Restriction map for pF1KE2635
 (Linear) (Six-base) MAPSORT of: KIEE2635.seq  Check: 246  from: 1  to: 1170



KIEE2635.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     600    1170 
   Size:        251     349     570

  Fragments arranged by size: 

                 570     349     251
 
AflII C'TTAA_G

Cuts at:      0     691    1170 
   Size:        691     479
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     158     190    1170 
   Size:        158      32     980

  Fragments arranged by size: 

                 980     158      32
 
ApoI r'AATT_y

Cuts at:      0      59     193     236     313     994    1170 
   Size:         59     134      43      77     681     176

  Fragments arranged by size: 

                 681     176     134      77      59      43
 
AvaI C'yCGr_G

Cuts at:      0     820    1170 
   Size:        820     350
 
BamHI G'GATC_C

Cuts at:      0    1092    1170 
   Size:       1092      78
 
BanI G'GyrC_C

Cuts at:      0     600    1170 
   Size:        600     570
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     820    1170 
   Size:        820     350
 
BmgBI CAC'GTC

Cuts at:      0     447     894    1170 
   Size:        447     447     276

  Fragments arranged by size: 

                 447     447     276
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185    1170 
   Size:        185     985
 
Bpu10I CC'TnA_GC

Cuts at:      0     515     805    1170 
   Size:        515     290     365

  Fragments arranged by size: 

                 515     365     290
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     722    1118    1170 
   Size:        722     396      52

  Fragments arranged by size: 

                 722     396      52
 
BsaBI GATnn'nnATC

Cuts at:      0    1118    1170 
   Size:       1118      52
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     158     188    1170 
   Size:        158      30     982

  Fragments arranged by size: 

                 982     158      30
 
BspHI T'CATG_A

Cuts at:      0     524    1170 
   Size:        524     646
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     420    1170 
   Size:        420     750
 
BsrBI CCG'CTC

Cuts at:      0     825    1170 
   Size:        825     345
 
BsrDI GCAATG_nn'

Cuts at:      0    1083    1170 
   Size:       1083      87
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     664    1170 
   Size:        664     506
 
BstEII G'GTnAC_C

Cuts at:      0     145    1170 
   Size:        145    1025
 
BstYI r'GATC_y

Cuts at:      0    1092    1170 
   Size:       1092      78
 
BtgI C'CryG_G

Cuts at:      0     944    1170 
   Size:        944     226
 
BtsI GCAGTG_nn'

Cuts at:      0     662    1170 
   Size:        662     508
 
DraI TTT'AAA

Cuts at:      0     318    1170 
   Size:        318     852
 
EaeI y'GGCC_r

Cuts at:      0     947    1170 
   Size:        947     223
 
EarI CTCTTCn'nnn_

Cuts at:      0     397    1170 
   Size:        397     773
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       1     670    1170 
   Size:          1     669     500

  Fragments arranged by size: 

                 669     500       1
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     251     600    1170 
   Size:        185      66     349     570

  Fragments arranged by size: 

                 570     349     185      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0     439     519    1170 
   Size:        439      80     651

  Fragments arranged by size: 

                 651     439      80
 
EcoRI G'AATT_C

Cuts at:      0      59    1170 
   Size:         59    1111
 
HaeII r_GCGC'y

Cuts at:      0     490    1170 
   Size:        490     680
 
MscI TGG'CCA

Cuts at:      0     949    1170 
   Size:        949     221
 
MslI CAynn'nnrTG

Cuts at:      0     481    1026    1170 
   Size:        481     545     144

  Fragments arranged by size: 

                 545     481     144
 
NcoI C'CATG_G

Cuts at:      0     944    1170 
   Size:        944     226
 
PfoI T'CCnGG_A

Cuts at:      0     137    1170 
   Size:        137    1033
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     158     190    1170 
   Size:        158      32     980

  Fragments arranged by size: 

                 980     158      32
 
PshAI GACnn'nnGTC

Cuts at:      0     885    1170 
   Size:        885     285
 
PspXI vC'TCGA_Gb

Cuts at:      0     820    1170 
   Size:        820     350
 
PstI C_TGCA'G

Cuts at:      0     351    1170 
   Size:        351     819
 
SfcI C'TryA_G

Cuts at:      0     347     590    1170 
   Size:        347     243     580

  Fragments arranged by size: 

                 580     347     243
 
SmlI C'TyrA_G

Cuts at:      0     691     737     820    1133    1170 
   Size:        691      46      83     313      37

  Fragments arranged by size: 

                 691     313      83      46      37
 
StyI C'CwwG_G

Cuts at:      0     935     944    1170 
   Size:        935       9     226

  Fragments arranged by size: 

                 935     226       9
 
SwaI ATTT'AAAT

Cuts at:      0     318    1170 
   Size:        318     852
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     164    1134    1170 
   Size:        164     970      36

  Fragments arranged by size: 

                 970     164      36
 
TatI w'GTAC_w

Cuts at:      0     256     453    1170 
   Size:        256     197     717

  Fragments arranged by size: 

                 717     256     197
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     116     148     316     348    1170 
   Size:        116      32     168      32     822

  Fragments arranged by size: 

                 822     168     116      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     942     984    1170 
   Size:        942      42     186

  Fragments arranged by size: 

                 942     186      42
 
XhoI C'TCGA_G

Cuts at:      0     820    1170 
   Size:        820     350
 
XmnI GAAnn'nnTTC

Cuts at:      0     610    1170 
   Size:        610     560
 

 Enzymes that do cut:

     AcuI    AflII     AloI     ApoI     AvaI    BamHI     BanI     BbsI
    BmgBI     BpmI   Bpu10I    BpuEI    BsaBI    BsaXI    BspHI    BspMI
    BsrBI    BsrDI   BstAPI   BstEII    BstYI     BtgI     BtsI     DraI
     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRI    HaeII     MscI
     MslI     NcoI     PfoI     PpiI    PshAI    PspXI     PstI     SfcI
     SmlI     StyI     SwaI    TaqII     TatI     TstI     XcmI     XhoI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BplI     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrFI
    BsrGI   BssHII    BssSI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI    PpuMI     PsiI   PspOMI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI  Tth111I
     XbaI     XmaI     ZraI
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