Restriction map for pF1KE6679
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaLI       
AscI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbvCI       BciVI       BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsiYI       BslI        BsmBI       BsmI        
Bsp1407I    BspEI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstUI       BstXI       BstYI       BstZ17I     CelII       
Cfr10I      CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MflI        MluI        MroI        MslI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsaI        RsrII       SacII       
SalI        ScaI        SexAI       SfoI        SgrAI       
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          277  GTAGAC               GT^[AC][GT]AC
AciI          735  GCGG                 CCGC(-3/-1)
AocI          889  CCTAAGG              CC^T[ACGT]AGG
AseI          436  ATTAAT               AT^TAAT
AsnI          436  ATTAAT               AT^TAAT
AspHI         941  GAGCTC               G[AT]GC[AT]^C
BbsI            3  GAAGAC               GAAGAC(2/6)
BcgI          499  GCAGCCTTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          896  GCCCCCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI           3  GAAGAC               GAAGAC(2/6)
BsaBI         711  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         564  GAGGAG               GAGGAG(10/8)
BsgI           39  GTGCAG               GTGCAG(16/14)
BsiHKAI       941  GAGCTC               G[AT]GC[AT]^C
BsmAI         488  GTCTC                GTCTC(1/5)
BspDI         862  ATCGAT               AT^CGAT
BspHI         548  TCATGA               T^CATGA
BsrDI         245  CATTGC               GCAATG(2/0)
BstEII        877  GGTGACC              G^GT[ACGT]ACC
BstPI         877  GGTGACC              G^GT[ACGT]ACC
Bsu36I        889  CCTAAGG              CC^T[ACGT]AGG
CfoI          968  GCGC                 GCG^C
ClaI          862  ATCGAT               AT^CGAT
EarI          212  GAAGAG               CTCTTC(1/4)
Eco81I        889  CCTAAGG              CC^T[ACGT]AGG
EcoO65I       877  GGTGACC              G^GT[ACGT]ACC
HaeII         967  GGCGCT               [GA]GCGC^[CT]
HgaI           78  GCGTC                GACGC(5/10)
HhaI          968  GCGC                 GCG^C
HinP1I        968  GCGC                 G^CGC
HincII        586  GTTGAC               GT[CT]^[GA]AC
HindII        586  GTTGAC               GT[CT]^[GA]AC
Ksp632I       212  GAAGAG               CTCTTC(1/4)
MaeII         346  ACGT                 A^CGT
MamI          711  GATGCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          632  CAATTG               C^AATTG
MseI          437  TTAA                 T^TAA
MspA1I         68  CAGCTG               C[AC]G^C[GT]G
MunI          632  CAATTG               C^AATTG
PshAI         880  GACCGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         436  ATTAAT               AT^TAAT
PvuII          68  CAGCTG               CAG^CTG
RcaI          548  TCATGA               T^CATGA
SacI          941  GAGCTC               GAGCT^C
SapI          212  GAAGAGC              GCTCTTC(1/4)
SbfI          916  CCTGCAGG             CCTGCA^GG
SfiI          895  GGCCCCCCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          680  CTCAAG               C^T[CT][GA]AG
Sse8387I      916  CCTGCAGG             CCTGCA^GG
Tru9I         437  TTAA                 T^TAA
XbaI          208  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI          717  GATCC                GGATC(4/5)
              235  GGATC               
BalI          147  TGGCCA               TGG^CCA
              441  TGGCCA              
BfaI          209  CTAG                 C^TAG
              296  CTAG                
BpmI          944  CTCCAG               CTGGAG(16/14)
              848  CTGGAG              
BsmFI         474  GGGAC                GGGAC(10/14)
              806  GTCCC               
BstBI         350  TTCGAA               TT^CGAA
              507  TTCGAA              
BstF5I        480  GGATG                GGATG(2/0)
              710  GGATG               
Cac8I         765  GCCAGC               GC[ACGT]^[ACGT]GC
              950  GCAAGC              
DdeI          182  CTCAG                C^T[ACGT]AG
              890  CTAAG               
EaeI          147  TGGCCA               [CT]^GGCC[GA]
              441  TGGCCA              
FokI          480  GGATG                GGATG(9/13)
              710  GGATG               
MaeI          209  CTAG                 C^TAG
              296  CTAG                
MaeIII        725  GTGAC                ^GT[ACGT]AC
              878  GTGAC               
MluNI         147  TGGCCA               TGG^CCA
              441  TGGCCA              
MscI          147  TGGCCA               TGG^CCA
              441  TGGCCA              
NlaIII        549  CATG                 CATG^
              757  CATG                
NspV          350  TTCGAA               TT^CGAA
              507  TTCGAA              
PstI           65  CTGCAG               CTGCA^G
              917  CTGCAG              
SfcI           65  CTGCAG               C^T[GA][CT]AG
              917  CTGCAG              
SfuI          350  TTCGAA               TT^CGAA
              507  TTCGAA              
Tsp45I        725  GTGAC                ^GT[GC]AC
              878  GTGAC               
TspRI         312  CAGTG                CA[GC]TG[ACGT][ACGT]^
              535  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AcsI        AluI        ApaI        ApoI        AvaI        
AvaII       BanII       BbvI        BcnI        BmyI        
BsaJI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstNI       Cfr13I      DpnII       DraII       
EcoO109I    EcoRII      Fnu4HI      HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MboI        
MboII       MnlI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIV       PpuMI       Sau3AI      
Sau96I      ScrFI       SfaNI       SmaI        TaqI        
TfiI        TseI        Tsp509I     TthHB8I     XmaCI       
XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com