Restriction map for pF1KE1589
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       Acc65I      AccII       AflII       AhdI        
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BcgI        BciVI       BfrI        BglI        
BglII       BlnI        BsgI        BsiWI       BspDI       
BsrBI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     ClaI        
CpoI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoO109I    EcoO65I     
EcoRV       FseI        FspI        HincII      HindII      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MunI        MvnI        NarI        NdeI        
NheI        NotI        NruI        NspV        PacI        
PflFI       PmeI        PpuMI       PshAI       PshBI       
PvuI        RsrII       SacII       SalI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          748  GTCTAC               GT^[AC][GT]AC
AccIII       2113  TCCGGA               T^CCGGA
AclI           80  AACGTT               AA^CGTT
AcyI          307  GGCGTC               G[GA]^CG[CT]C
AflIII       1363  ACATGT               A^C[GA][CT]GT
AgeI         2753  ACCGGT               A^CCGGT
AlwNI        2600  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      2468  AGCGCT               AGC^GCT
BanI          990  GGCACC               G^G[CT][GA]CC
BbrPI         295  CACGTG               CAC^GTG
BclI          480  TGATCA               T^GATCA
BsaAI         295  CACGTG               [CT]AC^GT[GA]
BsaBI        1303  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         307  GGCGTC               G[GA]^CG[CT]C
BseAI        2113  TCCGGA               T^CCGGA
BspEI        2113  TCCGGA               T^CCGGA
BspHI        3023  TCATGA               T^CATGA
BspLU11I     1363  ACATGT               A^CATGT
DraI          606  TTTAAA               TTT^AAA
DsaI         2264  CCATGG               C^C[GA][CT]GG
Eco47III     2468  AGCGCT               AGC^GCT
EcoRI         374  GAATTC               G^AATTC
EcoT14I      2264  CCATGG               C^C[AT][AT]GG
EcoT22I      1849  ATGCAT               ATGCA^T
FauI          549  GCGGG                CCCGC(4/6)
FbaI          480  TGATCA               T^GATCA
HaeII        2468  AGCGCT               [GA]GCGC^[CT]
Hin1I         307  GGCGTC               G[GA]^CG[CT]C
MamI         1303  GATGTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2113  TCCGGA               T^CCGGA
MspA1I          5  CAGCTG               C[AC]G^C[GT]G
NaeI         1043  GCCGGC               GCC^GGC
NcoI         2264  CCATGG               C^CATGG
NgoMIV       1043  GCCGGC               G^CCGGC
NsiI         1849  ATGCAT               ATGCA^T
PaeR7I       2519  CTCGAG               C^TCGAG
PflMI        2415  CCATCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2753  ACCGGT               A^CCGGT
PmaCI         295  CACGTG               CAC^GTG
PmlI          295  CACGTG               CAC^GTG
Psp1406I       80  AACGTT               AA^CGTT
PstI         2606  CTGCAG               CTGCA^G
PvuII           5  CAGCTG               CAG^CTG
RcaI         3023  TCATGA               T^CATGA
SexAI        2225  ACCAGGT              A^CC[AT]GGT
SphI         1148  GCATGC               GCATG^C
StyI         2264  CCATGG               C^C[AT][AT]GG
Van91I       2415  CCATCTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          346  CCACCAGAGGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2519  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

Alw44I        965  GTGCAC               G^TGCAC
             1503  GTGCAC              
AocI         2332  CCTCAGG              CC^T[ACGT]AGG
             2880  CCTGAGG             
ApaLI         965  GTGCAC               G^TGCAC
             1503  GTGCAC              
AvaI          970  CCCGAG               C^[CT]CG[GA]G
             2519  CTCGAG              
BalI         1127  TGGCCA               TGG^CCA
             2894  TGGCCA              
BanII        2639  GAGCTC               G[GA]GC[CT]^C
             2986  GAGCTC              
BcnI         2152  CCGGG                CC^[GC]GG
             2644  CCCGG               
BsaI         2908  GAGACC               GGTCTC(1/5)
             3147  GAGACC              
BsaWI        2113  TCCGGA               [AT]^CCGG[AT]
             2753  ACCGGT              
BsiEI        1896  CGGTCG               CG[GA][CT]^CG
             2755  CGGTCG              
BsmBI         309  CGTCTC               CGTCTC(1/5)
             1164  CGTCTC              
BsmI          766  GAATGC               GAATGC(1/-1)
              941  GAATGC              
BsoBI         970  CCCGAG               C^[CT]CG[GA]G
             2519  CTCGAG              
Bsp1407I     1305  TGTACA               T^GTACA
             1626  TGTACA              
BsrFI        1043  GCCGGC               [GA]^CCGG[CT]
             2753  ACCGGT              
BsrGI        1305  TGTACA               T^GTACA
             1626  TGTACA              
BstAPI       1143  GCAAGGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2400  GCAAGTGTTGC         
Bsu36I       2332  CCTCAGG              CC^T[ACGT]AGG
             2880  CCTGAGG             
CfoI         2067  GCGC                 GCG^C
             2469  GCGC                
Cfr10I       1043  GCCGGC               [GA]^CCGG[CT]
             2753  ACCGGT              
EaeI         1127  TGGCCA               [CT]^GGCC[GA]
             2894  TGGCCA              
Eco81I       2332  CCTCAGG              CC^T[ACGT]AGG
             2880  CCTGAGG             
EcoNI        1229  CCTCAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1864  CCTTTTCAAGG         
HhaI         2067  GCGC                 GCG^C
             2469  GCGC                
HinP1I       2067  GCGC                 G^CGC
             2469  GCGC                
HindIII      1733  AAGCTT               A^AGCTT
             2707  AAGCTT              
MluNI        1127  TGGCCA               TGG^CCA
             2894  TGGCCA              
MscI         1127  TGGCCA               TGG^CCA
             2894  TGGCCA              
NciI         2152  CCGGG                CC^[GC]GG
             2644  CCCGG               
SacI         2639  GAGCTC               GAGCT^C
             2986  GAGCTC              
ScaI           49  AGTACT               AGT^ACT
             1720  AGTACT              
SmlI          890  CTCAAG               C^T[CT][GA]AG
             2519  CTCGAG              
SspBI        1305  TGTACA               T^GTACA
             1626  TGTACA              
XbaI          519  TCTAGA               T^CTAGA
             1608  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaII       BbsI        
BbvCI       BbvI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CelII       Cfr13I      DdeI        DpnII       EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HgaI        HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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