Restriction map for pF1KE3393
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BseAI       BsiEI       BsiWI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstPI       BstUI       CelII       Cfr10I      CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       FbaI        
FseI        FspI        HgaI        Hin1I       HindIII     
HpaI        KasI        KspI        MluI        MroI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    StuI        SwaI        Tth111I     XbaI        
XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   70 enzymes) #
###################################

Acc65I       3166  GGTACC               G^GTACC
AlwNI        3500  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI         3243  ATTAAT               AT^TAAT
AsnI         3243  ATTAAT               AT^TAAT
Asp718I      3166  GGTACC               G^GTACC
AspHI         721  GAGCTC               G[AT]GC[AT]^C
AvaI         1421  CTCGAG               C^[CT]CG[GA]G
BalI         2436  TGGCCA               TGG^CCA
BanII         721  GAGCTC               G[GA]GC[CT]^C
BcgI           42  CGATGTCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         570  GTATCC               GTATCC(6/5)
BmyI          721  GAGCTC               G[AGT]GC[ACT]^C
BsaBI        1018  GATCTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         3115  GGTCTC               GGTCTC(1/5)
BsaWI         711  ACCGGA               [AT]^CCGG[AT]
BsgI         2219  CTGCAC               GTGCAG(16/14)
BsiHKAI       721  GAGCTC               G[AT]GC[AT]^C
BsoBI        1421  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      721  GAGCTC               G[AGT]GC[ACT]^C
Bsp1407I     3183  TGTACA               T^GTACA
BspDI        1351  ATCGAT               AT^CGAT
BspMI        2976  ACCTGC               ACCTGC(4/8)
BsrDI        2842  GCAATG               GCAATG(2/0)
BsrGI        3183  TGTACA               T^GTACA
BssKI         736  CCAGG                ^CC[ACGT]GG
Bst1107I      264  GTATAC               GTA^TAC
BstNI         736  CCAGG                CC^[AT]GG
BstXI         140  CCACACAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       264  GTATAC               GTA^TAC
CfoI          501  GCGC                 GCG^C
ClaI         1351  ATCGAT               AT^CGAT
DraI         2010  TTTAAA               TTT^AAA
DraII         171  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          269  CCGTGG               C^C[GA][CT]GG
EaeI         2436  TGGCCA               [CT]^GGCC[GA]
EcoNI         123  CCTGAAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      171  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1131  GAATTC               G^AATTC
EcoRII        736  CCAGG                ^CC[AT]GG
EcoT22I      2760  ATGCAT               ATGCA^T
FauI         3472  GCGGG                CCCGC(4/6)
HaeII         500  AGCGCC               [GA]GCGC^[CT]
HapII         712  CCGG                 C^CGG
HhaI          501  GCGC                 GCG^C
HinP1I        501  GCGC                 G^CGC
HincII        246  GTTGAC               GT[CT]^[GA]AC
HindII        246  GTTGAC               GT[CT]^[GA]AC
HpaII         712  CCGG                 C^CGG
KpnI         3166  GGTACC               GGTAC^C
MaeII         306  ACGT                 A^CGT
MamI         1018  GATCTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          145  CAATTG               C^AATTG
MluNI        2436  TGGCCA               TGG^CCA
MscI         2436  TGGCCA               TGG^CCA
MspI          712  CCGG                 C^CGG
MunI          145  CAATTG               C^AATTG
MvaI          736  CCAGG                CC^[AT]GG
NsiI         2760  ATGCAT               ATGCA^T
NspI         2033  ACATGC               [GA]CATG^[CT]
PaeR7I       1421  CTCGAG               C^TCGAG
PflMI        3223  CCAAGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         171  GGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        3243  ATTAAT               AT^TAAT
SacI          721  GAGCTC               GAGCT^C
ScaI         1193  AGTACT               AGT^ACT
ScrFI         736  CCAGG                CC^[ACGT]GG
SpeI         2776  ACTAGT               A^CTAGT
SspBI        3183  TGTACA               T^GTACA
Van91I       3223  CCAAGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1421  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AccI          264  GTATAC               GT^[AC][GT]AC
              694  GTAGAC              
AciI            7  GCGG                 CCGC(-3/-1)
             3472  GCGG                
AocI          907  CCTGAGG              CC^T[ACGT]AGG
             1339  CCTGAGG             
BpmI          398  CTCCAG               CTGGAG(16/14)
              946  CTCCAG              
BsmBI         505  CGTCTC               CGTCTC(1/5)
              303  GAGACG              
Bsu36I        907  CCTGAGG              CC^T[ACGT]AGG
             1339  CCTGAGG             
Cac8I          18  GCCTGC               GC[ACGT]^[ACGT]GC
              751  GCAAGC              
Eco81I        907  CCTGAGG              CC^T[ACGT]AGG
             1339  CCTGAGG             
EcoT14I      1113  CCTTGG               C^C[AT][AT]GG
             2867  CCTTGG              
PstI         1526  CTGCAG               CTGCA^G
             3287  CTGCAG              
StyI         1113  CCTTGG               C^C[AT][AT]GG
             2867  CCTTGG              
Tsp45I       1067  GTGAC                ^GT[GC]AC
             1676  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AvaII       BanI        BbsI        BbvI        BfaI        
BglII       BpuAI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsrI        
BstF5I      BstYI       Cfr13I      DdeI        DpnII       
EarI        Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      NdeII       NlaIII      NlaIV       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
SfaNI       SfcI        SmlI        SspI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XhoII       
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