Restriction map for pF1KE5778
 (Linear) (Six-base) MAPSORT of: KIEE5778.seq  Check: 4788  from: 1  to: 1875



KIEE5778.orf

 With 182 enzymes: SgfI * 

                        June 20, 2019 10:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      91    1194    1875 
   Size:         91    1103     681

  Fragments arranged by size: 

                1103     681      91
 
AatII G_ACGT'C

Cuts at:      0     431    1875 
   Size:        431    1444
 
AccI GT'mk_AC

Cuts at:      0     495     837    1331    1875 
   Size:        495     342     494     544

  Fragments arranged by size: 

                 544     495     494     342
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     520    1875 
   Size:         26     494    1355

  Fragments arranged by size: 

                1355     494      26
 
AfeI AGC'GCT

Cuts at:      0     324    1875 
   Size:        324    1551
 
AleI CACnn'nnGTG

Cuts at:      0    1244    1828    1875 
   Size:       1244     584      47

  Fragments arranged by size: 

                1244     584      47
 
AlwNI CAG_nnn'CTG

Cuts at:      0      49     957    1875 
   Size:         49     908     918

  Fragments arranged by size: 

                 918     908      49
 
ApaLI G'TGCA_C

Cuts at:      0     578     960    1875 
   Size:        578     382     915

  Fragments arranged by size: 

                 915     578     382
 
BamHI G'GATC_C

Cuts at:      0     204    1875 
   Size:        204    1671
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     105    1875 
   Size:        105    1770
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1478    1512    1777    1811    1875 
   Size:       1478      34     265      34      64

  Fragments arranged by size: 

                1478     265      64      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1096    1875 
   Size:       1096     779
 
BclI T'GATC_A

Cuts at:      0    1407    1875 
   Size:       1407     468
 
Bme1580I G_kGCm'C

Cuts at:      0     582     964    1875 
   Size:        582     382     911

  Fragments arranged by size: 

                 911     582     382
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     788     856    1875 
   Size:        788      68    1019

  Fragments arranged by size: 

                1019     788      68
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     836    1875 
   Size:        836    1039
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496    1579    1875 
   Size:        496    1083     296

  Fragments arranged by size: 

                1083     496     296
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383    1850    1875 
   Size:        383    1467      25

  Fragments arranged by size: 

                1467     383      25
 
BsaAI yAC'GTr

Cuts at:      0     449    1842    1875 
   Size:        449    1393      33

  Fragments arranged by size: 

                1393     449      33
 
BsaHI Gr'CG_yC

Cuts at:      0     428    1875 
   Size:        428    1447
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1103    1875 
   Size:       1103     772
 
BseYI C'CCAG_C

Cuts at:      0     469     999    1541    1781    1875 
   Size:        469     530     542     240      94

  Fragments arranged by size: 

                 542     530     469     240      94
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     132     672    1198    1875 
   Size:        132     540     526     677

  Fragments arranged by size: 

                 677     540     526     132
 
BsiHKAI G_wGCw'C

Cuts at:      0     399     582     964    1800    1875 
   Size:        399     183     382     836      75

  Fragments arranged by size: 

                 836     399     382     183      75
 
BsiWI C'GTAC_G

Cuts at:      0     449    1875 
   Size:        449    1426
 
BsmI GAATG_Cn'

Cuts at:      0     409    1875 
   Size:        409    1466
 
Bsp1286I G_dGCh'C

Cuts at:      0     399     582     964    1800    1875 
   Size:        399     183     382     836      75

  Fragments arranged by size: 

                 836     399     382     183      75
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      91    1194    1875 
   Size:         91    1103     681

  Fragments arranged by size: 

                1103     681      91
 
BsrBI CCG'CTC

Cuts at:      0    1288    1809    1875 
   Size:       1288     521      66

  Fragments arranged by size: 

                1288     521      66
 
BsrDI GCAATG_nn'

Cuts at:      0     310    1875 
   Size:        310    1565
 
BsrFI r'CCGG_y

Cuts at:      0    1681    1875 
   Size:       1681     194
 
BssSI C'ACGA_G

Cuts at:      0     940    1776    1875 
   Size:        940     836      99

  Fragments arranged by size: 

                 940     836      99
 
BstEII G'GTnAC_C

Cuts at:      0    1206    1875 
   Size:       1206     669
 
BstYI r'GATC_y

Cuts at:      0     204    1060    1875 
   Size:        204     856     815

  Fragments arranged by size: 

                 856     815     204
 
BtgI C'CryG_G

Cuts at:      0    1198    1875 
   Size:       1198     677
 
BtsI GCAGTG_nn'

Cuts at:      0     253     432    1875 
   Size:        253     179    1443

  Fragments arranged by size: 

                1443     253     179
 
DraI TTT'AAA

Cuts at:      0    1346    1875 
   Size:       1346     529
 
EaeI y'GGCC_r

Cuts at:      0     678    1195    1810    1875 
   Size:        678     517     615      65

  Fragments arranged by size: 

                 678     615     517      65
 
EarI CTCTTCn'nnn_

Cuts at:      0     178    1622    1633    1875 
   Size:        178    1444      11     242

  Fragments arranged by size: 

                1444     242     178      11
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     202    1875 
   Size:        202    1673
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     496     520    1579    1875 
   Size:         26     470      24    1059     296

  Fragments arranged by size: 

                1059     470     296      26      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1508    1875 
   Size:       1508     367
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1729    1875 
   Size:       1729     146
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1056    1629    1875 
   Size:       1056     573     246

  Fragments arranged by size: 

                1056     573     246
 
HaeII r_GCGC'y

Cuts at:      0     128     326    1875 
   Size:        128     198    1549

  Fragments arranged by size: 

                1549     198     128
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     155     187    1079    1111    1241    1273    1357    1389 
   Size:        155      32     892      32     130      32      84      32

Cuts at:   1389    1875 
   Size:        486

  Fragments arranged by size: 

                 892     486     155     130      84      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0    1045    1875 
   Size:       1045     830
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1090    1146    1875 
   Size:       1090      56     729

  Fragments arranged by size: 

                1090     729      56
 
MslI CAynn'nnrTG

Cuts at:      0     343     862    1244    1828    1875 
   Size:        343     519     382     584      47

  Fragments arranged by size: 

                 584     519     382     343      47
 
MspA1I CmG'CkG

Cuts at:      0     977    1875 
   Size:        977     898
 
NaeI GCC'GGC

Cuts at:      0    1683    1875 
   Size:       1683     192
 
NdeI CA'TA_TG

Cuts at:      0    1495    1875 
   Size:       1495     380
 
NgoMIV G'CCGG_C

Cuts at:      0    1681    1875 
   Size:       1681     194
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     215    1875 
   Size:        215    1660
 
PstI C_TGCA'G

Cuts at:      0     850    1875 
   Size:        850    1025
 
ScaI AGT'ACT

Cuts at:      0     133    1875 
   Size:        133    1742
 
SfcI C'TryA_G

Cuts at:      0     846    1332    1686    1875 
   Size:        846     486     354     189

  Fragments arranged by size: 

                 846     486     354     189
 
SmlI C'TyrA_G

Cuts at:      0     398    1865    1875 
   Size:        398    1467      10

  Fragments arranged by size: 

                1467     398      10
 
StuI AGG'CCT

Cuts at:      0     946    1875 
   Size:        946     929
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     173     523    1242    1533    1875 
   Size:        173     350     719     291     342

  Fragments arranged by size: 

                 719     350     342     291     173
 
TatI w'GTAC_w

Cuts at:      0     131     796    1875 
   Size:        131     665    1079

  Fragments arranged by size: 

                1079     665     131
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     681     713    1120    1152    1875 
   Size:        681      32     407      32     723

  Fragments arranged by size: 

                 723     681     407      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     649    1875 
   Size:        649    1226
 
ZraI GAC'GTC

Cuts at:      0     429    1875 
   Size:        429    1446
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI     AleI    AlwNI    ApaLI
    BamHI     BbsI     BcgI    BciVI     BclI Bme1580I     BmrI     BplI
     BpmI    BpuEI    BsaAI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstEII    BstYI     BtgI     BtsI     DraI     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I  HindIII     MmeI
     MslI   MspA1I     NaeI     NdeI   NgoMIV    PflMI     PstI     ScaI
     SfcI     SmlI     StuI    TaqII     TatI     TstI  Tth111I     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AlfI     AloI
     ApaI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI    BanII     BbeI    BbvCI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI   Bpu10I     BsaI    BsaBI    BsaWI    BsaXI    BsiEI
    BsmBI    BspEI    BspHI    BsrGI   BssHII   BstAPI    BstBI    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NarI     NcoI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     XbaI
     XcmI     XhoI     XmaI     XmnI
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