Restriction map for pF1KE2635
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspLU11I    BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MfeI        MluI        MroI        MspA1I      
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
Tth111I     Van91I      XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AflII         691  CTTAAG               C^TTAAG
Asp700I       606  GAACCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          820  CTCGAG               C^[CT]CG[GA]G
AvaII         915  GGTCC                G^G[AT]CC
BalI          947  TGGCCA               TGG^CCA
BamHI        1092  GGATCC               G^GATCC
BanI          600  GGCACC               G^G[CT][GA]CC
BbsI          813  GAAGAC               GAAGAC(2/6)
BfrI          691  CTTAAG               C^TTAAG
BpmI          164  CTGGAG               CTGGAG(16/14)
BpuAI         813  GAAGAC               GAAGAC(2/6)
BsaBI        1114  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI         323  GGGAC                GGGAC(10/14)
BsoBI         820  CTCGAG               C^[CT]CG[GA]G
BspHI         524  TCATGA               T^CATGA
BspMI         411  ACCTGC               ACCTGC(4/8)
BsrBI         823  GAGCGG               CCGCTC(-3/-3)
BsrDI        1084  CATTGC               GCAATG(2/0)
BstAPI        658  GCAAACACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        145  GGTGACC              G^GT[ACGT]ACC
BstPI         145  GGTGACC              G^GT[ACGT]ACC
BstYI        1092  GGATCC               [GA]^GATC[CT]
Cac8I         835  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI          487  GCGC                 GCG^C
DpnII        1093  GATC                 ^GATC
DraI          316  TTTAAA               TTT^AAA
DsaI          944  CCATGG               C^C[GA][CT]GG
EaeI          947  TGGCCA               [CT]^GGCC[GA]
EarI          391  CTCTTC               CTCTTC(1/4)
EcoO65I       145  GGTGACC              G^GT[ACGT]ACC
EcoRI          59  GAATTC               G^AATTC
FauI          825  GCGGG                CCCGC(4/6)
HaeII         486  GGCGCT               [GA]GCGC^[CT]
HapII         898  CCGG                 C^CGG
HhaI          487  GCGC                 GCG^C
HinP1I        487  GCGC                 G^CGC
HpaII         898  CCGG                 C^CGG
Ksp632I       391  CTCTTC               CTCTTC(1/4)
MaeIII        146  GTGAC                ^GT[ACGT]AC
MamI         1114  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI         1093  GATC                 ^GATC
MflI         1092  GGATCC               [GA]^GATC[CT]
MluNI         947  TGGCCA               TGG^CCA
MscI          947  TGGCCA               TGG^CCA
MspI          898  CCGG                 C^CGG
NcoI          944  CCATGG               C^CATGG
NdeII        1093  GATC                 ^GATC
PaeR7I        820  CTCGAG               C^TCGAG
PshAI         881  GACGAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          347  CTGCAG               CTGCA^G
RcaI          524  TCATGA               T^CATGA
Sau3AI       1093  GATC                 ^GATC
SwaI          315  ATTTAAAT             ATTT^AAAT
Tsp45I        146  GTGAC                ^GT[GC]AC
XhoI          820  CTCGAG               C^TCGAG
XhoII        1092  GGATCC               [GA]^GATC[CT]
XmnI          606  GAACCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwI         1093  GATCC                GGATC(4/5)
             1092  GGATC               
BsiYI         818  CCCTCGAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              819  CCTCGAGCGGG         
BslI          818  CCCTCGAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              819  CCTCGAGCGGG         
BsmAI         101  GAGAC                GTCTC(1/5)
              829  GAGAC               
DdeI          515  CTCAG                C^T[ACGT]AG
              805  CTCAG               
DraII         438  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              518  AGGCCCT             
EcoO109I      438  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              518  AGGCCCT             
EcoT14I       935  CCAAGG               C^C[AT][AT]GG
              944  CCATGG              
MaeII         446  ACGT                 A^CGT
              893  ACGT                
MslI          477  CATGGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1022  CATCAGAATG          
MwoI          511  GCAGCTCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              658  GCAAACACTGC         
PleI          573  GAGTC                GAGTC(4/5)
              905  GAGTC               
SfcI          347  CTGCAG               C^T[GA][CT]AG
              590  CTACAG              
StyI          935  CCAAGG               C^C[AT][AT]GG
              944  CCATGG              
TaqI          243  TCGA                 T^CGA
              821  TCGA                
TspRI         593  CAGTG                CA[GC]TG[ACGT][ACGT]^
              663  CACTG               
TthHB8I       243  TCGA                 T^CGA
              821  TCGA                
XcmI          935  CCAAGGTGTCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              977  CCAAAGACATATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BsaJI       BssKI       BstF5I      BstNI       
Cfr13I      EcoRII      Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        MboII       MnlI        
MseI        MvaI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       SfaNI       SmlI        TfiI        
Tru9I       TseI        Tsp509I     
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