(Circular) (Six-base) MAPSORT of: pF1KB5854.seq Check: 6436 from: 1 to: 4577 pF1KB5854 4577 bp With 182 enzymes: SgfI * September 25, 2012 15:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 442 442 Size: 4577 AccI GT'mk_AC Cuts at: 1615 1615 Size: 4577 Acc65I G'GTAC_C Cuts at: 1593 1593 Size: 4577 AclI AA'CG_TT Cuts at: 4417 4417 Size: 4577 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2402 2834 3581 3849 2402 Size: 432 747 268 3130 Fragments arranged by size: 3130 747 432 268 AflIII A'CryG_T Cuts at: 1310 1929 3033 4010 1310 Size: 619 1104 977 1877 Fragments arranged by size: 1877 1104 977 619 AgeI A'CCGG_T Cuts at: 1043 2965 1043 Size: 1922 2655 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1361 1361 Size: 4577 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 163 195 163 Size: 32 4545 AlwNI CAG_nnn'CTG Cuts at: 636 858 1882 3449 636 Size: 222 1024 1567 1764 Fragments arranged by size: 1764 1567 1024 222 ApaI G_GGCC'C Cuts at: 614 1372 614 Size: 758 3819 ApaLI G'TGCA_C Cuts at: 1012 3347 1012 Size: 2335 2242 ApoI r'AATT_y Cuts at: 343 1581 3930 4186 343 Size: 1238 2349 256 734 Fragments arranged by size: 2349 1238 734 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4577 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4577 AvaI C'yCGr_G Cuts at: 166 421 1138 1597 166 Size: 255 717 459 3146 Fragments arranged by size: 3146 717 459 255 BamHI G'GATC_C Cuts at: 1602 1602 Size: 4577 BanI G'GyrC_C Cuts at: 113 1122 1251 1593 2007 2255 2290 113 Size: 1009 129 342 414 248 35 2400 Fragments arranged by size: 2400 1009 414 342 248 129 35 BanII G_rGCy'C Cuts at: 614 1372 1591 2621 614 Size: 758 219 1030 2570 Fragments arranged by size: 2570 1030 758 219 BbeI G_GCGC'C Cuts at: 117 1126 2011 2259 117 Size: 1009 885 248 2435 Fragments arranged by size: 2435 1009 885 248 BbsI GAAGACnn'nnnn_ Cuts at: 1183 1183 Size: 4577 BciVI GTATCCnnnnn_n' Cuts at: 2475 3236 4577 2475 Size: 761 1341 2475 Fragments arranged by size: 2475 1341 761 BclI T'GATC_A Cuts at: 2097 2097 Size: 4577 BglI GCCn_nnn'nGGC Cuts at: 148 148 Size: 4577 BglII A'GATC_T Cuts at: 2092 4129 2092 Size: 2037 2540 BlpI GC'TnA_GC Cuts at: 1689 1689 Size: 4577 Bme1580I G_kGCm'C Cuts at: 614 1016 1256 1372 1440 2202 2295 3351 614 Size: 402 240 116 68 762 93 1056 1840 Fragments arranged by size: 1840 1056 762 402 240 116 93 68 BmgBI CAC'GTC Cuts at: 1377 1377 Size: 4577 BmrI ACTGGGnnnn_n' Cuts at: 1158 1789 1870 2205 4349 1158 Size: 631 81 335 2144 1386 Fragments arranged by size: 2144 1386 631 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 309 2944 309 Size: 2635 1942 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1267 1267 Size: 4577 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1075 1222 1468 1753 2632 3124 3422 1075 Size: 147 246 285 879 492 298 2230 Fragments arranged by size: 2230 879 492 298 285 246 147 BsaI GGTCTCn'nnnn_ Cuts at: 280 373 1051 4281 280 Size: 93 678 3230 576 Fragments arranged by size: 3230 678 576 93 BsaAI yAC'GTr Cuts at: 1260 1930 1260 Size: 670 3907 BsaBI GATnn'nnATC Cuts at: 6 2091 4128 6 Size: 2085 2037 455 Fragments arranged by size: 2085 2037 455 BsaHI Gr'CG_yC Cuts at: 114 1123 2008 2256 2958 4454 114 Size: 1009 885 248 702 1496 237 Fragments arranged by size: 1496 1009 885 702 248 237 BsaWI w'CCGG_w Cuts at: 977 1043 1766 2287 2965 3239 3386 3797 977 Size: 66 723 521 678 274 147 411 1757 Fragments arranged by size: 1757 723 678 521 411 274 147 66 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1545 1575 1545 Size: 30 4547 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1009 1009 Size: 4577 BseYI C'CCAG_C Cuts at: 1421 2002 2760 3337 3833 1421 Size: 581 758 577 496 2165 Fragments arranged by size: 2165 758 581 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 106 420 106 Size: 314 4263 BsiEI CG_ry'CG Cuts at: 104 2119 2165 3373 104 Size: 2015 46 1208 1308 Fragments arranged by size: 2015 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 556 1016 1085 1591 2369 2559 3351 556 Size: 460 69 506 778 190 792 1782 Fragments arranged by size: 1782 792 778 506 460 190 69 BsmI GAATG_Cn' Cuts at: 599 832 599 Size: 233 4344 Bsp1286I G_dGCh'C Cuts at: 556 614 1016 1085 1256 1372 1440 1591 2202 Size: 58 402 69 171 116 68 151 611 Cuts at: 2202 2295 2369 2559 2621 3351 556 Size: 93 74 190 62 730 1782 Fragments arranged by size: 1782 730 611 402 190 171 151 116 93 74 69 68 62 58 BspEI T'CCGG_A Cuts at: 3797 3797 Size: 4577 BspHI T'CATG_A Cuts at: 3753 3753 Size: 4577 BspMI ACCTGCnnnn'nnnn_ Cuts at: 298 442 764 1627 1841 2143 2524 298 Size: 144 322 863 214 302 381 2351 Fragments arranged by size: 2351 863 381 322 302 214 144 BsrBI CCG'CTC Cuts at: 50 1193 2869 2923 4407 4572 50 Size: 1143 1676 54 1484 165 55 Fragments arranged by size: 1676 1484 1143 165 55 54 BsrDI GCAATG_nn' Cuts at: 2489 2489 Size: 4577 BsrFI r'CCGG_y Cuts at: 1043 1220 1321 2575 2756 2965 1043 Size: 177 101 1254 181 209 2655 Fragments arranged by size: 2655 1254 209 181 177 101 BssSI C'ACGA_G Cuts at: 222 529 1015 1210 2848 3206 222 Size: 307 486 195 1638 358 1593 Fragments arranged by size: 1638 1593 486 358 307 195 BstAPI GCAn_nnn'nTGC Cuts at: 596 858 1274 1954 596 Size: 262 416 680 3219 Fragments arranged by size: 3219 680 416 262 BstBI TT'CG_AA Cuts at: 455 2938 455 Size: 2483 2094 BstEII G'GTnAC_C Cuts at: 1470 1470 Size: 4577 BstXI CCAn_nnnn'nTGG Cuts at: 813 813 Size: 4577 BstYI r'GATC_y Cuts at: 1 1457 1602 2069 2092 2427 2673 3685 3771 Size: 1456 145 467 23 335 246 1012 86 Cuts at: 3771 3783 4129 1 Size: 12 346 449 Fragments arranged by size: 1456 1012 467 449 346 335 246 145 86 23 12 BtgI C'CryG_G Cuts at: 107 564 107 Size: 457 4120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1059 2527 2708 2871 1059 Size: 1468 181 163 2765 Fragments arranged by size: 2765 1468 181 163 BtsI GCAGTG_nn' Cuts at: 118 1820 118 Size: 1702 2875 DraI TTT'AAA Cuts at: 1576 1576 Size: 4577 DraIII CAC_nnn'GTG Cuts at: 1020 1020 Size: 4577 DrdI GACnn_nn'nnGTC Cuts at: 2283 3141 2283 Size: 858 3719 EaeI y'GGCC_r Cuts at: 2162 2336 2727 2754 4099 2162 Size: 174 391 27 1345 2640 Fragments arranged by size: 2640 1345 391 174 27 EagI C'GGCC_G Cuts at: 2162 2162 Size: 4577 EarI CTCTTCn'nnn_ Cuts at: 239 278 296 643 2600 2810 239 Size: 39 18 347 1957 210 2006 Fragments arranged by size: 2006 1957 347 210 39 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2785 3095 3241 4387 2785 Size: 310 146 1146 2975 Fragments arranged by size: 2975 1146 310 146 EcoICRI GAG'CTC Cuts at: 1589 1589 Size: 4577 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1267 2402 2834 3581 3849 1267 Size: 1135 432 747 268 1995 Fragments arranged by size: 1995 1135 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 610 611 687 855 1368 1716 610 Size: 1 76 168 513 348 3471 Fragments arranged by size: 3471 513 348 168 76 1 EcoRI G'AATT_C Cuts at: 343 1581 343 Size: 1238 3339 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2596 2596 Size: 4577 FspI TGC'GCA Cuts at: 2358 2358 Size: 4577 HaeII r_GCGC'y Cuts at: 117 1126 2011 2259 3281 4260 117 Size: 1009 885 248 1022 979 434 Fragments arranged by size: 1022 1009 979 885 434 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2419 2449 2451 2481 2419 Size: 