(Circular) (Six-base) MAPSORT of: pF1KE2456.seq Check: 1622 from: 1 to: 8240 pF1KE2456 8240 bp With 182 enzymes: SgfI * March 1, 2013 10:20 .. AccI GT'mk_AC Cuts at: 3684 3820 4501 5278 3684 Size: 136 681 777 6646 Fragments arranged by size: 6646 777 681 136 Acc65I G'GTAC_C Cuts at: 5256 5256 Size: 8240 AclI AA'CG_TT Cuts at: 8080 8080 Size: 8240 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 173 626 1525 2767 3047 3068 3644 4169 4244 Size: 453 899 1242 280 21 576 525 75 Cuts at: 4244 4337 4346 6065 6497 7244 7512 173 Size: 93 9 1719 432 747 268 901 Fragments arranged by size: 1719 1242 901 899 747 576 525 453 432 280 268 93 75 21 9 AflIII A'CryG_T Cuts at: 5592 6696 7673 5592 Size: 1104 977 6159 Fragments arranged by size: 6159 1104 977 AgeI A'CCGG_T Cuts at: 5036 6628 5036 Size: 1592 6648 AhdI GACnn_n'nnGTC Cuts at: 5037 5037 Size: 8240 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 4037 4037 Size: 8240 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 878 910 878 Size: 32 8208 AlwNI CAG_nnn'CTG Cuts at: 196 298 604 646 862 1318 2962 5545 7112 Size: 102 306 42 216 456 1644 2583 1567 Cuts at: 7112 196 Size: 1324 Fragments arranged by size: 2583 1644 1567 1324 456 306 216 102 42 ApaLI G'TGCA_C Cuts at: 7010 7010 Size: 8240 ApoI r'AATT_y Cuts at: 916 1751 1861 1889 2049 2422 3222 3305 3526 Size: 835 110 28 160 373 800 83 221 Cuts at: 3526 4567 4959 5244 7593 7849 916 Size: 1041 392 285 2349 256 1307 Fragments arranged by size: 2349 1307 1041 835 800 392 373 285 256 221 160 110 83 28 AseI AT'TA_AT Cuts at: 20 2497 2720 3769 20 Size: 2477 223 1049 4491 Fragments arranged by size: 4491 2477 1049 223 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 8240 AvaI C'yCGr_G Cuts at: 314 3464 3563 5260 314 Size: 3150 99 1697 3294 Fragments arranged by size: 3294 3150 1697 99 AvrII C'CTAG_G Cuts at: 5131 5131 Size: 8240 BamHI G'GATC_C Cuts at: 3942 5265 3942 Size: 1323 6917 BanI G'GyrC_C Cuts at: 5256 5670 5918 5953 5256 Size: 414 248 35 7543 Fragments arranged by size: 7543 414 248 35 BanII G_rGCy'C Cuts at: 1389 2066 2802 3068 5254 6284 1389 Size: 677 736 266 2186 1030 3345 Fragments arranged by size: 3345 2186 1030 736 677 266 BbeI G_GCGC'C Cuts at: 5674 5922 5674 Size: 248 7992 BbsI GAAGACnn'nnnn_ Cuts at: 2947 3270 3453 4348 2947 Size: 323 183 895 6839 Fragments arranged by size: 6839 895 323 183 BbvCI CC'TCA_GC Cuts at: 2317 2663 2317 Size: 346 7894 BciVI GTATCCnnnnn_n' Cuts at: 6138 6899 8240 6138 Size: 761 1341 6138 Fragments arranged by size: 6138 1341 761 BclI T'GATC_A Cuts at: 4641 4770 4932 4995 5760 4641 Size: 129 162 63 765 7121 Fragments arranged by size: 7121 765 162 129 63 BfrBI ATG'CAT Cuts at: 1415 1415 Size: 8240 BglII A'GATC_T Cuts at: 400 694 4053 4984 5152 5167 5755 7792 400 Size: 294 3359 931 168 15 588 2037 848 Fragments arranged by size: 3359 2037 931 848 588 294 168 15 BlpI GC'TnA_GC Cuts at: 5352 5352 Size: 8240 Bme1580I G_kGCm'C Cuts at: 5865 5958 7014 5865 Size: 93 1056 7091 Fragments arranged by size: 7091 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 2945 3153 3619 3873 5452 5533 5868 8012 2945 Size: 208 466 254 1579 81 335 2144 3173 Fragments arranged by size: 3173 2144 1579 466 335 254 208 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 501 6607 501 Size: 6106 2134 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1193 2726 4259 5148 5238 1193 Size: 1533 1533 889 90 4195 Fragments arranged by size: 4195 1533 1533 889 90 Bpu10I CC'TnA_GC Cuts at: 2317 2663 2317 Size: 346 7894 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 397 1105 2197 5416 6295 6787 7085 397 Size: 708 1092 3219 879 492 298 1552 Fragments arranged by size: 3219 1552 1092 879 708 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4466 7944 4466 Size: 3478 4762 BsaAI yAC'GTr Cuts at: 4668 5593 4668 Size: 925 7315 BsaBI GATnn'nnATC Cuts at: 6 276 576 2765 4871 5754 7791 6 Size: 270 300 2189 2106 883 2037 455 Fragments arranged by size: 2189 2106 2037 883 455 300 270 BsaHI Gr'CG_yC Cuts at: 5671 5919 6621 8117 5671 Size: 248 702 1496 5794 Fragments arranged by size: 5794 1496 702 248 BsaWI w'CCGG_w Cuts at: 268 5036 5205 5429 5950 6628 6902 7049 7460 Size: 4768 169 224 521 678 274 147 411 Cuts at: 7460 268 Size: 1048 Fragments arranged by size: 4768 1048 678 521 411 274 224 169 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 250 280 878 908 2022 2052 3360 3390 3605 Size: 30 598 30 1114 30 1308 30 215 Cuts at: 3605 3635 3716 3746 250 Size: 30 81 30 4744 Fragments arranged by size: 4744 1308 1114 598 215 81 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 772 2030 4113 4166 772 Size: 1258 2083 53 4846 Fragments arranged by size: 4846 2083 1258 53 BseYI C'CCAG_C Cuts at: 1373 3923 5665 6423 7000 7496 1373 Size: 2550 1742 758 577 496 2117 Fragments arranged by size: 2550 2117 1742 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1389 4043 1389 Size: 2654 5586 BsiEI CG_ry'CG Cuts at: 104 5782 5828 7036 104 Size: 5678 46 1208 1308 Fragments arranged by size: 5678 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1389 2066 5254 6032 6222 7014 1389 Size: 677 3188 778 190 792 2615 Fragments arranged by size: 3188 2615 792 778 677 190 BsmI GAATG_Cn' Cuts at: 1782 1909 3741 1782 Size: 127 1832 6281 Fragments arranged by size: 6281 1832 127 BsmBI CGTCTCn'nnnn_ Cuts at: 2026 2026 Size: 8240 Bsp1286I G_dGCh'C Cuts at: 1389 2066 2802 3068 5254 5865 5958 6032 6222 Size: 677 736 266 2186 611 93 74 190 Cuts at: 6222 6284 7014 1389 Size: 62 730 2615 Fragments arranged by size: 2615 2186 736 730 677 611 266 190 93 74 62 BspEI T'CCGG_A Cuts at: 268 7460 268 Size: 7192 1048 BspHI T'CATG_A Cuts at: 1680 1827 3111 7416 1680 Size: 147 1284 4305 2504 Fragments arranged by size: 4305 2504 1284 147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 