Restriction map for
pF1KB0464
(Circular) (Six-base) MAPSORT of: pF1KB0464.seq Check: 4722 from: 1 to: 6071
pF1KB0464 6071 bp
With 182 enzymes: SgfI *
August 25, 2016 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1719 1719
Size: 6071
AccI GT'mk_AC
Cuts at: 3109 3109
Size: 6071
Acc65I G'GTAC_C
Cuts at: 3087 3087
Size: 6071
AclI AA'CG_TT
Cuts at: 5911 5911
Size: 6071
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 1249 1738 2603 3896 4328 5075 5343 319
Size: 930 489 865 1293 432 747 268 1047
Fragments arranged by size:
1293 1047 930 865 747 489 432 268
AflII C'TTAA_G
Cuts at: 1075 1075
Size: 6071
AflIII A'CryG_T
Cuts at: 1577 3423 4527 5504 1577
Size: 1846 1104 977 2144
Fragments arranged by size:
2144 1846 1104 977
AgeI A'CCGG_T
Cuts at: 1040 4459 1040
Size: 3419 2652
AhdI GACnn_n'nnGTC
Cuts at: 2450 2450
Size: 6071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1357 2899 1357
Size: 1542 4529
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1761 1793 1761
Size: 32 6039
AlwNI CAG_nnn'CTG
Cuts at: 990 1510 3376 4943 990
Size: 520 1866 1567 2118
Fragments arranged by size:
2118 1866 1567 520
ApaI G_GGCC'C
Cuts at: 2109 2109
Size: 6071
ApaLI G'TGCA_C
Cuts at: 1581 2154 4841 1581
Size: 573 2687 2811
Fragments arranged by size:
2811 2687 573
ApoI r'AATT_y
Cuts at: 247 291 1259 2685 2946 3033 3075 5424 5680
Size: 44 968 1426 261 87 42 2349 256
Cuts at: 5680 247
Size: 638
Fragments arranged by size:
2349 1426 968 638 261 256 87 44
42
AseI AT'TA_AT
Cuts at: 20 20
Size: 6071
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6071
AvaI C'yCGr_G
Cuts at: 3091 3091
Size: 6071
AvrII C'CTAG_G
Cuts at: 1053 2700 1053
Size: 1647 4424
BamHI G'GATC_C
Cuts at: 637 3096 637
Size: 2459 3612
BanI G'GyrC_C
Cuts at: 1282 2263 3087 3501 3749 3784 1282
Size: 981 824 414 248 35 3569
Fragments arranged by size:
3569 981 824 414 248 35
BanII G_rGCy'C
Cuts at: 414 1621 2109 2244 2698 3085 4115 414
Size: 1207 488 135 454 387 1030 2370
Fragments arranged by size:
2370 1207 1030 488 454 387 135
BbeI G_GCGC'C
Cuts at: 3505 3753 3505
Size: 248 5823
BbsI GAAGACnn'nnnn_
Cuts at: 623 1403 2524 2801 623
Size: 780 1121 277 3893
Fragments arranged by size:
3893 1121 780 277
BbvCI CC'TCA_GC
Cuts at: 1012 1741 2012 1012
Size: 729 271 5071
Fragments arranged by size:
5071 729 271
BciVI GTATCCnnnnn_n'
Cuts at: 3969 4730 6071 3969
Size: 761 1341 3969
Fragments arranged by size:
3969 1341 761
BclI T'GATC_A
Cuts at: 1688 3591 1688
Size: 1903 4168
BglII A'GATC_T
Cuts at: 2557 2816 3586 5623 2557
Size: 259 770 2037 3005
Fragments arranged by size:
3005 2037 770 259
BlpI GC'TnA_GC
Cuts at: 1070 3183 1070
Size: 2113 3958
Bme1580I G_kGCm'C
Cuts at: 1431 1585 2109 2123 2158 3696 3789 4845 1431
Size: 154 524 14 35 1538 93 1056 2657
Fragments arranged by size:
2657 1538 1056 524 154 93 35 14
BmgBI CAC'GTC
Cuts at: 2478 2478
Size: 6071
BmrI ACTGGGnnnn_n'
Cuts at: 1333 1564 1995 3283 3364 3699 5843 1333
Size: 231 431 1288 81 335 2144 1561
Fragments arranged by size:
2144 1561 1288 431 335 231 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 433 1851 2541 4438 433
Size: 1418 690 1897 2066
Fragments arranged by size:
2066 1897 1418 690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 961 1452 1696 1821 1909 2258 484
Size: 477 491 244 125 88 349 4297
Fragments arranged by size:
