Restriction map for pF1KE3355
 (Linear) (Six-base) MAPSORT of: KIEE3355.seq  Check: 8061  from: 1  to: 3141



KIEE3355.orf

 With 182 enzymes: SgfI * 

                        August 27, 2018 19:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      31    3141 
   Size:         31    3110
 
AatII G_ACGT'C

Cuts at:      0    2120    3141 
   Size:       2120    1021
 
AccI GT'mk_AC

Cuts at:      0    2668    2705    3141 
   Size:       2668      37     436

  Fragments arranged by size: 

                2668     436      37
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     697    1055    2510    2574    2935    3010    3141 
   Size:        697     358    1455      64     361      75     131

  Fragments arranged by size: 

                1455     697     361     358     131      75      64
 
AfeI AGC'GCT

Cuts at:      0     249    2987    3141 
   Size:        249    2738     154

  Fragments arranged by size: 

                2738     249     154
 
AflIII A'CryG_T

Cuts at:      0     799    1653    3141 
   Size:        799     854    1488

  Fragments arranged by size: 

                1488     854     799
 
AgeI A'CCGG_T

Cuts at:      0    1618    3141 
   Size:       1618    1523
 
AleI CACnn'nnGTG

Cuts at:      0    1526    3141 
   Size:       1526    1615
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2653    3141 
   Size:       2653     488
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1692    3141 
   Size:       1692    1449
 
ApaI G_GGCC'C

Cuts at:      0     628     979    3141 
   Size:        628     351    2162

  Fragments arranged by size: 

                2162     628     351
 
ApoI r'AATT_y

Cuts at:      0     922    3141 
   Size:        922    2219
 
AseI AT'TA_AT

Cuts at:      0    1871    3141 
   Size:       1871    1270
 
AvaI C'yCGr_G

Cuts at:      0      58     611     628     836     950    1070    1233    3141 
   Size:         58     553      17     208     114     120     163    1908

  Fragments arranged by size: 

                1908     553     208     163     120     114      58      17
            
 
BamHI G'GATC_C

Cuts at:      0    1019    1561    3141 
   Size:       1019     542    1580

  Fragments arranged by size: 

                1580    1019     542
 
BanI G'GyrC_C

Cuts at:      0     217     237     344    1066    1750    2380    3141 
   Size:        217      20     107     722     684     630     761

  Fragments arranged by size: 

                 761     722     684     630     217     107      20
 
BanII G_rGCy'C

Cuts at:      0     628     951     979    1293    2310    3141 
   Size:        628     323      28     314    1017     831

  Fragments arranged by size: 

                1017     831     628     323     314      28
 
BbeI G_GCGC'C

Cuts at:      0     221     348    1754    3141 
   Size:        221     127    1406    1387

  Fragments arranged by size: 

                1406    1387     221     127
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     744     962    3141 
   Size:        744     218    2179

  Fragments arranged by size: 

                2179     744     218
 
BbvCI CC'TCA_GC

Cuts at:      0    2191    2561    2860    3141 
   Size:       2191     370     299     281

  Fragments arranged by size: 

                2191     370     299     281
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     811     845    3141 
   Size:        811      34    2296

  Fragments arranged by size: 

                2296     811      34
 
BclI T'GATC_A

Cuts at:      0     884    3141 
   Size:        884    2257
 
BglI GCCn_nnn'nGGC

Cuts at:      0     322    2756    3141 
   Size:        322    2434     385

  Fragments arranged by size: 

                2434     385     322
 
BglII A'GATC_T

Cuts at:      0    1604    3141 
   Size:       1604    1537
 
BlpI GC'TnA_GC

Cuts at:      0     244    2321    3141 
   Size:        244    2077     820

  Fragments arranged by size: 

                2077     820     244
 
Bme1580I G_kGCm'C

Cuts at:      0     177     476     628     979    1387    3141 
   Size:        177     299     152     351     408    1754

  Fragments arranged by size: 

                1754     408     351     299     177     152
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1139    2894    3141 
   Size:       1139    1755     247

  Fragments arranged by size: 

                1755    1139     247
 
BmtI G_CTAG'C

Cuts at:      0    1466    3141 
   Size:       1466    1675
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     709    1468    2491    3141 
   Size:        709     759    1023     650

  Fragments arranged by size: 

                1023     759     709     650
 
Bpu10I CC'TnA_GC

Cuts at:      0     106    2191    2309    2561    2860    3141 
   Size:        106    2085     118     252     299     281

  Fragments arranged by size: 

                2085     299     281     252     118     106
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244    1918    3141 
   Size:        244    1674    1223

  Fragments arranged by size: 

                1674    1223     244
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1187    3141 
   Size:       1187    1954
 
BsaAI yAC'GTr

Cuts at:      0     802     890    3141 
   Size:        802      88    2251

  Fragments arranged by size: 

                2251     802      88
 
BsaHI Gr'CG_yC

Cuts at:      0     218     345     557    1751    2117    3141 
   Size:        218     127     212    1194     366    1024

  Fragments arranged by size: 

                1194    1024     366     218     212     127
 
BsaWI w'CCGG_w

Cuts at:      0    1618    2258    3141 
   Size:       1618     640     883

  Fragments arranged by size: 

                1618     883     640
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2537    2567    2933    2963    3141 
   Size:       2537      30     366      30     178

  Fragments arranged by size: 

                2537     366     178      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1283    2994    3141 
   Size:       1283    1711     147

  Fragments arranged by size: 

                1711    1283     147
 
BseYI C'CCAG_C

Cuts at:      0     444     972    1484    2316    2899    3141 
   Size:        444     528     512     832     583     242

  Fragments arranged by size: 

                 832     583     528     512     444     242
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     613     809    1550    3141 
   Size:        613     196     741    1591

  Fragments arranged by size: 

                1591     741     613     196
 
BsiHKAI G_wGCw'C

Cuts at:      0    1973    2632    3141 
   Size:       1973     659     509

  Fragments arranged by size: 

                1973     659     509
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2710    3141 
   Size:       2710     431
 
Bsp1286I G_dGCh'C

Cuts at:      0     177     476     628     951     979    1293    1387    1973 
   Size:        177     299     152     323      28     314      94     586

Cuts at:   1973    2310    2632    3141 
   Size:        337     322     509

  Fragments arranged by size: 

                 586     509     337     323     322     314     299     177
                 152      94      28
 
BspHI T'CATG_A

Cuts at:      0    1580    3141 
   Size:       1580    1561
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      31     197     290    1677    2631    2680    3141 
   Size:         31     166      93    1387     954      49     461

  Fragments arranged by size: 

                1387     954     461     166      93      49      31
 
BsrBI CCG'CTC

Cuts at:      0    2325    3117    3141 
   Size:       2325     792      24

  Fragments arranged by size: 

                2325     792      24
 
BsrFI r'CCGG_y

Cuts at:      0     530     812    1618    3141 
   Size:        530     282     806    1523

  Fragments arranged by size: 

                1523     806     530     282
 
BsrGI T'GTAC_A

Cuts at:      0    2553    3141 
   Size:       2553     588
 
BssSI C'ACGA_G

Cuts at:      0    1799    3141 
   Size:       1799    1342
 
BstBI TT'CG_AA

Cuts at:      0    1086    3141 
   Size:       1086    2055
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1950    2391    2433    3141 
   Size:       1950     441      42     708

  Fragments arranged by size: 

                1950     708     441      42
 
BstYI r'GATC_y

Cuts at:      0     704    1019    1561    1604    2993    3141 
   Size:        704     315     542      43    1389     148

  Fragments arranged by size: 

                1389     704     542     315     148      43
 
BstZ17I GTA'TAC

Cuts at:      0    2669    3141 
   Size:       2669     472
 
BtgI C'CryG_G

Cuts at:      0     127     182    1187    1590    3141 
   Size:        127      55    1005     403    1551

  Fragments arranged by size: 

                1551    1005     403     127      55
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     981    1904    2313    3141 
   Size:        981     923     409     828

  Fragments arranged by size: 

                 981     923     828     409
 
BtsI GCAGTG_nn'

Cuts at:      0     175    2166    2954    3141 
   Size:        175    1991     788     187

  Fragments arranged by size: 

                1991     788     187     175
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2358    2393    3141 
   Size:       2358      35     748

  Fragments arranged by size: 

                2358     748      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     603    3141 
   Size:        603    2538
 
EaeI y'GGCC_r

Cuts at:      0     124    1994    2233    2245    2326    3141 
   Size:        124    1870     239      12      81     815

  Fragments arranged by size: 

                1870     815     239     124      81      12
 
EarI CTCTTCn'nnn_

Cuts at:      0    2270    3141 
   Size:       2270     871
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2827    3141 
   Size:       2827     314
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     697     709    1055    1468    2491    2510    2574    2935 
   Size:        697      12     346     413    1023      19      64     361

Cuts at:   2935    3010    3141 
   Size:         75     131

  Fragments arranged by size: 

                1023     697     413     361     346     131      75      64
                  19      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     712     859    1882    3141 
   Size:        712     147    1023    1259

  Fragments arranged by size: 

                1259    1023     712     147
 
EcoO109I rG'GnC_Cy

Cuts at:      0     136     292     567     624     625     793     852     976 
   Size:        136     156     275      57       1     168      59     124

Cuts at:    976    2181    2313    3124    3141 
   Size:       1205     132     811      17

  Fragments arranged by size: 

                1205     811     275     168     156     136     132     124
                  59      57      17       1
 
EcoRI G'AATT_C

Cuts at:      0     922    3141 
   Size:        922    2219
 
EcoRV GAT'ATC

Cuts at:      0    1117    3141 
   Size:       1117    2024
 
FseI GG_CCGG'CC

Cuts at:      0     816    3141 
   Size:        816    2325
 
FspI TGC'GCA

Cuts at:      0    2410    3141 
   Size:       2410     731
 
HaeII r_GCGC'y

Cuts at:      0       7     221     251     348    1754    2364    2989    3141 
   Size:          7     214      30      97    1406     610     625     152

  Fragments arranged by size: 

                1406     625     610     214     152      97      30       7
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     714     746    1321    1353    3141 
   Size:        714      32     575      32    1788

  Fragments arranged by size: 

                1788     714     575      32      32
 
HincII GTy'rAC

Cuts at:      0    2770    3141 
   Size:       2770     371
 
KasI G'GCGC_C

Cuts at:      0     217     344    1750    3141 
   Size:        217     127    1406    1391

  Fragments arranged by size: 

                1406    1391     217     127
 
MfeI C'AATT_G

Cuts at:      0     480    1370    3141 
   Size:        480     890    1771

  Fragments arranged by size: 

                1771     890     480
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1967    3141 
   Size:       1967    1174
 
MscI TGG'CCA

Cuts at:      0     126    1996    3141 
   Size:        126    1870    1145

  Fragments arranged by size: 

                1870    1145     126
 
MslI CAynn'nnrTG

Cuts at:      0    1526    2101    2635    3141 
   Size:       1526     575     534     506

  Fragments arranged by size: 

                1526     575     534     506
 
MspA1I CmG'CkG

Cuts at:      0     142     243    2232    2320    2859    3141 
   Size:        142     101    1989      88     539     282

  Fragments arranged by size: 

                1989     539     282     142     101      88
 
NaeI GCC'GGC

Cuts at:      0     814    3141 
   Size:        814    2327
 
NarI GG'CG_CC

Cuts at:      0     218     345    1751    3141 
   Size:        218     127    1406    1390

  Fragments arranged by size: 

                1406    1390     218     127
 
NcoI C'CATG_G

Cuts at:      0    1187    1590    3141 
   Size:       1187     403    1551

  Fragments arranged by size: 

                1551    1187     403
 
NdeI CA'TA_TG

Cuts at:      0    1709    3141 
   Size:       1709    1432
 
NgoMIV G'CCGG_C

Cuts at:      0     812    3141 
   Size:        812    2329
 
NheI G'CTAG_C

Cuts at:      0    1462    3141 
   Size:       1462    1679
 
NspI r_CATG'y

Cuts at:      0    1470    1657    2106    3141 
   Size:       1470     187     449    1035

  Fragments arranged by size: 

                1470    1035     449     187
 
PasI CC'CwG_GG

Cuts at:      0    1277    3141 
   Size:       1277    1864
 
PciI A'CATG_T

Cuts at:      0    1653    3141 
   Size:       1653    1488
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2335    2523    3141 
   Size:       2335     188     618

  Fragments arranged by size: 

                2335     618     188
 
PfoI T'CCnGG_A

Cuts at:      0    3129    3141 
   Size:       3129      12
 
PmlI CAC'GTG

Cuts at:      0     802    3141 
   Size:        802    2339
 
PpuMI rG'GwC_Cy

Cuts at:      0     136     292    2313    3124    3141 
   Size:        136     156    2021     811      17

  Fragments arranged by size: 

                2021     811     156     136      17
 
PspOMI G'GGCC_C

Cuts at:      0     624     975    3141 
   Size:        624     351    2166

  Fragments arranged by size: 

                2166     624     351
 
PstI C_TGCA'G

Cuts at:      0     575    1224    1499    2144    3141 
   Size:        575     649     275     645     997

  Fragments arranged by size: 

                 997     649     645     575     275
 
PvuII CAG'CTG

Cuts at:      0    2320    2859    3141 
   Size:       2320     539     282

  Fragments arranged by size: 

                2320     539     282
 
SanDI GG'GwC_CC

Cuts at:      0     136    3141 
   Size:        136    3005
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2270    3141 
   Size:       2270     871
 
SbfI CC_TGCA'GG

Cuts at:      0     575    2144    3141 
   Size:        575    1569     997

  Fragments arranged by size: 

                1569     997     575
 
SexAI A'CCwGG_T

Cuts at:      0     599    3141 
   Size:        599    2542
 
SfcI C'TryA_G

Cuts at:      0     571     806    1220    1495    2121    2140    3141 
   Size:        571     235     414     275     626      19    1001

  Fragments arranged by size: 

                1001     626     571     414     275     235      19
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2756    3141 
   Size:       2756     385
 
SfoI GGC'GCC

Cuts at:      0     219     346    1752    3141 
   Size:        219     127    1406    1389

  Fragments arranged by size: 

                1406    1389     219     127
 
SmaI CCC'GGG

Cuts at:      0      60     838     952    1072    3141 
   Size:         60     778     114     120    2069

  Fragments arranged by size: 

                2069     778     120     114      60
 
SmlI C'TyrA_G

Cuts at:      0     259    1933    3141 
   Size:        259    1674    1208

  Fragments arranged by size: 

                1674    1208     259
 
SphI G_CATG'C

Cuts at:      0    2106    3141 
   Size:       2106    1035
 
StuI AGG'CCT

Cuts at:      0     865    2464    2566    3141 
   Size:        865    1599     102     575

  Fragments arranged by size: 

                1599     865     575     102
 
StyI C'CwwG_G

Cuts at:      0    1187    1590    3014    3141 
   Size:       1187     403    1424     127

  Fragments arranged by size: 

                1424    1187     403     127
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     436     655    1175    1269    3141 
   Size:        436     219     520      94    1872

  Fragments arranged by size: 

                1872     520     436     219      94
 
TatI w'GTAC_w

Cuts at:      0    2553    3141 
   Size:       2553     588
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     128     160     477     509    2933    2965    3141 
   Size:        128      32     317      32    2424      32     176

  Fragments arranged by size: 

                2424     317     176     128      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1174    3141 
   Size:       1174    1967
 
XbaI T'CTAG_A

Cuts at:      0    1821    3141 
   Size:       1821    1320
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1194    1950    3141 
   Size:       1194     756    1191

  Fragments arranged by size: 

                1194    1191     756
 
XmaI C'CCGG_G

Cuts at:      0      58     836     950    1070    3141 
   Size:         58     778     114     120    2071

  Fragments arranged by size: 

                2071     778     120     114      58
 
ZraI GAC'GTC

Cuts at:      0    2118    3141 
   Size:       2118    1023
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AgeI     AleI
     AlfI    AlwNI     ApaI     ApoI     AseI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI    BssSI
    BstBI    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI    CspCI
   DraIII     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    HaeII    Hin4I   HincII     KasI     MfeI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NspI     PasI     PciI    PflMI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AhdI     AloI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BciVI    BfrBI    BmgBI     BplI    BsaBI    BsiEI
    BsiWI     BsmI    BspEI    BsrDI   BssHII   BstAPI   BstEII   Bsu36I
     ClaI     DraI     DrdI     EagI  EcoICRI     FalI    FspAI  HindIII
     HpaI     KpnI     MluI     NotI     NruI     NsiI     PacI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     ScaI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI     XhoI     XmnI
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