G[AGT]GC[ACT]^C
              799  GAGCCC              
BsrI          538  ACTGG                ACTGG(1/-1)
               31  CCAGT               
Cac8I         706  GCAGGC               GC[ACGT]^[ACGT]GC
              903  GCAGGC              
CfoI           42  GCGC                 GCG^C
              991  GCGC                
DpnII         289  GATC                 ^GATC
              995  GATC                
Fnu4HI        310  GCTGC                GC^[ACGT]GC
              703  GCAGC               
HaeIII        142  GGCC                 GG^CC
              323  GGCC                
HhaI           42  GCGC                 GCG^C
              991  GCGC                
HinP1I         42  GCGC                 G^CGC
              991  GCGC                
ItaI          310  GCTGC                GC^[ACGT]GC
              703  GCAGC               
MaeIII        514  GTCAC                ^GT[ACGT]AC
              609  GTTAC               
MboI          289  GATC                 ^GATC
              995  GATC                
MspA1I        292  CAGCTG               C[AC]G^C[GT]G
              396  CCGCTG              
NdeII         289  GATC                 ^GATC
              995  GATC                
NlaIV          91  GGAACC               GG[ACGT]^[ACGT]CC
              906  GGCACC              
Sau3AI        289  GATC                 ^GATC
              995  GATC                
SexAI         407  ACCAGGT              A^CC[AT]GGT
              485  ACCTGGT             
SfaNI         628  GATGC                GCATC(5/9)
              271  GCATC               
TaqI          591  TCGA                 T^CGA
              889  TCGA                
TseI          310  GCTGC                G^C[AT]GC
              703  GCAGC               
Tsp509I       189  AATT                 ^AATT
              600  AATT                
TthHB8I       591  TCGA                 T^CGA
              889  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        BsiYI       BslI        BsmAI       BsrDI       
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
EcoRII      FokI        HinfI       MboII       MnlI        
MseI        MvaI        NlaIII      Sau96I      ScrFI       
TfiI        Tru9I       TspRI       
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