Restriction map for pF1KE2560
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BanII       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaBI       
BsaI        BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       Bsp1286I    Bsp1407I    
BspDI       BspHI       BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HgaI        
HincII      HindII      HpaI        KpnI        KspI        
MaeIII      MamI        MfeI        MluI        MluNI       
MscI        MslI        MspA1I      MunI        MvaI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PmeI        PpuMI       
PshAI       PshBI       PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfaNI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XmnI        


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# ONE CUT ENZYMES (   64 enzymes) #
###################################

AccI          598  GTAGAC               GT^[AC][GT]AC
AccII          53  CGCG                 CG^CG
AccIII         60  TCCGGA               T^CCGGA
AciI           52  CCGC                 CCGC(-3/-1)
AclI          263  AACGTT               AA^CGTT
AcsI          276  AAATTT               [GA]^AATT[CT]
AcyI           48  GGCGCC               G[GA]^CG[CT]C
ApoI          276  AAATTT               [GA]^AATT[CT]
BanI           48  GGCGCC               G^G[CT][GA]CC
BbeI           48  GGCGCC               GGCGC^C
BbrPI         352  CACGTG               CAC^GTG
BbvI          153  GCAGC                GCAGC(8/12)
BglII         174  AGATCT               A^GATCT
BsaAI         352  CACGTG               [CT]AC^GT[GA]
BsaHI          48  GGCGCC               G[GA]^CG[CT]C
BsaJI          69  CCCGGG               C^C[ACGT][ACGT]GG
BsaWI          60  TCCGGA               [AT]^CCGG[AT]
BseAI          60  TCCGGA               T^CCGGA
BsmI          156  GCATTC               GAATGC(1/-1)
BspEI          60  TCCGGA               T^CCGGA
BspLU11I      192  ACATGT               A^CATGT
BsrI          346  ACTGG                ACTGG(1/-1)
BstUI          53  CGCG                 CG^CG
BstYI         174  AGATCT               [GA]^GATC[CT]
Cac8I          45  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI           49  GCGC                 GCG^C
DdeI          117  CTCAG                C^T[ACGT]AG
DpnII         175  GATC                 ^GATC
EarI          574  GAAGAG               CTCTTC(1/4)
EcoRV         511  GATATC               GAT^ATC
HaeII          48  GGCGCC               [GA]GCGC^[CT]
HhaI           49  GCGC                 GCG^C
Hin1I          48  GGCGCC               G[GA]^CG[CT]C
HinP1I         49  GCGC                 G^CGC
HindIII       100  AAGCTT               A^AGCTT
HphI          361  GGTGA                GGTGA(8/7)
KasI           48  GGCGCC               G^GCGCC
Ksp632I       574  GAAGAG               CTCTTC(1/4)
MboI          175  GATC                 ^GATC
MflI          174  AGATCT               [GA]^GATC[CT]
MroI           60  TCCGGA               T^CCGGA
MvnI           53  CGCG                 CG^CG
MwoI           45  GCTGGCGCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI           48  GGCGCC               GG^CGCC
NdeII         175  GATC                 ^GATC
NlaIII        193  CATG                 CATG^
NspI          192  ACATGT               [GA]CATG^[CT]
PaeR7I         94  CTCGAG               C^TCGAG
PmaCI         352  CACGTG               CAC^GTG
PmlI          352  CACGTG               CAC^GTG
Psp1406I      263  AACGTT               AA^CGTT
Sau3AI        175  GATC                 ^GATC
ScaI          256  AGTACT               AGT^ACT
SfcI          178  CTACAG               C^T[GA][CT]AG
SfoI           48  GGCGCC               GGC^GCC
SmaI           69  CCCGGG               CCC^GGG
SmlI           94  CTCGAG               C^T[CT][GA]AG
TaqI           95  TCGA                 T^CGA
TseI          153  GCAGC                G^C[AT]GC
TthHB8I        95  TCGA                 T^CGA
XhoI           94  CTCGAG               C^TCGAG
XhoII         174  AGATCT               [GA]^GATC[CT]
XmaCI          69  CCCGGG               C^CCGGG
XmaI           69  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AfaI          257  GTAC                 GT^AC
              318  GTAC                
AflIII        192  ACATGT               A^C[GA][CT]GT
              351  ACACGT              
AluI          101  AGCT                 AG^CT
              219  AGCT                
AvaI           69  CCCGGG               C^[CT]CG[GA]G
               94  CTCGAG              
BcnI           69  CCCGG                CC^[GC]GG
               70  CCGGG               
BfaI          374  CTAG                 C^TAG
              382  CTAG                
BsiYI          70  CCGGGCTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              610  CCACCTGAAGG         
BslI           70  CCGGGCTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              610  CCACCTGAAGG         
BsoBI          69  CCCGGG               C^[CT]CG[GA]G
               94  CTCGAG              
BssKI          69  CCCGG                ^CC[ACGT]GG
               70  CCGGG               
BstF5I         63  GGATG                GGATG(2/0)
              297  GGATG               
Cfr13I         79  GGCCC                G^G[ACGT]CC
              431  GGGCC               
DraI          235  TTTAAA               TTT^AAA
              558  TTTAAA              
Fnu4HI         51  GCCGC                GC^[ACGT]GC
              153  GCAGC               
FokI           63  GGATG                GGATG(9/13)
              297  GGATG               
HaeIII         79  GGCC                 GG^CC
              432  GGCC                
HapII          61  CCGG                 C^CGG
               70  CCGG                
HpaII          61  CCGG                 C^CGG
               70  CCGG                
ItaI           51  GCCGC                GC^[ACGT]GC
              153  GCAGC               
MaeI          374  CTAG                 C^TAG
              382  CTAG                
MnlI           58  CCTC                 CCTC(7/6)
                3  GAGG                
MspI           61  CCGG                 C^CGG
               70  CCGG                
NciI           69  CCCGG                CC^[GC]GG
               70  CCGGG               
PleI           92  GACTC                GAGTC(4/5)
              370  GACTC               
RsaI          257  GTAC                 GT^AC
              318  GTAC                
Sau96I         79  GGCCC                G^G[ACGT]CC
              431  GGGCC               
ScrFI          69  CCCGG                CC^[ACGT]GG
               70  CCGGG               
TfiI          106  GATTC                G^A[AT]TC
              416  GAATC               
Tsp509I       239  AATT                 ^AATT
              277  AATT                
TspRI         321  CACTG                CA[GC]TG[ACGT][ACGT]^
              345  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BsmAI       HinfI       MaeII       MboII       MseI        
NlaIV       Tru9I       
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