5290 5504 5806 6187 5290 Size: 214 302 381 7343 Fragments arranged by size: 7343 381 302 214 BsrBI CCG'CTC Cuts at: 50 1722 6532 6586 8070 8235 50 Size: 1672 4810 54 1484 165 55 Fragments arranged by size: 4810 1672 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2388 4136 4531 6152 2388 Size: 1748 395 1621 4476 Fragments arranged by size: 4476 1748 1621 395 BsrFI r'CCGG_y Cuts at: 5036 6238 6419 6628 5036 Size: 1202 181 209 6648 Fragments arranged by size: 6648 1202 209 181 BssSI C'ACGA_G Cuts at: 6511 6869 6511 Size: 358 7882 BstAPI GCAn_nnn'nTGC Cuts at: 5617 5617 Size: 8240 BstBI TT'CG_AA Cuts at: 6601 6601 Size: 8240 BstEII G'GTnAC_C Cuts at: 1009 5135 1009 Size: 4126 4114 BstXI CCAn_nnnn'nTGG Cuts at: 2809 2809 Size: 8240 BstYI r'GATC_y Cuts at: 1 199 271 400 571 694 3397 3942 4053 Size: 198 72 129 171 123 2703 545 111 Cuts at: 4053 4246 4984 5152 5167 5265 5732 5755 6090 Size: 193 738 168 15 98 467 23 335 Cuts at: 6090 6336 7348 7434 7446 7792 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 2703 1012 738 545 467 449 346 335 246 198 193 171 168 129 123 111 98 86 72 23 15 12 BstZ17I GTA'TAC Cuts at: 3821 4502 3821 Size: 681 7559 Bsu36I CC'TnA_GG Cuts at: 905 2343 905 Size: 1438 6802 BtgI C'CryG_G Cuts at: 3451 3451 Size: 8240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 6190 6371 6534 6190 Size: 181 163 7896 Fragments arranged by size: 7896 181 163 BtsI GCAGTG_nn' Cuts at: 261 498 5130 5483 261 Size: 237 4632 353 3018 Fragments arranged by size: 4632 3018 353 237 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1081 1116 1081 Size: 35 8205 DraI TTT'AAA Cuts at: 1461 1574 2007 3014 4976 5239 1461 Size: 113 433 1007 1962 263 4462 Fragments arranged by size: 4462 1962 1007 433 263 113 DrdI GACnn_nn'nnGTC Cuts at: 5946 6804 5946 Size: 858 7382 EaeI y'GGCC_r Cuts at: 389 5825 5999 6390 6417 7762 389 Size: 5436 174 391 27 1345 867 Fragments arranged by size: 5436 1345 867 391 174 27 EagI C'GGCC_G Cuts at: 5825 5825 Size: 8240 EarI CTCTTCn'nnn_ Cuts at: 470 605 905 2228 2990 3091 4322 6263 6473 Size: 135 300 1323 762 101 1231 1941 210 Cuts at: 6473 470 Size: 2237 Fragments arranged by size: 2237 1941 1323 1231 762 300 210 135 101 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 6448 6758 6904 8050 6448 Size: 310 146 1146 6638 Fragments arranged by size: 6638 1146 310 146 EcoICRI GAG'CTC Cuts at: 1387 2064 5252 1387 Size: 677 3188 4375 Fragments arranged by size: 4375 3188 677 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 173 626 1193 1525 2726 2767 3047 3068 3644 Size: 453 567 332 1201 41 280 21 576 Cuts at: 3644 4169 4244 4259 4337 4346 5148 5238 6065 Size: 525 75 15 78 9 802 90 827 Cuts at: 6065 6497 7244 7512 173 Size: 432 747 268 901 Fragments arranged by size: 1201 901 827 802 747 576 567 525 453 432 332 280 268 90 78 75 41 21 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 1743 2167 4412 1743 Size: 424 2245 5571 Fragments arranged by size: 5571 2245 424 EcoO109I rG'GnC_Cy Cuts at: 1350 3127 5379 1350 Size: 1777 2252 4211 Fragments arranged by size: 4211 2252 1777 EcoRI G'AATT_C Cuts at: 2049 2422 5244 2049 Size: 373 2822 5045 Fragments arranged by size: 5045 2822 373 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2890 4090 6259 2890 Size: 1200 2169 4871 Fragments arranged by size: 4871 2169 1200 FspI TGC'GCA Cuts at: 6021 6021 Size: 8240 HaeII r_GCGC'y Cuts at: 5674 5922 6944 7923 5674 Size: 248 1022 979 5991 Fragments arranged by size: 5991 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 166 198 250 256 282 286 288 318 392 Size: 32 52 6 26 4 2 30 74 Cuts at: 392 424 496 528 586 618 1809 1827 1841 Size: 32 72 32 58 32 1191 18 14 Cuts at: 1841 1859 1993 2025 2053 2085 3400 3432 3447 Size: 18 134 32 28 32 1315 32 15 Cuts at: 3447 3479 4463 4495 4662 4694 4932 4939 4964 Size: 32 984 32 167 32 238 7 25 Cuts at: 4964 4971 5023 5055 5137 5169 6082 6112 6114 Size: 7 52 32 82 32 913 30 2 Cuts at: 6114 6144 166 Size: 30 2262 Fragments arranged by size: 2262 1315 1191 984 913 238 167 134 82 74 72 58 52 52 32 32 32 32 32 32 32 32 32 32 32 32 30 30 30 28 26 25 18 18 15 14 7 7 6 4 2 2 HincII GTy'rAC Cuts at: 1877 5279 1877 Size: 3402 4838 HpaI GTT'AAC Cuts at: 1877 1877 Size: 8240 KasI G'GCGC_C Cuts at: 5670 5918 5670 Size: 248 7992 KpnI G_GTAC'C Cuts at: 5260 5260 Size: 8240 MfeI C'AATT_G Cuts at: 3557 3557 Size: 8240 MluI A'CGCG_T Cuts at: 7673 7673 Size: 8240 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 146 928 3188 3730 5206 6911 7095 146 Size: 782 2260 542 1476 1705 184 1291 Fragments arranged by size: 2260 1705 1476 1291 782 542 184 MscI TGG'CCA Cuts at: 6001 6001 Size: 8240 MslI CAynn'nnrTG Cuts at: 122 542 2362 2657 3116 6229 6356 122 Size: 420 1820 295 459 3113 127 2006 Fragments arranged by size: 3113 2006 1820 459 420 295 127 MspA1I CmG'CkG Cuts at: 1318 1373 5351 5665 6025 7038 7283 1318 Size: 55 3978 314 360 1013 245 2275 Fragments arranged by size: 3978 2275 1013 360 314 245 55 NaeI GCC'GGC Cuts at: 6421 6421 Size: 8240 NarI GG'CG_CC Cuts at: 5671 5919 5671 Size: 248 7992 NgoMIV G'CCGG_C Cuts at: 6419 6419 Size: 8240 NsiI A_TGCA'T Cuts at: 1417 1417 Size: 8240 NspI r_CATG'y Cuts at: 1415 4606 5293 5793 6700 1415 Size: 3191 687 500 907 2955 Fragments arranged by size: 3191 2955 907 687 500 PacI TTA_AT'TAA Cuts at: 1067 1067 Size: 8240 PciI A'CATG_T Cuts at: 6696 6696 Size: 8240 PflMI CCAn_nnn'nTGG Cuts at: 3075 3075 Size: 8240 PfoI T'CCnGG_A Cuts at: 4819 4819 Size: 8240 PmeI GTTT'AAAC Cuts at: 5239 5239 Size: 8240 PmlI CAC'GTG Cuts at: 4668 4668 Size: 8240 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 878 910 7379 7411 878 Size: 32 6469 32 1707 Fragments arranged by size: 6469 1707 32 32 PpuMI rG'GwC_Cy Cuts at: 3127 3127 Size: 8240 PshAI GACnn'nnGTC Cuts at: 3953 3953 Size: 8240 PsiI TTA'TAA Cuts at: 1163 1229 1163 Size: 66 8174 PstI C_TGCA'G Cuts at: 5287 5972 5287 Size: 685 7555 PvuI CG_AT'CG Cuts at: 104 104 Size: 8240 PvuII CAG'CTG Cuts at: 1318 1373 5665 6025 1318 Size: 55 4292 360 3533 Fragments arranged by size: 4292 3533 360 55 RsrII CG'GwC_CG Cuts at: 6435 6435 Size: 8240 SacI G_AGCT'C Cuts at: 1389 2066 5254 1389 Size: 677 3188 4375 Fragments arranged by size: 4375 3188 677 SalI G'TCGA_C Cuts at: 5277 5277 Size: 8240 SapI GCTCTTCn'nnn_ Cuts at: 4322 6263 6473 4322 Size: 1941 210 6089 Fragments arranged by size: 6089 1941 210 SbfI CC_TGCA'GG Cuts at: 5287 5287 Size: 8240 ScaI AGT'ACT Cuts at: 2948 7757 2948 Size: 4809 3431 SexAI A'CCwGG_T Cuts at: 3123 3123 Size: 8240 SfcI C'TryA_G Cuts at: 33 2044 3137 5283 5968 6961 7152 33 Size: 2011 1093 2146 685 993 191 1121 Fragments arranged by size: 2146 2011 1121 1093 993 685 191 SfoI GGC'GCC Cuts at: 5672 5920 5672 Size: 248 7992 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 8240 SmaI CCC'GGG Cuts at: 5262 5262 Size: 8240 SmlI C'TyrA_G Cuts at: 412 1120 2176 3563 5395 6310 6802 7064 412 Size: 708 1056 1387 1832 915 492 262 1588 Fragments arranged by size: 1832 1588 1387 1056 915 708 492 262 SpeI A'CTAG_T Cuts at: 67 3744 67 Size: 3677 4563 SphI G_CATG'C Cuts at: 1415 4606 5293 5793 1415 Size: 3191 687 500 3862 Fragments arranged by size: 3862 3191 687 500 SspI AAT'ATT Cuts at: 1225 2281 2575 2832 3474 1225 Size: 1056 294 257 642 5991 Fragments arranged by size: 5991 1056 642 294 257 StuI AGG'CCT Cuts at: 2443 4374 4407 4418 2443 Size: 1931 33 11 6265 Fragments arranged by size: 6265 1931 33 11 StyI C'CwwG_G Cuts at: 1489 1635 1914 2231 2330 5131 5374 5728 1489 Size: 146 279 317 99 2801 243 354 4001 Fragments arranged by size: 4001 2801 354 317 279 243 146 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 4799 5823 5956 6415 6624 4799 Size: 1024 133 459 209 6415 Fragments arranged by size: 6415 1024 459 209 133 TatI w'GTAC_w Cuts at: 2946 3726 5200 7755 2946 Size: 780 1474 2555 3431 Fragments arranged by size: 3431 2555 1474 780 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2022 2054 3716 3748 5101 5133 7757 7789 2022 Size: 32 1662 32 1353 32 2624 32 2473 Fragments arranged by size: 2624 2473 1662 1353 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 6037 6037 Size: 8240 XbaI T'CTAG_A Cuts at: 2646 5271 2646 Size: 2625 5615 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5735 5735 Size: 8240 XhoI C'TCGA_G Cuts at: 3563 3563 Size: 8240 XmaI C'CCGG_G Cuts at: 5260 5260 Size: 8240 XmnI GAAnn'nnTTC Cuts at: 2870 3245 4304 4345 2870 Size: 375 1059 41 6765 Fragments arranged by size: 6765 1059 375 41 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AleI ApaI AscI BaeI BcgI BglI BmgBI BmtI BsiWI BsrGI BssHII ClaI DraIII EcoRV FseI FspAI HindIII NcoI NdeI NheI NotI NruI PasI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI ZraI