(Linear) (Six-base) MAPSORT of: KIBB0464.seq Check: 6136 from: 1 to: 2958 KIBB0464.orf With 182 enzymes: SgfI * August 25, 2016 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1611 2958 Size: 1611 1347 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 1141 1630 2495 2958 Size: 211 930 489 865 463 Fragments arranged by size: 930 865 489 463 211 AflII C'TTAA_G Cuts at: 0 967 2958 Size: 967 1991 AflIII A'CryG_T Cuts at: 0 1469 2958 Size: 1469 1489 AgeI A'CCGG_T Cuts at: 0 932 2958 Size: 932 2026 AhdI GACnn_n'nnGTC Cuts at: 0 2342 2958 Size: 2342 616 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1249 2791 2958 Size: 1249 1542 167 Fragments arranged by size: 1542 1249 167 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1653 1685 2958 Size: 1653 32 1273 Fragments arranged by size: 1653 1273 32 AlwNI CAG_nnn'CTG Cuts at: 0 882 1402 2958 Size: 882 520 1556 Fragments arranged by size: 1556 882 520 ApaI G_GGCC'C Cuts at: 0 2001 2958 Size: 2001 957 ApaLI G'TGCA_C Cuts at: 0 1473 2046 2958 Size: 1473 573 912 Fragments arranged by size: 1473 912 573 ApoI r'AATT_y Cuts at: 0 139 183 1151 2577 2838 2925 2958 Size: 139 44 968 1426 261 87 33 Fragments arranged by size: 1426 968 261 139 87 44 33 AvrII C'CTAG_G Cuts at: 0 945 2592 2958 Size: 945 1647 366 Fragments arranged by size: 1647 945 366 BamHI G'GATC_C Cuts at: 0 529 2958 Size: 529 2429 BanI G'GyrC_C Cuts at: 0 1174 2155 2958 Size: 1174 981 803 Fragments arranged by size: 1174 981 803 BanII G_rGCy'C Cuts at: 0 306 1513 2001 2136 2590 2958 Size: 306 1207 488 135 454 368 Fragments arranged by size: 1207 488 454 368 306 135 BbsI GAAGACnn'nnnn_ Cuts at: 0 515 1295 2416 2693 2958 Size: 515 780 1121 277 265 Fragments arranged by size: 1121 780 515 277 265 BbvCI CC'TCA_GC Cuts at: 0 904 1633 1904 2958 Size: 904 729 271 1054 Fragments arranged by size: 1054 904 729 271 BclI T'GATC_A Cuts at: 0 1580 2958 Size: 1580 1378 BglII A'GATC_T Cuts at: 0 2449 2708 2958 Size: 2449 259 250 Fragments arranged by size: 2449 259 250 BlpI GC'TnA_GC Cuts at: 0 962 2958 Size: 962 1996 Bme1580I G_kGCm'C Cuts at: 0 1323 1477 2001 2015 2050 2958 Size: 1323 154 524 14 35 908 Fragments arranged by size: 1323 908 524 154 35 14 BmgBI CAC'GTC Cuts at: 0 2370 2958 Size: 2370 588 BmrI ACTGGGnnnn_n' Cuts at: 0 1225 1456 1887 2958 Size: 1225 231 431 1071 Fragments arranged by size: 1225 1071 431 231 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 325 1743 2433 2958 Size: 325 1418 690 525 Fragments arranged by size: 1418 690 525 325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 853 1344 1588 1713 1801 2150 2958 Size: 376 477 491 244 125 88 349 808 Fragments arranged by size: 808 491 477 376 349 244 125 88 Bpu10I CC'TnA_GC Cuts at: 0 904 1633 1904 2958 Size: 904 729 271 1054 Fragments arranged by size: 1054 904 729 271 BsaI GGTCTCn'nnnn_ Cuts at: 0 1131 2025 2958 Size: 1131 894 933 Fragments arranged by size: 1131 933 894 BsaAI yAC'GTr Cuts at: 0 1470 2958 Size: 1470 1488 BsaBI GATnn'nnATC Cuts at: 0 2078 2558 2958 Size: 2078 480 400 Fragments arranged by size: 2078 480 400 BsaWI w'CCGG_w Cuts at: 0 489 932 2958 Size: 489 443 2026 Fragments arranged by size: 2026 489 443 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2005 2035 2286 2316 2781 2811 2958 Size: 2005 30 251 30 465 30 147 Fragments arranged by size: 2005 465 251 147 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1374 1642 2013 2958 Size: 1374 268 371 945 Fragments arranged by size: 1374 945 371 268 BseYI C'CCAG_C Cuts at: 0 640 682 1377 1402 1917 2878 2958 Size: 640 42 695 25 515 961 80 Fragments arranged by size: 961 695 640 515 80 42 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1435 2036 2958 Size: 1435 601 922 Fragments arranged by size: 1435 922 601 BsiHKAI G_wGCw'C Cuts at: 0 1477 2050 2070 2958 Size: 1477 573 20 888 Fragments arranged by size: 1477 888 573 20 BsmI GAATG_Cn' Cuts at: 0 21 1908 2958 Size: 21 1887 1050 Fragments arranged by size: 1887 1050 21 Bsp1286I G_dGCh'C Cuts at: 0 306 1323 1477 1513 2001 2015 2050 2070 Size: 306 1017 154 36 488 14 35 20 Cuts at: 2070 2136 2590 2958 Size: 66 454 368 Fragments arranged by size: 1017 488 454 368 306 154 66 36 35 20 14 BspEI T'CCGG_A Cuts at: 0 489 2958 Size: 489 2469 BspHI T'CATG_A Cuts at: 0 1490 2958 Size: 1490 1468 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1611 2958 Size: 1611 1347 BsrDI GCAATG_nn' Cuts at: 0 2775 2958 Size: 2775 183 BsrFI r'CCGG_y Cuts at: 0 932 1196 1794 2958 Size: 932 264 598 1164 Fragments arranged by size: 1164 932 598 264 BsrGI T'GTAC_A Cuts at: 0 2190 2958 Size: 2190 768 BstAPI GCAn_nnn'nTGC Cuts at: 0 27 2958 Size: 27 2931 BstBI TT'CG_AA Cuts at: 0 1149 2958 Size: 1149 1809 BstEII G'GTnAC_C Cuts at: 0 415 2958 Size: 415 2543 BstXI CCAn_nnnn'nTGG Cuts at: 0 726 1825 2958 Size: 726 1099 1133 Fragments arranged by size: 1133 1099 726 BstYI r'GATC_y Cuts at: 0 529 591 2449 2522 2708 2958 Size: 529 62 1858 73 186 250 Fragments arranged by size: 1858 529 250 186 73 62 BtgI C'CryG_G Cuts at: 0 440 2810 2933 2958 Size: 440 2370 123 25 Fragments arranged by size: 2370 440 123 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 491 2958 Size: 491 2467 BtsI GCAGTG_nn' Cuts at: 0 257 2048 2958 Size: 257 1791 910 Fragments arranged by size: 1791 910 257 ClaI AT'CG_AT Cuts at: 0 2894 2958 Size: 2894 64 DrdI GACnn_nn'nnGTC Cuts at: 0 1396 2958 Size: 1396 1562 EaeI y'GGCC_r Cuts at: 0 2076 2958 Size: 2076 882 EarI CTCTTCn'nnn_ Cuts at: 0 455 1011 1083 1314 1712 2223 2514 2630 Size: 455 556 72 231 398 511 291 116 Cuts at: 2630 2698 2760 2958 Size: 68 62 198 Fragments arranged by size: 556 511 455 398 291 231 198 116 72 68 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2822 2958 Size: 2822 136 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 376 853 1141 1344 1588 1630 1713 Size: 211 165 477 288 203 244 42 83 Cuts at: 1713 1801 2150 2495 2958 Size: 88 349 345 463 Fragments arranged by size: 477 463 349 345 288 244 211 203 165 88 83 42 EcoNI CCTnn'n_nnAGG Cuts at: 0 566 1169 2958 Size: 566 603 1789 Fragments arranged by size: 1789 603 566 EcoO109I rG'GnC_Cy Cuts at: 0 83 762 1406 1997 2719 2958 Size: 83 679 644 591 722 239 Fragments arranged by size: 722 679 644 591 239 83 EcoRI G'AATT_C Cuts at: 0 139 1151 2925 2958 Size: 139 1012 1774 33 Fragments arranged by size: 1774 1012 139 33 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 982 2958 Size: 982 1976 HaeII r_GCGC'y Cuts at: 0 2861 2958 Size: 2861 97 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 558 590 616 648 2328 2360 2653 2685 Size: 558 32 26 32 1680 32 293 32 Cuts at: 2685 2958 Size: 273 Fragments arranged by size: 1680 558 293 273 32 32 32 32 26 HindIII A'AGCT_T Cuts at: 0 251 784 2088 2784 2958 Size: 251 533 1304 696 174 Fragments arranged by size: 1304 696 533 251 174 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 301 1352 2488 2958 Size: 301 1051 1136 470 Fragments arranged by size: 1136 1051 470 301 MscI TGG'CCA Cuts at: 0 2078 2958 Size: 2078 880 MslI CAynn'nnrTG Cuts at: 0 697 2648 2958 Size: 697 1951 310 Fragments arranged by size: 1951 697 310 MspA1I CmG'CkG Cuts at: 0 136 526 882 1686 1733 1917 2958 Size: 136 390 356 804 47 184 1041 Fragments arranged by size: 1041 804 390 356 184 136 47 NcoI C'CATG_G Cuts at: 0 2810 2933 2958 Size: 2810 123 25 Fragments arranged by size: 2810 123 25 NspI r_CATG'y Cuts at: 0 706 2958 Size: 706 2252 PasI CC'CwG_GG Cuts at: 0 2001 2801 2958 Size: 2001 800 157 Fragments arranged by size: 2001 800 157 PflMI CCAn_nnn'nTGG Cuts at: 0 2816 2958 Size: 2816 142 PfoI T'CCnGG_A Cuts at: 0 1211 2363 2900 2958 Size: 1211 1152 537 58 Fragments arranged by size: 1211 1152 537 58 PmlI CAC'GTG Cuts at: 0 1470 2958 Size: 1470 1488 PpuMI rG'GwC_Cy Cuts at: 0 83 762 1406 2958 Size: 83 679 644 1552 Fragments arranged by size: 1552 679 644 83 PshAI GACnn'nnGTC Cuts at: 0 2147 2958 Size: 2147 811 PspOMI G'GGCC_C Cuts at: 0 1997 2958 Size: 1997 961 PstI C_TGCA'G Cuts at: 0 264 893 2685 2780 2958 Size: 264 629 1792 95 178 Fragments arranged by size: 1792 629 264 178 95 PvuII CAG'CTG Cuts at: 0 136 882 1733 2958 Size: 136 746 851 1225 Fragments arranged by size: 1225 851 746 136 SanDI GG'GwC_CC Cuts at: 0 1406 2958 Size: 1406 1552 SapI GCTCTTCn'nnn_ Cuts at: 0 2630 2958 Size: 2630 328 SexAI A'CCwGG_T Cuts at: 0 419 2958 Size: 419 2539 SfcI C'TryA_G Cuts at: 0 260 889 2681 2776 2958 Size: 260 629 1792 95 182 Fragments arranged by size: 1792 629 260 182 95 SmlI C'TyrA_G Cuts at: 0 967 2958 Size: 967 1991 SphI G_CATG'C Cuts at: 0 706 2958 Size: 706 2252 StyI C'CwwG_G Cuts at: 0 105 725 758 945 2592 2810 2933 2958 Size: 105 620 33 187 1647 218 123 25 Fragments arranged by size: 1647 620 218 187 123 105 33 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 718 1293 1805 1977 2958 Size: 718 575 512 172 981 Fragments arranged by size: 981 718 575 512 172 TatI w'GTAC_w Cuts at: 0 800 1866 2190 2958 Size: 800 1066 324 768 Fragments arranged by size: 1066 800 768 324 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2005 2037 2781 2813 2958 Size: 2005 32 744 32 145 Fragments arranged by size: 2005 744 145 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1219 2817 2958 Size: 1219 1598 141 Fragments arranged by size: 1598 1219 141 Enzymes that do cut: AarI AcuI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AvrII BamHI BanI BanII BbsI BbvCI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SanDI SapI SexAI SfcI SmlI SphI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AleI AscI AseI AsiSI AvaI BaeI BbeI BcgI BciVI BfrBI BglI BmtI BpuEI BsaHI BsiEI BsiWI BsmBI BsrBI BssHII BssSI BstZ17I Bsu36I CspCI DraI DraIII EagI EcoICRI EcoRV FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI