(Linear) (Six-base) MAPSORT of: KIEE2456.seq Check: 6217 from: 1 to: 5127 KIEE2456.orf With 182 enzymes: SgfI * March 1, 2013 10:20 .. AccI GT'mk_AC Cuts at: 0 3576 3712 4393 5127 Size: 3576 136 681 734 Fragments arranged by size: 3576 734 681 136 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 65 518 1417 2659 2939 2960 3536 4061 Size: 65 453 899 1242 280 21 576 525 Cuts at: 4061 4136 4229 4238 5127 Size: 75 93 9 889 Fragments arranged by size: 1242 899 889 576 525 453 280 93 75 65 21 9 AgeI A'CCGG_T Cuts at: 0 4928 5127 Size: 4928 199 AhdI GACnn_n'nnGTC Cuts at: 0 4929 5127 Size: 4929 198 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3929 5127 Size: 3929 1198 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 770 802 5127 Size: 770 32 4325 Fragments arranged by size: 4325 770 32 AlwNI CAG_nnn'CTG Cuts at: 0 88 190 496 538 754 1210 2854 5127 Size: 88 102 306 42 216 456 1644 2273 Fragments arranged by size: 2273 1644 456 306 216 102 88 42 ApoI r'AATT_y Cuts at: 0 808 1643 1753 1781 1941 2314 3114 3197 Size: 808 835 110 28 160 373 800 83 Cuts at: 3197 3418 4459 4851 5127 Size: 221 1041 392 276 Fragments arranged by size: 1041 835 808 800 392 373 276 221 160 110 83 28 AseI AT'TA_AT Cuts at: 0 2389 2612 3661 5127 Size: 2389 223 1049 1466 Fragments arranged by size: 2389 1466 1049 223 AvaI C'yCGr_G Cuts at: 0 206 3356 3455 5127 Size: 206 3150 99 1672 Fragments arranged by size: 3150 1672 206 99 AvrII C'CTAG_G Cuts at: 0 5023 5127 Size: 5023 104 BamHI G'GATC_C Cuts at: 0 3834 5127 Size: 3834 1293 BanII G_rGCy'C Cuts at: 0 1281 1958 2694 2960 5127 Size: 1281 677 736 266 2167 Fragments arranged by size: 2167 1281 736 677 266 BbsI GAAGACnn'nnnn_ Cuts at: 0 2839 3162 3345 4240 5127 Size: 2839 323 183 895 887 Fragments arranged by size: 2839 895 887 323 183 BbvCI CC'TCA_GC Cuts at: 0 2209 2555 5127 Size: 2209 346 2572 Fragments arranged by size: 2572 2209 346 BclI T'GATC_A Cuts at: 0 4533 4662 4824 4887 5127 Size: 4533 129 162 63 240 Fragments arranged by size: 4533 240 162 129 63 BfrBI ATG'CAT Cuts at: 0 1307 5127 Size: 1307 3820 BglII A'GATC_T Cuts at: 0 292 586 3945 4876 5044 5059 5127 Size: 292 294 3359 931 168 15 68 Fragments arranged by size: 3359 931 294 292 168 68 15 BmrI ACTGGGnnnn_n' Cuts at: 0 2837 3045 3511 3765 5127 Size: 2837 208 466 254 1362 Fragments arranged by size: 2837 1362 466 254 208 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 393 5127 Size: 393 4734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1085 2618 4151 5040 5127 Size: 1085 1533 1533 889 87 Fragments arranged by size: 1533 1533 1085 889 87 Bpu10I CC'TnA_GC Cuts at: 0 2209 2555 5127 Size: 2209 346 2572 Fragments arranged by size: 2572 2209 346 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 289 997 2089 5127 Size: 289 708 1092 3038 Fragments arranged by size: 3038 1092 708 289 BsaI GGTCTCn'nnnn_ Cuts at: 0 4358 5127 Size: 4358 769 BsaAI yAC'GTr Cuts at: 0 4560 5127 Size: 4560 567 BsaBI GATnn'nnATC Cuts at: 0 168 468 2657 4763 5127 Size: 168 300 2189 2106 364 Fragments arranged by size: 2189 2106 364 300 168 BsaWI w'CCGG_w Cuts at: 0 160 4928 5097 5127 Size: 160 4768 169 30 Fragments arranged by size: 4768 169 160 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 142 172 770 800 1914 1944 3252 3282 Size: 142 30 598 30 1114 30 1308 30 Cuts at: 3282 3497 3527 3608 3638 5127 Size: 215 30 81 30 1489 Fragments arranged by size: 1489 1308 1114 598 215 142 81 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 664 1922 4005 4058 5127 Size: 664 1258 2083 53 1069 Fragments arranged by size: 2083 1258 1069 664 53 BseYI C'CCAG_C Cuts at: 0 1265 3815 5127 Size: 1265 2550 1312 Fragments arranged by size: 2550 1312 1265 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1281 3935 5127 Size: 1281 2654 1192 Fragments arranged by size: 2654 1281 1192 BsiHKAI G_wGCw'C Cuts at: 0 1281 1958 5127 Size: 1281 677 3169 Fragments arranged by size: 3169 1281 677 BsmI GAATG_Cn' Cuts at: 0 1674 1801 3633 5127 Size: 1674 127 1832 1494 Fragments arranged by size: 1832 1674 1494 127 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1918 5127 Size: 1918 3209 Bsp1286I G_dGCh'C Cuts at: 0 1281 1958 2694 2960 5127 Size: 1281 677 736 266 2167 Fragments arranged by size: 2167 1281 736 677 266 BspEI T'CCGG_A Cuts at: 0 160 5127 Size: 160 4967 BspHI T'CATG_A Cuts at: 0 1572 1719 3003 5127 Size: 1572 147 1284 2124 Fragments arranged by size: 2124 1572 1284 147 BsrBI CCG'CTC Cuts at: 0 1614 5127 Size: 1614 3513 BsrDI GCAATG_nn' Cuts at: 0 2280 4028 4423 5127 Size: 2280 1748 395 704 Fragments arranged by size: 2280 1748 704 395 BsrFI r'CCGG_y Cuts at: 0 4928 5127 Size: 4928 199 BstEII G'GTnAC_C Cuts at: 0 901 5027 5127 Size: 901 4126 100 Fragments arranged by size: 4126 901 100 BstXI CCAn_nnnn'nTGG Cuts at: 0 2701 5127 Size: 2701 2426 BstYI r'GATC_y Cuts at: 0 91 163 292 463 586 3289 3834 3945 Size: 91 72 129 171 123 2703 545 111 Cuts at: 3945 4138 4876 5044 5059 5127 Size: 193 738 168 15 68 Fragments arranged by size: 2703 738 545 193 171 168 129 123 111 91 72 68 15 BstZ17I GTA'TAC Cuts at: 0 3713 4394 5127 Size: 3713 681 733 Fragments arranged by size: 3713 733 681 Bsu36I CC'TnA_GG Cuts at: 0 797 2235 5127 Size: 797 1438 2892 Fragments arranged by size: 2892 1438 797 BtgI C'CryG_G Cuts at: 0 3343 5127 Size: 3343 1784 BtsI GCAGTG_nn' Cuts at: 0 153 390 5022 5127 Size: 153 237 4632 105 Fragments arranged by size: 4632 237 153 105 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 973 1008 5127 Size: 973 35 4119 Fragments arranged by size: 4119 973 35 DraI TTT'AAA Cuts at: 0 1353 1466 1899 2906 4868 5127 Size: 1353 113 433 1007 1962 259 Fragments arranged by size: 1962 1353 1007 433 259 113 EaeI y'GGCC_r Cuts at: 0 281 5127 Size: 281 4846 EarI CTCTTCn'nnn_ Cuts at: 0 362 497 797 2120 2882 2983 4214 5127 Size: 362 135 300 1323 762 101 1231 913 Fragments arranged by size: 1323 1231 913 762 362 300 135 101 EcoICRI GAG'CTC Cuts at: 0 1279 1956 5127 Size: 1279 677 3171 Fragments arranged by size: 3171 1279 677 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 65 518 1085 1417 2618 2659 2939 2960 Size: 65 453 567 332 1201 41 280 21 Cuts at: 2960 3536 4061 4136 4151 4229 4238 5040 5127 Size: 576 525 75 15 78 9 802 87 Fragments arranged by size: 1201 802 576 567 525 453 332 280 87 78 75 65 41 21 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 1635 2059 4304 5127 Size: 1635 424 2245 823 Fragments arranged by size: 2245 1635 823 424 EcoO109I rG'GnC_Cy Cuts at: 0 1242 3019 5127 Size: 1242 1777 2108 Fragments arranged by size: 2108 1777 1242 EcoRI G'AATT_C Cuts at: 0 1941 2314 5127 Size: 1941 373 2813 Fragments arranged by size: 2813 1941 373 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2782 3982 5127 Size: 2782 1200 1145 Fragments arranged by size: 2782 1200 1145 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 58 90 142 148 174 178 180 210 Size: 58 32 52 6 26 4 2 30 Cuts at: 210 284 316 388 420 478 510 1701 1719 Size: 74 32 72 32 58 32 1191 18 Cuts at: 1719 1733 1751 1885 1917 1945 1977 3292 3324 Size: 14 18 134 32 28 32 1315 32 Cuts at: 3324 3339 3371 4355 4387 4554 4586 4824 4831 Size: 15 32 984 32 167 32 238 7 Cuts at: 4831 4856 4863 4915 4947 5029 5061 5127 Size: 25 7 52 32 82 32 66 Fragments arranged by size: 1315 1191 984 238 167 134 82 74 72 66 58 58 52 52 32 32 32 32 32 32 32 32 32 32 32 32 30 28 26 25 18 18 15 14 7 7 6 4 2 HincII GTy'rAC Cuts at: 0 1769 5127 Size: 1769 3358 HpaI GTT'AAC Cuts at: 0 1769 5127 Size: 1769 3358 MfeI C'AATT_G Cuts at: 0 3449 5127 Size: 3449 1678 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 38 820 3080 3622 5098 5127 Size: 38 782 2260 542 1476 29 Fragments arranged by size: 2260 1476 782 542 38 29 MslI CAynn'nnrTG Cuts at: 0 14 434 2254 2549 3008 5127 Size: 14 420 1820 295 459 2119 Fragments arranged by size: 2119 1820 459 420 295 14 MspA1I CmG'CkG Cuts at: 0 1210 1265 5127 Size: 1210 55 3862 Fragments arranged by size: 3862 1210 55 NsiI A_TGCA'T Cuts at: 0 1309 5127 Size: 1309 3818 NspI r_CATG'y Cuts at: 0 1307 4498 5127 Size: 1307 3191 629 Fragments arranged by size: 3191 1307 629 PacI TTA_AT'TAA Cuts at: 0 959 5127 Size: 959 4168 PflMI CCAn_nnn'nTGG Cuts at: 0 2967 5127 Size: 2967 2160 PfoI T'CCnGG_A Cuts at: 0 4711 5127 Size: 4711 416 PmlI CAC'GTG Cuts at: 0 4560 5127 Size: 4560 567 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 770 802 5127 Size: 770 32 4325 Fragments arranged by size: 4325 770 32 PpuMI rG'GwC_Cy Cuts at: 0 3019 5127 Size: 3019 2108 PshAI GACnn'nnGTC Cuts at: 0 3845 5127 Size: 3845 1282 PsiI TTA'TAA Cuts at: 0 1055 1121 5127 Size: 1055 66 4006 Fragments arranged by size: 4006 1055 66 PvuII CAG'CTG Cuts at: 0 1210 1265 5127 Size: 1210 55 3862 Fragments arranged by size: 3862 1210 55 SacI G_AGCT'C Cuts at: 0 1281 1958 5127 Size: 1281 677 3169 Fragments arranged by size: 3169 1281 677 SapI GCTCTTCn'nnn_ Cuts at: 0 4214 5127 Size: 4214 913 ScaI AGT'ACT Cuts at: 0 2840 5127 Size: 2840 2287 SexAI A'CCwGG_T Cuts at: 0 3015 5127 Size: 3015 2112 SfcI C'TryA_G Cuts at: 0 1936 3029 5127 Size: 1936 1093 2098 Fragments arranged by size: 2098 1936 1093 SmlI C'TyrA_G Cuts at: 0 304 1012 2068 3455 5127 Size: 304 708 1056 1387 1672 Fragments arranged by size: 1672 1387 1056 708 304 SpeI A'CTAG_T Cuts at: 0 3636 5127 Size: 3636 1491 SphI G_CATG'C Cuts at: 0 1307 4498 5127 Size: 1307 3191 629 Fragments arranged by size: 3191 1307 629 SspI AAT'ATT Cuts at: 0 1117 2173 2467 2724 3366 5127 Size: 1117 1056 294 257 642 1761 Fragments arranged by size: 1761 1117 1056 642 294 257 StuI AGG'CCT Cuts at: 0 2335 4266 4299 4310 5127 Size: 2335 1931 33 11 817 Fragments arranged by size: 2335 1931 817 33 11 StyI C'CwwG_G Cuts at: 0 1381 1527 1806 2123 2222 5023 5127 Size: 1381 146 279 317 99 2801 104 Fragments arranged by size: 2801 1381 317 279 146 104 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 4691 5127 Size: 4691 436 TatI w'GTAC_w Cuts at: 0 2838 3618 5092 5127 Size: 2838 780 1474 35 Fragments arranged by size: 2838 1474 780 35 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1914 1946 3608 3640 4993 5025 5127 Size: 1914 32 1662 32 1353 32 102 Fragments arranged by size: 1914 1662 1353 102 32 32 32 XbaI T'CTAG_A Cuts at: 0 2538 5127 Size: 2538 2589 XhoI C'TCGA_G Cuts at: 0 3455 5127 Size: 3455 1672 XmnI GAAnn'nnTTC Cuts at: 0 2762 3137 4196 4237 5127 Size: 2762 375 1059 41 890 Fragments arranged by size: 2762 1059 890 375 41 Enzymes that do cut: AccI AcuI AgeI AhdI AlfI AloI AlwNI ApoI AseI AvaI AvrII BamHI BanII BbsI BbvCI BclI BfrBI BglII BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HpaI MfeI MmeI MslI MspA1I NsiI NspI PacI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PvuII SacI SapI ScaI SexAI SfcI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI XbaI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AleI ApaI ApaLI AscI AsiSI BaeI BanI BbeI BcgI BciVI BglI BlpI Bme1580I BmgBI BmtI BsaHI BsiEI BsiWI BspMI BsrGI BssHII BssSI BstAPI BstBI BtgZI ClaI DraIII DrdI EagI EciI EcoRV FseI FspI FspAI HaeII HindIII KasI KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PasI PciI PmeI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI Tth111I XcmI XmaI ZraI