Restriction map for pF1KB5914
 (Linear) (Six-base) MAPSORT of: KIBB5914.seq  Check: 529  from: 1  to: 1590



KIBB5914.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AccI GT'mk_AC

Cuts at:      0     110    1590 
   Size:        110    1480
 
Acc65I G'GTAC_C

Cuts at:      0     674    1590 
   Size:        674     916
 
ApoI r'AATT_y

Cuts at:      0    1115    1590 
   Size:       1115     475
 
AvaI C'yCGr_G

Cuts at:      0     758     793    1590 
   Size:        758      35     797

  Fragments arranged by size: 

                 797     758      35
 
BanI G'GyrC_C

Cuts at:      0     251     674    1590 
   Size:        251     423     916

  Fragments arranged by size: 

                 916     423     251
 
BanII G_rGCy'C

Cuts at:      0     793     965    1570    1590 
   Size:        793     172     605      20

  Fragments arranged by size: 

                 793     605     172      20
 
BclI T'GATC_A

Cuts at:      0     842     926    1590 
   Size:        842      84     664

  Fragments arranged by size: 

                 842     664      84
 
BglI GCCn_nnn'nGGC

Cuts at:      0     210     730    1590 
   Size:        210     520     860

  Fragments arranged by size: 

                 860     520     210
 
BglII A'GATC_T

Cuts at:      0    1074    1590 
   Size:       1074     516
 
BlpI GC'TnA_GC

Cuts at:      0     824     964    1590 
   Size:        824     140     626

  Fragments arranged by size: 

                 824     626     140
 
Bme1580I G_kGCm'C

Cuts at:      0     254    1590 
   Size:        254    1336
 
BmgBI CAC'GTC

Cuts at:      0    1392    1590 
   Size:       1392     198
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     848    1450    1590 
   Size:        848     602     140

  Fragments arranged by size: 

                 848     602     140
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      99     452    1590 
   Size:         99     353    1138

  Fragments arranged by size: 

                1138     353      99
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     838    1055    1495    1590 
   Size:         94     744     217     440      95

  Fragments arranged by size: 

                 744     440     217      95      94
 
Bpu10I CC'TnA_GC

Cuts at:      0     787    1590 
   Size:        787     803
 
BsaAI yAC'GTr

Cuts at:      0    1494    1590 
   Size:       1494      96
 
BsaHI Gr'CG_yC

Cuts at:      0     782    1590 
   Size:        782     808
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1329    1590 
   Size:       1329     261
 
BseYI C'CCAG_C

Cuts at:      0     175     932    1343    1590 
   Size:        175     757     411     247

  Fragments arranged by size: 

                 757     411     247     175
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     700    1554    1590 
   Size:        700     854      36

  Fragments arranged by size: 

                 854     700      36
 
BsiHKAI G_wGCw'C

Cuts at:      0      80     809     965    1590 
   Size:         80     729     156     625

  Fragments arranged by size: 

                 729     625     156      80
 
BsmI GAATG_Cn'

Cuts at:      0     170     527    1491    1590 
   Size:        170     357     964      99

  Fragments arranged by size: 

                 964     357     170      99
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     254     793     809     965    1570    1590 
   Size:         80     174     539      16     156     605      20

  Fragments arranged by size: 

                 605     539     174     156      80      20      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1475    1590 
   Size:       1475     115
 
BsrBI CCG'CTC

Cuts at:      0    1331    1590 
   Size:       1331     259
 
BsrDI GCAATG_nn'

Cuts at:      0     186    1590 
   Size:        186    1404
 
BsrFI r'CCGG_y

Cuts at:      0     587     724    1590 
   Size:        587     137     866

  Fragments arranged by size: 

                 866     587     137
 
BsrGI T'GTAC_A

Cuts at:      0     571    1590 
   Size:        571    1019
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     599     804    1356    1590 
   Size:        599     205     552     234

  Fragments arranged by size: 

                 599     552     234     205
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1362    1590 
   Size:       1362     228
 
BstYI r'GATC_y

Cuts at:      0    1074    1590 
   Size:       1074     516
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     259    1590 
   Size:        259    1331
 
ClaI AT'CG_AT

Cuts at:      0     578    1590 
   Size:        578    1012
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     723     758    1590 
   Size:        723      35     832

  Fragments arranged by size: 

                 832     723      35
 
DraI TTT'AAA

Cuts at:      0     519    1590 
   Size:        519    1071
 
EaeI y'GGCC_r

Cuts at:      0     238     747     902    1590 
   Size:        238     509     155     688

  Fragments arranged by size: 

                 688     509     238     155
 
EcoICRI GAG'CTC

Cuts at:      0     963    1590 
   Size:        963     627
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     838    1055    1495    1590 
   Size:         94     744     217     440      95

  Fragments arranged by size: 

                 744     440     217      95      94
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     817    1590 
   Size:        817     773
 
EcoRI G'AATT_C

Cuts at:      0    1115    1590 
   Size:       1115     475
 
EcoRV GAT'ATC

Cuts at:      0     475    1590 
   Size:        475    1115
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1080    1590 
   Size:       1080     510
 
HaeII r_GCGC'y

Cuts at:      0    1038    1590 
   Size:       1038     552
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     451     483    1590 
   Size:        451      32    1107

  Fragments arranged by size: 

                1107     451      32
 
KpnI G_GTAC'C

Cuts at:      0     678    1590 
   Size:        678     912
 
MfeI C'AATT_G

Cuts at:      0    1040    1590 
   Size:       1040     550
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     982    1200    1590 
   Size:        982     218     390

  Fragments arranged by size: 

                 982     390     218
 
MscI TGG'CCA

Cuts at:      0     240    1590 
   Size:        240    1350
 
MslI CAynn'nnrTG

Cuts at:      0     293     883    1590 
   Size:        293     590     707

  Fragments arranged by size: 

                 707     590     293
 
MspA1I CmG'CkG

Cuts at:      0     714     932    1590 
   Size:        714     218     658

  Fragments arranged by size: 

                 714     658     218
 
NaeI GCC'GGC

Cuts at:      0     726    1590 
   Size:        726     864
 
NgoMIV G'CCGG_C

Cuts at:      0     724    1590 
   Size:        724     866
 
NspI r_CATG'y

Cuts at:      0    1137    1402    1590 
   Size:       1137     265     188

  Fragments arranged by size: 

                1137     265     188
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     247    1590 
   Size:        247    1343
 
PfoI T'CCnGG_A

Cuts at:      0     655     815    1590 
   Size:        655     160     775

  Fragments arranged by size: 

                 775     655     160
 
PmlI CAC'GTG

Cuts at:      0    1494    1590 
   Size:       1494      96
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1412    1444    1590 
   Size:       1412      32     146

  Fragments arranged by size: 

                1412     146      32
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     332     364    1590 
   Size:        332      32    1226

  Fragments arranged by size: 

                1226     332      32
 
PstI C_TGCA'G

Cuts at:      0      55     457    1590 
   Size:         55     402    1133

  Fragments arranged by size: 

                1133     402      55
 
PvuII CAG'CTG

Cuts at:      0     714     932    1590 
   Size:        714     218     658

  Fragments arranged by size: 

                 714     658     218
 
SacI G_AGCT'C

Cuts at:      0     965    1590 
   Size:        965     625
 
SfcI C'TryA_G

Cuts at:      0      51     144     453    1590 
   Size:         51      93     309    1137

  Fragments arranged by size: 

                1137     309      93      51
 
SphI G_CATG'C

Cuts at:      0    1402    1590 
   Size:       1402     188
 
StuI AGG'CCT

Cuts at:      0     511     607     874    1590 
   Size:        511      96     267     716

  Fragments arranged by size: 

                 716     511     267      96
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1305    1315    1590 
   Size:       1305      10     275

  Fragments arranged by size: 

                1305     275      10
 
TatI w'GTAC_w

Cuts at:      0     571    1590 
   Size:        571    1019
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     369     401    1590 
   Size:        369      32    1189

  Fragments arranged by size: 

                1189     369      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      69     233    1590 
   Size:         69     164    1357

  Fragments arranged by size: 

                1357     164      69
 
XmnI GAAnn'nnTTC

Cuts at:      0    1115    1590 
   Size:       1115     475
 

 Enzymes that do cut:

     AccI   Acc65I     ApoI     AvaI     BanI    BanII     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BsaAI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BstYI
    BtgZI     ClaI    CspCI     DraI     EaeI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI    HaeII    Hin4I     KpnI     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI    PflMI     PfoI
     PmlI     PpiI     PsrI     PstI    PvuII     SacI     SfcI     SphI
     StuI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BmtI    BpuEI     BsaI    BsaBI    BsaWI
    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     BtsI   DraIII     DrdI
     EagI     EarI     EciI EcoO109I     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     MluI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StyI
     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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