(Circular) (Six-base) MAPSORT of: pF1KE2671.seq Check: 1906 from: 1 to: 4112 pF1KE2671 4112 bp With 182 enzymes: SgfI * February 2, 2017 14:35 .. AccI GT'mk_AC Cuts at: 1150 1150 Size: 4112 Acc65I G'GTAC_C Cuts at: 1128 1128 Size: 4112 AclI AA'CG_TT Cuts at: 3952 3952 Size: 4112 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1937 2369 3116 3384 1937 Size: 432 747 268 2665 Fragments arranged by size: 2665 747 432 268 AflIII A'CryG_T Cuts at: 1464 2568 3545 1464 Size: 1104 977 2031 Fragments arranged by size: 2031 1104 977 AgeI A'CCGG_T Cuts at: 2500 2500 Size: 4112 AleI CACnn'nnGTG Cuts at: 923 923 Size: 4112 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1084 1116 1084 Size: 32 4080 AlwNI CAG_nnn'CTG Cuts at: 565 1417 2984 565 Size: 852 1567 1693 Fragments arranged by size: 1693 1567 852 ApaLI G'TGCA_C Cuts at: 934 2882 934 Size: 1948 2164 ApoI r'AATT_y Cuts at: 1116 3465 3721 1116 Size: 2349 256 1507 Fragments arranged by size: 2349 1507 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4112 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4112 AvaI C'yCGr_G Cuts at: 185 296 1132 185 Size: 111 836 3165 Fragments arranged by size: 3165 836 111 BamHI G'GATC_C Cuts at: 1137 1137 Size: 4112 BanI G'GyrC_C Cuts at: 455 485 1128 1542 1790 1825 455 Size: 30 643 414 248 35 2742 Fragments arranged by size: 2742 643 414 248 35 30 BanII G_rGCy'C Cuts at: 589 1126 2156 589 Size: 537 1030 2545 Fragments arranged by size: 2545 1030 537 BbeI G_GCGC'C Cuts at: 1546 1794 1546 Size: 248 3864 BbsI GAAGACnn'nnnn_ Cuts at: 197 197 Size: 4112 BciVI GTATCCnnnnn_n' Cuts at: 2010 2771 4112 2010 Size: 761 1341 2010 Fragments arranged by size: 2010 1341 761 BclI T'GATC_A Cuts at: 1632 1632 Size: 4112 BglI GCCn_nnn'nGGC Cuts at: 145 562 145 Size: 417 3695 BglII A'GATC_T Cuts at: 1627 3664 1627 Size: 2037 2075 BlpI GC'TnA_GC Cuts at: 841 1224 841 Size: 383 3729 Bme1580I G_kGCm'C Cuts at: 488 938 1737 1830 2886 488 Size: 450 799 93 1056 1714 Fragments arranged by size: 1714 1056 799 450 93 BmgBI CAC'GTC Cuts at: 582 1100 582 Size: 518 3594 BmrI ACTGGGnnnn_n' Cuts at: 517 553 1324 1405 1740 3884 517 Size: 36 771 81 335 2144 745 Fragments arranged by size: 2144 771 745 335 81 36 BmtI G_CTAG'C Cuts at: 412 412 Size: 4112 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2479 2479 Size: 4112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 232 1093 232 Size: 861 3251 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 343 994 1288 2167 2659 2957 343 Size: 651 294 879 492 298 1498 Fragments arranged by size: 1498 879 651 492 298 294 BsaI GGTCTCn'nnnn_ Cuts at: 703 3816 703 Size: 3113 999 BsaAI yAC'GTr Cuts at: 1465 1465 Size: 4112 BsaBI GATnn'nnATC Cuts at: 6 779 1626 3663 6 Size: 773 847 2037 455 Fragments arranged by size: 2037 847 773 455 BsaHI Gr'CG_yC Cuts at: 346 962 1543 1791 2493 3989 346 Size: 616 581 248 702 1496 469 Fragments arranged by size: 1496 702 616 581 469 248 BsaWI w'CCGG_w Cuts at: 1301 1822 2500 2774 2921 3332 1301 Size: 521 678 274 147 411 2081 Fragments arranged by size: 2081 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 571 601 571 Size: 30 4082 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 388 414 655 388 Size: 26 241 3845 Fragments arranged by size: 3845 241 26 BseYI C'CCAG_C Cuts at: 1537 2295 2872 3368 1537 Size: 758 577 496 2281 Fragments arranged by size: 2281 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1654 1700 2908 104 Size: 1550 46 1208 1308 Fragments arranged by size: 1550 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 938 1126 1904 2094 2886 938 Size: 188 778 190 792 2164 Fragments arranged by size: 2164 792 778 190 188 BsmBI CGTCTCn'nnnn_ Cuts at: 610 610 Size: 4112 Bsp1286I G_dGCh'C Cuts at: 488 589 938 1126 1737 1830 1904 2094 2156 Size: 101 349 188 611 93 74 190 62 Cuts at: 2156 2886 488 Size: 730 1714 Fragments arranged by size: 1714 730 611 349 190 188 101 93 74 62 BspEI T'CCGG_A Cuts at: 3332 3332 Size: 4112 BspHI T'CATG_A Cuts at: 3288 3288 Size: 4112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1162 1376 1678 2059 1162 Size: 214 302 381 3215 Fragments arranged by size: 3215 381 302 214 BsrBI CCG'CTC Cuts at: 50 2404 2458 3942 4107 50 Size: 2354 54 1484 165 55 Fragments arranged by size: 2354 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 695 938 2024 695 Size: 243 1086 2783 Fragments arranged by size: 2783 1086 243 BsrFI r'CCGG_y Cuts at: 488 943 2110 2291 2500 488 Size: 455 1167 181 209 2100 Fragments arranged by size: 2100 1167 455 209 181 BssSI C'ACGA_G Cuts at: 2383 2741 2383 Size: 358 3754 BstAPI GCAn_nnn'nTGC Cuts at: 492 1489 492 Size: 997 3115 BstBI TT'CG_AA Cuts at: 2473 2473 Size: 4112 BstXI CCAn_nnnn'nTGG Cuts at: 909 909 Size: 4112 BstYI r'GATC_y Cuts at: 1 390 1137 1604 1627 1962 2208 3220 3306 Size: 389 747 467 23 335 246 1012 86 Cuts at: 3306 3318 3664 1 Size: 12 346 449 Fragments arranged by size: 1012 747 467 449 389 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 451 451 Size: 4112 BtgI C'CryG_G Cuts at: 107 862 887 1016 107 Size: 755 25 129 3203 Fragments arranged by size: 3203 755 129 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2062 2243 2406 2062 Size: 181 163 3768 Fragments arranged by size: 3768 181 163 BtsI GCAGTG_nn' Cuts at: 1355 1355 Size: 4112 DraI TTT'AAA Cuts at: 1111 1111 Size: 4112 DrdI GACnn_nn'nnGTC Cuts at: 1818 2676 1818 Size: 858 3254 EaeI y'GGCC_r Cuts at: 137 899 1055 1697 1871 2262 2289 3634 137 Size: 762 156 642 174 391 27 1345 615 Fragments arranged by size: 1345 762 642 615 391 174 156 27 EagI C'GGCC_G Cuts at: 1697 1697 Size: 4112 EarI CTCTTCn'nnn_ Cuts at: 613 2135 2345 613 Size: 1522 210 2380 Fragments arranged by size: 2380 1522 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2320 2630 2776 3922 2320 Size: 310 146 1146 2510 Fragments arranged by size: 2510 1146 310 146 EcoICRI GAG'CTC Cuts at: 1124 1124 Size: 4112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 232 1093 1937 2369 3116 3384 232 Size: 861 844 432 747 268 960 Fragments arranged by size: 960 861 844 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 1050 1251 1050 Size: 201 3911 EcoRI G'AATT_C Cuts at: 1116 1116 Size: 4112 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2131 2131 Size: 4112 FspI TGC'GCA Cuts at: 1893 1893 Size: 4112 HaeII r_GCGC'y Cuts at: 1546 1794 2816 3795 1546 Size: 248 1022 979 1863 Fragments arranged by size: 1863 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 375 407 503 535 571 603 1954 1984 1986 Size: 32 96 32 36 32 1351 30 2 Cuts at: 1986 2016 375 Size: 30 2471 Fragments arranged by size: 2471 1351 96 36 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1151 1151 Size: 4112 KasI G'GCGC_C Cuts at: 1542 1790 1542 Size: 248 3864 KpnI G_GTAC'C Cuts at: 1132 1132 Size: 4112 MluI A'CGCG_T Cuts at: 3545 3545 Size: 4112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2783 2967 2783 Size: 184 3928 MscI TGG'CCA Cuts at: 139 901 1057 1873 139 Size: 762 156 816 2378 Fragments arranged by size: 2378 816 762 156 MslI CAynn'nnrTG Cuts at: 923 992 2101 2228 923 Size: 69 1109 127 2807 Fragments arranged by size: 2807 1109 127 69 MspA1I CmG'CkG Cuts at: 125 464 829 1223 1537 1897 2910 3155 125 Size: 339 365 394 314 360 1013 245 1082 Fragments arranged by size: 1082 1013 394 365 360 339 314 245 NaeI GCC'GGC Cuts at: 945 2293 945 Size: 1348 2764 NarI GG'CG_CC Cuts at: 1543 1791 1543 Size: 248 3864 NcoI C'CATG_G Cuts at: 107 1016 107 Size: 909 3203 NgoMIV G'CCGG_C Cuts at: 943 2291 943 Size: 1348 2764 NheI G'CTAG_C Cuts at: 408 408 Size: 4112 NspI r_CATG'y Cuts at: 1165 1665 2572 1165 Size: 500 907 2705 Fragments arranged by size: 2705 907 500 PciI A'CATG_T Cuts at: 2568 2568 Size: 4112 PflMI CCAn_nnn'nTGG Cuts at: 565 565 Size: 4112 PmeI GTTT'AAAC Cuts at: 1111 1111 Size: 4112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3251 3283 3251 Size: 32 4080 PstI C_TGCA'G Cuts at: 1159 1844 1159 Size: 685 3427 PvuI CG_AT'CG Cuts at: 104 104 Size: 4112 PvuII CAG'CTG Cuts at: 829 1537 1897 829 Size: 708 360 3044 Fragments arranged by size: 3044 708 360 RsrII CG'GwC_CG Cuts at: 2307 2307 Size: 4112 SacI G_AGCT'C Cuts at: 1126 1126 Size: 4112 SalI G'TCGA_C Cuts at: 1149 1149 Size: 4112 SapI GCTCTTCn'nnn_ Cuts at: 2135 2345 2135 Size: 210 3902 SbfI CC_TGCA'GG Cuts at: 1159 1159 Size: 4112 ScaI AGT'ACT Cuts at: 3629 3629 Size: 4112 SfcI C'TryA_G Cuts at: 33 1155 1840 2833 3024 33 Size: 1122 685 993 191 1121 Fragments arranged by size: 1122 1121 993 685 191 SfoI GGC'GCC Cuts at: 1544 1792 1544 Size: 248 3864 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4112 SmaI CCC'GGG Cuts at: 187 298 1134 187 Size: 111 836 3165 Fragments arranged by size: 3165 836 111 SmlI C'TyrA_G Cuts at: 322 1009 1267 2182 2674 2936 322 Size: 687 258 915 492 262 1498 Fragments arranged by size: 1498 915 687 492 262 258 SpeI A'CTAG_T Cuts at: 67 67 Size: 4112 SphI G_CATG'C Cuts at: 1165 1665 1165 Size: 500 3612 StuI AGG'CCT Cuts at: 271 271 Size: 4112 StyI C'CwwG_G Cuts at: 107 377 1016 1246 1600 107 Size: 270 639 230 354 2619 Fragments arranged by size: 2619 639 354 270 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1695 1828 2287 2496 1695 Size: 133 459 209 3311 Fragments arranged by size: 3311 459 209 133 TatI w'GTAC_w Cuts at: 3627 3627 Size: 4112 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 653 685 3629 3661 653 Size: 32 2944 32 1104 Fragments arranged by size: 2944 1104 32 32 Tth111I GACn'n_nGTC Cuts at: 1909 1909 Size: 4112 XbaI T'CTAG_A Cuts at: 1143 1143 Size: 4112 XcmI CCAnnnn_n'nnnnTGG Cuts at: 147 894 1607 147 Size: 747 713 2652 Fragments arranged by size: 2652 747 713 XmaI C'CCGG_G Cuts at: 185 296 1132 185 Size: 111 836 3165 Fragments arranged by size: 3165 836 111 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI