Restriction map for pF1KE3355
##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AhdI        
Alw44I      AocI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AvrII       BciVI       BfrI        
BlnI        BsaBI       BseAI       BsiEI       BsiWI       
BsmI        BspDI       BspEI       BsrDI       BssHII      
BstAPI      BstEII      BstPI       Bsu36I      ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoO65I     EcoT22I     
HindIII     HpaI        KpnI        KspI        MamI        
MluI        MroI        NotI        NruI        NsiI        
PacI        PaeR7I      PmeI        PshAI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
ScaI        SgrAI       SnaBI       SpeI        SspI        
SwaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        2116  GACGTC               GACGT^C
AccII          65  CGCG                 CG^CG
AcsI          922  GAATTC               [GA]^AATT[CT]
AgeI         1618  ACCGGT               A^CCGGT
AlwNI        1687  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          922  GAATTC               [GA]^AATT[CT]
AseI         1870  ATTAAT               AT^TAAT
AsnI         1870  ATTAAT               AT^TAAT
AspI         1171  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2408  TGCGCA               TGC^GCA
BbrPI         800  CACGTG               CAC^GTG
BcgI          822  GCAGGACAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          884  TGATCA               T^GATCA
BglII        1604  AGATCT               A^GATCT
BsaI         1193  GAGACC               GGTCTC(1/5)
BsmBI        2716  GAGACG               CGTCTC(1/5)
Bsp1407I     2553  TGTACA               T^GTACA
BspHI        1580  TCATGA               T^CATGA
BspLU11I     1653  ACATGT               A^CATGT
BsrGI        2553  TGTACA               T^GTACA
BssSI        1799  CTCGTG               CACGAG(-5/-1)
Bst1107I     2667  GTATAC               GTA^TAC
BstBI        1085  TTCGAA               TT^CGAA
BstUI          65  CGCG                 CG^CG
BstZ17I      2667  GTATAC               GTA^TAC
DraIII        598  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         2275  GAAGAG               CTCTTC(1/4)
EcoRI         922  GAATTC               G^AATTC
EcoRV        1115  GATATC               GAT^ATC
FbaI          884  TGATCA               T^GATCA
FseI          811  GGCCGGCC             GGCCGG^CC
FspI         2408  TGCGCA               TGC^GCA
HincII       2768  GTCAAC               GT[CT]^[GA]AC
HindII       2768  GTCAAC               GT[CT]^[GA]AC
Ksp632I      2275  GAAGAG               CTCTTC(1/4)
MvnI           65  CGCG                 CG^CG
NaeI          812  GCCGGC               GCC^GGC
NdeI         1708  CATATG               CA^TATG
NgoMIV        812  GCCGGC               G^CCGGC
NheI         1462  GCTAGC               G^CTAGC
NspV         1085  TTCGAA               TT^CGAA
PflFI        1171  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1618  ACCGGT               A^CCGGT
PmaCI         800  CACGTG               CAC^GTG
PmlI          800  CACGTG               CAC^GTG
PshBI        1870  ATTAAT               AT^TAAT
RcaI         1580  TCATGA               T^CATGA
SapI         2275  GAAGAGC              GCTCTTC(1/4)
SexAI         599  ACCAGGT              A^CC[AT]GGT
SfiI         2749  GGCCTCCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         1085  TTCGAA               TT^CGAA
SphI         2102  GCATGC               GCATG^C
SspBI        2553  TGTACA               T^GTACA
Tth111I      1171  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1821  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI         2667  GTATAC               GT^[AC][GT]AC
             2704  GTCTAC              
AflIII        799  ACACGT               A^C[GA][CT]GT
             1653  ACATGT              
Aor51HI       247  AGCGCT               AGC^GCT
             2985  AGCGCT              
ApaI          624  GGGCCC               GGGCC^C
              975  GGGCCC              
AspHI        1969  GTGCTC               G[AT]GC[AT]^C
             2628  GAGCAC              
BalI          124  TGGCCA               TGG^CCA
             1994  TGGCCA              
BamHI        1019  GGATCC               G^GATCC
             1561  GGATCC              
BbsI          955  GAAGAC               GAAGAC(2/6)
              751  GTCTTC              
BglI          316  GCCTCTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2750  GCCTCCCAGGC         
BlpI          243  GCTGAGC              GC^T[ACGT]AGC
             2320  GCTGAGC             
Bpu1102I      243  GCTGAGC              GC^T[ACGT]AGC
             2320  GCTGAGC             
BpuAI         955  GAAGAC               GAAGAC(2/6)
              751  GTCTTC              
BsaAI         800  CACGTG               [CT]AC^GT[GA]
              888  CACGTA              
BsaWI        1618  ACCGGT               [AT]^CCGG[AT]
             2258  ACCGGA              
BseRI        1292  CTCCTC               GAGGAG(10/8)
             3003  CTCCTC              
BsiHKAI      1969  GTGCTC               G[AT]GC[AT]^C
             2628  GAGCAC              
BsrBI        2323  GAGCGG               CCGCTC(-3/-3)
             3115  GAGCGG              
CelII         243  GCTGAGC              GC^T[ACGT]AGC
             2320  GCTGAGC             
Eco47III      247  AGCGCT               AGC^GCT
             2985  AGCGCT              
MfeI          480  CAATTG               C^AATTG
             1370  CAATTG              
MluNI         124  TGGCCA               TGG^CCA
             1994  TGGCCA              
MscI          124  TGGCCA               TGG^CCA
             1994  TGGCCA              
MunI          480  CAATTG               C^AATTG
             1370  CAATTG              
NcoI         1187  CCATGG               C^CATGG
             1590  CCATGG              
PflMI        2329  CCAGACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2517  CCATTCAGTGG         
PvuII        2318  CAGCTG               CAG^CTG
             2857  CAGCTG              
SbfI          570  CCTGCAGG             CCTGCA^GG
             2139  CCTGCAGG            
SmlI          259  CTCAAG               C^T[CT][GA]AG
             1933  CTCAAG              
Sse8387I      570  CCTGCAGG             CCTGCA^GG
             2139  CCTGCAGG            
Van91I       2329  CCAGACTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2517  CCATTCAGTGG         
XcmI         1187  CCATGGGAGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1943  CCAACTGTGTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AvaI        AvaII       BanI        BanII       BbeI        
BbvCI       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NarI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmaCI       XmaI        
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