Restriction map for pF1KE3182
 (Linear) (Six-base) MAPSORT of: KIEE3182.seq  Check: 2717  from: 1  to: 1257



KIEE3182.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AccI GT'mk_AC

Cuts at:      0      43    1257 
   Size:         43    1214
 
AfeI AGC'GCT

Cuts at:      0     367    1257 
   Size:        367     890
 
AflIII A'CryG_T

Cuts at:      0      38    1181    1257 
   Size:         38    1143      76

  Fragments arranged by size: 

                1143      76      38
 
AhdI GACnn_n'nnGTC

Cuts at:      0     657    1257 
   Size:        657     600
 
AleI CACnn'nnGTG

Cuts at:      0     607    1257 
   Size:        607     650
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     724    1257 
   Size:        724     533
 
AlwNI CAG_nnn'CTG

Cuts at:      0     480     744    1134    1257 
   Size:        480     264     390     123

  Fragments arranged by size: 

                 480     390     264     123
 
ApaLI G'TGCA_C

Cuts at:      0     985    1257 
   Size:        985     272
 
AvaI C'yCGr_G

Cuts at:      0      74     288     433    1257 
   Size:         74     214     145     824

  Fragments arranged by size: 

                 824     214     145      74
 
BanI G'GyrC_C

Cuts at:      0      70     118    1257 
   Size:         70      48    1139

  Fragments arranged by size: 

                1139      70      48
 
BanII G_rGCy'C

Cuts at:      0      92     245     440    1257 
   Size:         92     153     195     817

  Fragments arranged by size: 

                 817     195     153      92
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      76     110    1257 
   Size:         76      34    1147

  Fragments arranged by size: 

                1147      76      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     623     998    1257 
   Size:        623     375     259

  Fragments arranged by size: 

                 623     375     259
 
BlpI GC'TnA_GC

Cuts at:      0    1159    1257 
   Size:       1159      98
 
Bme1580I G_kGCm'C

Cuts at:      0      73     989    1257 
   Size:         73     916     268

  Fragments arranged by size: 

                 916     268      73
 
BmgBI CAC'GTC

Cuts at:      0     792    1257 
   Size:        792     465
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     915    1257 
   Size:        915     342
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     924    1257 
   Size:        211     713     333

  Fragments arranged by size: 

                 713     333     211
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     553    1257 
   Size:        553     704
 
BsaBI GATnn'nnATC

Cuts at:      0     889    1257 
   Size:        889     368
 
BsaHI Gr'CG_yC

Cuts at:      0    1067    1257 
   Size:       1067     190
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     199    1257 
   Size:        199    1058
 
BseYI C'CCAG_C

Cuts at:      0      24      64     612     676    1152    1257 
   Size:         24      40     548      64     476     105

  Fragments arranged by size: 

                 548     476     105      64      40      24
 
BsiEI CG_ry'CG

Cuts at:      0     235     422    1257 
   Size:        235     187     835

  Fragments arranged by size: 

                 835     235     187
 
BsiHKAI G_wGCw'C

Cuts at:      0     245     461     554     797     860     989    1257 
   Size:        245     216      93     243      63     129     268

  Fragments arranged by size: 

                 268     245     243     216     129      93      63
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     259    1257 
   Size:        259     998
 
Bsp1286I G_dGCh'C

Cuts at:      0      73      92     245     440     461     554     797     860 
   Size:         73      19     153     195      21      93     243      63

Cuts at:    860     989    1257 
   Size:        129     268

  Fragments arranged by size: 

                 268     243     195     153     129      93      73      63
                  21      19
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     332    1257 
   Size:        332     925
 
BsrBI CCG'CTC

Cuts at:      0     335     962    1010    1146    1257 
   Size:        335     627      48     136     111

  Fragments arranged by size: 

                 627     335     136     111      48
 
BsrFI r'CCGG_y

Cuts at:      0     230    1257 
   Size:        230    1027
 
BssHII G'CGCG_C

Cuts at:      0     196    1257 
   Size:        196    1061
 
BssSI C'ACGA_G

Cuts at:      0     238    1257 
   Size:        238    1019
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1169    1257 
   Size:       1169      88
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     279     786    1257 
   Size:        279     507     471

  Fragments arranged by size: 

                 507     471     279
 
BstYI r'GATC_y

Cuts at:      0     740     884    1257 
   Size:        740     144     373

  Fragments arranged by size: 

                 740     373     144
 
Bsu36I CC'TnA_GG

Cuts at:      0     932    1257 
   Size:        932     325
 
BtgI C'CryG_G

Cuts at:      0      15     416    1257 
   Size:         15     401     841

  Fragments arranged by size: 

                 841     401      15
 
BtsI GCAGTG_nn'

Cuts at:      0     552     687     987    1257 
   Size:        552     135     300     270

  Fragments arranged by size: 

                 552     300     270     135
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     954     989    1257 
   Size:        954      35     268

  Fragments arranged by size: 

                 954     268      35
 
EaeI y'GGCC_r

Cuts at:      0     232     269     281     389     419    1257 
   Size:        232      37      12     108      30     838

  Fragments arranged by size: 

                 838     232     108      37      30      12
 
EagI C'GGCC_G

Cuts at:      0     232     419    1257 
   Size:        232     187     838

  Fragments arranged by size: 

                 838     232     187
 
EarI CTCTTCn'nnn_

Cuts at:      0     250     582    1257 
   Size:        250     332     675

  Fragments arranged by size: 

                 675     332     250
 
EcoICRI GAG'CTC

Cuts at:      0     243    1257 
   Size:        243    1014
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     924    1257 
   Size:        211     713     333

  Fragments arranged by size: 

                 713     333     211
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     313    1257 
   Size:        313     944
 
EcoO109I rG'GnC_Cy

Cuts at:      0     624    1257 
   Size:        624     633
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     384    1257 
   Size:        384     873
 
FspI TGC'GCA

Cuts at:      0     313     631    1257 
   Size:        313     318     626

  Fragments arranged by size: 

                 626     318     313
 
HaeII r_GCGC'y

Cuts at:      0     170     369     446    1257 
   Size:        170     199      77     811

  Fragments arranged by size: 

                 811     199     170      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     643     675    1257 
   Size:        643      32     582

  Fragments arranged by size: 

                 643     582      32
 
HincII GTy'rAC

Cuts at:      0      44    1257 
   Size:         44    1213
 
MluI A'CGCG_T

Cuts at:      0    1181    1257 
   Size:       1181      76
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     674    1121    1257 
   Size:        641      33     447     136

  Fragments arranged by size: 

                 641     447     136      33
 
MscI TGG'CCA

Cuts at:      0     283    1257 
   Size:        283     974
 
MslI CAynn'nnrTG

Cuts at:      0     607    1257 
   Size:        607     650
 
MspA1I CmG'CkG

Cuts at:      0      17    1131    1257 
   Size:         17    1114     126

  Fragments arranged by size: 

                1114     126      17
 
NaeI GCC'GGC

Cuts at:      0     232    1257 
   Size:        232    1025
 
NgoMIV G'CCGG_C

Cuts at:      0     230    1257 
   Size:        230    1027
 
NspI r_CATG'y

Cuts at:      0      42    1257 
   Size:         42    1215
 
PciI A'CATG_T

Cuts at:      0      38    1257 
   Size:         38    1219
 
PstI C_TGCA'G

Cuts at:      0     182    1257 
   Size:        182    1075
 
PvuII CAG'CTG

Cuts at:      0    1131    1257 
   Size:       1131     126
 
RsrII CG'GwC_CG

Cuts at:      0     663    1257 
   Size:        663     594
 
SacI G_AGCT'C

Cuts at:      0     245    1257 
   Size:        245    1012
 
SacII CC_GC'GG

Cuts at:      0      18    1257 
   Size:         18    1239
 
SalI G'TCGA_C

Cuts at:      0      42    1257 
   Size:         42    1215
 
SapI GCTCTTCn'nnn_

Cuts at:      0     250     582    1257 
   Size:        250     332     675

  Fragments arranged by size: 

                 675     332     250
 
ScaI AGT'ACT

Cuts at:      0     544    1257 
   Size:        544     713
 
SfcI C'TryA_G

Cuts at:      0     178     472    1257 
   Size:        178     294     785

  Fragments arranged by size: 

                 785     294     178
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     623    1257 
   Size:        623     634
 
SmaI CCC'GGG

Cuts at:      0      76     290    1257 
   Size:         76     214     967

  Fragments arranged by size: 

                 967     214      76
 
StuI AGG'CCT

Cuts at:      0     502    1257 
   Size:        502     755
 
StyI C'CwwG_G

Cuts at:      0     513     770    1257 
   Size:        513     257     487

  Fragments arranged by size: 

                 513     487     257
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     615     680     849    1257 
   Size:        615      65     169     408

  Fragments arranged by size: 

                 615     408     169      65
 
TatI w'GTAC_w

Cuts at:      0     542    1257 
   Size:        542     715
 
Tth111I GACn'n_nGTC

Cuts at:      0    1078    1257 
   Size:       1078     179
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     609    1257 
   Size:        609     648
 
XmaI C'CCGG_G

Cuts at:      0      74     288    1257 
   Size:         74     214     969

  Fragments arranged by size: 

                 969     214      74
 

 Enzymes that do cut:

     AccI     AfeI   AflIII     AhdI     AleI     AlfI    AlwNI    ApaLI
     AvaI     BanI    BanII     BcgI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BpmI     BsaI    BsaBI    BsaHI    BseRI    BseYI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    HaeII    Hin4I   HincII     MluI     MmeI     MscI     MslI   MspA1I
     NaeI   NgoMIV     NspI     PciI     PstI    PvuII    RsrII     SacI
    SacII     SalI     SapI     ScaI     SfcI     SfiI     SmaI     StuI
     StyI    TaqII     TatI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AloI
     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI     BsgI    BsiWI
     BsmI    BspEI    BspHI    BsrDI    BsrGI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI     DraI   DraIII     DrdI     EciI    EcoRI    EcoRV
     FseI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    SanDI     SbfI    SexAI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI     XbaI     XhoI     XmnI     ZraI
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