4297 491 477 349 244 125 88
Bpu10I CC'TnA_GC
Cuts at: 1012 1741 2012 1012
Size: 729 271 5071
Fragments arranged by size:
5071 729 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3247 4126 4618 4916 3247
Size: 879 492 298 4402
Fragments arranged by size:
4402 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1239 2133 5775 1239
Size: 894 3642 1535
Fragments arranged by size:
3642 1535 894
BsaAI yAC'GTr
Cuts at: 1578 3424 1578
Size: 1846 4225
BsaBI GATnn'nnATC
Cuts at: 6 2186 2666 3585 5622 6
Size: 2180 480 919 2037 455
Fragments arranged by size:
2180 2037 919 480 455
BsaHI Gr'CG_yC
Cuts at: 3502 3750 4452 5948 3502
Size: 248 702 1496 3625
Fragments arranged by size:
3625 1496 702 248
BsaWI w'CCGG_w
Cuts at: 597 1040 3260 3781 4459 4733 4880 5291 597
Size: 443 2220 521 678 274 147 411 1377
Fragments arranged by size:
2220 1377 678 521 443 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2113 2143 2394 2424 2889 2919 2113
Size: 30 251 30 465 30 5265
Fragments arranged by size:
5265 465 251 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1482 1750 2121 1482
Size: 268 371 5432
Fragments arranged by size:
5432 371 268
BseYI C'CCAG_C
Cuts at: 748 790 1485 1510 2025 2986 3496 4254 4831
Size: 42 695 25 515 961 510 758 577
Cuts at: 4831 5327 748
Size: 496 1492
Fragments arranged by size:
1492 961 758 695 577 515 510 496
42 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 2144 1543
Size: 601 5470
BsiEI CG_ry'CG
Cuts at: 104 3613 3659 4867 104
Size: 3509 46 1208 1308
Fragments arranged by size:
3509 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1585 2158 2178 3085 3863 4053 4845 1585
Size: 573 20 907 778 190 792 2811
Fragments arranged by size:
2811 907 792 778 573 190 20
BsmI GAATG_Cn'
Cuts at: 129 2016 129
Size: 1887 4184
Bsp1286I G_dGCh'C
Cuts at: 414 1431 1585 1621 2109 2123 2158 2178 2244
Size: 1017 154 36 488 14 35 20 66
Cuts at: 2244 2698 3085 3696 3789 3863 4053 4115 4845
Size: 454 387 611 93 74 190 62 730
Cuts at: 4845 414
Size: 1640
Fragments arranged by size:
1640 1017 730 611 488 454 387 190
154 93 74 66 62 36 35 20
14
BspEI T'CCGG_A
Cuts at: 597 5291 597
Size: 4694 1377
BspHI T'CATG_A
Cuts at: 1598 5247 1598
Size: 3649 2422
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1719 3121 3335 3637 4018 1719
Size: 1402 214 302 381 3772
Fragments arranged by size:
3772 1402 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4363 4417 5901 6066 50
Size: 4313 54 1484 165 55
Fragments arranged by size:
4313 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2883 3983 2883
Size: 1100 4971
BsrFI r'CCGG_y
Cuts at: 1040 1304 1902 4069 4250 4459 1040
Size: 264 598 2167 181 209 2652
Fragments arranged by size:
2652 2167 598 264 209 181
BsrGI T'GTAC_A
Cuts at: 2298 2298
Size: 6071
BssSI C'ACGA_G
Cuts at: 4342 4700 4342
Size: 358 5713
BstAPI GCAn_nnn'nTGC
Cuts at: 135 3448 135
Size: 3313 2758
BstBI TT'CG_AA
Cuts at: 1257 4432 1257
Size: 3175 2896
BstEII G'GTnAC_C
Cuts at: 523 523
Size: 6071
BstXI CCAn_nnnn'nTGG
Cuts at: 834 1933 834
Size: 1099 4972
BstYI r'GATC_y
Cuts at: 1 637 699 2557 2630 2816 3096 3563 3586
Size: 636 62 1858 73 186 280 467 23
Cuts at: 3586 3921 4167 5179 5265 5277 5623 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1858 1012 636 467 449 346 335 280
246 186 86 73 62 23 12
BtgI C'CryG_G
Cuts at: 548 2918 3041 548
Size: 2370 123 3578
Fragments arranged by size:
3578 2370 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 599 4021 4202 4365 599
Size: 3422 181 163 2305
Fragments arranged by size:
3422 2305 181 163
BtsI GCAGTG_nn'
Cuts at: 365 2156 3067 3314 365
Size: 1791 911 247 3122
Fragments arranged by size:
3122 1791 911 247
ClaI AT'CG_AT
Cuts at: 3002 3002
Size: 6071
DraI TTT'AAA
Cuts at: 3070 3070
Size: 6071
DrdI GACnn_nn'nnGTC
Cuts at: 1504 3777 4635 1504
Size: 2273 858 2940
Fragments arranged by size:
2940 2273 858
EaeI y'GGCC_r
Cuts at: 2184 3656 3830 4221 4248 5593 2184
Size: 1472 174 391 27 1345 2662
Fragments arranged by size:
2662 1472 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3656 3656
Size: 6071
EarI CTCTTCn'nnn_
Cuts at: 563 1119 1191 1422 1820 2331 2622 2738 2806
Size: 556 72 231 398 511 291 116 68
Cuts at: 2806 2868 4094 4304 563
Size: 62 1226 210 2330
Fragments arranged by size:
2330 1226 556 511 398 291 231 210
116 72 68 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2930 4279 4589 4735 5881 2930
Size: 1349 310 146 1146 3120
Fragments arranged by size:
3120 1349 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3083 3083
Size: 6071
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 484 961 1249 1452 1696 1738 1821 1909
Size: 165 477 288 203 244 42 83 88
Cuts at: 1909 2258 2603 3896 4328 5075 5343 319
Size: 349 345 1293 432 747 268 1047
Fragments arranged by size:
1293 1047 747 477 432 349 345 288
268 244 203 165 88 83 42
EcoNI CCTnn'n_nnAGG
Cuts at: 674 1277 674
Size: 603 5468
EcoO109I rG'GnC_Cy
Cuts at: 191 870 1514 2105 2827 3210 191
Size: 679 644 591 722 383 3052
Fragments arranged by size:
3052 722 679 644 591 383
EcoRI G'AATT_C
Cuts at: 247 1259 3033 3075 247
Size: 1012 1774 42 3243
Fragments arranged by size:
3243 1774 1012 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1090 4090 1090
Size: 3000 3071
FspI TGC'GCA
Cuts at: 3852 3852
Size: 6071
HaeII r_GCGC'y
Cuts at: 2969 3505 3753 4775 5754 2969
Size: 536 248 1022 979 3286
Fragments arranged by size:
3286 1022 979 536 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 666 698 724 756 2436 2468 2761 2793 3913
Size: 32 26 32 1680 32 293 32 1120
Cuts at: 3913 3943 3945 3975 666
Size: 30 2 30 2762
Fragments arranged by size:
2762 1680 1120 293 32 32 32 32
30 30 26 2
HincII GTy'rAC
Cuts at: 3110 3110
Size: 6071
HindIII A'AGCT_T
Cuts at: 359 892 2196 2892 359
Size: 533 1304 696 3538
Fragments arranged by size:
3538 1304 696 533
KasI G'GCGC_C
Cuts at: 3501 3749 3501
Size: 248 5823
KpnI G_GTAC'C
Cuts at: 3091 3091
Size: 6071
MluI A'CGCG_T
Cuts at: 5504 5504
Size: 6071
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 409 1460 2596 4742 4926 409
Size: 1051 1136 2146 184 1554
Fragments arranged by size:
2146 1554 1136 1051 184
MscI TGG'CCA
Cuts at: 2186 3832 2186
Size: 1646 4425
MslI CAynn'nnrTG
Cuts at: 805 2756 4060 4187 805
Size: 1951 1304 127 2689
Fragments arranged by size:
2689 1951 1304 127
MspA1I CmG'CkG
Cuts at: 244 634 990 1794 1841 2025 3182 3496 3856
Size: 390 356 804 47 184 1157 314 360
Cuts at: 3856 4869 5114 244
Size: 1013 245 1201
Fragments arranged by size:
1201 1157 1013 804 390 360 356 314
245 184 47
NaeI GCC'GGC
Cuts at: 4252 4252
Size: 6071
NarI GG'CG_CC
Cuts at: 3502 3750 3502
Size: 248 5823
NcoI C'CATG_G
Cuts at: 2918 3041 2918
Size: 123 5948
NgoMIV G'CCGG_C
Cuts at: 4250 4250
Size: 6071
NspI r_CATG'y
Cuts at: 814 3124 3624 4531 814
Size: 2310 500 907 2354
Fragments arranged by size:
2354 2310 907 500
PasI CC'CwG_GG
Cuts at: 2109 2909 2109
Size: 800 5271
PciI A'CATG_T
Cuts at: 4527 4527
Size: 6071
PflMI CCAn_nnn'nTGG
Cuts at: 2924 2924
Size: 6071
PfoI T'CCnGG_A
Cuts at: 1319 2471 3008 1319
Size: 1152 537 4382
Fragments arranged by size:
4382 1152 537
PmeI GTTT'AAAC
Cuts at: 3070 3070
Size: 6071
PmlI CAC'GTG
Cuts at: 1578 1578
Size: 6071
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5210 5242 5210
Size: 32 6039
PpuMI rG'GwC_Cy
Cuts at: 191 870 1514 191
Size: 679 644 4748
Fragments arranged by size:
4748 679 644
PshAI GACnn'nnGTC
Cuts at: 2255 2255
Size: 6071
PspOMI G'GGCC_C
Cuts at: 2105 2105
Size: 6071
PstI C_TGCA'G
Cuts at: 372 1001 2793 2888 3118 3803 372
Size: 629 1792 95 230 685 2640
Fragments arranged by size:
2640 1792 685 629 230 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6071
PvuII CAG'CTG
Cuts at: 244 990 1841 3496 3856 244
Size: 746 851 1655 360 2459
Fragments arranged by size:
2459 1655 851 746 360
RsrII CG'GwC_CG
Cuts at: 4266 4266
Size: 6071
SacI G_AGCT'C
Cuts at: 3085 3085
Size: 6071
SalI G'TCGA_C
Cuts at: 3108 3108
Size: 6071
SanDI GG'GwC_CC
Cuts at: 1514 1514
Size: 6071
SapI GCTCTTCn'nnn_
Cuts at: 2738 4094 4304 2738
Size: 1356 210 4505
Fragments arranged by size:
4505 1356 210
SbfI CC_TGCA'GG
Cuts at: 3118 3118
Size: 6071
ScaI AGT'ACT
Cuts at: 5588 5588
Size: 6071
SexAI A'CCwGG_T
Cuts at: 527 527
Size: 6071
SfcI C'TryA_G
Cuts at: 33 368 997 2789 2884 3114 3799 4792 4983
Size: 335 629 1792 95 230 685 993 191
Cuts at: 4983 33
Size: 1121
Fragments arranged by size:
1792 1121 993 685 629 335 230 191
95
SfoI GGC'GCC
Cuts at: 3503 3751 3503
Size: 248 5823
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6071
SmaI CCC'GGG
Cuts at: 3093 3093
Size: 6071
SmlI C'TyrA_G
Cuts at: 1075 3226 4141 4633 4895 1075
Size: 2151 915 492 262 2251
Fragments arranged by size:
2251 2151 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6071
SphI G_CATG'C
Cuts at: 814 3124 3624 814
Size: 2310 500 3261
Fragments arranged by size:
3261 2310 500
StyI C'CwwG_G
Cuts at: 213 833 866 1053 2700 2918 3041 3205 3559
Size: 620 33 187 1647 218 123 164 354
Cuts at: 3559 213
Size: 2725
Fragments arranged by size:
2725 1647 620 354 218 187 164 123
33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 826 1401 1913 2085 3654 3787 4246 4455 826
Size: 575 512 172 1569 133 459 209 2442
Fragments arranged by size:
2442 1569 575 512 459 209 172 133
TatI w'GTAC_w
Cuts at: 908 1974 2298 5586 908
Size: 1066 324 3288 1393
Fragments arranged by size:
3288 1393 1066 324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2113 2145 2889 2921 5588 5620 2113
Size: 32 744 32 2667 32 2564
Fragments arranged by size:
2667 2564 744 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3868 3868
Size: 6071
XbaI T'CTAG_A
Cuts at: 3102 3102
Size: 6071
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1327 2925 3566 114
Size: 1213 1598 641 2619
Fragments arranged by size:
2619 1598 1213 641
XmaI C'CCGG_G
Cuts at: 3091 3091
Size: 6071
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AleI AscI BaeI BcgI BfrBI BglI
BmtI BsiWI BsmBI BssHII BstZ17I Bsu36I CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI