Restriction map for pF1KE3182
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        ApaI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaWI       BseAI       
BsgI        BsiWI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BsrDI       BsrGI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
ClaI        DraI        DraIII      DrdI        EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HindIII     HpaI        KasI        KpnI        MaeI        
MfeI        MroI        MseI        MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
SbfI        SexAI       SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp509I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccI           42  GTCGAC               GT^[AC][GT]AC
AcyI         1066  GACGCC               G[GA]^CG[CT]C
AfaI          543  GTAC                 GT^AC
AhdI          652  GACGGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        985  GTGCAC               G^TGCAC
AocI          931  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       365  AGCGCT               AGC^GCT
ApaLI         985  GTGCAC               G^TGCAC
AspEI         652  GACGGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1075  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          281  TGGCCA               TGG^CCA
BcgI           87  CGAGCCCGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         1158  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1158  GCTAAGC              GC^T[ACGT]AGC
BsaBI         885  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1066  GACGCC               G[GA]^CG[CT]C
BsaI          559  GAGACC               GGTCTC(1/5)
BseRI         184  GAGGAG               GAGGAG(10/8)
BsmBI         265  GAGACG               CGTCTC(1/5)
BspLU11I       38  ACATGT               A^CATGT
BspMI         323  ACCTGC               ACCTGC(4/8)
BsrFI         230  GCCGGC               [GA]^CCGG[CT]
BssHII        196  GCGCGC               G^CGCGC
BssSI         238  CACGAG               CACGAG(-5/-1)
BstAPI       1163  GCAGCCCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        931  CCTGAGG              CC^T[ACGT]AGG
CelII        1158  GCTAAGC              GC^T[ACGT]AGC
Cfr10I        230  GCCGGC               [GA]^CCGG[CT]
CpoI          662  CGGACCG              CG^G[AT]CCG
DraII         623  GGGGCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      652  GACGGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      365  AGCGCT               AGC^GCT
Eco81I        931  CCTGAGG              CC^T[ACGT]AGG
EcoNI         309  CCTGCGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      623  GGGGCCT              [GA]G^G[ACGT]CC[CT]
Hin1I        1066  GACGCC               G[GA]^CG[CT]C
HincII         42  GTCGAC               GT[CT]^[GA]AC
HindII         42  GTCGAC               GT[CT]^[GA]AC
HphI          872  TCACC                GGTGA(8/7)
KspI           15  CCGCGG               CCGC^GG
MamI          885  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1181  ACGCGT               A^CGCGT
MluNI         281  TGGCCA               TGG^CCA
MscI          281  TGGCCA               TGG^CCA
MslI          603  CACCTCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          230  GCCGGC               GCC^GGC
NgoMIV        230  GCCGGC               G^CCGGC
NspI           38  ACATGT               [GA]CATG^[CT]
PflFI        1075  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          178  CTGCAG               CTGCA^G
PvuII        1129  CAGCTG               CAG^CTG
RsaI          543  GTAC                 GT^AC
RsrII         662  CGGACCG              CG^G[AT]CCG
SacI          241  GAGCTC               GAGCT^C
SacII          15  CCGCGG               CCGC^GG
SalI           42  GTCGAC               G^TCGAC
ScaI          542  AGTACT               AGT^ACT
SfiI          616  GGCCTGGGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI          500  AGGCCT               AGG^CCT
Tth111I      1075  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          602  CCACCTCAGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AflIII         38  ACATGT               A^C[GA][CT]GT
             1181  ACGCGT              
AlwI          741  GATCC                GGATC(4/5)
              885  GATCC               
AviII         311  TGCGCA               TGC^GCA
              629  TGCGCA              
BanI           70  GGCACC               G^G[CT][GA]CC
              118  GGCACC              
BglI          617  GCCTGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              992  GCCTGGCAGGC         
BsiEI         232  CGGCCG               CG[GA][CT]^CG
              419  CGGCCG              
BsmAI         265  GAGAC                GTCTC(1/5)
              559  GAGAC               
BsrI          902  ACTGG                ACTGG(1/-1)
              921  CCAGT               
BstXI         272  CCATCAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              779  CCACCAACCTGG        
BstYI         740  AGATCC               [GA]^GATC[CT]
              884  AGATCC              
DpnII         741  GATC                 ^GATC
              885  GATC                
DsaI           15  CCGCGG               C^C[GA][CT]GG
              416  CCACGG              
EagI          232  CGGCCG               C^GGCCG
              419  CGGCCG              
EarI          244  CTCTTC               CTCTTC(1/4)
              576  CTCTTC              
EclXI         232  CGGCCG               C^GGCCG
              419  CGGCCG              
Eco52I        232  CGGCCG               C^GGCCG
              419  CGGCCG              
EcoT14I       513  CCAAGG               C^C[AT][AT]GG
              770  CCAAGG              
FspI          311  TGCGCA               TGC^GCA
              629  TGCGCA              
Ksp632I       244  CTCTTC               CTCTTC(1/4)
              576  CTCTTC              
MaeII         566  ACGT                 A^CGT
              791  ACGT                
MboI          741  GATC                 ^GATC
              885  GATC                
MflI          740  AGATCC               [GA]^GATC[CT]
              884  AGATCC              
MspA1I         15  CCGCGG               C[AC]G^C[GT]G
             1129  CAGCTG              
NdeII         741  GATC                 ^GATC
              885  GATC                
SapI          243  GCTCTTC              GCTCTTC(1/4)
              575  GCTCTTC             
Sau3AI        741  GATC                 ^GATC
              885  GATC                
SfcI          178  CTGCAG               C^T[GA][CT]AG
              472  CTACAG              
SmaI           74  CCCGGG               CCC^GGG
              288  CCCGGG              
StyI          513  CCAAGG               C^C[AT][AT]GG
              770  CCAAGG              
XhoII         740  AGATCC               [GA]^GATC[CT]
              884  AGATCC              
XmaCI          74  CCCGGG               C^CCGGG
              288  CCCGGG              
XmaI           74  CCCGGG               C^CCGGG
              288  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AspHI       
AvaI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiHKAI     BsiYI       
BslI        BsoBI       Bsp1286I    BsrBI       BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        EaeI        EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
ItaI        MaeIII      MboII       MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       PleI        Sau96I      ScrFI       SfaNI       
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com