################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NarI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Alw44I 826 GTGCAC G^TGCAC AlwNI 452 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 342 CCTCAGG CC^T[ACGT]AGG ApaLI 826 GTGCAC G^TGCAC AspHI 826 GTGCAC G[AT]GC[AT]^C BanII 477 GGGCTC G[GA]GC[CT]^C BbsI 82 GAAGAC GAAGAC(2/6) BfaI 301 CTAG C^TAG BlpI 732 GCTGAGC GC^T[ACGT]AGC Bpu1102I 732 GCTGAGC GC^T[ACGT]AGC BpuAI 82 GAAGAC GAAGAC(2/6) BsaBI 667 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 601 GAGACC GGTCTC(1/5) BsiHKAI 826 GTGCAC G[AT]GC[AT]^C BsmBI 496 CGTCTC CGTCTC(1/5) BstAPI 378 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 794 CCATCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 282 GGATCT [GA]^GATC[CT] Bsu36I 342 CCTCAGG CC^T[ACGT]AGG CelII 732 GCTGAGC GC^T[ACGT]AGC DraII 941 AGGCCCT [GA]G^G[ACGT]CC[CT] EarI 499 CTCTTC CTCTTC(1/4) Eco81I 342 CCTCAGG CC^T[ACGT]AGG EcoO109I 941 AGGCCCT [GA]G^G[ACGT]CC[CT] Ksp632I 499 CTCTTC CTCTTC(1/4) MaeI 301 CTAG C^TAG MamI 667 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 282 GGATCT [GA]^GATC[CT] NaeI 835 GCCGGC GCC^GGC NcoI 908 CCATGG C^CATGG NgoMIV 835 GCCGGC G^CCGGC NheI 300 GCTAGC G^CTAGC PflMI 451 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 719 CAGCTG CAG^CTG StuI 161 AGGCCT AGG^CCT Van91I 451 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 282 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 237 GACGCC G[GA]^CG[CT]C 853 GACGCC AfaI 90 GTAC GT^AC 420 GTAC AlwI 192 GGATC GGATC(4/5) 282 GGATC AvaI 77 CCCGGG C^[CT]CG[GA]G 188 CCCGGG AvaII 45 GGTCC G^G[AT]CC 266 GGACC BanI 347 GGTGCC G^G[CT][GA]CC 377 GGCACC BglI 31 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 448 GCCCCAGTGGC BpmI 139 CTCCAG CTGGAG(16/14) 964 CTGGAG BsaHI 237 GACGCC G[GA]^CG[CT]C 853 GACGCC BsmFI 265 GGGAC GGGAC(10/14) 12 GTCCC BsoBI 77 CCCGGG C^[CT]CG[GA]G 188 CCCGGG BsrDI 831 CATTGC GCAATG(2/0) 580 GCAATG BsrFI 380 ACCGGC [GA]^CCGG[CT] 835 GCCGGC Cfr10I 380 ACCGGC [GA]^CCGG[CT] 835 GCCGGC EcoT14I 269 CCAAGG C^C[AT][AT]GG 908 CCATGG Hin1I 237 GACGCC G[GA]^CG[CT]C 853 GACGCC MaeII 473 ACGT A^CGT 991 ACGT MaeIII 470 GTGAC ^GT[ACGT]AC 818 GTGAC MseI 764 TTAA T^TAA 953 TTAA MslI 811 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 880 CACTACTATG RsaI 90 GTAC GT^AC 420 GTAC SfaNI 406 GATGC GCATC(5/9) 960 GATGC SmaI 77 CCCGGG CCC^GGG 188 CCCGGG SmlI 214 CTTGAG C^T[CT][GA]AG 901 CTCAAG StyI 269 CCAAGG C^C[AT][AT]GG 908 CCATGG TaqI 95 TCGA T^CGA 524 TCGA Tru9I 764 TTAA T^TAA 953 TTAA Tsp45I 470 GTGAC ^GT[GC]AC 818 GTGAC TthHB8I 95 TCGA T^CGA 524 TCGA XcmI 32 CCATGTTGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 779 CCACGGGCCTGTTGG XmaCI 77 CCCGGG C^CCGGG 188 CCCGGG XmaI 77 CCCGGG C^CCGGG 188 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI BalI BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TfiI TseI TspRI