30 2 30 4515 Fragments arranged by size: 4515 30 30 2 HincII GTy'rAC Cuts at: 1616 1616 Size: 4577 KasI G'GCGC_C Cuts at: 113 1122 2007 2255 113 Size: 1009 885 248 2435 Fragments arranged by size: 2435 1009 885 248 KpnI G_GTAC'C Cuts at: 1597 1597 Size: 4577 MluI A'CGCG_T Cuts at: 4010 4010 Size: 4577 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3248 3432 3248 Size: 184 4393 MscI TGG'CCA Cuts at: 2338 2338 Size: 4577 MslI CAynn'nnrTG Cuts at: 1078 1117 1165 2566 2693 1078 Size: 39 48 1401 127 2962 Fragments arranged by size: 2962 1401 127 48 39 MspA1I CmG'CkG Cuts at: 566 680 1274 1364 1421 1688 2002 2362 3375 Size: 114 594 90 57 267 314 360 1013 Cuts at: 3375 3620 566 Size: 245 1523 Fragments arranged by size: 1523 1013 594 360 314 267 245 114 90 57 NaeI GCC'GGC Cuts at: 1222 1323 2758 1222 Size: 101 1435 3041 Fragments arranged by size: 3041 1435 101 NarI GG'CG_CC Cuts at: 114 1123 2008 2256 114 Size: 1009 885 248 2435 Fragments arranged by size: 2435 1009 885 248 NcoI C'CATG_G Cuts at: 107 107 Size: 4577 NdeI CA'TA_TG Cuts at: 714 714 Size: 4577 NgoMIV G'CCGG_C Cuts at: 1220 1321 2756 1220 Size: 101 1435 3041 Fragments arranged by size: 3041 1435 101 NspI r_CATG'y Cuts at: 1314 1630 2130 3037 1314 Size: 316 500 907 2854 Fragments arranged by size: 2854 907 500 316 PciI A'CATG_T Cuts at: 1310 3033 1310 Size: 1723 2854 PmeI GTTT'AAAC Cuts at: 1576 1576 Size: 4577 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3716 3748 3716 Size: 32 4545 PspOMI G'GGCC_C Cuts at: 610 1368 610 Size: 758 3819 PstI C_TGCA'G Cuts at: 496 1624 2309 496 Size: 1128 685 2764 Fragments arranged by size: 2764 1128 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4577 PvuII CAG'CTG Cuts at: 1274 1421 2002 2362 1274 Size: 147 581 360 3489 Fragments arranged by size: 3489 581 360 147 RsrII CG'GwC_CG Cuts at: 2772 2772 Size: 4577 SacI G_AGCT'C Cuts at: 1591 1591 Size: 4577 SacII CC_GC'GG Cuts at: 567 567 Size: 4577 SalI G'TCGA_C Cuts at: 1614 1614 Size: 4577 SapI GCTCTTCn'nnn_ Cuts at: 2600 2810 2600 Size: 210 4367 SbfI CC_TGCA'GG Cuts at: 1624 1624 Size: 4577 ScaI AGT'ACT Cuts at: 4094 4094 Size: 4577 SfcI C'TryA_G Cuts at: 33 492 1620 2305 3298 3489 33 Size: 459 1128 685 993 191 1121 Fragments arranged by size: 1128 1121 993 685 459 191 SfoI GGC'GCC Cuts at: 115 1124 2009 2257 115 Size: 1009 885 248 2435 Fragments arranged by size: 2435 1009 885 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4577 SmaI CCC'GGG Cuts at: 1599 1599 Size: 4577 SmlI C'TyrA_G Cuts at: 1090 1237 1483 1732 2647 3139 3401 1090 Size: 147 246 249 915 492 262 2266 Fragments arranged by size: 2266 915 492 262 249 246 147 SpeI A'CTAG_T Cuts at: 67 67 Size: 4577 SphI G_CATG'C Cuts at: 1630 2130 1630 Size: 500 4077 StuI AGG'CCT Cuts at: 595 595 Size: 4577 StyI C'CwwG_G Cuts at: 107 1466 1523 1711 2065 107 Size: 1359 57 188 354 2619 Fragments arranged by size: 2619 1359 354 188 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1222 1318 2160 2293 2752 2961 1222 Size: 96 842 133 459 209 2838 Fragments arranged by size: 2838 842 459 209 133 96 TatI w'GTAC_w Cuts at: 4092 4092 Size: 4577 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 826 858 4094 4126 826 Size: 32 3236 32 1277 Fragments arranged by size: 3236 1277 32 32 Tth111I GACn'n_nGTC Cuts at: 2374 2374 Size: 4577 XbaI T'CTAG_A Cuts at: 1608 1608 Size: 4577 XcmI CCAnnnn_n'nnnnTGG Cuts at: 207 2072 207 Size: 1865 2712 XmaI C'CCGG_G Cuts at: 1597 1597 Size: 4577 XmnI GAAnn'nnTTC Cuts at: 343 748 1312 343 Size: 405 564 3608 Fragments arranged by size: 3608 564 405 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI